mmp-14和CD34在青年乳腺癌演变过程中的表达及意义

目的：研究基质金属蛋白酶14（mmp-14）与CD34在≤35岁乳腺癌、乳腺良性肿瘤、乳腺增生症及乳腺癌旁组织患者中的表达及临床意义。方法采用免疫组织化学方法对30例乳腺癌、30例乳腺良性肿瘤、30例乳腺增生症及30例癌旁组织中乳腺终末导管及腺泡上皮细胞患者的mmp-14表达情况及CD34标记的间质血管数目进行检测。结果 mmp-14在4组导管及腺泡上皮细胞中的表达情况：乳腺癌组mmp-14高表达,与其他3组两两比较差异具统计学意义（P＜0.05）。CD34在4组中标记间质血管数目的情况：乳腺增生症组和乳腺癌组血管增生数目均高于良性肿瘤组,差异有统计学意义（P＜0.05）。结论乳腺终末导管及腺泡上皮细胞mmp-14的高表达和CD34标记的间质血管数目增加与青年乳腺癌的演变过程密切相关,有望成为青年乳腺癌早期诊断和治疗可靠的临床指标。     

头孢哌酮 /三唑巴坦复方制剂的人体药代动力学研究

目的研究静脉滴注头孢哌酮/三唑巴坦复方制剂(800mg/200mg)与头孢哌酮(800mg)在健康志愿者中的药代动力学。方法10名健康志愿者分别随机交叉静脉滴注头孢哌酮/三唑巴坦复方制剂1600mg/400mg和对照药头孢哌酮1600mg,用反相高效液相色谱法分别测定头孢哌酮和三唑巴坦血药浓度和尿中的药物浓度,计算两药的药代动力学参数,比较复方制剂两组分有无药代动力学的相互作用。结果试验药和参比药中头孢哌酮的血药峰浓度Cmax分别为(181.52±39.94)、(176.21±32.66)mg/L;消除半衰期t1/2β分别为(2.23±0.67)、(2.28±0.61)h;药时曲线下面积AUC0.5-12分别为(351.96±54.53)、(336.31±54.93)mg·h/L,AUC0-12分别为(402.21±57.6)、(376.64±55.18)mg·h/L,AUC0-inf分别为(413.47±59.06)、(395.83±62.08)mg·h/L;24h尿药累积排泄百分率分别为(22.92±7.79)%、(21.00±7.33)%。三唑巴坦的峰浓度Cmax为(20.31±4.76)mg/L,药时曲线下面积AUC0.5-5为(12.96±2.40)mg·h/L,AUC0-5为(18.45±3.52)mg·h/L,AUC0-inf为(19.42±3.67)mg·h/L;消除半衰期t1/2β为(0.84±0.28)h;24h尿药累积排泄百分率为(60.56±19.37)%。结论两种制剂中,头孢哌酮的主要药代动力学参数Cmax、t1/2β及AUC经双单侧检验无显著性差异(P>0.05),三唑     

Tolerability,pharmacokinetics and pharmacodynamics of CMAB001, an anti-CD11a antibody,in Chinese healthy volunteers and psoriatic patients

Aim:

To evaluate the pharmacokinetics (PK), pharmacodynamics (PD) and primary tolerability of an anti-CD11a monoclonal antibody (CMAB001) in Chinese healthy volunteers and psoriatic patients.

Methods:

Two open-label studies were conducted. One was a parallel-group, single-center, dose-escalation test, including 24 healthy adult volunteers from 18 to 45 years in age. All subjects randomly received a single subcutaneous injection dose of 0.5, 1.0 or 2.0 mg/kg. The other was a multiple-dose study: 10 adult psoriatic patients were administered weekly subcutaneous injections of 1.0 mg/kg for 7 weeks.

Results:

CMAB001 was well tolerated in the single- and multiple-dose studies. Slow absorption was observed in both studies. In the single-dose study, the concentration of CMAB001 reached its highest level 2 d later after the injection, and the C_max increased in an approximate dose-proportionate manner, while the area under curve (AUC) showed much greater than dose-proportionate increase. In the multiple-dose study, the steady-state serum concentration level was attained following the 4th injection.

Conclusion:

CMAB001 exhibited a nonlinear pharmacokinetic profile over the dose range from 0.5 to 2.0 mg/kg, and was well tolerated in healthy volunteers and psoriatic patients.     

