Relationship between Millitary College Students' Puzzles of Intercourse and Familial Function,Coping Style

目的 了解军校大学生的人际关系困扰状况及其与家庭功能和应对方式的相关.方法 选取石家庄市某军校765名一年级大学生为研究对象,使用McMaster家庭功能评定量表(FAD)、人际关系综合诊断量表和应对方式量表进行调查,通过相关分析和回归分析,探讨人际关系困扰与家庭功能和应对方式的相关性.结果 军校大学生人际困扰总检出率为31.80％;家庭功能和应对方式各因子和人际关系困扰均呈显著相关;Logistic回归分析显示,沟通、情感反应和总的功能以及应对方式的6个因子均进入回归方程.结论 军校大学生人际关系状况不容乐观,家庭功能和应对方式对人际因子状况有显著影响,要加强军校心理教育与人际关系主题辅导.     

Identification of an HLA-A*0201-restrictive CTL epitope from MUC4 for applicable vaccine therapy

Recent research has indicated that MUC4 plays an important role in the development of many tumors and may prove useful as a novel cancer immunotherapy target. We aimed to identify HLA-A*0201-restrictive cytotoxic T lymphocyte (CTL) epitopes of the cancer-associated antigen MUC4. The MUC4 sequence was scanned for immunogenic peptides using HLA-binding prediction software. Dendritic cells (DCs) from peripheral blood mononuclear cells (PBMCs) were induced by cytokines. Five possible CTL epitopes were selected by software analysis, synthesized, and used to pulse mature DCs. The CD8⁺ T cells from PBMCs from an HLA-A*0201 healthy donor were stimulated with autologous MUC4-peptide-loaded DCs and expanded in vitro. T cell activation was assessed by ELISPOT, and cytotoxicity was determined by ⁵¹chromium (⁵¹Cr)-release assays. Our results show that CTLs induced by peptide P01204 could lyse T2 cells pulsed with peptide P01204 and HCT-116 cells (MUC4⁺, HLA-A2⁺). Compared with a control peptide, P01204 increased the number of IFN-γ producing T cells. Overall, these results suggest that P01204 is a novel HLA-A*0201-restrictive CTL epitope of the cancer-associated antigen MUC4. This will provide a foundation for the development of tumor-specific peptide vaccines.     

补肾化淤汤治疗强直性脊柱炎肾虚血瘀证的效果及对患者疾病活动程度、BASFI评分的影响

目的 探讨补肾化淤汤治疗强直性脊柱炎(AS)肾虚血瘀证的效果及对患者疾病活动程度、Bath强直性脊柱炎功能指数(BASFI)评分的影响.方法 选取2018年6月至2020年12月我院接收的86例AS肾虚血瘀证患者进行研究,以治疗方案将其分为对照组与观察组,各43例.对照组采取常规西药治疗,观察组在对照组治疗基础上加施补...     

膜联蛋白A1拟肽Ac2-26对心肺转流大鼠肺损伤的影响

目的 通过观察Ac2-26对心肺转流(CPB)大鼠肺功能、肺组织结构及丝裂原活化蛋白激酶p38/细胞核因子-κB（p38MAPK/NF-κB）表达影响。
方法 选择成年健康SD雄性大鼠18只,体重350～450 g。随机分成三组：假手术组（S组）、缺血-再灌注组（IR组）和膜联蛋白A1（AnxA1）拟肽Ac2-26组（AC组）,每组6只。S组做穿刺及开胸处理,不建立CPB;IR组建立CPB,10 min后开胸夹闭左肺门;AC组建立CPB,在CPB前10 min给予Ac2-26 1 mg/kg,10 min后开胸夹闭左肺门。分别在CPB前、开放左肺门时、停CPB 90 min时,记录大鼠HR、MAP,并取动脉血行血气分析,计算氧合指数（OI）、呼吸指数（RI）。停CPB 90 min时用ELISA法检测左肺组织肿瘤坏死因子-α（TNF-α）和白细胞介素-10（IL-10）、支气管液肺泡灌洗液（BALF）中白细胞介素-6（IL-6）和总蛋白含量,采用Western-blot法检测肺组织丝裂原活化蛋白激酶p38（p38MAPK）、磷酸化丝裂原活化蛋白激酶p38（p-p38MAPK）、核因子-κBp65（NF-κBp65）、磷酸化核因子-κBp65（p-NF-κBp65）和AnxA1含量,取左肺组织行光镜检查和病理损伤评分。
结果 与S组比较,开放左肺门时、停CPB 90 min时IR组和AC组HR明显减慢,MAP、OI明显降低,RI明显升高（P<0.05）,停CPB 90 min时IR组和AC组TNF-α、IL-10、IL-6、总蛋白、p-p38MAPK、p-NF-κBp65、AnxA1含量明显升高（P<0.05）,病理损伤评分明显升高（P<0.05）。与IR组比较,停CPB 90 min时AC组HR明显增快,OI明显升高,RI明显降低（P<0.05）,TNF-α、IL-6、总蛋白、p-p38MAPK、p-NF-κBp65、AnxA1含量明显降低（P<0.05）,IL-10明显升高（P<0.05）,病理损伤评分明显降低（P<0.05）。
结论 膜联蛋白A1拟肽Ac2-26可抑制大鼠CPB后肺组织丝裂原活化蛋白激酶p38的活化,抑制核因子-κB的分泌,降低TNF-α和IL-6的含量,增加IL-10含量,减轻其肺损伤程度,减少炎性渗出,改善肺功能。     

