全文获取类型
收费全文 | 75864篇 |
免费 | 6445篇 |
国内免费 | 3113篇 |
专业分类
耳鼻咽喉 | 641篇 |
儿科学 | 1336篇 |
妇产科学 | 1332篇 |
基础医学 | 8648篇 |
口腔科学 | 1489篇 |
临床医学 | 9137篇 |
内科学 | 13815篇 |
皮肤病学 | 1288篇 |
神经病学 | 4316篇 |
特种医学 | 1933篇 |
外国民族医学 | 19篇 |
外科学 | 8186篇 |
综合类 | 10065篇 |
现状与发展 | 7篇 |
一般理论 | 9篇 |
预防医学 | 5729篇 |
眼科学 | 1811篇 |
药学 | 7224篇 |
72篇 | |
中国医学 | 3455篇 |
肿瘤学 | 4910篇 |
出版年
2024年 | 200篇 |
2023年 | 1027篇 |
2022年 | 2301篇 |
2021年 | 3792篇 |
2020年 | 2735篇 |
2019年 | 2492篇 |
2018年 | 2756篇 |
2017年 | 2425篇 |
2016年 | 2175篇 |
2015年 | 3230篇 |
2014年 | 4148篇 |
2013年 | 4442篇 |
2012年 | 6598篇 |
2011年 | 6819篇 |
2010年 | 4426篇 |
2009年 | 3751篇 |
2008年 | 4677篇 |
2007年 | 4565篇 |
2006年 | 4150篇 |
2005年 | 3834篇 |
2004年 | 2868篇 |
2003年 | 2681篇 |
2002年 | 2216篇 |
2001年 | 936篇 |
2000年 | 864篇 |
1999年 | 829篇 |
1998年 | 582篇 |
1997年 | 556篇 |
1996年 | 436篇 |
1995年 | 387篇 |
1994年 | 366篇 |
1993年 | 233篇 |
1992年 | 233篇 |
1991年 | 199篇 |
1990年 | 203篇 |
1989年 | 159篇 |
1988年 | 148篇 |
1987年 | 125篇 |
1986年 | 115篇 |
1985年 | 107篇 |
1984年 | 66篇 |
1983年 | 54篇 |
1982年 | 73篇 |
1981年 | 80篇 |
1980年 | 39篇 |
1979年 | 55篇 |
1978年 | 23篇 |
1977年 | 34篇 |
1976年 | 34篇 |
1975年 | 20篇 |
排序方式: 共有10000条查询结果,搜索用时 531 毫秒
21.
Platelet function has been described by many laboratory assays, and PL-11 is a new point-of-care platelet function analyzer based on platelet count drop method, which counts platelet before and after the addition of agonists in the citrated whole blood samples. The present study sought to compare PL-11 with other three major more established assays, light transmission aggregometry (LTA), VerifyNow? aspirin system and thromboelastography (TEG), for monitoring the short-term aspirin responses in healthy individuals. Ten healthy young men took 100?mg/d aspirin for 3-day treatment. Platelet function was measured via PL-11, LTA, VerifyNow and TEG, respectively. The blood samples were collected at baseline, 2 hour, 1 day during the aspirin treatment and 1 day, 5?±?1 days, 8?±?1 days after the aspirin withdrawal. Moreover, 90 additional healthy subjects were recruited to establish a reference range for PL-11. Platelet function of healthy subjects decreased significantly 2 hours after 100?mg/d aspirin intake and began to recover during 4–6 days after the aspirin withdrawal. Correlations between methods were PL-11 vs. LTA (r?=?0.614, p?<?0.01); PL-11 vs. VerifyNow (r?=?0.829, p?<?0.01); PL-11 vs. TEG (r?=?0.697, p?<?0.001). There was no significant bias between PL-11 and LTA at baseline (bias?=?1.94%, p?=?0.804) using Bland-Altman analysis, while the data of PL-11 were significantly higher than LTA (bias?=?24.02%, p?<?0.001) during the aspirin therapy. The reference range for PL-11 in healthy young individuals was from 66.8 to 90.5% (95%CI). When aspirin low-responsiveness was defined as LTA?>?20%, the cut-off values for each method were, respectively: PL-11?>?50%, VerifyNow?>?533 ARU, TEG?>?60.2%. The results of different platelet function assays were uninterchangeable for monitoring aspirin response and correlations among them were also varied. Correlations among PL-11 and other three major assays suggested the ability of PL-11 to assess the treatment effects of aspirin. But a large cohort study is needed to confirm the cut-off value of aspirin response detected by PL-11. 相似文献
22.
23.
骨碎补是历代临床常用中药,具有疗伤止痛、补肾强骨、消风祛斑等功效。其主要含黄酮、苯丙素、三萜、酚酸及其苷等类化学成分,现代研究表明骨碎补具有抗骨质疏松、促进骨折愈合、促软骨再生、护牙健齿、保护肾功能、抗炎、防治中毒性耳聋、降血脂等多种生物活性,开发前景广阔。本文对近年来骨碎补的化学成分、药理作用及临床应用研究进行综述,以期为骨碎补的进一步深入系统的研究和开发利用提供依据。 相似文献
24.
