大学生网络成瘾量表的初步编制

目的编制可供评估网络成瘾倾向的自评量表。方法采用自编《大学生网络成瘾量表》为研究工具对197名在校大学生为被试进行测试。结果①初步编制的《大学生网络成瘾量表》共46条目,包括2个分量表:网络成瘾倾向和网络成瘾类型分量表,其中网络成瘾倾向分量表分为成瘾症状成分(即成瘾症状、消极影响2个维度)和认知、情感体验成分(即内心体验、自我认知2个维度);网络成瘾类型分为网络关系成瘾、网络娱乐成瘾、信息搜集成瘾和网络性成瘾4个维度;②本量表具有较好的信度:成瘾倾向分量表及各维度的同质性信度为0·80~0·95,网络成瘾类型分量表为0·78,符合测量学标准;网络成瘾倾向分量表及各维度的重测信度除消极影响为0·67外,其他为0·75~0·88,网络成瘾类型分量表为0·74,也都基本达到心理测量学标准;③本量表成瘾倾向分量表的成瘾标志和消极影响维度与陈淑惠《中文网络成瘾量表》各个因子以及总分的相关(0·78~0·83)均显示有统计学意义,说明其具有良好的结构效度。结论该量表评价内容范围较宽、提供信息量较大,题目量适中,并且其信、效度较好,为后续研究打下了良好基础。     

胃癌组织p16基因蛋白表达的意义

目的　检测 p1 6基因蛋白在胃癌组织、癌旁组织中的表达及其分布特点 ,分析其与胃癌临床病理学特征及预后的关系 .方法　采用 S- P免疫组织化学法对 53例胃癌组织及 35例癌旁组织进行 p1 6蛋白的定位观察 .结果　各病理类型胃癌组织、癌旁组织均有 p1 6基因蛋白表达 .阳性率分别为 62 .3% (33/53)和 88.6% (31 /35) ,阳性细胞的棕黄色颗粒主要位于细胞核 .胃癌 p1 6基因蛋白表达与性别、年龄、肿瘤部位在统计学上无差异 (P>0 .0 5) ;而与组织学类型、病理分级、淋巴结转移、临床病理分期在统计学上有差异 (P<0 .0 5) .p1 6蛋白阳性者 5年生存率 51 .0 %高于 p1 6蛋白阴性者 2 0 .0 % (P<0 .0 5) .结论　 p1 6基因缺失和表达水平的改变与胃癌发生、发展密切相关 ,检测 p1 6基因蛋白表达可作为辅助临床判断胃癌的生物学行为及推测预后的指标     

B细胞淋巴瘤TIL-T的IL-2Rα基因突变及蛋白表达检测

目的 :检测B细胞淋巴瘤 (B NHL)中肿瘤浸润T细胞 (TIL T)的活化标记IL 2Rα(CD2 5 )的基因及蛋白表达 ,寻找TIL T在B NHL肿瘤微环境中存在意义的实验依据。方法 :将 19例B NHL提取RNA ,采用RT PCR和SSCP检测其IL 2Rα的Ⅳ Ⅷ外显子基因突变与否 ;19例B NHL采用细胞免疫化学染色进行CD3、CD4、CD8、CD2 0、CD2 5细胞免疫表型的分析 ;2 9例B NHL和 2 0例良性淋巴组织 ,采用冰冻切片免疫组化方法进行IL 2Rα(CD2 5 )蛋白表达的检测。结果 :SSCP显示 19例TIL -T的IL 2Rα未发现异常泳动条带。细胞免疫化学染色示B NHL中 5 2 .6 3% (10 /19例 )的CD2 5 +细胞少于 10 % ,TIL T(CD3+)与CD4相关 (r =0 .5 7,P <0 .0 1)并与CD2 5相关 (r =0 .5 0 ,P <0 .0 5 )。免疫组化示 86 .2 % (2 5 /2 9例 )的B NHL中CD2 5表达 (+/- )和 (+) ,且主要表达在TIL T细胞上 ,呈散在分布 ,阳性细胞少于T标记细胞。结论 :19例TIL T的IL 2Rα在RNA水平未发现基因异常 ;CD2 5蛋白在B NHL中呈弱表达趋势。提示部分TIL T不表达CD2 5 ,推测TIL T的IL 2Rα的表达在转录后机制中可能出现异常或TIL T存在活化障碍。     

Effects of Platelet Factor 4 on Expression of Bone Marrow Heparan Sulfate in Syngenic Bone Marrow Transplantation Mice

