CD8+淋巴细胞表达强度与乙型病毒性肝炎不同肝组织炎症分级的关系

目的探讨CD8 淋巴细胞与乙型肝炎患者肝组织炎症分级的关系和意义。方法应用免疫组织化学技术,检测不同炎症分级的肝组织中CD8 淋巴细胞的表达。结果G0肝组织中CD8 表达弱,随着炎症分级的增强,CD8 表达逐渐增强。G4最为明显,有明显统计学意义。结论CD8 淋巴细胞表达强度与肝组织炎症分级有明显相关性。     

A New Prognostic Model Covering All Stages of Intrahepatic Cholangiocarcinoma

Background and Aims: Intrahepatic cholangiocarcinoma (ICC) is the second most common primary hepatic malig-nancy that causes a poor survival. We aimed to identi...     

急性淋巴细胞白血病患儿Bim及c-Myc表达水平与糖皮质激素敏感性的关系

目的 探讨ALL患儿Bim及c-Myc蛋白表达水平与糖皮质激素(GC)敏感性的关系,研究Bim 表达水平是否可作为判断早期GC敏感性的客观指标,从而指导临床治疗和判断预后.方法 采用Western blot方法检测1 μmol·L-1地塞米松作用于人T-ALL GC敏感细胞株(CEM-C7)和GC耐药细胞珠(CEM-C1) 0 h、12 h、24 h、36 h、48 h及72 h后Bim和c-Myc蛋白表达的变化;以30例初诊ALL患儿为研究对象,采用Western blot分析ALL患儿GC诱导前、GC诱导后24 h、48 h、72 h以及第8天Bim蛋白表达情况,探讨Bim表达水平与ALL患儿GC敏感性的关系.结果 地塞米松作用CEM-C7 后12 h Bim表达量开始增加,直到72 h仍维持在较高水平,c-Myc表达水平在地塞米松作用后24 h明显下降,到72 h降至最低,而在GC耐药细胞CEM-C1中 Bim和c-Myc表达水平均无变化.30例初诊ALL患儿,25例GC敏感,5例GC不敏感,Bim表达水平在GC敏感组GC诱导前及诱导后24 h均高于GC不敏感组,差异均有统计学意义(Pa<0.05),但在GC诱导后48 h、72 h及第8天,2组Bim表达水平比较差异均无统计学意义.GC敏感组诱导后不同时间点未发现Bim表达呈增高趋势.结论 GC通过上调Bim表达及抑制c-Myc的表达,而诱导ALL细胞凋亡;Bim有可能作为判断ALL患儿早期GC敏感与否的客观指标,从而指导临床治疗及判断预后.     

环索奈德干粉吸入剂的研究及其对豚鼠哮喘的抑制作用(英文)

环索奈德是目前处于临床研究阶段,用于治疗哮喘的新糖皮质激素类药物。本研究的目的是制备小剂量环索奈德干粉吸入剂(DPIs,80μg),并探讨其抗哮喘作用。采用球型乳糖作为稀释剂,与微粉化环索奈德混匀,制备环索奈德胶囊,置于干粉吸入装置中供口服吸入用。建立豚鼠哮喘模型,评价环索奈德抗哮喘作用。结果表明:制备得到的环索奈德干粉吸入剂的肺部沉积率约为26%,含量均匀度符合中国药典要求。在建立的哮喘豚鼠模型中,哮喘豚鼠肺泡间隔增宽,肺泡腔缩小,支气管黏膜上皮部分坏死、脱落,伴杯状细胞增生及嗜酸性粒细胞侵润。给予环索奈德治疗后,肺部病理症状得到了有效缓解或恢复,肺泡灌洗液(BALF)中细胞总数及嗜酸性粒细胞明显减少,IL-5水平降低,IFN-γ水平升高,表现出明显的治疗哮喘效果。本研究成功的制备了新型抗哮喘环索奈德肺部给药系统,并在动物模型上证实了其对哮喘的抑制作用。     

氟尿嘧啶关节腔内注射对兔膝关节的毒性作用研究

目的观察氟尿嘧啶（5 Fu）膝关节腔内注射对兔膝关节的毒性作用。方法将48只兔随机分成5 Fu 1 mg·kg 1组、5 Fu 2 mg·kg 1组、5 Fu 4 mg·kg 1组和对照组各12只，分别按1，2和4 mg·kg 1的剂量关节腔内注射5 Fu和等量0.9%氯化钠溶液，每周1次，连续4次。观察各组兔血液学参数、血液生化指标及肝、肾、膝关节内组织病理学变化。结果与对照组兔相比，5 Fu不同剂量组兔全身情况、血液学参数、血液生化指标均无明显改变；肝、肾未见明显病理学改变；关节局部结构软骨、滑膜交叉韧带、半月板亦未见明显病理性改变。结论对兔膝关节腔内注射5 Fu，剂量为1～4 mg·kg 1时局部及全身未见明显毒性作用。     