Enrique Luengo Izaskun Buendia Cristina Fernndez‐Mendívil Paula Trigo‐Alonso Pilar Negredo Patrycja Michalska Borja Hernndez‐García Cristina Snchez‐Ramos Juan A. Bernal Tsuneya Ikezu Rafael Len Manuela G. Lpez 《Journal of pineal research》2019,67(1)
Alterations in autophagy are increasingly being recognized in the pathogenesis of proteinopathies like Alzheimer's disease (AD). This study was conducted to evaluate whether melatonin treatment could provide beneficial effects in an Alzheimer model related to tauopathy by improving the autophagic flux and, thereby, prevent cognitive decline. The injection of AAV‐hTauP301L viral vectors and treatment/injection with okadaic acid were used to achieve mouse and human ex vivo, and in vivo tau‐related models. Melatonin (10 μmol/L) impeded oxidative stress, tau hyperphosphorylation, and cell death by restoring autophagy flux in the ex vivo models. In the in vivo studies, intracerebroventricular injection of AAV‐hTauP301L increased oxidative stress, neuroinflammation, and tau hyperphosphorylation in the hippocampus 7 days after the injection, without inducing cognitive impairment; however, when animals were maintained for 28 days, cognitive decline was apparent. Interestingly, late melatonin treatment (10 mg/kg), starting once the alterations mentioned above were established (from day 7 to day 28), reduced oxidative stress, neuroinflammation, tau hyperphosphorylation, and caspase‐3 activation; these observations correlated with restoration of the autophagy flux and memory improvement. This study highlights the importance of autophagic dysregulation in tauopathy and how administration of pharmacological doses of melatonin, once tauopathy is initiated, can restore the autophagy flux, reduce proteinopathy, and prevent cognitive decline. We therefore propose exogenous melatonin supplementation or the development of melatonin derivatives to improve autophagy flux for the treatment of proteinopathies like AD. 相似文献
25.
Maria Gonzalez-Cao Cristina Carrera Juan Francisco Rodriguez Moreno Pedro Rodríguez-Jiménez Mónica Antoñanzas Basa Rosa Feltes Ochoa Teresa Puertolas Eva Muñoz-Couselo José Luis Manzano Ivan Marquez-Rodas Juan Martín-Liberal Ainara Soria Pilar Lopez Criado Almudena Garcia-Castaño Aram Boada Pablo Ayala de Miguel Susana Puig Guillermo Crespo Alfonso Berrocal 《Journal of the American Academy of Dermatology》2021,84(5):1412-1415
26.
27.
Keyang Qian Hanqing Qian Juan Cai Wuheng Yue Xiaoxiao Yu Baorui Liu 《Pathology, research and practice》2019,215(4):755-760
Gastric cancer, one of the most common disease, has become a major public health problem worldwide. Cisplatin (DDP) has been a widely used drug for the treatment of cancer, also usually applied in gastric cancer in clinic. However, the side effects including toxicity and drug-resistance restricted the usage of DDP in clinic, so we prepared a DDP-complexed hydrogel (DDP-Gel) and investigated its efficacy in gastric cancer. For in vivo studies, MKN45-Luc cells were injected into BLAB/C node mice subcutaneously to establish gastric cancer with orthotopically grown tumors. Mice bearing tumors were treated with normal saline, DDP and DDP-Gel. Body weight and survival condition were observed and recorded. The treatment efficacy in vivo was detected by luciferase imaging and histological evaluation was performed by H&E staining of different organs. Additionally, normal ICR mice were treated with different doses of DDP/DDP-Gel to calculate their LD50 in vivo. The results showed that DDP-Gel prolonged survival time and ameliorated body weight changes of mice bearing tumors. DDP-Gel exhibited higher efficacy to inhibit tumor growth and metastasis, compared to DDP. Besides, LD50 of DDP-Gel was 166.0?mg/kg, 13.2 folds higher than DDP. As a conclusion, DDP-Gel showed a more effective and safer function than DDP in gastric cancer, which indicating that DDP-Gel might be a novel strategy for gastric cancer therapy. 相似文献
28.
30.
目的 研究血清窖蛋白-1 (Cav-1) 在慢性阻塞性肺疾病 (COPD) 相关肺动脉高压 (PAH) 患者中的表达及其意义。方法 选取稳定期COPD患者65例, 根据是否合并PAH分成COPD组 [肺动脉收缩压 (PASP) <40 mmHg, 35例] 及COPD-PAH组 (PASP ≥40 mmHg, 30例)。另选取在本院健康体检的志愿者30例作为对照组。对比各组基线资料、 动脉血气分析、 肺功能指标, 以及血清Cav-1、 白细胞介素 (IL) -6和肿瘤坏死因子-α (TNF-α) 的表达水平。绘制受试者工作特征 (ROC) 曲线, 评价Cav-1对COPD合并PAH的诊断价值。结果 COPD-PAH组与COPD组第一秒用力呼吸容积 (FEV1) /用力肺活量 (FVC)、 FEV1占预计值百分比 (FEV1%) 及氧分压 [p (O2 )] 低于对照组, 而二氧化碳分压 [p (CO2 )]、 PASP均高于对照组 (P<0.01)。COPD-PAH组p (O2 ) 低于COPD组, p (CO2 )、 PASP均高于COPD 组 (P<0.01)。对照组、 COPD组及COPD-PAH组Cav-1表达水平呈逐渐降低趋势, 而IL-6、 TNF-α表达水平呈逐渐升高趋势 (P<0.01)。血清Cav-1诊断COPD合并PAH的ROC曲线下面积为0.902 (0.821~0.955), 最佳截断值为 6.66 μg/L, 此时诊断敏感度为76.7%, 特异度为85.7%, 与多普勒超声诊断仪测量PASP结果比较一致性较好 (Kappa 值=0.627)。结论 血清Cav-1在COPD相关PAH患者表达明显下调, 可以作为预测COPD相关PAH的新型血清标志物。 相似文献