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排序方式: 共有6753条查询结果,搜索用时 15 毫秒
111.
Zhen Lu Angelica Ortiz Ioannis I. Verginadis Amy R. Peck Farima Zahedi Christina Cho Pengfei Yu Rachel M. DeRita Hongru Zhang Ryan Kubanoff Yunguang Sun Andrew T. Yaspan Elise Krespan Daniel P. Beiting Enrico Radaelli Sandra W. Ryeom J. Alan Diehl Hallgeir Rui Constantinos Koumenis Serge Y. Fuchs 《The Journal of clinical investigation》2021,131(10)
Intercellular biomolecule transfer (ICBT) between malignant and benign cells is a major driver of tumor growth, resistance to anticancer therapies, and therapy-triggered metastatic disease. Here we characterized cholesterol 25-hydroxylase (CH25H) as a key genetic suppressor of ICBT between malignant and endothelial cells (ECs) and of ICBT-driven angiopoietin-2–dependent activation of ECs, stimulation of intratumoral angiogenesis, and tumor growth. Human CH25H was downregulated in the ECs from patients with colorectal cancer and the low levels of stromal CH25H were associated with a poor disease outcome. Knockout of endothelial CH25H stimulated angiogenesis and tumor growth in mice. Pharmacologic inhibition of ICBT by reserpine compensated for CH25H loss, elicited angiostatic effects (alone or combined with sunitinib), augmented the therapeutic effect of radio-/chemotherapy, and prevented metastatic disease induced by these regimens. We propose inhibiting ICBT to improve the overall efficacy of anticancer therapies and limit their prometastatic side effects. 相似文献
112.
Theodoros Eleftheriadis Georgios Pissas Efi Yiannaki Dimitra Markala Spyridon Arampatzis Georgia Antoniadi Vassilios Liakopoulos Ioannis Stefanidis 《Human immunology》2013
Indoleamine 2,3-dioxygenase (IDO) suppresses adaptive immunity. T-cell proliferation and differentiation to effector cells require increased glucose consumption, aerobic glycolysis and glutaminolysis. The effect of IDO on the above metabolic pathways was evaluated in alloreactive T-cells. Mixed lymphocyte reaction (MLR) in the presence or not of the IDO inhibitor, 1-methyl-dl-tryptophan (1-MT), was used. In MLRs, 1-MT decreased tryptophan consumption, increased cell proliferation, glucose influx and lactate production, whereas it decreased tricarboxylic acid cycle activity. In T-cells, from the two pathways that could sense tryptophan depletion, i.e. general control nonrepressed 2 (GCN2) kinase and mammalian target of rapamycin complex 1, 1-MT reduced only the activity of the GCN2 kinase. Additionally 1-MT treatment of MLRs altered the expression and/or the phosphorylation state of glucose transporter-1 and of key enzymes involved in glucose metabolism and glutaminolysis in alloreactive T-cells in a way that favors glucose influx, aerobic glycolysis and glutaminolysis. Thus in alloreactive T-cells, IDO through activation of the GCN2 kinase, decreases glucose influx and alters key enzymes involved in metabolism, decreasing aerobic glycolysis and glutaminolysis. Acting in such a way, IDO could be considered as a constraining factor for alloreactive T-cell proliferation and differentiation to effector T-cell subtypes. 相似文献
113.
Vasileios Boulougouris Ioannis Malogiannis George Lockwood Iannis Zervas Giuseppe Di Giovanni 《Experimental brain research. Experimentelle Hirnforschung. Expérimentation cérébrale》2013,230(4):605-624
Many studies have provided important information regarding the anatomy, development and functional organization of the 5-HT system and the alterations in this system that are present within the brain of the suicidal patient. There is also a growing interest in genetic factors associated with suicide, since these may lead to the emergence of personality traits that prove to be long-term predictors of suicidal behaviour. This review will focus on presenting the scientific literature on the role of the serotonergic system in suicidal behaviour as well as dysfunctional attitudes and personality traits associated with the suicidal patient. The association of the serotonin transporter gene, the 5-HT2 receptors and its metabolite 5-hydroxyindoleacetic acid with suicidal behaviour and animal models that may capture the complexity of suicidal behaviour will be discussed. Finally, the relationship between neurobiological models and psychotherapeutic interventions for suicide prevention will be considered with a focus on Schema Therapy (an approach that has shown particular promise in the treatment of suicidal individuals with personality disorders), aiming to invite the reader to integrate some aspects of the neurobiology of human suicidal behaviour into a model of suicide that can be used in a clinical encounter. 相似文献
114.
