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131.
BACKGROUND: Immunofluorescence (IF) has been used in many laboratories for virus diagnosis but has begun to fall inappropriately out of favour as a diagnostic method as pressure on budgets and for objective quality control increases. OBJECTIVES: To review the status, value and benefits of IF. CONCLUSIONS: IF has, we believe, still a valuable role to play in routine virus diagnosis because it is rapid, accurate (with properly validated reagents), flexible and, by giving feedback on the quality of the specimens collected, promotes dialogue with the customer clinicians to their benefit and to that of the diagnostic laboratory. These benefits are not easily duplicated by other methods or techniques. While such rapid diagnosis primarily benefits the individual patient, providing results within a clinically relevant time has a secondary effect of increasing use of the service. It is our experience that the availability of rapid IF diagnosis (as opposed to culture or serology) for respiratory viral infections leads to a substantial increase in its use, thereby enhancing the amount and breadth of the resultant epidemiological data. 相似文献
132.
False-positive results in a recombinant severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by Western blotting with recombinant SARS-CoV spike polypeptide
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Woo PC Lau SK Wong BH Chan KH Hui WT Kwan GS Peiris JS Couch RB Yuen KY 《Journal of clinical microbiology》2004,42(12):5885-5888
Using paired serum samples obtained from patients with illness associated with increases in anti-human coronavirus OC43 (HCoV-OC43) or anti-HCoV-229E antibodies, we examined the possibility of false-positive results detected in a recombinant severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) nucleocapsid protein immunoglobulin G enzyme-linked immunosorbent assay (ELISA). Three of the 21 and 1 of the 7 convalescent-phase serum samples from persons with increases in antibodies against HCoV-OC43 and HCoV-229E, respectively, tested positive by the recombinant SARS-CoV nucleocapsid protein-based ELISA. None of these samples were found to contain a specific antibody in the recombinant SARS-CoV spike polypeptide-based Western blot assay. 相似文献
133.
A. L. McCarthy J. S. Malik Peiris C. E. Taylor M. A. Green L. Sviland A. D. Pearson A. J. Malcolm 《Journal of clinical pathology》1992,45(6):542-544
An allogeneic transplant recipient developed severe graft versus host disease (GvHD) 48 days after transplantation that was concomitant with a cytomegalovirus (CMV) viraemia, from which she subsequently died. CMV infection was detected in blood by the polymerase chain reaction and later in tissue by immunohistochemical techniques. CMV should be considered in patients in whom GvHD does not respond to appropriate treatment, and this case suggests that herpes viruses may increase the severity of GvHD by synergistically enhancing the graft versus host reaction. 相似文献
134.
Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnosis of SARS coronavirus pneumonia 总被引:11,自引:0,他引:11
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Woo PC Lau SK Wong BH Tsoi HW Fung AM Chan KH Tam VK Peiris JS Yuen KY 《Journal of clinical microbiology》2004,42(5):2306-2309
We report the evaluation of recombinant severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) nucleocapsid protein enzyme-linked immunosorbent assay (ELISA)-based antibody tests for serodiagnosis of SARS-CoV pneumonia and compare the sensitivities and specificities of this ELISA for detection of immunoglobulin G (IgG), IgM, IgA, and their combinations with serum samples from 149 healthy blood donors who donated blood 3 years ago as controls and 106 SARS-CoV pneumonia patients in Hong Kong. The specificities of the ELISA for IgG, IgM, and IgA detection were 95.3, 96.6, and 96.6%, respectively, with corresponding sensitivities of 94.3, 59.4, and 60.4%, respectively. The present ELISA appears to be a sensitive test for serodiagnosis of SARS-CoV pneumonia, is much more economical and less labor-intensive than the indirect immunofluorescence assay, and does not require cultivation of SARS-CoV. 相似文献
135.
Development and Evaluation of an Epstein-Barr Virus (EBV) Immunoglobulin M Enzyme-Linked Immunosorbent Assay Based on the 18-Kilodalton Matrix Protein for Diagnosis of Primary EBV Infection 总被引:1,自引:3,他引:1
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K. H. Chan R. X. Luo H. L. Chen M. H. Ng W. H. Seto J. S. M. Peiris 《Journal of clinical microbiology》1998,36(11):3359-3361
A new immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) based on the recombinant Epstein-Barr virus (EBV) matrix protein was developed. Compared to indirect immunofluorescence for the detection of IgM antibody to the EBV capsid antigen on clinical specimens, the sensitivity and specificity of the new IgM ELISA were 96 and 96%, respectively. 相似文献
136.
