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991.
Nguyen K Bassez G Bernard R Krahn M Labelle V Figarella-Branger D Pouget J Hammouda el H Béroud C Urtizberea A Eymard B Leturcq F Lévy N 《Human mutation》2005,26(2):165
DYSF encoding dysferlin is mutated in Miyoshi myopathy and Limb-Girdle Muscular Dystrophy type 2B, the two main phenotypes recognized in dysferlinopathies. Dysferlin deficiency in muscle is the most relevant feature for the diagnosis of dysferlinopathy and prompts the search for mutations in DYSF. DYSF, located on chromosome 2p13, contains 55 coding exons and spans 150 kb of genomic DNA. We performed a genomic analysis of the DYSF coding sequence in 34 unrelated patients from various ethnic origins. All patients showed an absence or drastic decrease of dysferlin expression in muscle. A primary screening of DYSF using SSCP or dHPLC of PCR products of each of 55 exons of the gene was followed by sequencing whenever a sequence variation was detected. All together, 54 sequence variations were identified in DYSF, 50 of which predicting either a truncated protein or one amino-acid substitution and most of them (34 out of 54) being novel. In 23 patients, we identified two pathogenic mutations, while only one was identified in 11 patients. These mutations were widely spread in the coding sequence of the gene without any mutational "hotspot." 相似文献
992.
Identification of a novel coronavirus in patients with severe acute respiratory syndrome 总被引:1,自引:0,他引:1
Drosten C Günther S Preiser W van der Werf S Brodt HR Becker S Rabenau H Panning M Kolesnikova L Fouchier RA Berger A Burguière AM Cinatl J Eickmann M Escriou N Grywna K Kramme S Manuguerra JC Müller S Rickerts V Stürmer M Vieth S Klenk HD Osterhaus AD Schmitz H Doerr HW 《The New England journal of medicine》2003,348(20):1967-1976
993.
Adriana C. Gittenberger‐De Groot Edris A.F. Mahtab Nathan D. Hahurij Lambertus J. Wisse Marco C. Deruiter Maurits C.E.F. Wijffels Robert E. Poelmann 《Anatomical record (Hoboken, N.J. : 2007)》2007,290(1):115-122
Recent advances in the study of cardiac development have shown the relevance of addition of myocardium to the primary myocardial heart tube. In wild‐type mouse embryos (E9.5–15.5), we have studied the myocardium at the venous pole of the heart using immunohistochemistry and 3D reconstructions of expression patterns of MLC‐2a, Nkx2.5, and podoplanin, a novel coelomic and myocardial marker. Podoplanin‐positive coelomic epithelium was continuous with adjacent podoplanin‐ and MLC‐2a‐positive myocardium that formed a conspicuous band along the left cardinal vein extending through the base of the atrial septum to the posterior myocardium of the atrioventricular canal, the atrioventricular nodal region, and the His‐Purkinje system. Later on, podoplanin expression was also found in the myocardium surrounding the pulmonary vein. On the right side, podoplanin‐positive cells were seen along the right cardinal vein, which during development persisted in the sinoatrial node and part of the venous valves. In the MLC‐2a‐ and podoplanin‐positive myocardium, Nkx2.5 expression was absent in the sinoatrial node and the wall of the cardinal veins. There was a mosaic positivity in the wall of the common pulmonary vein and the atrioventricular conduction system as opposed to the overall Nkx2.5 expression seen in the chamber myocardium. We conclude that we have found podoplanin as a marker that links a novel Nkx2.5‐negative sinus venosus myocardial area, which we refer to as the posterior heart field, with the cardiac conduction system. Anat Rec, 290:115–122, 2007. © 2006 Wiley‐Liss, Inc. 相似文献
994.
995.