Fotinie Ntziora Dimitrios Paraskevis Catherine Haida Emanuel Manesis George Papatheodoridis Spilios Manolakopoulos Ioannis Elefsiniotis Timokratis Karamitros Alexis Vassilakis Angelos Hatzakis 《Journal of clinical microbiology》2013,51(9):2893-2900
Resistance to antiviral treatment for chronic hepatitis B virus (HBV) has been associated with mutations in the HBV polymerase region. This study aimed at developing an ultrasensitive method for quantifying viral populations with all major HBV resistance-associated mutations, combining the amplification refractory mutation system real-time PCR (ARMS RT-PCR) with a molecular beacon using a LightCycler. The discriminatory ability of this method, the ARMS RT-PCR with molecular beacon assay, was 0.01 to 0.25% for the different HBV resistance-associated mutations, as determined by laboratory-synthesized wild-type (WT) and mutant (Mut) target sequences. The assay showed 100% sensitivity for the detection of mutant variants A181V, T184A, and N236T in samples from 41 chronically HBV-infected patients under antiviral therapy who had developed resistance-associated mutations detected by direct PCR Sanger sequencing. The ratio of mutant to wild-type viral populations (the Mut/WT ratio) was >1% in 38 (63.3%) of 60 samples from chronically HBV-infected nucleos(t)ide analogue-naive patients; combinations of mutations were also detected in half of these samples. The ARMS RT-PCR with molecular beacon assay achieved high sensitivity and discriminatory ability compared to the gold standard of direct PCR Sanger sequencing in identifying resistant viral populations in chronically HBV-infected patients receiving antiviral therapy. Apart from the dominant clones, other quasispecies were also quantified. In samples from chronically HBV-infected nucleos(t)ide analogue-naive patients, the assay proved to be a useful tool in detecting minor variant populations before the initiation of the treatment. These observations need further evaluation with prospective studies before they can be implemented in daily practice. 相似文献
115.
116.
Morales Mojica Cristina M. Velazco-Garcia Jose D. Pappas Eleftherios P. Birbilis Theodosios A. Becker Aaron Leiss Ernst L. Webb Andrew Seimenis Ioannis Tsekos Nikolaos V. 《Journal of digital imaging》2021,34(4):1014-1025
Journal of Digital Imaging - The recent introduction of wireless head-mounted displays (HMD) promises to enhance 3D image visualization by immersing the user into 3D morphology. This work... 相似文献
117.
Understanding the mode of action of pathogenic bacteria through in vitro studies can provide additional insight into their infection strategies. Here we have characterized the effect of Photorhabdus luminescens and Photorhabdus asymbiotica on two distinct insect cell lines. We report that insect cell survival and metabolism as well as bacterial proliferation differ between infection with two Photorhabdus species. These findings reinforce the notion that P. luminescens and P. asymbiotica deploy diverse tactics to infect insect cells. This knowledge might lead to better appreciation of the interaction between pathogenic bacteria and different types of insect cells. 相似文献
118.
Gavriatopoulou Maria Ntanasis-Stathopoulos Ioannis Korompoki Eleni Fotiou Despina Migkou Magdalini Tzanninis Ioannis-Georgios Psaltopoulou Theodora Kastritis Efstathios Terpos Evangelos Dimopoulos Meletios A. 《Clinical and experimental medicine》2021,21(2):167-179
Clinical and Experimental Medicine - The new type of coronavirus (COVID-19), SARS-CoV-2 originated from Wuhan, China and has led to a worldwide pandemic. COVID-19 is a novel emerging infectious... 相似文献
119.
Brokos Ioannis Stavridakis Minos Lagouvardos Panos Krejci Ivo 《Odontology / the Society of the Nippon Dental University》2021,109(3):615-624
Odontology - Recording fluorescence using flash photography, may help reduce time of capture and apply effectively in clinical practice. To test methods for visualizing composite resins... 相似文献
120.
Nonenhanced arterial spin labeled carotid MR angiography using three‐dimensional radial balanced steady‐state free precession imaging 下载免费PDF全文