全文获取类型
收费全文 | 2421篇 |
免费 | 123篇 |
国内免费 | 2篇 |
专业分类
耳鼻咽喉 | 172篇 |
儿科学 | 60篇 |
妇产科学 | 240篇 |
基础医学 | 235篇 |
口腔科学 | 58篇 |
临床医学 | 155篇 |
内科学 | 274篇 |
皮肤病学 | 43篇 |
神经病学 | 103篇 |
特种医学 | 107篇 |
外科学 | 328篇 |
综合类 | 319篇 |
一般理论 | 1篇 |
预防医学 | 215篇 |
眼科学 | 49篇 |
药学 | 98篇 |
1篇 | |
中国医学 | 2篇 |
肿瘤学 | 86篇 |
出版年
2021年 | 26篇 |
2019年 | 28篇 |
2018年 | 29篇 |
2016年 | 21篇 |
2015年 | 43篇 |
2014年 | 56篇 |
2013年 | 60篇 |
2012年 | 76篇 |
2011年 | 86篇 |
2010年 | 99篇 |
2009年 | 85篇 |
2008年 | 101篇 |
2007年 | 97篇 |
2006年 | 99篇 |
2005年 | 86篇 |
2004年 | 104篇 |
2003年 | 74篇 |
2002年 | 73篇 |
2001年 | 72篇 |
2000年 | 63篇 |
1999年 | 74篇 |
1998年 | 53篇 |
1997年 | 53篇 |
1996年 | 53篇 |
1995年 | 32篇 |
1994年 | 40篇 |
1993年 | 30篇 |
1992年 | 49篇 |
1991年 | 42篇 |
1990年 | 43篇 |
1989年 | 45篇 |
1988年 | 49篇 |
1987年 | 53篇 |
1986年 | 35篇 |
1985年 | 43篇 |
1984年 | 29篇 |
1983年 | 23篇 |
1982年 | 17篇 |
1981年 | 27篇 |
1980年 | 18篇 |
1979年 | 29篇 |
1978年 | 25篇 |
1977年 | 19篇 |
1976年 | 22篇 |
1975年 | 19篇 |
1974年 | 26篇 |
1973年 | 29篇 |
1970年 | 24篇 |
1969年 | 22篇 |
1966年 | 17篇 |
排序方式: 共有2546条查询结果,搜索用时 15 毫秒
51.
52.
Bailey R. House Joan B. Silk Joseph Henrich H. Clark Barrett Brooke A. Scelza Adam H. Boyette Barry S. Hewlett Richard McElreath Stephen Laurence 《Proceedings of the National Academy of Sciences of the United States of America》2013,110(36):14586-14591
Humans are an exceptionally cooperative species, but there is substantial variation in the extent of cooperation across societies. Understanding the sources of this variability may provide insights about the forces that sustain cooperation. We examined the ontogeny of prosocial behavior by studying 326 children 3–14 y of age and 120 adults from six societies (age distributions varied across societies). These six societies span a wide range of extant human variation in culture, geography, and subsistence strategies, including foragers, herders, horticulturalists, and urban dwellers across the Americas, Oceania, and Africa. When delivering benefits to others was personally costly, rates of prosocial behavior dropped across all six societies as children approached middle childhood and then rates of prosociality diverged as children tracked toward the behavior of adults in their own societies. When prosocial acts did not require personal sacrifice, prosocial responses increased steadily as children matured with little variation in behavior across societies. Our results are consistent with theories emphasizing the importance of acquired cultural norms in shaping costly forms of cooperation and creating cross-cultural diversity. 相似文献
53.
54.
55.
56.
Decreased expression of phospholipase C-beta 2 isozyme in human platelets with impaired function 总被引:4,自引:4,他引:4
Platelets from a patient with a mild inherited bleeding disorder and abnormal platelet aggregation and secretion show reduced generation of inositol 1,4,5-trisphosphate, mobilization of intracellular Ca2+, and phosphorylation of pleckstrin in response to several G protein mediated agonists, suggesting a possible defect at the level of phospholipase C (PLC) activation (see accompanying report). A procedure was developed that allows quantitation of platelet PLC isozymes. After fractionation of platelet extracts by high-performance liquid chromatography, 7 out of 10 known PLC isoforms were detected by immunoblot analysis. The amount of these isoforms in normal platelets decreased in the order PLC- gamma 2 > PLC-beta 2 > PLC-beta 3 > PLC-beta 1 > PLC-gamma 1 > PLC- delta 1 > PLC-beta 4. Compared with normal platelets, platelets from the patient contained approximately one-third the amount of PLC-beta 2, whereas PLC-beta 4 was increased threefold. These results suggest that the impaired platelet function in the patient in response to multiple G protein mediated agonists is attributable to a deficiency of PLC-beta 2. They document for the first time a specific PLC isozyme deficiency in human platelets and provide an unique opportunity to understand the role of different PLC isozymes in normal platelet function. 相似文献
57.
