HZ818树脂在紫杉醇初分离工艺中的应用

采用HZ818大孔吸附树脂对红豆杉浸膏中的紫杉醇成分进行吸附和洗脱试验，同时利用液质联用进行分析检测，确定了其最佳工艺参数。结果表明，HZ818树脂对紫杉醇有良好的吸附分离效果。优化工艺条件为：样品溶于体积分数40％甲醇，以1．5mL／min过柱，体积分数75％甲醇淋洗，体积分数85％甲醇以0．5mL／min洗脱，紫杉醇的质量分数从原浸膏中的1．02％提高到8．1％，回收率达98．6％。     

卫氏并殖吸虫成虫的特异性诊断抗原

目的：寻找诊断并殖吸虫病的特异性抗原。方法：应用SDS-PAGE和Western blot对卫氏并殖吸虫成虫可溶性抗原中的蛋白组分进行分析。结果：卫氏并殖吸虫成虫可溶性抗原蛋白经SDS-PAGE后显示14条蛋白带，其中相对分子质量为53000、3l000、25000、16000、11000是主带；相对分子质量为53000和34000的条带可与华支睾吸虫病患者血清反应；108000、94000、65000的条带可与血吸虫病患者血清反应；58000、53000、43000的条带可与旋毛虫感染的大鼠血清、小鼠血清及旋毛虫病患者血清反应。37000的蛋白带只能被斯氏狸殖吸虫感染的大鼠血清和并殖吸虫病患者血清所识别，而不与华支睾吸虫病、血吸虫病患者血清、旋毛虫感染的大鼠和小鼠血清、旋毛虫病患者血清、正常大鼠、小鼠血清及正常人血清发生交叉反应。结论：卫氏并殖吸虫成虫可溶性抗原中相对分子质量为37000的蛋白组分为卫氏并殖吸虫成虫的特异性抗原，可用于并殖吸虫病的免疫学诊断及血清流行病学调查．     

SPM5软件分析癫痫患者脑PET图像的应用研究

Objective To investigate the value of statistical parametric mapping 5 (SPM5) and its parameter settings in analysis of PET imaging for epilepsy patients. Methods Seventeen epilepsy patients and seventeen controls were scanned with PET. The datns were analyzed using PET and SPM5 with qualitative and semiquantitative analysis, statistical analysis of the percentage (numbers of agent distribution decreasing in focus to brain) for different parameters combinations, which were value adjustment to contral-p and voxels-k. Results There was decreasing of agent distribution at temporal lobe in epilepsy patients detecting with PET; the T/NT of focus to cerebellum was 1.07±0.24, the T/NT of normal tissue in opposite side to cerebellum was 1.27±0.18, there was a significant difference (t=1.87, P<0.05). Dealing with SPM5, there was more significant decreasing of agent in regions 19, 21, 39 of temporal lobe, regions 7, 19, 31, 40, 47 of parietal lobe and occipital lobe in epilepsy patients. With different value of k, the percentage was (27±22)%, (29±24)%, (32±23)%, (35±27)%, (39±31)%respectively, there was not significant difference (F=0.59, P>0.05) ; for different value of p, the percentage was (42±30) %, (29±25) %, (26±21) % respectively, with a significant difference (F=3.60, P<0.05); there was linear regression in value adjustment to contral-p and the percentage(b=-18.24, t=2.57, P<0.05). Conclusions Semiquantitative analysis by SPM5, the setting of value adjustment to contral-p would affect the results, the smaller of value adjustment to contral-p, the better of the result. SPM5 would be more objective and accurate to locate the focuses.     

重症急性胰腺炎大鼠肺组织中LIF的表达变化及意义

目的:观察重症急性胰腺炎(SAP)大鼠白血病抑制因子(LIF)在肺组织中表达的时相变化, 探讨LIF在SAP病程及肺损伤中的意义．方法:36只♂SD大鼠随机分为正常对照组(N 组,n=6)、假手术组(Sham组,n=6)和重症急性胰腺炎组(SAP组,n=24)．采用胰管逆行灌注50 g/L牛磺胆酸钠的方法复制大鼠SAP模型．用RT-PCR法检测肺组织中LIF mRNA的表达水平,免疫组织化学方法检测NLIF在肺组织中的表达变化．结果:SAP组3 h后肺组织LIF mRNA的表达量明显高于对照组和假手术组(灰度值:1．018± 0．065 vs 1．451±0．067,1．322±0．072,P<0,05), 并且6,12,24 h持续升高(0．853±0．058,0．635 ±0．064,0．582±0．089)(P<0．01)．同样,SAP组 LIF蛋白表达在3和6 h后明显高于对照组和假手术(127．36±2．76,122．53±2．43 vs 159．46 ±2．78,156．35±3．12,P<0．05),并且12,24 h后也维持在很高的水平(109．37±2．87,102．42± 2．27)．结论:LIF作为促炎症因子参与了SAP肺组织的炎症反应．     

