爱西特对小分子尿毒素的吸附作用及其影响因素

目的：研究爱西特对小分子尿毒素的吸附能力及影响其吸附的因素。方法：将尿素、肌酐、尿酸标准品加入蒸馏水配成尿毒素溶液并加入爱西特作用不同时间，检测其尿毒素成份。干扰组分别加胆汁、氨基酸、林格氏液、葡萄糖液、Hcl和NaOH，并同时加入爱西特吸附后检测尿毒素浓度变化。结果：尿素、肌酐、尿酸浓度5min开始下降，尿素40min达最低值，50min后有反弹，肌酐、尿酸10～30min达最低值，以后趋稳定。胆汁、氨基酸、电解质、葡萄糖液、酸碱等对爱西特吸附尿酸无明显影响，但对尿素、肌酐吸附有不同程度减弱作用，胆汁、氨基酸对尿素干扰最大，葡萄糖液、酸碱次之；pH2．0液对肌酐吸附干扰最大，葡萄糖次之。结论：爱西特对尿素、肌酐、尿酸有良好的吸附能力．但随著吸附时间的延长．吸附能力下降。胆汁、含氮物、糖及不同DH对爱西特的吸附有不同程度的影响。     

储存血小板源细胞外囊泡中非编码RNA表达谱及其潜在临床意义

目的 通过对不同储存期血小板源细胞外囊泡(P-EV)的转录组学分析,进而研究胞外囊泡在血小板质量评价及输血治疗中的潜在作用.方法 以二代测序检测P-EV的全转录组,利用生物信息分析预测非编码基因的靶标及功能,实时PCR(RT-PCR)验证表达趋势.结果 通过对不同储存期P-EV变化的非编码RNA(ncRNA)进行测序和...     

开窗腰椎间盘切除再手术的远期疗效

目的探讨开窗腰椎间盘切除再手术治疗初次开窗髓核摘除术失败患者的远期疗效和影响因素。方法回顾性研究51例行第二次开窗腰椎间盘切除术的患者临床资料,随访时间120~203个月,平均随访146.8个月。按MacNab分类和JOA评分标准评价满意度和远期功能。对放射学资料完整的患者进行手术前后椎间隙高度对比研究。结果51例患者中,结果优良率70.6%,失败率15.7%,患者总体满意率78.4%。除了8例失败患者,远期随访的43例患者JOA评分平均提高(64.6±18.2)%,术后行走能力明显改善。对手术节段椎间隙高度测量发现术后椎间隙高度明显下降,但不影响远期功能结果。吸烟、是否有外伤等发病诱因、合并纤维化和初次术后症状未缓解期对远期结果有影响。心理社会因素与预后可能存在一定关系。结论开窗腰椎间盘切除再手术治疗初次开窗髓核摘除术失败患者疗效满意,但手术复杂性高,明确术前诊断和严格手术指征非常重要。JOA评分被证明为易于患者和医师实施,量化主观数据的评估法。     

Construction of AP003469.4-miRNAs-mRNAs ceRNA network to reveal potential biomarkers for hepatocellular carcinoma

Studies have reported that the competing endogenous RNA (ceRNA) networks are related to disease progression and prognosis in patients with hepatocellular carcinoma (HCC). The roles and mechanisms of long-chain non-coding RNA AP003469.4 in HCC have remained unclear. Here, we explored the roles of AP003469.4 in HCC progression using bioinformatics, CCK-8, Transwell assay, etc. AP003469.4 targets miRNAs and these target genes were predicted by the LncBase Predicted v.2, miRDB, miRTarBase, and TargetScan databases. Then, AP003469.4-associated ceRNA network was constructed. Biological functions and mechanisms of differentially expressed genes in the ceRNA network were explored using GO and KEGG. Survival analysis and Cox regression analysis were used to screen prognostic genes and construct a prognostic risk model. The results revealed that AP003469.4, with the area under the curve of 0.9048, was highly expressed in HCC tissues. Increased expression of AP003469.4 was an independent risk factor for the dismal prognosis of HCC patients and was associated with the short overall and disease-free survival. Downregulation of AP003469.4 expression inhibited cell proliferation, cycle transition, invasion, and migration, and promoted cell apoptosis. There were 489 differentially expressed target genes in the ceRNA network, which were involved in several pathways, such as the MAPK signaling pathway, cell cycle, and p53 signaling pathway. The risk model was based on the DTYMK, ZFC3H1, CBX2, PKM, TTC26, ATG10, TAGLN2, CD3EAP, SHISA9, SLC1A5, KPNA2, SCML2, E2F7, and SMARCD1, which were the independent risk factors for poor prognosis of HCC patients. In general, interference with AP003469.4 expression might delay the progression of HCC. AP003469.4 related network could help to identify the hub target molecules in HCC progression, which might be candidate biomarkers for evaluating the prognosis of HCC patients.     

