Embryo developmental stage at transfer influences outcome of treatment with intracytoplasmic sperm injection

Purpose: Our purpose was to determine if embryo cell stage at the time of intrauterine transfer correlates with pregnancy rate in patients treated with intracytoplasmic sperm injection (ICSI). Methods: We conducted a retrospective analysis of 455 embryo transfer cycles following ICSI and 304 conventional in vitro fertilization (IVF) and embryo transfer cycles in women aged 40 years or less. Abstracted information included grading of the embryo cell stage and quality at the time of transfer. Results: The overall ICSI pregnancy rate was 30.8%, while that of conventional IVF was 29.3%. However, the ICSI pregnancy rate fell to 9.3% for embryo transfers taking place at the two-cell stage but increased to 35.8% when at least one embryo had more than two cells, and this difference was statistically significant (P≤0.0001). The pregnancy rate following conventional IVF was 22.0% when only two-cell embryos were transferred and 32.0% when at least one of the embryos had more than two cells, but this difference in pregnancy rates was not significant (P>0.05). Conclusions: The stage of embryo development at transfer appears to exert a powerful influence on the successful establishment of pregnancy after ICSI.     

双重法固定肩锁关节脱位

为介绍一种新方法修复肩锁关节脱位。肩锁关节脱位整复后采用双重法（交叉克氏针及8字钢丝）固定肩锁关节。结果：16例肩锁关节脱位后无一复发,关节功能无影响。结论：该法操作简单,固定牢靠,可早期行关节功能锻炼,可有效防止脱位复发。     

The role of MRI in the illustration of metastatic lymphatic pathways and clinical N-staging of nasopharyngeal carcinoma

Subject: To study the role of MRI in the illustration of metastatic lymphatic pathways and clinical N-staging of nasopharyngeal carcinoma (NPC). Methods: Eighty NPC patients were examined with MRI before radiotherapy from Mar. 1994 to Jun. 1996. MRI were performed using T₁ weighted image (T,WI) and T₂ weighted image (T₂WI) in transverse, and using T₁WI in sagittal and coronal sections. Results: 1. NPC chief metastatic lymphatic pathways are: primary foci → Rouviere’s node (RN), or retrostyloid space nodes (RSN) secondarily → deep cervical nodes; 2. The superior border of neck fields should be moved upward to the level of external acoustic meatus; 3. The authors suggested that in N-staging for NPC, N_o and N₁ be divided into Noa and N₁b, and N₁a and N₁b. Conclusion: MRI is very useful in clinical N-staging of NPC, especially for the reflection of the influance of RN and/or RSN tumefaction on N-staging.     

乙酰肝素酶mRNA在大肠癌中的表达及意义

目的:探讨乙酰肝素酶在大肠癌中的表达及意义.方法:应用原位杂交方法检测乙酰肝素酶mRNA在45例大肠癌组织中的定位及表达.结果:45例大肠癌组织中,乙酰肝素酶mRNA阳性表达22例(48.88%).26例大肠癌伴淋巴结转移者,其原发灶内乙酰肝素酶mRNA表达明显高于无转移组,有显著差异(P＜0.01).乙酰肝素酶mRNA表达与大肠癌组织浸润深度、淋巴结转移有关.结论:乙酰肝素酶可能在大肠癌的生长、浸润和转移中起一定的作用.     

稳态强噪声暴露后听神经动作电位及神经丝蛋白表达的观察

目的 观察豚鼠暴露于稳态强噪声后听神经复合动作电位(CAP)及神经丝蛋白(NFP)的变化。方法 选用20只(40耳)Preyer反射正常的健康豚鼠，分为4组，噪声暴露后存活7天组(N-7d)、14天组(N-14d)和2个月组(N-2m)各5只，空白对照组(C)5只。噪声强度130dB(A)SPL，连续暴露5小时，于暴露前、暴露后3、7、14天、暴露后2个月分别测试CAP—N1波反应阈。免疫组化反应分别检测对照组、暴露后7、14天组、暴露后2个月组动物耳蜗内NFP的表达。结果稳态强噪声暴露后CAP—N1波反应阈明显增高，于暴露后7天基本稳定。暴露后7、14天NFP表达无明显变化，暴露后2月NFP表达显著减弱。结论 130dB(A)SPL稳态强噪声暴露5小时可以导致豚鼠CAP中重度永久性阈移，噪声损伤的早期NFP改变不明显，耳蜗NFP染色反应随着存活时间延长逐渐减弱，反映了损伤后神经纤维中骨架蛋白存在变性过程。     

