全文获取类型
收费全文 | 615篇 |
免费 | 41篇 |
专业分类
耳鼻咽喉 | 1篇 |
儿科学 | 7篇 |
妇产科学 | 10篇 |
基础医学 | 68篇 |
口腔科学 | 7篇 |
临床医学 | 37篇 |
内科学 | 143篇 |
皮肤病学 | 1篇 |
神经病学 | 218篇 |
特种医学 | 14篇 |
外科学 | 64篇 |
综合类 | 2篇 |
预防医学 | 15篇 |
眼科学 | 9篇 |
药学 | 19篇 |
肿瘤学 | 41篇 |
出版年
2024年 | 2篇 |
2023年 | 5篇 |
2022年 | 13篇 |
2021年 | 28篇 |
2020年 | 10篇 |
2019年 | 18篇 |
2018年 | 16篇 |
2017年 | 8篇 |
2016年 | 9篇 |
2015年 | 10篇 |
2014年 | 18篇 |
2013年 | 23篇 |
2012年 | 29篇 |
2011年 | 28篇 |
2010年 | 18篇 |
2009年 | 15篇 |
2008年 | 31篇 |
2007年 | 33篇 |
2006年 | 28篇 |
2005年 | 25篇 |
2004年 | 28篇 |
2003年 | 38篇 |
2002年 | 24篇 |
2001年 | 24篇 |
2000年 | 21篇 |
1999年 | 34篇 |
1998年 | 12篇 |
1997年 | 13篇 |
1996年 | 4篇 |
1995年 | 7篇 |
1994年 | 6篇 |
1993年 | 3篇 |
1992年 | 8篇 |
1991年 | 10篇 |
1990年 | 5篇 |
1989年 | 3篇 |
1988年 | 10篇 |
1987年 | 10篇 |
1986年 | 4篇 |
1985年 | 3篇 |
1984年 | 5篇 |
1983年 | 4篇 |
1981年 | 2篇 |
1978年 | 2篇 |
1974年 | 1篇 |
1972年 | 1篇 |
1971年 | 1篇 |
1969年 | 1篇 |
1968年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有656条查询结果,搜索用时 15 毫秒
101.
102.
103.
Nobuko Shiraiwa Akiko Ishii Hiroyuki Iwamoto Hidehiro Mizusawa Yasuo Kagawa Shigeo Ohta 《Journal of the neurological sciences》1993,120(2):174-179
A point mutation of mitochondrial tRNALeu(UUR) gene is responsible for a MELAS (mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes) subgroup of mitochondrial encephalomyopathies. In most cases, the mutant mitochondrial DNA (mtDNA) coexists with normal mtDNA in a heteroplasmic manner. In order to quantify the content of mutant mtDNA, we developed a quantitative method of PCR. Using this method, the distribution of the mutant mtDNA was examined in 32 different tissues among 18 autopsied organs from a patient with MELAS, who had shown hypophyseal dysfunction. The percentage of the mutant mtDNA at nucleotide number 3243 in each tissue was ranged between 22% and 95%. The content of the mutant mtDNA was at the highest (95%) in the hypophysis and higher in the cerebral cortex than in the white matter. This study shows a possible correlation of tissue dysfunction with accumulation of the mutant mtDNA within the brain. 相似文献
104.
105.
106.
Anat Milman Antoine Andorin Pieter G. Postema Jean-Baptiste Gourraud Frederic Sacher Philippe Mabo Sung-Hwan Kim Shingo Maeda Yoshihide Takahashi Tsukasa Kamakura Takeshi Aiba Giulio Conte Jimmy J.M. Juang Eran Leshem Yoav Michowitz Rami Fogelman Aviram Hochstadt Yuka Mizusawa Bernard Belhassen 《Heart rhythm》2019,16(10):1468-1474
107.
Iuchi Y Torimoto Y Sato K Tamura Y Jimbo J Inamura J Shindo M Ikuta K Ohnishi K Kohgo Y 《International journal of hematology》2006,84(5):449-458
We have reported that immunotherapy using leukemia cell-derived heat shock proteins (HSPs) is effective against minimal residual disease (MRD) after syngeneic stem cell transplantation (SCT) in mice. However, leukemia patients after SCT are usually immunocompromised and immunologically tolerant to leukemia cells. We investigated whether the use of dendritic cells (DCs) in combination with HSP70 enhances cytotoxicity against B-cell leukemia cell line A20 in mice after syngeneic SCT. All unimmunized mice died of leukemia early after A20 cell inoculation, whereas mice immunized with HSP70 or HSP70-pulsed DCs survived significantly longer. Although only 60% of the HSP70-immunized mice survived, all mice immunized with HSP70-pulsed DCs survived without MRD. In addition, the cytotoxicities against A20 cells for splenocytes from mice immunized with HSP70-pulsed DCs were significantly higher than those of HSP70-immunized mice, and the cytotoxicities against A20 cells were significantly blocked by anti-CD8 antibody and by major histocompatibility complex class I antibody, but not by anti-CD4 antibody. Moreover, abnormalities were detected in neither the biochemical data nor the histopathologic findings. These findings indicate that the combined use of DCs and leukemia cell-derived HSP70 enhances the antileukemia effect by inducing the specific cytotoxicities of CD8+ cytotoxic T-cells, thereby eradicating MRD effectively and safely, even in an immunocompromised state after syngeneic SCT. This approach may thus be useful for further application of HSP in leukemia patients after autologous SCT. 相似文献
108.
109.
Mizuochi T Okada Y Umemori K Mizusawa S Yamaguchi K 《Journal of virological methods》2006,136(1-2):254-256
Genetic variability of the hepatitis B virus (HBV) constitutes one of the major challenges for diagnosis of HBV infection. It is plausible that amino acid substitutions in the "a" determinant of the HBV surface antigen (HBsAg) that affect antigenic sites, whether originating from genetic diversity or from mutations in the HBV strain itself, will affect the sensitivity of some diagnostic kits. In fact, recent studies have indicated that some diagnostic kits had false negative results with particular HBsAg mutants. There have been, however, few substantial studies evaluating sensitivities of diagnostic kits to the HBsAg encoded by different HBV genotypes. Our recent study found that 10 diagnostic kits available in Japan were able to detect HBsAg irrespective of whether it originated from HBV genotypes A, B or C, with the latter two genotypes being the dominant species in East Asia. In this study, we extended our previous efforts by assessing the ability of diagnostic kits to detect recombinant HBsAg derived from HBV genotypes A to H. Our results demonstrated that 9 out of 10 diagnostic kits evaluated were able to detect as low as 0.2 International Units (IU)/ml HBsAg, irrespective of HBV genotype. The genotypic differences in the HBV family thus appear to have little impact on the sensitivity of currently available HBsAg diagnostic kits. 相似文献
110.
Daisuke Takahari Narikazu Boku Junki Mizusawa Atsuo Takashima Yasuhide Yamada Takayuki Yoshino Kentaro Yamazaki Wasaburo Koizumi Kazutoshi Fukase Kensei Yamaguchi Masahiro Goto Tomohiro Nishina Takao Tamura Akihito Tsuji Atsushi Ohtsu 《The oncologist》2014,19(4):358-366