异常凝血酶原（PIVKA-Ⅱ）对TACE治疗肝癌疗效预测

【摘要】　目的?探索异常凝血酶原（PIVKA-Ⅱ）在接受TACE的肝癌患者血清中的表达及其临床意义。方法?自2013年1月至2016年1月,接受TACE治疗的肝癌患者170 例纳入本研究,收集TACE术前及术后1个月血清标本。检测血清中PIVKA-Ⅱ和AFP的表达水平,按照实体肿瘤应答评估标准（RECIST）评估抗肿瘤治疗反应­­。根据血清AFP和PIVKA-Ⅱ水平在1个月时与术前基础值相比的变化情况,以介入术后1个月随访腹部增强MRI观察肿瘤大小为标准,分析其与肝癌介入术后治疗反应的关系。结果?患者AFP在介入治疗术前、术后的变化与治疗反应无明显相关性;介入术前、术后PIVKA-Ⅱ的下降情况与治疗反应正相关;PIVKA-Ⅱ和AFP联合观察,与肿瘤的介入治疗反应相关性更明显。且PIVKA-Ⅱ和AFP两种肿瘤指标无明显相关性。结论?PIVKA-Ⅱ水平的降低是肝癌介入栓塞治疗效果和预后判断的良好指标,且与AFP联合判断,效果更确切。     

Screening of novel epilepsy-related genes and isolation and identification of cDNAs

Epilepsy is a brain dysfunction syndrome characterized by excessive. disorderly discharge ofcerebral neurons. The cause and mechanism ofepilepsy has not been established. Since epilepsy iscaused by multiple factors working together andpossesses heterogeneity, it is necessary to searchfor epilepsy--related genes (ERG). We isolated andcloned several novel epilepsy--related gene fragments by using mRNA differential display technique (mDD), which established a new way to Investigate the molecu…     

The Effect of Coriaria Lactone on NMDA Receptor Mediated Currents in Rat Hippocampal CAI Neurons

To investigate the exact mechanism of epileptogenesis induced by coriaria lactone (CL), the effect of CL on NMDA receptor mediated current (Iasp) in rat hippocampal CAI neurons was investigated by using nystatin perforated whole-cell patch clamp. 10-6-10-4 mol/L Asp acted on NMDA receptors and elicited an inward current (Iasp) at a holding potential (VH) of -40mV in presence of 10-6 mol/L glycine and absence of Mg2+ extracellularly. CL enhanced NMDA receptor mediated current induced by Asp, but had no effect on threshold concentration, EC50,Hill coefficient as well as maximal-effect concentration and reversal potential of Iasp. The effect had no relationship with holding potential. These results showed that CL could enhance NMDA receptor mediated current to increase [Ca2+]I of neurons by acting on Gly site, thereby inducing epilepsy.     

Intelligent Design of Construction Materials: A Comparative Study of AI Approaches for Predicting the Strength of Concrete with Blast Furnace Slag

Concrete production by replacing cement with green materials has been conducted in recent years considering the strategy of sustainable development. This study researched the topic of compressive strength regarding one type of green concrete containing blast furnace slag. Although some researchers have proposed using machine learning models to predict the compressive strength of concrete, few researchers have compared the prediction accuracy of different machine learning models on the compressive strength of concrete. Firstly, the hyperparameters of BP neural network (BPNN), support vector machine (SVM), decision tree (DT), random forest (RF), K-nearest neighbor algorithm (KNN), logistic regression (LR), and multiple linear regression (MLR) are tuned by the beetle antennae search algorithm (BAS). Then, the prediction effects of the above seven machine learning models on the compressive strength of concrete are evaluated and compared. The comparison results show that KNN has higher R values and lower RSME values both in the training set and test set; that is, KNN is the best model for predicting the compressive strength of concrete among the seven machine learning models mentioned above.     

