基于ZigBee的医用高压氧舱氧气浓度自动控制系统

为有效防止医用高压氧舱氧气浓度异常对人体造成的损害,设计基于ZigBee的医用高压氧舱氧气浓度自动控制系统.数据采集模块利用微处理器STM32F103C8T6接收各个采集点的氧浓度数据,经数据处理模块的ZigBee将处理结果传输给从机接收端的MSP430G2553单片机,MSP430G2553单片机采用自组织T-S模糊...     

职业足球运动员足踝关节无症状慢性损伤MR影像特点的初步研究

目的分析职业足球运动员足踝关节无症状慢性损伤的MR影像特点。方法募集2017年3月—5月间18名天津当地足球俱乐部的现役运动员作为研究对象,均可正常参加训练和比赛,且至本次检查6个月内均无足踝关节不适症状。采用3.0 T MRI进行踝关节扫描,观察每位运动员踝关节的骨质、韧带、肌腱情况。采用Fisher精确检验比较触球足和立足足踝关节不同结构损伤情况的发生率。结果骨质损伤主要表现为骨髓水肿、囊变、关节游离体形成及不规则骨突形成。其中骨髓水肿共12例17个踝关节(触球足10个,立足7个);3例3个踝关节(触球足1个,立足2个)可见关节游离体形成;7例7个踝关节(触球足5个,立足2个)出现距后三角骨。2例2个踝关节(触球足、立足各1个)可见距骨后突。触球足组和立足组间骨质损伤发生率差异均无统计学意义(均P0.05)。内侧三角韧带损伤5例共5个踝关节(触球足2个,立足3个);距腓前韧带损伤17例共25个踝关节(触球足8个,立足17个);跟腓韧带损伤9例12个踝关节(触球足5个,立足7个);下胫腓前韧带损伤1例1个踝关节(触球足);未见距腓后韧带、下胫腓后韧带损伤病例。立足组距腓前韧带损伤发生率高于触球足组(P=0.003)。其余韧带损伤发生率,2组间差异无统计学意义(均P0.05)。肌腱损伤主要表现为腱鞘积液,踇长屈肌腱腱鞘积液17例26个踝关节(触球足12个,立足14个);趾长屈肌腱腱鞘积液8例10个踝关节(触球足5个,立足5个);胫骨后肌腱腱鞘积液9例12个踝关节(触球足5个,立足7个);腓骨长短肌腱腱鞘积液4例4个踝关节(触球足2个,立足2个);趾长伸肌腱腱鞘积液2例2个踝关节(触球足1个,立足1个)。未发现胫骨前肌腱、踇长伸肌腱腱鞘积液及跟腱周围积液。触球足组和立足组间腱鞘积液发生率差异均无统计学意义(均P0.05)。结论职业足球运动员艰苦的训练和比赛容易造成踝关节反复创伤,主要特点为骨髓水肿、囊变,韧带损伤及腱鞘积液。由于双足在足球运动中功能不同,触球足和立足损伤特点亦不同。     

A comparison of arterial spin labeling perfusion MRI and DCE‐MRI in human prostate cancer

Perfusion MRI has the potential to provide pathophysiological biomarkers for the evaluating, staging and therapy monitoring of prostate cancer. The objective of this study was to explore the feasibility of noninvasive arterial spin labeling (ASL) to detect prostate cancer in the peripheral zone and to investigate the correlation between the blood flow (BF) measured by ASL and the pharmacokinetic parameters K^trans (forward volume transfer constant), k_ep (reverse reflux rate constant between extracellular space and plasma) and v_e (the fractional volume of extracellular space per unit volume of tissue) measured by dynamic contrast‐enhanced (DCE) MRI in patients with prostate cancer. Forty‐three consecutive patients (ages ranging from 49 to 86 years, with a median age of 74 years) with pathologically confirmed prostate cancer were recruited. An ASL scan with four different inversion times (TI = 1000, 1200, 1400 and 1600 ms) and a DCE‐MRI scan were performed on a clinical 3.0 T GE scanner. BF, K^trans, k_ep and v_e maps were calculated. In order to determine whether the BF values in the cancerous area were statistically different from those in the noncancerous area, an independent t‐test was performed. Spearman's bivariate correlation was used to assess the relationship between BF and the pharmacokinetic parameters K^trans, k_ep and v_e. The mean BF values in the cancerous areas (97.1 ± 30.7, 114.7 ± 28.7, 102.3 ± 22.5, 91.2 ± 24.2 ml/100 g/min, respectively, for TI = 1000, 1200, 1400, 1600 ms) were significantly higher (p < 0.01 for all cases) than those in the noncancerous regions (35.8 ± 12.5, 42.2 ± 13.7, 53.5 ± 19.1, 48.5 ± 13.5 ml/100 g/min, respectively). Significant positive correlations (p < 0.01 for all cases) between BF and the pharmacokinetic parameters K^trans, k_ep and v_e were also observed for all four TI values (r = 0.671, 0.407, 0.666 for TI = 1000 ms; 0.713, 0.424, 0.698 for TI = 1200 ms; 0.604, 0.402, 0.595 for TI = 1400 ms; 0.605, 0.422, 0.548 for TI = 1600 ms). It can be seen that the quantitative ASL measurements show significant differences between cancerous and benign tissues, and exhibit strong to moderate correlations with the parameters obtained using DCE‐MRI. These results show the promise of ASL as a noninvasive alternative to DCE‐MRI. Copyright © 2014 John Wiley & Sons, Ltd.     