脂肪乳剂在重症急性胰腺炎营养治疗中的安全性及有效性分析

目的 :探讨脂肪乳剂应用于重症急性胰腺炎的安全性和有效性。　方法 :回顾分析重症急性胰腺炎病人肠外营养治疗期间 ,应用脂肪乳剂的影响。 74例重症急性胰腺炎病人行肠外营养治疗 ,按静脉营养液是否含脂肪乳剂分为脂乳组和非脂乳组。　结果 :脂乳组肠外营养治疗后清蛋白、前白蛋白改善显著 (P <0 .0 1 ) ,非脂乳组则无显著变化 (P >0 .0 5 ) ,脂乳组较非脂乳组改善明显 (P <0 .0 1 ) ;血清肌酐、尿素氮等在脂乳组治疗后下降明显 (P <0 .0 5 ) ,较非脂乳组改善显著 (P <0 .0 5 ) ;营养治疗结束后 ,脂乳组高血糖症状明显得到控制 (P <0 .0 1 ) ,而非脂乳组效果不佳 (P >0 .0 5 ) ,脂乳组优于非脂乳组 (P <0 .0 5 ) ;血淀粉酶均有下降 ,脂乳组未见异常增高。　结论 :重症急性胰腺炎病人输注脂肪乳剂安全有效 ,有助于恢复正氮平衡     

脾脏切除对胸腺和淋巴结免疫功能的影响

报道脾脏切除（脾切）对动物胸腺和淋巴结免疫功能的影响，脾切后３ｗ大鼠胸腺细胞自发增殖及ＣｏｎＡ诱导增殖均无明显变化，ＣｏｎＡ诱导淋巴结细胞增殖脾切组明显高于假手术对照组，脾切后３ｗ再用ＳＲＢＣ免疫，５ｄ后小鼠腹腔淋巴结数量脾切明显高于对照组，用ＱＨＳ法测定淋巴结细胞对ＳＲＢＣ初次抗体产生脾切组则明显低于对照组。     

五型病毒性肝炎的暴露因素与临床表现的比较

比较急性病毒性肝炎患者感染打危险因素，同时比较五型肝炎的症状和体征。连续收集急性病毒性肝炎患者血清２９３份，用ＥＬＩＳＡ法检测了五型肝炎病毒的病原学指标，并进行了流赞美同学调查。暴露因素：丙肝的肠道外暴露外明显多于甲肝；甲肝和戊肝有较高的“甲肝”患者接触史；乙肝，丙肝和丁肝有高的“乙肝”接触史，与甲肝，戊肝相比差异显著。     

Clinical observation of Qi deficiency syndrome in 72 patients with chronic obstructive pulmonary disease treated with Yiqi Mianyi Granule

Seventy-two patients of chronic obstructive pulmonary disease (COPD) of Qi deficiency syndrome with abnormal immune indices were treated with Yiqi Mianyi Granule (YQMYG) and the efficacy was compared with that of 30 cases treated with Zhenqi fuzheng Granule (ZQFZG) for control. Results showed that the markedly effective rate of symptomatic improvement of Qi deficiency in YQMYG group was 65.3%, the total effective rate 93.1%. 88.6% of the immune indices lower than normal were corrected and 43. 7% of them were normalized, while for indices that were higher than normal the rate were 78.2% and 52.9% respectively. These results suggested that YQMYG could improve symptom of Qi Deficiency markedly, strengthen cellular immunity and regulate immune dysfunction. Its therapeutic efficacy was obviously superior to ZQFZG (P<0.Q5).     

人左、右心室阻抗微分心动图的比较研究

作者利用Ⅱ导心电图、心音图、颈动脉搏动图、右（或左）心室阻抗微分心动图同步描记的方法。对人左、右心室阻抗微分心动图进行了比较分析。结果显示:人的左、右心室阻抗微分心动图图形相似，波形稳定，转折明显，均有清晰的生理标志点。两种图形各相应生理标志点在时相上存在着区别．且人的左、右心室阻抗微分心动图能分别反映人的左、右心室收缩和舒张过程，说明利用人的左、右心室阻抗微分心动图能分别测定人的左、右心室收缩时间间期与舒张时间间期值，鉴别和评定左、右心室的功能。     

家兔实验性减压病时血浆中血拴素A_2及前列环素含量的变化

实验观察了“安全”减压和不适当减压条件下家兔减压病(DCS)的发病情况、Doppler超声以及血浆中TxB_2和6-keto-PGF_(1a)的变化;还观察了消炎痛对DCS的预防怍用。结果显示:减压愈不当,DCS发病愈重,Doppler超声气泡探测仪检测到的级别愈高。血浆TxB_2、6-keto-PGF_(1α)值在濒死动物中明显升高(P<0.01);存活动物中,TxB_2经历了下降、再恢复的过程(P<0.01),而6-keto-PGF_(1α)值未见明显变化。消炎痛在抑制血浆TxB_2升高的同时,有效地降低了DCS发病率。此结果表明:TxA_2、PGI_2参与了重型DCS的发病过程,消炎痛的顶防作用与抑制花生四烯酸代谢物的生成有关。