紫草素抗炎及对白三烯B4生物合成的抑制作用

皮下注射紫草素(shikonin)10mg.kg^-1对小鼠巴豆油耳炎症和大鼠酵母性足肿有明显抑制作用;在白细胞体外温孵系统中,紫草素在10^-4～10^-7mol·L^-1浓度范围内可浓度依赖性地抑制LTB₄和5-HETE生物合成,其50%抑制浓度(IC₅₀)分别为6.2×10^-7mol.L^-1和2.6×1O^-7mol·L^-1。此外,还报道紫草素几个天然衍生物对LTB₄生物合成的影响。     

旋光活性内酯化合物的研究:(—)MALYNGOLIDE的全合成

以己二酸为起始原料,成功地合成了有手性季碳中心的内酯化合物(—)-Mialyngolide(1),其中关键的步骤采用手性相转移催化剂进行立体选择烷基化反应,此合成路线短,总收率为10.5%。     

兔体内静脉输注乙酰普鲁卡因胺的药动学—药效学结合模型分析

用药动学-约效学结合模型,对ⅳ乙酰普鲁卡因胺(NAPA)后,进行了药代动力学和药效动力学分析。兔体内NAPA ⅳ后与静脉推注后的药动学参数基本一致;但效应按QT_c延长作为指标,其药效动力学的个别参数有显著性差异。输注后,NAPA的E_max,K_eo,S和EC₅₀分别为120±13.2ms,0.0182±0.007min^-1,2.26±0.93和6.31±0.71μg/ml;推注后,分别为53.6±2.5ms,0.061±0.017min^-1,2.19±0.39和6.21±1.74μg/ml。     

旋光活性内酯化合物的研究：（—）MALYNGOLIDE的全合成

以己二酸为起始原料，成功地合成了有手性季碳中心的内酯化合物（—）－Ｍｉａｌｙｎｇｏｌｉｄｅ（１），其中关键的步骤采用手性相转移催化剂进行立体选择烷基化反应，此合成路线短，总收率为１０．５％。     

布地奈德都保短期加大剂量吸入治疗急性发作期儿童哮喘52例疗效观察

目的：观察哮喘急性发作期短期加大布地奈德都保用量与短期加用口服或静注糖皮质激素作疗效比较。方法：两组病例均应用常规剂量布地奈德都保,治疗组52例在急性发作期短期加用口服或静脉糖皮质激作比较。结果：两组病例的治疗总有效率均达100％,治疗半年后及治疗1年后的肺功能FEV1,FEF50％（呼气50％FVC时的流速）、PET经统计学处理无明显差异（P＞0．05）。结论：哮喘急性发作期短期加大布地奈德都保用量与短期加用口服或静注糖皮质激素可以取得相同效果。     

5-脂氧合酶促癌机制研究进展

5-脂氧合酶蛋白是花生四烯酸代谢途径中的一种关键酶,在恶性肿瘤的发生、发展过程中起了重要作用．抑制5-脂氧合酶及其产物的表达有可能预防和逆转恶性肿瘤的发生,本文结合国内外文献,就5-脂氧合酶分子生物学特征及促癌机制的研究进展作一综述．     

Structural and functional differentiation of sinusoidal endothelial cells during liver organogenesis in humans

During fetal life, human liver sinusoids, which differentiate between 4 and 12 weeks of gestation from capillaries of the septum transversum, must support an important hematopoietic function and acquire the structural and functional characteristics of adult sinusoids. To gain insight into their differentiation process, we studied the expression of (1) markers of continuous endothelia, absent from adult sinusoidal endothelial cells (PECAM-1, CD34, and 1F10); (2) functional markers of adult sinusoidal endothelial calls (CD4, 1CAM-1, CD32, and CD14); and (3) extracellular matrix components (laminin, tenascin, fibronectin, and thrombospondin) in 37 fetuses of different gestational ages. We identified two successive differentiation events. (1) An early structural differentiation, occurring from 5 to 12 weeks of gestation, was characterized by the loss of continuous endothelial cell markers and a reduction in the perisinusoidal amount of laminin and in the deposition of tenascin, fibronectin, and thrombospondin; at the end of this process, fetal liver sinusoids present structural characteristics comparable to those of the sinuses in adult hematopoietic bone marrow. (2) A later functional differentiation was characterized by the acquisition of the markers of adult sinusoidal endothelial cells, initiating at 10 weeks of gestation and completed by 20 weeks of gestation; this process likely contributes to adapt liver sinusoids to the specific functions of the adult hepatic tissue.