聚桂醇在食管裂孔疝治疗中的应用研究

目的探讨聚桂醇在食管裂孔疝(HH)治疗中的疗效。方法选取HH患者69例,常规进镜至胃腔,退镜至食管膈裂孔压迹处,通过内镜活检孔插入注射针,在食管膈裂孔压迹处、齿状线处及LES区环周分别多点注射聚桂醇,点间隔1 cm,总量小于10 ml,每周一次,4次为一个疗程。疗程结束后1个月再次测定食管pH值、食管压力并胃镜检查,比较注药前后患者的食管pH值、食管压力指数、内镜下黏膜改变及临床症状改善情况。结果注药后1个月的食管pH值、食管压力及齿状线与膈食管裂孔压迹的距离与注药前比较,差异有统计学意义(P0.05)。注药后反流性食管炎内镜分级提高、临床症状明显改善。结论聚桂醇注射于LES区及膈裂孔区可改善食管内酸性环境,降低酸暴露时间,提高食管下段括约肌的压力,可在短时间内有效的改善临床症状,提高非手术治疗者的生存质量。     

A "room-temperature" injection molding/particulate leaching approach for fabrication of biodegradable three-dimensional porous scaffolds

A "room-temperature" injection molding approach combined with particulate leaching (RTIM/PL) has been, for the first time, developed in this work to fabricate three-dimensional porous scaffolds composed of biodegradable polyesters for tissue engineering. In this approach, a "wet" composite of particulate/polymer/solvent was used in processing, and thus the injection was not performed at melting state. Appropriate viscosity and flowability were facilely obtained at a certain solvent content so that the composite was able to be injected into a mould under low pressure at room temperature, which was very beneficial for avoiding thermal degradation of polyesters. As a demonstration, tubular and ear-shaped porous scaffolds were fabricated from biodegradable poly(D,L-lactide-co-glycolide) (PLGA) by this technology. Porosities of the resulting scaffolds were as high as 94%. The pores were well interconnected. Besides the well-known characteristics of injection molding to be suitable for automatization of a fabrication process with high repeatability and precision, this RTIM/PL approach is much suitable for tailoring highly porous foams with its advantages flexible for shaping complicated scaffolds, free of thermal degradation and high-pressure machine, etc.     

登革2型病毒ZS01/01株病毒样颗粒免疫原性研究

目的 研究登革2型病毒（Dengue virus type 2,DENV-2）病毒样颗粒（virus-Like particles,VLPs）的免疫原性.方法 利用已构建的DENV-2 ZS01/01株病毒样颗粒的表达质粒转染293T细胞,对分泌型VLPs进行大量培养并通过蔗糖密度梯度离心法对其进行纯化.纯化的VLPs经Western Blot及透射电镜观察等方法鉴定后免疫BALB/c小鼠.利用ELISA及中和试验等方法对体液免疫反应进行检测,ELISPOT法测定细胞免疫水平.结果 登革2型病毒样颗粒表达质粒转染哺乳动物细胞所得上清经蔗糖密度梯度离心后,电镜下可观察到类似于天然登革病毒的大小在45～55nm之间的病毒样颗粒.体液及细胞免疫检测结果显示登革2型VLPs可以刺激小鼠产生较高水平的登革E蛋白特异性抗体及一定水平的中和抗体,免疫小鼠脾淋巴细胞经体外刺激后IFN-γ水平显著升高.结论 登革2型病毒病毒样颗粒免疫BALB/c小鼠后可引起一定水平的细胞免疫及体液免疫反应,该研究结果为四价登革病毒样颗粒疫苗的研制奠定了基础.     

数据挖掘与医学数据资源开发利用

通过对目前医学数据资料状况的分析,提出了数据挖掘与医学数据资源开发利用的方法。文章首先介绍了聚类分析、关联分析、决策树三个数据挖掘方法及实例。实例资料结果说明通过分析疾病的分类、疾病与药物之间的关联,可以为疾病的早期预测,为相关疾病的诊疗路径提供帮助。因此聚类分析、关联分析以及决策树是医学数据挖掘中的主要方法,三者的结合可以对某一类疾病进行系统的分析。     

Effects of additives on the rheological properties and injectability of a calcium phosphate bone substitute material

An injectable calcium phosphate bone substitute material has been prepared by mixing amorphous calcium phosphate (ACP) and dicalcium phosphate dihydrate (DCPD) for use in noninvasive surgery, and the influence of additives, such as disodium hydrogen phosphate, polyethylene glycol (PEG), glycerin, and citric acid, on the rheological properties and injectability of the ACP + DCPD cement system have been studied in this work. Novel approach of thixotropy measurement has been used to characterize the stability of the pastes. The results show that the injectability and the setting time can be augmented by the addition of disodium phosphate solution to the paste but reduced by the addition of PEG 200, glycerin, or citric acid to the paste. This study suggests that the injectability and the setting time of the ACP + DCPD bone substitute material can be balanced, and the injectable calcium phosphate bone substitute material with satisfied fluidity and injectability for clinical operation can be prepared by optimizing the additives and their concentrations, according to different clinical requirements.