短暂性脑缺血发作对后继脑梗死影响的研究

目的:评价短暂性脑缺血发作(T IA)对后继脑梗死是否有保护作用。方法:选择2000~2005年我院老年神经病科及神经内科既往有同侧T IA的脑梗死患者98例,并对有同侧T IA的脑梗死患者根据T IA发作持续时间分为<10m in(26例)、10~20m in(38例)及>20m in(34例),并选择同期住院无T IA的脑梗死患者102例进行病例对照观察。在入院及病程一月时采用改良的爱丁堡-斯堪的那维亚卒中量表进行神经功能缺损评分,病程一月时进行预后评分。结果:两组患者年龄及其他危险因素包括高血压病、糖尿病、高脂血症及冠心病无差异(P>0.05);入院及病程一月时,无T IA的脑梗死患者其神经功能缺损评分及近期预后与T IA持续时间在10~20m in的脑梗死患者有显著性差异(P<0.05),但与T IA持续时间<10m in及>20m in的脑梗死患者无差异(P>0.05),且T IA持续时间在10~20m in之间与<10m in、>20m in比较有显著性差异(P<0.05)。结论:T IA对后继脑梗死有保护作用。     

医患谈话制度是医患之间绿色的桥梁

为了有效地保护患者的合法权益,加强医患之间的沟通与理解,督促医务人员改善服务态度,提高医疗质量,防范医疗纠纷的发生,我院从2002年4月起,在全院所有病区推行了医患谈话制度.     

睾丸扭转8例临床报道

睾丸扭转临床少见，是青少年急性阴囊疼痛的主要原因，常导致睾丸缺血坏死，早期诊断和及时手术是治疗的关键。我院自1998年1月～2004年12月共诊治8例，现报告如下。     

络刺临床应用举隅

《素问·调经论》曰:“络刺者,刺小络之脉也”。当今,络刺临床应用有所拓宽,已把各种浅刺放血法,如三棱针、皮肤针重刺出血法均归属于络刺范围。络刺具有活血化瘀、通络止痛、泻火解毒、启闭开窍之功。络刺是针灸临床常用方法之一,属九刺之一,具有操作简便、疗效显著的优点,若辨证准确,常可收到立竿见影的效果。现举数例如下。软组织挫伤王某,女,42岁。因肩周炎来我科治疗,每次治疗完毕后,嘱其每天多作肩部活动。由于本人治病心切,活动时用力过大,导致左肩胛部肌肉损伤,左侧肩部活动受限。以三棱针点刺患处3~5针,放血2~3ml,并拔罐治疗。取罐…     

泛素水解酶22调控丝裂原活化蛋白激酶激酶6基因的转录活性

目的：克隆丝裂原活化蛋白激酶激酶6(mitogen-activated protein kinase kinase 6,MKK6)基因启动子,研究泛 素水解酶22(ubiquitin specific peptidase 22,USP22)对MKK6转录活性的调控作用。方法：采用PCR扩增MKK6基因启动 子片段,并以该片段为模板对USP22结合位点进行定点突变,将野生型与突变型启动子片段定向插入荧光素酶表达 载体pGL3-Basic;用重组载体与内参质粒pRL-TK共转染HeLa细胞,行双荧光素酶活性检测以确定其转录活性;利用 染色质免疫共沉淀(chromatin immunoprecipitation,ChIP)实验观察USP22蛋白与MKK6启动子是否存在直接的结合;下 调USP22表达后,检测MKK6转录活性的变化。结果：成功扩增MKK6启动子及其突变体并构建荧光素酶表达载体; USP22结合位点的突变导致该启动子活性在HeLa细胞中明显降低(P<0.05);USP22与MKK6启动子在细胞中存在直接结 合;抑制USP22的表达导致MKK6转录水平明显下降(P<0.05)。结论：在HeLa细胞中,USP22有效地调控MKK6基因的 转录。