Ines Santisteban Francisco X. Arredondo-Vega Susan Kelly Marianne Debre Alain Fischer Jean Louis Prignon Bettina Hilman Jane Eldahr David H. Dreyfus Erwin W. Gelfand P. Lynne Howell Michael S. Hershfield 《Human mutation》1995,5(3):243-250
Three new missense mutations (H15D, A83D, and A179D) and a new splicing defect (573 + 1G→A) in the 5′ splice site of intron 5 were among six mutant adenosine deaminase (ADA) alleles found in three unrelated patients with severe combined immunodeficiency disease, the most common phenotype associated with ADA deficiency. When expressed in vitro, the H15D, A83D, and A179D proteins lacked detectable ADA activity. The splicing defect caused skipping of exon 5, resulting in premature termination of translation and a reduced level of mRNA. H15D is the first naturally occurring mutation of a residue that coordinates directly with the enzyme-associated zinc ion. Molecular modeling based on the atomic coordinates of murine ADA suggests that the D15 mutation would create a cavity or gap between the zinc ion and the side chain carboxylate of D15. This could alter the ability of zinc to activate a water molecule postulated to play a role in the catalytic mechanism. A83 and A179 are not directly involved in the active site, but are conserved residues located respectively in a helix 4 and β strand 4 of the α/β barrel. Replacement of these small hydrophobic Ala residues with the charged, more bulky Asp side chain may distort ADA structure and affect enzyme stability or folding.© 1995 wiley-Liss, Inc. 相似文献
996.
Clinical Features of Fatal Familial Insomnia: Phenotypic Variability in Relation to a Polymorphism at Codon 129 of the Prion Protein Gene 总被引:2,自引:0,他引:2
Pasquale Montagna Pietro Cortelli Patrizia Avoni Paolo Tinuper Giuseppe Plazzi Roberto Gallassi Francesco Portaluppi Jean Julien Claude Vital Marie Bernadette Delisle Pierluigi Gambetti Elio Lugaresi . 《Brain pathology (Zurich, Switzerland)》1998,8(3):515-520
Fatal Familial Insomnia is a hereditary prion disease characterized by a mutation at codon 178 of the prion protein gene cosegregating with the methionine polymorphism at codon 129 of the mutated allele. It is characterized by disturbances of the wake-sleep cycle, dysautonomia and somatomotor manifestations (myoclonus, ataxia, dysarthria, spasticity). PET studies disclose severe thalamic and additionally cortical hypometabolism. Neuropathology shows marked neuronal loss and gliosis in the thalamus, especially the medio-dorsal and anterior-ventral nuclei, olivary hypertrophy and some spongiosis of the cerebral cortex. Detailed analysis of 14 cases from 5 unrelated families showed that patients ran either a short (9.1+ 1.1 months) or a prolonged (30.8 + 21.3 months) clinical course according to whether they were homozygote met/met or heterozygote met/val at codon 129. Moreover, homozygotes had more prominent oneiric episodes, insomnia and dysautonomia at onset, whereas heterozygotes showed ataxia and dysarthria at onset, earlier sphincter loss and epileptic Grand Mai seizures; they also displayed more extensive cortical involvement on PET and at postmortem examination. Our data suggest that the phenotype expression of Fatal Familial Insomnia is related, at least partly, to the polymorphism at codon 129 of the prion protein-gene. 相似文献
997.
C. de Wit Christian Schäfer Philipp von Bismarck Steffen-Sebastian Bolz Ulrich Pohl 《Pflügers Archiv : European journal of physiology》1997,434(4):354-361
We studied whether a flow-independent increase of luminal wall shear stress (WSS) could dilate hamster arterioles in vivo
and which endothelial mediators are potentially involved. To this end the plasma viscosity was elevated by exchanging blood
for dextran-erythrocyte solution thereby augmenting WSS. Diameters of small and large arterioles as well as red blood cell
velocities were measured before and after exchange of blood for solutions of identical haematocrit containing either high-
(HMWD) or low-molecular weight dextran (LMWD). The potential role of endothelial autacoids was investigated by local application
of the NO-synthase inhibitor N
G-nitro-L-arginine (L-NNA), the inhibitor of cyclooxygenase, indomethacin (3 μM), or the K+-channel blocker, tetrabutylammonium (TBA, 0.1 mM) to assess the potential effects of EDHF. HMWD (n = 11 animals) increased plasma viscosity by 64 ± 3% and dilated arterioles of all branching orders (A1–A4) significantly
[by 24 ± 3% (A1–A2) and 32 ± 3% (A3–A4)]. This dilation compensated fully for the calculated initial increase of WSS. LMWD
(n = 6) did not affect plasma viscosity or arteriolar diameters. Tissue treatment with L-NNA (30–300 μM, n = 12) substantially diminished the HMWD-induced dilation in small arterioles (A3–A4; to 13 ± 3%; P<0.05) and virtually abolished it in large ones (A1–A2). Consequently, the calculated WSS increased significantly in these
arterioles (by 31 ± 5%). TBA combined with L-NNA (n = 4) did not reduce further the remaining dilation. Indomethacin (n = 6) had no effect on HMWD-induced dilation. We conclude that an increase of WSS induces a mainly NO-mediated arteriolar
dilation. This dilation occurs in all arteriolar branching orders and is of sufficient magnitude to compensate for the initial
WSS-increase. Thus, any elevations of WSS fulfil the requirement for a signal to change diameter along the arteriolar tree
in a coordinated manner. The fully compensating dilation which we observed indicates that WSS is a controlled variable. It
does, however, raise questions as to its role as a continuous endothelial stimulus.
