全文获取类型
收费全文 | 174122篇 |
免费 | 17387篇 |
国内免费 | 13335篇 |
专业分类
耳鼻咽喉 | 1437篇 |
儿科学 | 1677篇 |
妇产科学 | 1703篇 |
基础医学 | 20528篇 |
口腔科学 | 3080篇 |
临床医学 | 23482篇 |
内科学 | 26334篇 |
皮肤病学 | 1766篇 |
神经病学 | 9455篇 |
特种医学 | 6353篇 |
外国民族医学 | 127篇 |
外科学 | 17746篇 |
综合类 | 30963篇 |
现状与发展 | 56篇 |
一般理论 | 11篇 |
预防医学 | 11970篇 |
眼科学 | 5019篇 |
药学 | 18496篇 |
129篇 | |
中国医学 | 10243篇 |
肿瘤学 | 14269篇 |
出版年
2024年 | 657篇 |
2023年 | 3000篇 |
2022年 | 7335篇 |
2021年 | 9281篇 |
2020年 | 7003篇 |
2019年 | 5958篇 |
2018年 | 6147篇 |
2017年 | 5585篇 |
2016年 | 5320篇 |
2015年 | 8060篇 |
2014年 | 9945篇 |
2013年 | 8679篇 |
2012年 | 12946篇 |
2011年 | 14230篇 |
2010年 | 8765篇 |
2009年 | 6880篇 |
2008年 | 9109篇 |
2007年 | 8919篇 |
2006年 | 8734篇 |
2005年 | 8740篇 |
2004年 | 5670篇 |
2003年 | 5331篇 |
2002年 | 4526篇 |
2001年 | 3978篇 |
2000年 | 4166篇 |
1999年 | 4535篇 |
1998年 | 2918篇 |
1997年 | 2907篇 |
1996年 | 2198篇 |
1995年 | 2068篇 |
1994年 | 1868篇 |
1993年 | 1162篇 |
1992年 | 1518篇 |
1991年 | 1204篇 |
1990年 | 1038篇 |
1989年 | 936篇 |
1988年 | 821篇 |
1987年 | 697篇 |
1986年 | 607篇 |
1985年 | 472篇 |
1984年 | 231篇 |
1983年 | 172篇 |
1982年 | 110篇 |
1981年 | 112篇 |
1980年 | 66篇 |
1979年 | 92篇 |
1978年 | 15篇 |
1977年 | 14篇 |
1964年 | 7篇 |
1936年 | 8篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
71.
72.
73.
Yaobin Ouyang Gongmeizi Liu Wenting Xu Zhen Yang Nianshuang Li Chuan Xie Chun Zhou Jiang Chen Yin Zhu Junbo Hong Nonghua Lu 《Oncology Letters》2021,21(2)
Helicobacter pylori (H. pylori) is a main risk factor for gastric cancer (GC). Epithelial-mesenchymal transition (EMT) is involved in the development and progression of H. pylori-associated GC. However, the exact molecular mechanism of this process remains unclear. The AKT/GSK3β signaling pathway has been demonstrated to promote EMT in several types of cancer. The present study investigated whether H. pylori infection induced EMT, and promoted the development and metastasis of cancer in the normal gastric mucosa, and whether this process was dependent on AKT activation. The expression levels of the EMT-associated proteins, including E-cadherin and N-cadherin, were determined in 165 gastric mucosal samples of different disease stages by immunohistochemical analysis. The expression levels of E-cadherin, N-cadherin, AKT, phosphorylated (p-)AKT (Ser473), GSK3β and p-GSK3β (Ser9) were further determined in H. pylori-infected Mongolian gerbil gastric tissues and cells co-cultured with H. pylori by immunohistochemical analysis and western blotting. The results indicated that the expression levels of the epithelial marker E-cadherin were decreased, whereas the expression levels of the mesenchymal marker N-cadherin were increased during gastric carcinogenesis. Their expression levels were associated with H. pylori infection. Furthermore, H. pylori infection resulted in downregulation of E-cadherin expression and upregulation of N-cadherin expression in Mongolian gerbils and GES-1 cells. In addition, an investigation of the associated mechanism of action revealed that p-AKT (Ser473) and p-GSK3β (Ser9) were activated in GES-1 cells following co-culture with H. pylori. Furthermore, following pretreatment of the cells with the AKT inhibitor VIII, the expression levels of E-cadherin, N-cadherin, p-AKT and p-GSK3β did not show significant differences between GES-1 cells that were co-cultured with or without H. pylori. The levels of p-AKT and p-GSK3β were increased in H. pylori-infected Mongolian gerbils. In conclusion, the present study demonstrated that H. pylori infection activated AKT and resulted in the phosphorylation and inactivation of GSK3β, which in turn promoted early stage EMT. These effects were AKT-dependent. This mechanism may serve as a prerequisite for GC development. 相似文献
74.