Noninvasive high-speed photoacoustic tomography of cerebral hemodynamics in awake-moving rats

We present a noninvasive method of photoacoustic tomography (PAT) for imaging cerebral hemodynamics in awake-moving rats. The wearable PAT (wPAT) system has a size of 15 mm in height and 33 mm in diameter, and a weight of ~8 g (excluding cabling). The wPAT achieved an imaging rate of 3.33 frames/s with a lateral resolution of 243 μm. Animal experiments were designed to show wPAT feasibility for imaging cerebral hemodynamics on awake-moving animals. Results showed that the cerebral oxy-hemoglobin and deoxy-hemoglobin changed significantly in response to hyperoxia; and, after the injection of pentylenetetrazol (PTZ), cerebral blood volume changed faster over time and larger in amplitude for rats in awake-moving state compared with rats under anesthesia. By providing a light-weight, high-resolution technology for in vivo monitoring of cerebral hemodynamics in awake-behaving animals, it will be possible to develop a comprehensive understanding on how activity alters hemodynamics in normal and diseased states.     

胰腺神经内分泌肿瘤的外科治疗

胰腺神经内分泌肿瘤(PNEN)分为功能性和无功能性两大类, 根治性手术是治愈肿瘤和争取患者长期生存的唯一有效手段。局部可切除肿瘤首选手术切除, 手术方式包括局部切除(剜除)术和规则性胰腺切除术。胰岛素瘤和直径小于2 cm无功能性PNEN可行局部切除(剜除)术、保留脾脏的胰体尾切除术或节段胰腺切除术; 直径2 cm及以上或恶性倾向PNEN应行根治性切除术, 同时附加区域淋巴结清扫术, 包括胰十二指肠切除术、胰体尾切除术及中段胰腺切除术等。对于局部进展无法根治切除病变, 功能性PNEN可以选择减瘤术, 尽量切除90%以上的肿瘤, 包括转移灶和原发灶; 无功能性PNEN患者出现黄疸、消化道梗阻和出血等并发症时, 可考虑行肿瘤原发灶切除。肝脏是PNEN最常见远处转移部位, 可分为Ⅰ、Ⅱ、Ⅲ型。对Ⅰ型PNEN患者, 只要无手术禁忌应建议手术切除。对Ⅱ型PNEN患者如考虑行减瘤术, 应尽可能切除全部肝转移灶的90%;对原发灶已切除且不伴有肝外远处转移、分化好(G1/G2) 的患者, 肝移植可以作为一种治疗选择。对无法切除的Ⅲ型PNEN患者, 可选择多种辅助治疗方法。     

Seamline optimization based on ground objects classes for orthoimage mosaicking

Seamline determination is one of the key steps in orthoimage mosaicking. To avoid discontinuity in mosaic, this letter presents a seamline optimization method based on ground objects classes. The presented method classifies ground objects in overlaps of adjacent images into three classes, that is, buildings, vegetation and other classes, using normalized difference vegetation index and morphological building index. Based on ground objects classes, three types of regions, that is, obstacle regions, preferred regions and general regions, are further formed. Then, each type of region is assigned a different weight to optimize obtained pixel costs. Finally, a path finding with limiting level of maximum cost and differential cost is carried out to search the shortest path from start point to end point based on optimized pixel costs in overlaps as the final seamline. Experimental results including comparison with other methods demonstrate the potential of the presented method.     