Received: 2 August 1996 / Received after revision: 24 February 1997 / Accepted: 14 April 1997 相似文献
998.
Steffen Maiwald Helga Weißenborn Heike Windisch Christian Sommer Gert Müller Rudolf Taube 《Macromolecular chemistry and physics.》1997,198(10):3305-3315
The activation of the tris(allyl)neodymium complex Nd(η3-C3H5)3 · dioxane with alkylaluminoxanes (MAO or HIBAO) results in highly selective catalysts for the 1,4-cis-polymerization of butadiene (cis-selectivity up to 80%). Under standard conditions (50°C, toluene), the turnover frequency (TOF) of the catalyst/MAO system amounts to 10–15000 mol butadiene/(mol Nd · h). Molecular weight determinations indicate the formation of only one polymer chain per neodymium center as in a living polymerization reaction, and for the catalyst/HIBAO system the rate law rp = kp [Nd][C4H6] with kp = 8,7 · 10?2 mol/(L · s) (at 25°C) has been derived. As the catalytically active species, a cationic monobutenyl neodymium(III) complex is discussed, which is stabilized through coordinative interaction with the counter anion as well as the growing polybutadiene chain. This cationic complex reacts under insertion with butadiene in a bimolecular fashion. 相似文献
999.
Krister W. Fjermestad Rene Huster Christina Thunberg Simen Stokke Claus H. Gravholt Anne‐Kristin Solbakk 《American journal of medical genetics. Part C, Seminars in medical genetics》2020,184(2):482-492
A few studies have examined neuropsychological functions, sleep, and mental health combined in Klinefelter syndrome (KS; 47,XXY). We investigated neuropsychological functions with standard tests, sleep with actigraphy, and self‐reported mental health in 30 men with KS (Mean age = 36.7 years) compared to 21 controls (Mean age = 36.8 years). Men with KS scored significantly lower on mental speed, attention span, working memory, inhibition, and set‐shifting tests, as well as overall IQ (mean effect size difference Cohen's d = 0.79). Men with KS had significantly longer night wakes, with no differences in other sleep variables (mean d = 0.34). Men with KS reported poorer mental health than controls (mean d = 1.16). Regression analyses showed neuropsychological functions explained variance in some sleep domains for men with KS but not for controls. Neuropsychological functions explained variance in some mental health domains for controls. For men with KS, however, verbal IQ was the only significant predictor of mental health. Altogether, men with KS display problems in neuropsychological functions and mental health but do not appear different from controls on most sleep parameters. Our findings indicate that relations between neuropsychological functions, sleep, and mental health differ between men with KS and controls. 相似文献
1000.
J C Monboisse G Poulin P Braquet A Randoux C Ferradini J P Borel 《International journal of tissue reactions》1984,6(5):385-390
Fibrils of collagen reconstituted in vitro by dialysis against sodium formate are exposed to free oxy radicals generated by three different systems: (i) xanthine oxidase + hypoxanthine, (ii) gamma-rays originating from a cobalt bomb; (iii) pulse radiolysis in a particle accelerator. A degradation of the collagen fibres is demonstrated by determination of the amount of hydroxyproline-containing peptides in the supernatant after incubation. Types I and III collagen are sensitive to the effect, whereas type V collagen is not. The effect persists when collagen is specially delipidated. 相似文献