核糖核酸抗肝纤维化的实验与临床研究

为研究核糖核酸（RNA）抗肝纤维化的作用，用二甲基亚硝胺（DMN）诱发大鼠肝纤维化，由光镜和电镜检查结果，RNA治疗组肝细胞坏死、变性及胶原纤维增生程度较病理对照组及辅酶Q10治疗组均明显减轻。随机对照用RNA和辅酶Q10分别治疗慢性肝炎（CH）伴／不伴代偿期肝硬化（HC）93例及92例，疗程3个月。结果RNA组改善肝功能和降低血清三型前胶原（PCI）和透明质酸（HA）的疗效均明显优于辅酶Q10组。肝活俭显示，RNA治疗后肝脏炎症活动度和胶原纤维含量较治疗前明显减轻，而辅酶Q10治疗者反有加重。实验与临床研究结果一致，说明RNA有抗肝纤维化作用。     

社交媒体时代企业社会责任实践的机遇与挑战

近年来兴起的社交媒体已经超出了“社交”功能,并逐渐在商业、行政、教育等领域得到越来越广泛的应用.企业社会责任的履行已被视为企业实现可持续发展的重要途径,认识社交媒体对企业社会责任活动的影响有助于企业提升社会责任活动的效果.该文探讨了社交媒体的应用给企业社会责任实践带来的机遇与挑战,以促进企业在社会责任实践中合理、高效地运用社交媒体,获取可持续竞争优势.     

白蛋白微球作为肝靶向给药载体的研究

用均匀设计方法和计算机技术筛选了乳化化学交联法制备白蛋白微球的六个因素,十二个水平。优化出最佳制备工艺,制备了平均粒径0.41～0.47μm的白蛋白微球。将此工艺制备的¹²⁵I-白蛋白微球做动物体内研究,结果表明微球iv后主要浓集在肝脏,可达注入总剂量的68%,此微球在靶组织肝脏的变化规律可用二室模型契合。     

NIR light-induced tumor phototherapy using photo-stable ICG delivery system based on inorganic hybrid

NIR responsive inorganic hybrid (Ti@GO) was synthesized. It could absorb NIR light and convert it into local hyperthermia and ROS synchronously. Ti@GO was firstly developed as a photosensitizer and a photothermal agent to realize tumor PTT and PDT. For anti-tumor application, HA was grafted on Ti@GO simultaneously as water solubility improver and tumor targeting moiety. ICG was chosen as a model drug. Results demonstrated that HA-Ti@GO could remarkably improve ICG stability and drug accumulation in 4T1 cells, enhance tumor phototherapy efficiency and reduce light-associated side effects. HA-Ti@GO/ICG under NIR laser irradiation showed a significant decreased cell viability of 20.7 ± 2.6% and a high DNA damage degree of 82.4 ± 8.3%. Moreover, in vivo results showed that HA-Ti@GO/ICG plus NIR laser achieved almost complete tumor regression on 4T1 tumor-bearing mice, with a tumor volume of 67.0 mm³. Taken together, our study provided a promising strategy to realize synergistic PTT/PDT tumor therapy with a single NIR light.     

天然色素的开发应用

目的介绍天然色素的种类、提取分离方法及应用现状,为进一步开发和利用天然色素提供理论依据。方法查阅相关文献,对天然色素的种类、提取分离方法及其应用等方面进行归纳分析。结果天然色素种类繁多,其提取方法有溶剂提取法、超临界流体萃取法、超声波提取法、微波提取法、酶法、空气爆破法,分离方法有溶液分离法、膜分离法、柱层析法、薄层色谱法、高效液相色谱法、离心液相色谱法、高速逆流色谱法,主要应用于食品、化妆品、药品和纺织品着色等方面。结论天然色素自身具有营养价值,毒副作用小,可取代合成色素应用于食品和化妆品的着色,并具有一定药理作用,进一步开发利用的意义深远。     

TNF-α对人肺癌A549细胞凋亡的影响

目的探讨TNF-α对人肺癌A549细胞凋亡的影响;方法培养人肺癌A549细胞,与TNF-α(10、100U·ml-1)共孵育,通过Western blot分析P53蛋白表达的变化,同时利用Annexin V/PI双染色流式细胞仪,检测人肺癌A549细胞凋亡率的变化。结果与空白组相比,TNF-α组的P53蛋白表达明显升高,且细胞凋亡率也明显升高,分别为1.22±0.62和1.65±0.38(P<0.01)。结论TNF-α可以诱导人肺癌A549细胞凋亡,从而可能起到抗肿瘤的疗效。     

心脏靶向超声造影剂空气白蛋白微球的研究(1)

按正交设计筛选了用发射超声能量合成白蛋白空气微球的较佳制备工艺,并对微球的形态、粒径与粒径分布、稳定性、安全性和显影效果进行了系统研究。结果表明：微球的平均直径为 ４．２±０． ２ μｍ,平均浓度为（３．５±１．２）×１０８／ｍｌ。９２．５％的微球直径小于１０μｍ,微球在２℃～５℃条件下能稳定储存１２个月,经兔外用静脉注射后能顺利通过肺微循环到达左心室致使左心室靶向超声显影。