甲基莲心碱逆转人结肠癌细胞奥沙利铂耐药的体外研究

目的:探讨甲基莲心碱(Nef)对人结肠癌细胞奥沙利铂(OXA)耐药的逆转作用及机制。方法:采用OXA浓度逐步递增法(2、4、8、12、24、48μmol/L)孵育人结肠癌HCT116细胞诱导构建OXA耐药株HCT116/OXA;检测Nef对HCT116/OXA细胞的细胞毒性,确定Nef的最适作用浓度和时间;分析并比较OXA(IC_(50)浓度)单独处理、Nef(最适作用浓度)单独处理、OXA(IC_(50)浓度)联合Nef(最适作用浓度)处理后,HCT116/OXA细胞的增殖,凋亡情况及凋亡相关蛋白(Bcl-2,Bax,PARP,p-PARP)的表达情况。结果:与亲本HCT116细胞比较,HCT116/OXA细胞较对OXA的耐药性明显增高(IC_(50):21.00μmol/Lvs.112.00μmol/L,P0.05),耐药指数为5.33。Nef能明显抑制HCT116/OXA的增殖有作用(P0.05),并呈浓度依赖性,其最适作用浓度、时间分别为5μmol/L、24h(细胞存活率为90%)。与OXA单独处理比较,HCT116/OXA细胞对OXA联合Nef处理的耐受性明显降低(IC_(50):112.00μmol/Lvs.45.47μmol/L,P0.05),逆转倍数为2.46;Nef单独作用对HCT116/OXA细胞的凋亡影响不明显(P0.05),但其与OXA联合作用对HCT116/OXA细胞凋亡诱导作用明显强于OXA单独作用(P0.05);与OXA或Nef单独作用比较,OXA联合Nef作用后,HCT116/OXA细胞抗凋亡蛋白Bcl-2表达明显下降,Bax、p-PARP等凋亡蛋白表达明显上升(均P0.05)。结论:Nef可逆转HCT116/OXA对OXA的耐药,机制可能与其调节Bcl-2/Bax表达水平,从而与OXA产生协同作用有关。     

重组骨保护素（rhOPG)药物的研究现状及应用展望

破骨细胞分化因子(receptor activator nuclear factor kappa B ligand,RANKL)与骨保护素(osteoprotegefin,OPG)是调节破骨细胞分化和骨吸收功能的关键因子.RANKL是连接骨与免疫系统的破骨细胞生成因子,能刺激破骨细胞分化和发挥骨吸收功能;OPG作为RANKL的诱饵受体,能够阻止RANKL与RANK的结合,从而抑制破骨细胞的分化和激活.应用重组骨保护素OPG融合蛋白可以预防和治疗骨丢失类疾病,抑制牙周炎发病过程中牙槽骨吸收,并成为目前预防微重力相关的骨损失的最有希望的药物之一.笔者对重组人骨保护素(rhOPG)基因工程药物的研究现状进行了简要的综述.     

建立兔单纯腹壁爆炸伤合并内脏器官外露的动物模型

目的建立可进行长期观察的单纯腹壁爆炸伤合并内脏器官外露的动物模型,观察此类创伤的致伤和转归规律。方法 8～10周龄生长期白家兔12只,随机分为2组,每组6只。全身麻醉条件下,标记致伤部位。A组采用自制腹腔防护装置埋入腹腔,然后采用0.125 g TNT当量炸药球贴于致伤部位致伤;B组不经防护处理直接采用同样爆源致伤。伤后观察各组大体形态,对活体动物每日取伤部组织常规HE染色,切片观察;死亡动物记录生存时间并行尸检。结果两组动物致伤部位腹壁呈全层开放性爆炸伤。A组实验动物创面面积(3.9±0.56)cm2;B组创伤面积(3.83±0.49)cm2,两组间无显著差异(P0.05)。A组实验动物存活时间为(141±87.78)h。B组实验动物平均存活时间为(3.67±2.25)h,两组实验动物存活时间存在明显差异(P0.05)。尸检和病理观察发现,对照组动物早期死亡原因为内脏损伤和腹腔内出血,实验组发生内脏纤维粘连未见明显创伤。腹壁各层组织受爆炸的创伤有差异,呈现"外轻内重"的病理变化,并呈进行性发展,尤以肌肉组织的变化最为典型。结论本实验模拟了腹部爆炸伤致伤机制。经有效防护,建立了可用于长期观察的单纯腹壁爆炸伤动物模型。