猪全肠外营养模型

白色杂种猪 19只 ,体重 2 2 .3± 2 .4 kg,经皮颈总静脉穿刺前腔静脉置管 ,其中 14只动物完成 2 8d全肠外营养 ( TPN)。 14只动物 ,共施行置管 2 9次 (平均 1.31± 0 .86次 ) ,成功率为10 0 %。再次置管的原因为导管脱出 ( 7/ 2 9)和导管栓塞 ( 2 / 2 9) ,本研究还对与腔静脉置管相关静脉进行了解剖     

铅性肾病的流行病学分析

我们检查了铅接触人员１０２３名，发现铅性肾病８４例，患病率８．２％。女性３４例（７．２％），男性５０例（９％），男女铅性肾病患病率无显著性差异。印刷厂空气铅浓度平均０．００６９ｍｇ／ｍ３，蓄电池厂为０．１００５ｍｇ／ｍ３，铅矿为０．５ｍｇ／ｍ３。铅性肾病患病率分别为４．７３％，８．３％和２８．５７％（Ｐ＜０．００５）。分析铅性肾病患病率与年龄和铅接触年限的关系。年龄小于３０岁，～４０岁，～５０岁和大于５０岁者，铅性肾病患病率分别为５．５％，８．６％，１２．１％，和１５．６％（Ｐ＜０．０５）。铅接触年限小于１０年，～２０年和大于２０年者，铅性肾病患病率分别为６．６％，９．９％和１３．１％（Ｐ＜０．０５）。     

自血光量子疗法治疗高粘滞血症30例疗效观察

采用自血光量子疗法治疗高粘滞血症３０例，男２１例，女９例。其中伴发冠心病者７例，高血压病６例，肺心病６例，脑血栓５例，糖尿病４例，脑出血，多发性肺囊肿、药物性皮疹、肺炎各１例，单纯高粘滞血症８例。另设健康对照组３０例。经一疗程治疗，比较患病组治疗前后全血粘度、红细胞聚集指数及红细胞压积均明显降低。本文结果表明自血光量子疗法对高粘滞血症有明显疗效，     

Monitoring of serum markers for fibrosis during CCl4-induced liver damage. Effects of anti-fibrotic agents.

Liver fibrosis was induced in rats by repeated peritoneal injections of carbon tetrachloride (CCl4) over a period of 2-11 weeks. Serum procollagen III peptide (SPIIINP), prolidase (SP) and alanine aminotransferase (SALT) levels were monitored during the period of induction. The extent of fibrosis was semi-quantitatively estimated after collagen staining, and the anti-fibrotic effects of 16,16-dimethyl prostaglandin E2 (DMPGE2), colchicine, and zinc sulphate were studied. SPIIINP and SP were increased the first 2 weeks after CCl4 administration and peaked at 6 weeks. Alterations in SPIIINP and SP correlated well to the semi-quantitative histological score of liver sections during the first 6 weeks, and SP was positively related to SPIIINP throughout the whole induction period. DMPGE2 decreased SPIIINP, SP and SALT significantly in addition to a markedly decreased formation of liver collagens. Colchicine had a similar but less dramatic effect, whereas zinc sulphate only reduced SPIIINP without influencing liver damage. In conclusion SPIIINP seems to be a valuable indicator of liver fibrogenesis, and SP may play a limited role in indicating accelerated collagen metabolism in the liver. DMPGE2 obviously inhibited the production of collagens induced by CCl4. Colchicine also had an apparent effect on liver fibrosis, whereas zinc sulphate merely seemed to postpone it.     

大黄对实验性急性化脓性胆管炎早期血浆内毒素含量的影响

取ＳＤ大鼠，随机分为三组：正常组、盐水组、大黄组。利用大肠杆菌诱发急性化脓性胆管炎，盐水组和大黄组分别经口灌服生理盐水和大黄１ｍｌ／１００ｇ·日。以血浆内毒素及血清酶学为指标，观察了大黄对大鼠急性化脓性胆管炎早期血浆内毒素及肝功的影响。结果显示：１．盐水组术后３天内死亡４只，大黄组死亡２只，正常组无死亡。２．盐水组大鼠术后３天血浆内毒素含量明显升高，大黄组含量较盐水组明显降低（Ｐ＜０．０１）。３．术后３天血清ＡＫＰ和ＧＰＴ与正常组比均有明显增高，而盐水和大黄组之间无显著性差异（Ｐ＞０．０５）。结果表明大黄具有降低化脓性胆管炎大鼠的早期死亡率及显著降低内毒素血症的作用。     

酶学,B型超声,CT及ERCP检查对慢性胰腺炎与胰腺癌的鉴别诊断价值

对经手术和/或病理证实的130例慢性胰腺炎和101例胰腺癌的酶学、B型超声、CT及ERCP检查对比分析。结果表明胰腺癌组ALP和γ-GT明显高于慢性胰腺炎组（ALP84.9％与32.1％，γ-GT93.8％与57.l％，p＜0.01）。B型超声对慢性胰腺炎与胰腺癌的诊断正确率分别为65.7％与72.9％（p＞0.05），CT分别为64.0％与80.0％（p＞0.05），ERCP分别为37.3％与72.7％（p＜0.01）。结果提示ALP/γ-GT与ERCP对慢性胰腺炎与膜腺癌有鉴别诊断意义。     

实验性骨折愈合中骨吸收活动的超微结构观察

将50只家兔造成实验性桡骨骨折,分批取骨痂标本,用光镜和电子显微镜观察表明,在骨折愈合过程中,破骨细胞是骨吸收的主要执行者,巨噬细胞能吞噬死骨,但不能吸收骨质。     

质粒pGH/BL_(21)在大肠杆菌中表达条件的研究

目的　寻找质粒 pGH/BL2 1在大肠杆菌中的最佳表达条件。方法　当它在大肠杆菌中表达时 ,考查不同IPTG诱导浓度及不同的诱导时间对表达产物的影响 ,表达产物的量以SDS -PAGE电泳方法分析。结果　该基因的最佳表达条件为 :IPTG诱导浓度 1.0mmol/L ,诱导时间 4h。结论　IPTG对不同的基因在不同的表达体系中需不同的诱导条件 ,只有找到它的最佳诱导条件才能保证终产物的最大产率     

REGULATING FUNCTION OF COENZYMIZATION AND DECOENZYMIZATION OF THE LACTATE DEHYDROGENASE ISOZYMES IN THE MOUSE TISSUES DURING HYPOXIA

Objective. To study the characteristics of changes of LDH enzyme patterns of mice under slight hypoxia.Methods. Mice treated with artificial hypoxia, various tissues were made for the test of LDH enzymatic activity by the specific staining technique. LDH (1 -5) relative percentage enzymatic activity (RPEA) were measured with CS-910 dual-wavelength thin layer chromatography scanner.Results. The RPEA of LDH isozymes of various tissues after slight hypoxia shifted to the isozymes LDH1 and LDH2, whose principal subunits are H subunits, and the RPEA of LDH,(H4), LDH2(H3M) increased, while RPEA of LDH5(M4) in various tissues decreased prominently except the cardiac muscle, and that of LDH4(HM3) decreased as well. After polyacrylamide gel electrophoresis (PAGE) of the hypoxia treated cardiac muscle specimen was made, activity subbands originated regularly in the isozyme patterns of LDH, with the regularity of LDH1 (0 subband), LDH2 (0-1 subbands), LDH3 (0-2 subbands), LDH4 (1-3 subbands), LDH5 (2-4 subbands).