Lid margin split in the surgical correction of epiblepharon

Purpose: To describe the technique of splitting the lid margin combined with the excision of redundant skin and muscle during the surgical correction of epiblepharon and to report its clinical outcome. Methods: A combined procedure that included splitting the lid margin to repair lower eyelid epiblepharon was performed on 31 eyes of 19 consecutive patients. Lid margin splitting was performed along the grey line on the medial third or half of the lower eyelid by making a 1 mm‐deep incision. Having made a transverse subciliary skin incision and a dissection between the tarsus and the orbicularis oculi muscle, the subcutaneous tissue of the superior edge of the incision was secured to the tarsus with interrupted sutures to evert the cilia. An excision of the redundant skin and orbicularis tissue was made and the skin was closed. The patients were followed for direct inspection of the wound, the split lid margin, the direction of the lashes and the status of the cornea. Results: The mean postoperative follow‐up period was 29.4 weeks. Symptoms disappeared in all patients. In 30 eyelids of 19 patients the cilia did not touch the cornea, even in the down‐gaze. In one eyelid the cilium touched the medial conjunctiva, but not the cornea. The cosmetic outcome of the lower lid was satisfactory in all cases and the wounds of the split lid margin healed without scarring. To date, there have been no complications such as wound dehiscence, ectropion or eyelid retraction. Conclusions: The lamellar splitting of the lid margin is a beneficial addition to the repair of prominent lower lid epiblepharon, especially on the medial aspect of the eyelid. This simple technique ensures easier eversion of the cilia in epiblepharon repair, without disturbing the posterior lamella or causing unfavourable results.     

鼻咽癌放疗患者社交回避因素的描述性质性研究

目的 探讨鼻咽癌放疗患者社交回避的引发因素,为制订针对性护理策略提供依据。 方法 采用描述性质性研究方法,以目的抽样法选取12例鼻咽癌放疗患者为研究对象,对患者进行半结构式访谈,根据内容分析法对资料进行分析并提炼主题。 结果 提炼出3个主题7个亚主题,即理想自我形象构建无能（包括面对面交流困难、潜在的负面评价）,自我价值感削弱（现实的无效应对、社交活动参与度降低、加重家庭负担的愧疚感）,社会支持缺乏（亲友认同支持不足、专业支持不足）。 结论 医护人员需重视鼻咽癌患者社交回避的影响因素,加强医院、社区、家庭的协同合作,增强患者的自我价值感,减轻鼻咽癌放疗患者社交心理负担,使其重返社交环境。     

Biogenetic temperament and character and attention deficit hyperactivity disorder in Korean children

BACKGROUND: The objective of this study was to evaluate the relationship between symptoms of attention deficit hyperactivity disorder (ADHD) and biogenetic temperament, as assessed by the Junior Temperament and Character Inventory (JTCI) in Korean elementary school children. SAMPLING AND METHODS: Five hundred and sixteen elementary school students (254 boys and 262 girls; age range 9-14 years, mean age 11.0 +/- 1.0 years) were studied. The Junior Temperament and Character Inventory, both the self-rating and parent rating scales, the DuPaul ADHD rating scale (ARS-IV) for parents and teachers and the Child Behavior Checklist have been completed by study subjects. Children with high ARS-IV scores (the upper 10th percentile, n = 52) were defined to have ADHD. Fifty-two age- and sex-matched non-ADHD control subjects were selected randomly from a pool of 464 subjects. RESULTS: Inattention and hyperactivity/impulsivity symptoms correlated positively with Novelty Seeking in both children and parent ratings. These symptoms correlated negatively with Persistence, Self-Directedness and Cooperativeness. Novelty Seeking also had significant correlations with attention problems, delinquent behavior, aggressive behavior, externalizing problems and social problems in the Child Behavior Checklist. The ADHD group (n = 52) had higher scores of Novelty Seeking and lower scores of Self-Directedness and Cooperativeness, compared with the control group. CONCLUSIONS: There were significant correlations between ADHD symptoms and Novelty Seeking. Current findings suggest that Novelty Seeking and ADHD share biogenetic backgrounds.