Platelet factor 4 ( PF4) is a negativehematopoietic factor.It can inhibit the prolifera-tion of endothelial cells and hematopoietic stem/progenitor cells,particularly megakaryoryocyticcells,reversibly[1] ,inhibit DNA synthesis,blockcell cycle progression during S phase and reducethe sensibility of normal hematopoietic stem/pro-genitor cells,but not some cancer or leukemia celllines,to cytotoxic drugs and ionizing radia-tion[2 - 3] ,and it also can cause a population in-crease of the stem cel…     

赤芍总苷对沙土鼠全脑缺血再灌注损伤的保护作用

OBJECTIVE: To study the protective effects of the total paeony glycoside (TPG) against global cerebral ischemia-reperfusion injury in gerbils. METHODS: Gerbils models of global cerebral ischemia-reperfusion injury were prepared by bilateral common carotid artery ligation for 12 min followed by 24-hour reperfusion. The effects of TGP on brain edema index, superoxide dismatase (SOD) activity and malonaldehyde (MDA) concentration of the cerebral tissue homogenate and pathology of the brain were examined 24 h after model establishment. RESULTS: Compared with the model group, TPG at the doses of 200 and 400 mg/kg could significantly relieve brain edema, enhance SOD activity and lower MDA concentration in the gerbils. Pathological examination showed that the gerbils with TPG treatment had milder injury of the cells in the hippocampal CA1 region. CONCLUSIONS: TPG has obvious protective effects against global cerebral ischemia-reperfusion injury.     

Onychomycosis caused by Fusarium solani in a woman with diabetes

A case of untreated fusarial onychomycosis leading to serious consequences is reported. Fusarium solani is a widespread fungus and an occasional human pathogen. It usually invades rapidly in immunocompromised hosts, and often results in a poor outcome despite treatment. We report a woman with diabetes mellitus who had untreated fusarial infection of the nails, which developed into subcutaneous fusariosis, superinfected by bacteria, and then evolved into osteomyelitis that subsequently resulted in septic shock. Early management of mycotic nails in immunocompromised hosts is crucial to prevent life‐threatening disease.     

Hernia through the foramen of Winslow: a complication of cholecystectomy. A case report

In 151 years since first described, there have been 112 reported cases of hernia through the foramen of Winslow (HFW). All thus far have described HFW as a primary entity. The case reported appears to be unique with HFW as a surgical complication. HFW is the least common of internal hernias. The primary symptom is pain referred from the herniated organ and the hepatoduodenal ligament. An interesting sign is that the patient is found curled up or stooped over for pain relief. Anatomic factors implicated in HFW are an enlarged epiploic foramen, a floppy cecum and ascending colon, or abnormal length of small bowel mesentery. Tension on these structures causes pain with the torso extended. Distention of bowel in the lesser sac mimics gastric obstruction. Organs herniated are: small bowel (63%); cecum, ascending colon, and terminal ileum (30%); and transverse colon (7%). Of 25 cases reported since 1966, cecal herniation comprised two-thirds. The diagnosis may be made radiologically and the treatment is surgical. The case and a review of the literature are presented with attention to the anatomy. Also provided are the signs and symptoms of this interesting and perplexing diagnosis.     

Direct observation in vitro of how neuroblasts migrate: medulla and cochleovestibular ganglion of the chick embryo

The hypothesis that neuroblasts migrate in the nervous system by a locomotory process was tested experimentally. An in vitro preparation permitted direct observation of postmitotic cells migrating from the rhombic lip of the medulla and the anlage of the cochleovestibular ganglion. Cell locomotion was not seen. Instead migration was produced by elongation of a leading process, followed by translocation of the nucleus (perikaryal translocation). On the basis of comparisons with previous observations in situ, we propose that this represents a common mode of migration in the developing nervous system. Cell clusters were explanted from the rhombic lip at the developmental stage when they migrate from the ventricular zone to the acoustico-vestibular anlage in the medulla. Cells from the cochleovestibular ganglion were explanted after migration from the otocyst, but before ganglionic differentiation. Each neuroblast's migration route was formed by an elongating leading process ending in a growth cone. The growth cone attached to other cells and processes or ended freely on an acellular substrate. Nonneuronal cells usually migrated as has been described for fibroblasts, yet with some of the features of perikaryal translocation, but some nonneuronal precursor cells may migrate the way neuroblasts do. Neuroblasts did not migrate preferentially on the processes of nonneuronal cells, although the reverse could be observed. In fact a variety of interactions between migratory cells, neuronal and nonneuronal, were observed. The advantage of the experimental system described here is that one can observe cells migrating spontaneously at the times in development when they normally do so, while preserving the cellular populations present in situ.