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61.
Houria Bourdache-Siguerdidjane 《Optimal control applications & methods.》1991,12(4):273-278
A direct analytical solution of angular velocities is given for regulating the angular momentum of a spacecraft using reaction jets only. The solution, which appears to be new, may be written down in terms of characteristic values and characteristic vectors. These values and vectors satisfy an algebraic non-linear equation. 相似文献
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Carrillo E Prados J Melguizo C Marchal JA Vélez C Serrano S Boulaiz H Mérida JA Aránega A 《Pathology international》2002,52(4):294-299
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Antibodies to Tat and Vpr in the GRIV cohort: differential association with maintenance of long-term non-progression status in HIV-1 infection. 总被引:2,自引:0,他引:2
Max W Richardson Jyotika Mirchandani Joseph Duong Sammy Grimaldo Virginia Kocieda Houria Hendel Kamel Khalili Jean Fran?ois Zagury Jay Rappaport 《Biomedicine & Pharmacotherapy》2003,57(1):4-14
The HIV-1 regulatory protein Tat and the accessory protein Vpr are thought to stimulate viral replication and contribute to viral pathogenesis as extracellular proteins. Humoral immune responses to these early viral proteins may therefore be beneficial. We examined serum anti-Tat and anti-Vpr IgG by ELISA in the GRIV cohort of HIV-1 seropositive slow/non-progressors (NP) and fast-progressors (FP), and in seronegative controls. Based on information obtained during a brief follow-up period (median = 20 months), NPs were sub-grouped as those maintaining non-progression status and therefore stable (NP-S), and those showing signs of disease progression (NP-P). As the primary comparison, initial serum anti-Tat and anti-Vpr IgG (prior to follow-up) were analyzed in the NP sub-groups and in FPs. Anti-Tat IgG was significantly higher in stable NP-S compared to unstable NP-P (P = 0.047) and FPs (P < 0.0005); the predictive value of higher anti-Tat IgG for maintenance of non-progression status was 92% (P = 0.029). In contrast, no-difference was observed in anti-Vpr IgG between NP-S and NP-P, although both were significantly higher than FPs (P = 0.001). Serum anti-Tat IgG mapped to linear epitopes within the amino-terminus, the basic domain and the carboxy-terminal region of Tat in stable NP-S. Similar epitopes were identified in patients immunized with the Tat-toxoid in a Phase I study in Milan. High titer serum anti-Tat IgG from both GRIV and Milan cohorts cross-reacted in ELISA with Tat from diverse viral isolates, including HIV-1 subtype-E (CMU08) and SIVmac251 Tat; a correlation was observed between anti-Tat IgG titers and cross-reactivity. These results demonstrate that higher levels of serum anti-Tat IgG, but not anti-Vpr IgG, are associated with maintenance of non-progression status in HIV-1 infection. Evidence that vaccination with the Tat toxoid induces humoral immune responses to Tat similar to those observed in stable non-progressors is encouraging for vaccine strategies targeting Tat. 相似文献
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Jose Prados Consolación Melguizo Raúl Ortiz Houria Boulaiz Esmeralda Carrillo Ana Segura Jose Juan Rodríguez‐Herva Juan Luis Ramos Antonia Aránega 《Experimental dermatology》2010,19(4):363-371
Please cite this paper as: Regression of established subcutaneous B16‐F10 murine melanoma tumors after gef gene therapy associated with the mitochondrial apoptotic pathway. Experimental Dermatology 2009. Abstract: Novel treatment modalities, including gene therapy, are needed for patients with advanced melanoma. We evaluated whether the gef gene, a suicide gene from Escherichia coli, had a significant cytotoxic impact on melanoma in vivo. First, we used a non‐viral gene delivery approach (pcDNA3.1/gef) to study the inhibition of melanoma cells (B16‐F10) proliferation in vitro. Secondly, we used direct intra‐tumoral injection of pcDNA3.1/gef complexed with jetPEI to deliver gef cDNA to rapidly growing murine melanomas. We demonstrated that gef gene not only has an antiproliferative effect on B16‐F10 cells in vitro, but also induces an important decrease in melanoma tumor volume (77.7% in 8 days) in vivo. Interestingly, after gef gene treatment, melanoma showed apoptosis activation associated with the mitochondrial pathway, suggesting that the induction of this death mechanism may be an effective strategy for its treatment. Our in vivo results indicate that gef gene might become a suitable therapeutic strategy for patients with advanced melanoma. 相似文献
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Raúl Ortiz Jose Prados Consolacion Melguizo Ana R. Rama Ana Segura Fernando Rodríguez-Serrano Houria Boulaiz Fidel Hita Antonio Martinez-Amat Roberto Madeddu Juan L. Ramos Antonia Aranega 《Journal of molecular medicine (Berlin, Germany)》2009,87(9):899-911
Novel treatment modalities, including gene therapy, are needed for patients with advanced melanoma. The E gene from the phage ϕX174 encodes a 91-aa protein which lyses Escherichia coli by formation of a transmembrane tunnel structure. To evaluate whether this E gene has a cytotoxic impact on melanoma cells in vitro and in vivo, and could therefore be used as a new therapeutic strategy
for this tumor type, we selected the B16-F10 murine melanoma cell line as a model. We used a nonviral gene delivery approach
(pcDNA3.1/E plasmid) to study the inhibition of melanoma cells' proliferation in vitro and direct intratumoral injection of
pcDNA3.1/E complexed with jetPEI to deliver E cDNA to rapidly growing murine melanomas, and found that the E gene has both a strong antiproliferative effect in B16-F10 cells in vitro and induces an efficient decrease in melanoma tumor
volume in vivo (90% in 15 days). Interestingly, the GFP-E fusion protein expressed in melanoma cells was located in the mitochondria.
In vitro and in vivo analysis demonstrated significant functional and morphological mitochondrial alterations accompanied
by a significant increase of cytochrome c and active caspase-3 and -9 in transfected cells, which suggests that tumoral cell death is mediated by the mitochondrial
apoptotic pathway. These results show that E gene expression in melanoma cells has an extraordinary antitumor effect, which means it may be a new candidate for an effective
strategy for melanoma treatment.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献