Endoscopic features of esophageal high-grade intraepithelial neoplasia dominated by cytological atypia

Little is known about esophageal high-grade intraepithelial neoplasia dominated by cytological atypia (HGINc). We aimed to elucidate the endoscopic features of HGINc compared with esophageal high-grade intraepithelial neoplasia dominated by architectural atypia (HGINa). All patients pathologically diagnosed as esophageal high-grade intraepithelial neoplasia after endoscopic submucosal dissection at our center between January 2018 and December 2019 were included in this study. According to the pathological diagnosis, the patients were divided into two groups: HGINa group and HGINc group. Basic characteristics and endoscopic information were collected in detail. Data were analyzed statistically. Binary logistic regression was performed and a predictive model for HGINc was established. Then we evaluated its predictive value and built a nomogram for clinical application. A total of 175 patients were included in this study (126 with HGINa and 49 with HGINc). Among 228 lesions found in all patients, there were 148 HGINa and 80 HGINc. The independent relevant factors for HGINc were tobacco and alcohol usage, color, and gross type. To predict risk of HGINc, a three-factor model (TFM) was established with a highest area under curve (AUC) as 0.869 (95% CI, 0.852, 0.939). When the cut-off value was set as 0.3569184, the diagnostic accuracy, sensitivity, specificity, positive predictive value, and negative predictive value for HGINc was 81.14%, 88.75%, 77.03%, 67.62%, and 92.68%, respectively. HGINc differs greatly in endoscopic features from HGINa in our study. It’s important to reduce misdiagnosis that our model was established with good predictive value for clinical application.     

早期切痂植皮治疗手背Ⅲ度烧伤44例总结

目的探讨早期切痂大张中厚自体皮移植治疗手背Ⅲ度烧伤的效果。方法对44例57只手背Ⅲ度烧伤患者,实施早期切痂大张中厚自体皮移植。皮肤成活拆线后,即行主、被动功能练习并借助各种器械辅助练习,同时配合药物、按摩、弹力压迫、浅部X线照射等综合康复治疗半年以上。结果本组患者全部治愈,经半年以上随访,功能与外观达优者42只手,良12只手,差3只手。结论早期切痂大张中厚自体皮移植及术后功能练习配合综合康复疗法,治疗手背Ⅲ度烧伤,效果好,功能恢复理想。     

肝动脉栓塞加手术切除治疗大肝癌37例

作者报告３７例大肝癌采用肝动脉栓塞（ＴＡＥ）加手术切除的疗效及临床病理研究结果。３７例肝癌直径５～２４ｃｍ（平均１１．２Ｃｍ）。ＴＡＥ与动脉灌注化疗同时进行。化疗药物括氟尿嘧啶（５－ＦＵ）、阿霉素（ＡＤＭ）或表阿霉素（Ｅ－ＡＤＭ）、丝裂霉素（ＭＭＣ）和顺铂（ＣＤＤＰ）。多采用三种药物联合方案。肝动脉末梢栓塞剂采用国产或进口碘化油,用明胶海绵颗粒作近端栓塞。手术切除前进行１～４次ＴＡＥ,每次相隔４～６周。１７例ＡＦＰ值增高者ＴＡＥ后１０例降至正常水平。肿瘤直径由平均１１．２ｃｍ降至８．５ｃｍ（缩小２６％）。栓塞后手术切除病理标本显示９２％有肿瘤组织坏死,范围达４０％～１００％。１、２、３年生存率分别为８０％、６６．７％和５３．３％。作者认为ＴＡＥ加手术切除是大肝癌的有效治疗方法。     

血液流变学特性改变在＋Gz致大鼠脑损害中的意义

目的：探讨＋Ｇｚ重复作用后大鼠血液流变学特性的变化规律及其在＋Ｇｚ致脑损害中的作用．方法：利用动物离心机将大鼠重复暴露于＋１０Ｇｚ３ｍｉｎ三次，观察暴露后不同时间血液流变学特性及脑组织形态学的变化．结果：＋Ｇｚ重复暴露３次后即刻及１ｈ，大鼠全血粘度、全血还原粘度、血浆纤维蛋白原含量、红细胞聚集指数及屈服应力较对照组均显著升高（Ｐ＜０．０５）；暴露后６ｈ，全血粘度、全血还原粘度及血浆纤维蛋白原含量已基本恢复至对照组水平，红细胞聚集指数及屈服应力也呈现出恢复趋势，但仍明显高于对照组（Ｐ＜０．０５）．＋Ｇｚ暴露后各时间组的血浆粘度、红细胞体积分数、红细胞刚性指数及Ｃａｓｓｏｎ粘度则均无明显变化．＋Ｇｚ重复暴露后大鼠脑皮层少数神经元出现缺血性改变．结论：＋１０Ｇｚ３ｍｉｎ重复暴露３次可引起大鼠血液流变学特性的改变．全血粘度的增高可能主要与红细胞聚集程度增加有关．血液流变学特性的改变可能对加重＋Ｇｚ重复暴露引起的脑损害有一定影响．