Signal transduction on platelet activation involves phosphoinositide- specific phospholipase C (PLC)-mediated hydrolysis of phosphatidylinositides and formation of inositol-1,4,5-triphosphate [I(1,4,5)P3], which mediates Ca2+ mobilization, and diacylglycerol (DG), which activates protein kinase C (PKC) to phosphorylate a 47-kD protein (Pleckstrin). We studied these events in two related patients previously reported (Blood 74:664, 1989) to have abnormal aggregation and 14C-serotonin secretion, and impaired intracellular Ca2+ mobilization in response to several agonists. Thrombin-induced I(1,4,5)P3 and phosphatidic acid formation were diminished. Pleckstrin phosphorylation was impaired on activation with thrombin, platelet- activating factor, and ionophore A23187, but was normal with PKC activator 1,2-dioctonyl-sn-glycerol (DiC8). Ca2+ mobilization induced by guanosine triphosphate (GTP) analog guanosine 5'-0-(3 thiotriphosphate) (GTP gamma S) was diminished. Pretreatment with either A23187 or DiC8 did not correct the impaired adenine diphosphate- induced secretion; however, upon stimulation with A23187 plus DiC8, pleckstrin phosphorylation and secretion were normal, indicating that both PKC activation and Ca2+ mobilization are essential for normal secretion. We conclude that these patients have a unique inherited platelet defect in formation of two key intracellular mediators [I(1,4,5)P3 and DG] and in the responses mediated by them due to a defect in postreceptor mechanisms of PLC activation. 相似文献
58.
Immunoglobulin V regions and the B cell 总被引:7,自引:2,他引:7
59.
Acquired granular pool defect in stored platelets 总被引:1,自引:0,他引:1
Platelets stored as concentrates (PC) for 72 h at 22 degrees C develop a functional defect. Alterations in adenine nucleotides of platelets have been shown to affect platelet function. Adenine nucleotide content of platelets was measured before and after storage and a decrease of 27.1 /+- 1.7% (mean /+- SE) in ATP and 39.1 /+- 2.6% in ADP were found in 34 PC stored with final volume of 50 ml. In 11 PC with 30 ml volume. ATP and ADP decreased by 39.4 /+- 3.2% and 49.4 /+- 2.1%, respectively. The mean ATP to ADP ratio of stored platelets was significantly higher than of fresh platelets in both groups, suggesting a relatively greater decrease in granular than metabolic pool nucleotides. Levels of low affinity platelet factor 4 measured by radioimmunoassay in plasma from 0.86 /+- 0.08 microgram/ml in the fresh PC to 8.59 /+- 0.39 microgram/ml in stored PC, indicating a concomitant alpha-granular secretion. Labeling of metabolic pool with 14C-adenine revealed a mean decrease in the adenylate energy charge of 2.0 /+- 0.4% in 12 of 16 stored PC, with a lower ATP and higher hypoxanthine labeling in stored as compared to fresh platelets. These observations suggest that stored platelets develop an acquired defect in both dense and alpha granules and in their ability to maintain ATP homeostasis. 相似文献
60.
Carr D House JS Kessler RC Nesse RM Sonnega J Wortman C 《The journals of gerontology. Series B, Psychological sciences and social sciences》2000,55(4):S197-S207
OBJECTIVES: This study examined whether psychological adjustment to widowhood is affected by three aspects of marital quality--warmth, conflict, and instrumental dependence-assessed prior to the loss. METHODS: The Changing Lives of Older Couples (CLOC) is a prospective study of a two-stage area probability sample of 1,532 married individuals aged 65 and older. The CLOC includes baseline data on marital quality and mental health and data on grief, anxiety, and depression collected 6, 18, and 48 months after spousal loss. RESULTS: Widowhood was associated with elevated anxiety among those who were highly dependent on their spouses and lower levels of anxiety among those who were not dependent on their spouses. Levels of yearning were lower for widowed persons whose relationships were conflicted at baseline and higher for those reporting high levels of marital closeness and dependence on their spouses. Women who relied on their husbands for instrumental support had significantly higher levels of yearning than men who depended on their wives. DISCUSSION: The findings contradict the widespread belief that grief is more severe if the marriage was conflicted and suggest a more complex relationship between bereavement and characteristics of the marriage. 相似文献