137.
Vaccination of chickens against H5N1 avian influenza in the face of an outbreak interrupts virus transmission. 总被引:15,自引:0,他引:15
Trevor M Ellis Connie Y H C Leung Mary K W Chow Lucy A Bissett William Wong Yi Guan J S Malik Peiris 《Avian pathology》2004,33(4):405-412
Vaccination of chickens with a commercially available killed H5N2 vaccine was being evaluated as an additional tool to enhanced biosecurity measures and intensive surveillance for control of highly pathogenic avian influenza subtype H5N1 disease in Hong Kong in 2002. In December 2002 to January 2003, there were outbreaks of H5N1 disease in waterfowl in two recreational parks, wild water birds, several poultry markets and five chicken farms. In addition to quarantine, depopulation of the affected sheds and increased biosecurity, vaccination of the unaffected sheds and surrounding unvaccinated farms was undertaken on three farms. In at least two farms, infection spread to the recently vaccinated sheds with low rates of H5N1 mortality in sheds when the chickens were between 9 and 18 days post-vaccination. However, after 18 days post-vaccination no more deaths from H5N1 avian influenza occurred and intensive monitoring by virus culture on these farms showed no evidence of asymptomatic shedding of the virus. This provides evidence that H5 vaccine can interrupt virus transmission in a field setting. 相似文献
138.
Haogao Gu Samuel S.M. Cheng Pavithra Krishnan Daisy Y.M. Ng Lydia D.J Chang Gigi Y.Z. Liu Sammi S.Y. Cheuk Mani M.Y. Hui Mathew C.Y. Fan Jacob H.L. Wan Leo H.K. Lau Daniel K.W. Chu Vijaykrishna Dhanasekaran Malik Peiris Leo L.M. Poon 《Emerging infectious diseases》2022,28(1):247
We sequenced ≈50% of coronavirus disease cases imported to Hong Kong during March–July 2021 and identified 70 cases caused by Delta variants of severe acute respiratory syndrome coronavirus 2. The genomic diversity detected in Hong Kong was similar to global diversity, suggesting travel hubs can play a substantial role in surveillance. 相似文献
139.
H.K.E. Osman J.S.M. Peiris C.E. Taylor P. Warwicker R.F. Jarrett C.R. Madeley 《Journal of medical virology》1996,48(4):295-301
Fifty-six renal allograft recipients were studied prospectively for 3 months or longer after transplant. The polymerase chain reaction (PCR) was used to screen peripheral blood leucocyte (PBL) specimens for CMV, human herpesvirus 6 (HHV6) and human herpesvirus 7 (HHV7) DNA (DNAemia) in 67 healthy controls and in serial (fortnightly) PBL specimens from the 56 allograft recipients. None of the healthy controls had detectable CMV DNAemia, although HHV6 and HHV7 DNAemia was found in 7% and 9% of individuals respectively. In contrast, DNAemia due to CMV, HHV6 and HHV7 was found in 50%, 36% and 39% of patients respectively, at some time during the post-transplant period. Of the 28 patients who had CMV DNAemia, eight developed “CMV disease.” The risk of progression to “CMV disease” was increased in patients with concurrent DNAemia to all three viruses (relative risk 3.7; 95% CI 1.3–10.5). The relative risk of “CMV disease” for patients with concurrent CMV and HHV7 was also increased (RR = 3.5; 95% CI = 1.1–11.6), while the association between CMV and HHV6 was inconclusive (RR = 2.1; 95% CI = 0.7–6.6). The first 26 patients recruited to the study also had serial serum samples tested for antibody responses to the three viruses. “CMV disease” was associated with rising antibody titres to HHV7 (Fisher's exact test, P = 0.02), and weakly so with HHV6 (P = 0.07). It is concluded that in patients with CMV DNAemia, concurrent infection/reactivation of HHV7 (and possibly HHV6) is associated with an increased risk of progression to “CMV disease.” © 1996 Wiley-Liss, Inc. 相似文献
140.
Biology of regional body fat distribution: relationship to non-insulin-dependent diabetes mellitus 总被引:9,自引:0,他引:9
Our studies support the view that body fat distribution and the accompanying metabolic abnormalities could be exacerabated by variability in the androgenic/estrogenic balance. Sensitivity to the androgenic milieu might be initiated by an early developmental aberration in sexual dimorphism. The possible direct and indirect sites of interaction between androgenic activity and the abnormal metabolic pathways in upper body obesity are summarized in Figure 19. 相似文献