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31.
IntroductionRetroperitoneal fibrosis (RPF) is a rare disease associated with the formation of hard inflammatory and fibrous tissue in the retroperitoneum. Taking into consideration the fact that RPF is a rare disease with different subtypes, we compared the basal clinical and biochemical characteristics of the vascular and urorenal subtypes.Patients and methodsFrom January 2005 until December 2021, 27 patients were identified as vascular subtype (18 males) and 11 as urorenal subtype (9 males).ResultsPatients with a primary urorenal origin had significantly worse kidney function as reflected by serum creatinine and eGFR (both p < 0.001); they also had higher serum cholesterol (p < 0.01). Hypertension, diabetes, hyperlipidemia and nicotinism were significantly more prevalent in vascular subtype (all p < 0.001).ConclusionVascular subtype is more prevalent in our study with more cardiovascular risk factor present. Due to the diversity of symptoms, diagnosis of RPF becomes a challenge for specialists as well as therapy. 相似文献
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Tomasz Bartosik Joanna Drogosz-Stachowicz Anna Janecka Jacek Kdzia Barbara Pacholczyk-Sienicka Jacek Szymaski Katarzyna Gach-Janczak Tomasz Janecki 《Materials》2022,15(9)
In this report, we present efficient and stereoselective syntheses of 2,6-disubstituted trans-3-methylidenetetrahydropyran-4-ones and 2-(4-methoxyphenyl)-5-methylidenetetrahydropyran-4-one that significantly broaden the spectrum of the available methylidenetetrahydropyran-4-ones with various substitution patterns. Target compounds were obtained using Horner–Wadsworth–Emmons methodology for the introduction of methylidene group onto the pyranone ring. 3-Diethoxyphosphoryltetrahydropyran-4-ones, which were key intermediates in this synthesis, were prepared by fully or highly stereoselective addition of Gilman or Grignard reagents to 3-diethoxyphosphoryldihydropyran-4-ones. Addition occurred preferentially by axial attack of the Michael donors on the dihydropyranone ring. Relative configurations and conformations of the obtained adducts were assigned using a detailed analysis of the NMR spectra. The obtained methylidenepyran-4-ones were evaluated for cytotoxic activity against two cancer cell lines (HL-60 and MCF-7). 2,6-Disubstituted 3-methylidenetetrahydropyran-4-ones with isopropyl and phenyl substituents in position 2 were more cytotoxic than analogs with n-butyl substituent. Two of the most cytotoxic analogs were then selected for further investigation on the HL-60 cell line. Both analogs induced morphological changes characteristic of apoptosis in cancer cells, significantly inhibited proliferation and induced apoptotic cell death. Both compounds also generated DNA damage, and one of the analogs arrested the cell cycle of HL-60 cells in the G2/M phase. In addition, both analogs were able to inhibit the activity of topoisomerase IIα. Based on these findings, the investigated analogs may be further optimized for the development of new and effective topoisomerase II inhibitors. 相似文献
33.
Lake Szmaragdowe and Czarnog?owy Reservoir are both characterized by atypical origin as both were formed when quarries were flooded. The geological substrates of the two basins are unique, and they are both highly valued as recreational and tourist destinations. These two basins are among the most interesting in northwestern Poland. Studies of their thermal regimes confirmed that water mass mixing in Lake Szmaragdowe is bradymictic. The waters in the Czarnog?owy Reservoir mix entirely to a depth of 20 m, and this layer is also characterized by bradymixia. Deeper waters do not mix. This indicates that mixing in this basin is of the meromictic type. 相似文献
34.
Jan Marcin Węsławski Lucyna Kryla-Straszewska Jan Warzocha Jacek Urbański Maria Włodarska-Kowalczuk Lech Kotwicki 《Oceanological and hydrobiological studies.》2013,42(3):289-295
Extensive sampling (450 grabs) was performed all over the inner part of Puck Bay (105 km2 area) in summers of 2007–2009. The GIS-based analysis of samples was performed to assess in detail the distribution of 32 benthic species. The minimum area of occurrence was less than 1 km2 for Lekanosphaera rugicauda and the maximum was 83 km2 for Cerastoderma glaucum. The material reveals that species with the pelagic larval stage were most widespread, with the least distance between individuals and the highest average density (e.g. Cerastoderma glaucum, Hydrobia ventrosa). The most isolated and the least dense species within the studied area were discretely mobile, non-larval crustaceans (e.g. Gammarus oceanicus and Lekanosphaera rugicauda), present at single sites with the largest distance from each other. We conclude that analysis of species distribution helps in understanding the threats to populations of marine invertebrates and marine spatial planning, through locating the isolated species and populations. 相似文献
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Jagoda Abramek Jacek Bogucki Marta Ziaja-Sołtys Andrzej Stępniewski Anna Bogucka-Kocka 《Pharmacological reports : PR》2019,71(2):248-256
Background
Sodium dichloroacetate (DCA) is an agent with anticancer properties against solid tumors. DCA also seems to have antileukemic activity. In order to affirm it we investigate the effect of DCA on cell viability and apoptotic gene expression profiles in leukemia cell lines: CEM/C1, CCRF/CEM, HL-60, HL-60/MX2.Methods
Cell viability was assessed by trypan blue staining. The expression of 93 genes involved in the process of apoptosis was determined by real-time PCR method using Taqman Low Density Array (TLDA).Results
CEM/C1, CCRF/CEM, HL-60, HL-60/MX2 cells were exposed to DCA for 24?h. The sensitivity of each cell line to DCA is different and depends on the concentration. CEM/C1 was the most sensitive with an half-maximal inhibitory concentration (IC50) value of 30?mM, while HL-60/MX2 was the most resistant with an IC50 value of 75?mM. Exposure of leukemia cells to DCA causes differences in gene expression profiles which cannot indicate that any particular pathway of apoptosis is initiated. However, the presence of 388 statistically significant correlations between expression pattern of gens was determined.Conclusion
We showed that DCA causes a decrease in viability of leukemia cells. The decline depends on DCA concentration. The induction of any particular apoptosis pathway is not shown in cells after DCA treatment. For that reason, studies on the molecular mechanism of cell death after exposure to DCA should be continued. 相似文献39.
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