组胺对脑细胞内游离钙浓度的影响及其机制

目的 观察组胺对脑细胞内游离钙浓度的影响，并初步探讨其作用机制。方法 利用荧光探针Ｆｕｒａ－２测定乳鼠脑细胞内游离钙的浓度。结果 组胺可明显升高脑细胞内游离钙浓度，而异丙嗪可以阻断组胺的这一效应。结论 组胺升高脑细胞内游离钙浓度是由Ｈ１受体介导的。     

Phytohemagglutinin-activated human T cells induce lethal graft-versus-host disease in cyclophosphamide and anti-CD122 conditioned NOD/SCID mice

Graft-versus-host disease (GVHD) is a common complication after allogeneic hematopoietic stem cell transplantation. Much of our knowledge regarding GVHD comes from experiments on the mouse hematopoietic system due to ethical and technical constraints. Thus, in vivo GVHD models of the human immune system are required. In this study, we report an effective and reliable protocol for xenogeneic GVHD (xeno-GVHD) model induction using NOD/SCID mice, in which mice underwent a conditioning regimen consisting of intraperitoneal injection of cyclophosphamide and anti-CD122, followed by transfusion of phytohemagglutinin-activated human peripheral blood mononuclear cells containing 1?×?10⁷?T cells, which has not been reported previously. The present model can be utilized to study human immune cell function in vivo and elucidate the mechanisms underlying the pathogenesis of human GVHD. In addition, this model system can help researchers to rapidly determine whether proposed therapeutic strategies for GVHD are efficient in vivo and will elucidate the underlying mechanisms of drugs and cells to be investigated. Furthermore, such a protocol will undoubtedly be very helpful to laboratories that have no available sources of irradiation.     

浅析蜜丸内包材之优势

通过对蜜丸不同内包装材料应用优缺点分析,为不同品种内包材的选择和贮存期的确定提供参考。     

azithromycin的临床评价

口服国产ａｚｉｔｈｒｏｍｙｃｉｎ治疗细菌性感染１１０例，评价其疗效和安全性。治疗结果有效率为９３．６％（１０３／１１０），细菌清除率为９１．４％（９６／１０５）。不良反应发生率为５．４％（６／１１０），症状轻微，以胃肠道反应为主，个别患者出现一过性实验室异常。同时对国产ａｚｉｔｈｒｏｍｙｃｉｎ与进口品治疗呼吸道感染的疗效和安全性进行了随机对照观察，分别治疗５２例和５０例，结果两组的有效率及不良反应发生率均相仿     

miR-122-5p对滑膜肉瘤细胞株SW982增殖及放疗敏感性的影响

目的:探讨miRNA-122-5p对人滑膜肉瘤细胞株SW982增殖及放疗敏感性的影响.方法:采用脂质体法分别转染miR-122-5p mimic和inhibitor至SW982细胞.流式细胞术检测细胞周期及凋亡率,MTT法检测细胞活力.通过γ-射线干预细胞,分析其对放疗的敏感性.结果:与癌旁组织相比,滑膜肉瘤m iR-122-5p明显低表达,而且在转移者及低分化型中水平最低,而甲基化CpG结合蛋白2(MeCP2)的表达与之呈显著负相关(均P<0.05).与对照组相比,miR-122-5p mimic可上调miR-122-5p水平,下调MeCP2蛋白表达,抑制SW982细胞增殖,并增加SW982细胞对射线的放疗敏感性(均P<0.05);而过表达MeCP2却能阻断miR-122-5p的作用,增加细胞对放疗的抵抗(均P<0.05).结论:miR-122-5p在滑膜肉瘤中低表达,miR-122-5p/MeCP2轴参与调控肿瘤细胞增殖以及肿瘤细胞对γ-射线的放疗敏感性.