Sheng Zhang Yan Wang Jie Xu Bokyung Kim Wenbin Deng Fuzheng Guo 《The Journal of neuroscience》2021,41(2):251
The developing CNS is exposed to physiological hypoxia, under which hypoxia-inducible factor α (HIFα) is stabilized and plays a crucial role in regulating neural development. The cellular and molecular mechanisms of HIFα in developmental myelination remain incompletely understood. A previous concept proposes that HIFα regulates CNS developmental myelination by activating the autocrine Wnt/β-catenin signaling in oligodendrocyte progenitor cells (OPCs). Here, by analyzing a battery of genetic mice of both sexes, we presented in vivo evidence supporting an alternative understanding of oligodendroglial HIFα-regulated developmental myelination. At the cellular level, we found that HIFα was required for developmental myelination by transiently controlling upstream OPC differentiation but not downstream oligodendrocyte maturation and that HIFα dysregulation in OPCs but not oligodendrocytes disturbed normal developmental myelination. We demonstrated that HIFα played a minor, if any, role in regulating canonical Wnt signaling in the oligodendroglial lineage or in the CNS. At the molecular level, blocking autocrine Wnt signaling did not affect HIFα-regulated OPC differentiation and myelination. We further identified HIFα–Sox9 regulatory axis as an underlying molecular mechanism in HIFα-regulated OPC differentiation. Our findings support a concept shift in our mechanistic understanding of HIFα-regulated CNS myelination from the previous Wnt-dependent view to a Wnt-independent one and unveil a previously unappreciated HIFα–Sox9 pathway in regulating OPC differentiation.SIGNIFICANCE STATEMENT Promoting disturbed developmental myelination is a promising option in treating diffuse white matter injury, previously called periventricular leukomalacia, a major form of brain injury affecting premature infants. In the developing CNS, hypoxia-inducible factor α (HIFα) is a key regulator that adapts neural cells to physiological and pathologic hypoxic cues. The role and mechanism of HIFα in oligodendroglial myelination, which is severely disturbed in preterm infants affected with diffuse white matter injury, is incompletely understood. Our findings presented here represent a concept shift in our mechanistic understanding of HIFα-regulated developmental myelination and suggest the potential of intervening with an oligodendroglial HIFα-mediated signaling pathway to mitigate disturbed myelination in premature white matter injury. 相似文献
75.
随着对肿瘤热疗和肿瘤免疫微环境(TIME)的深入研究,近年来热疗对TIME的作用越来越受到学者们的重视。本文就目前国内外研究进展,对热疗与TIME中几类主要免疫细胞和免疫相关细胞因子的影响及作用机制作一综述。全面而透彻的了解热疗对TIME的调控作用,有助于为肿瘤治疗提供新的思路和方法。 相似文献
76.
77.
目的:统计分析中国县级及以上医疗机构青光眼亚专科建设、诊疗设备配备和服务提供情况,为进 一步推动我国青光眼防治工作提供理论依据。方法:调查研究。2015年对全国提供眼科服务的县级 及以上医疗机构通过网上填报的方式进行普查,采用描述性统计方法,对我国县级及以上医疗机构 青光眼亚专科建设、诊疗设备配备和服务提供情况进行系统整理和统计分析。结果:本次调查覆盖 全国6 341家县级及以上医疗机构,其中设立青光眼亚专科的医疗机构有672家(10.60%)。在青光眼 检查和治疗相关设备中,视力表配置率最高(99.30%),平均每家医疗机构配置3.28台;超声生物显 微镜配置率最低(11.50%),平均每家医疗机构配置0.13台。眼压测量、白内障手术和小梁切开术是 青光眼主要的诊疗服务类型。结论:我国县级及以上医疗机构青光眼亚专科建设亟待加强,诊疗设 备配备不全,诊疗服务能力需要全面提升。 相似文献
78.
79.
80.