Compatible leaching effect of Concha Ostreae and some commonly-used Chinese medicinal materials]

OBJECTIVE: Based on a study of Concha Ostreae, to provide some reference for the rational approach to leaching mineral drugs. METHOD: Taking the change rates of compatible leaching and value of A as targets, the compatible leaching effect of Concha Ostreae with Radix et Rhizoma Rhei Praeparata, Radix Scutellariae, Pollen Typhae and Fructus Aurantii lmmaturus was studied. RESULT: If Concha Ostreae and the above cited medicinal herbs are decocted together, the yield of extract may be reduced to a certain extent, but the target composition of Radix et Rhizoma Rhei Praeparata and Fructus Aurantii lmmaturus remains intact, only the content of Radix Scutellariae baicalin may be influenced. CONCLUSION: The relationship of technology with preparation must be taken into consideration when making choice of the proper way to leach Concha Ostreae.     

刍论天灸疗法

天灸，又称自灸、发泡灸，是将某些对皮肤有刺激性的药物敷在皮肤一定的穴位或部位上，引起局部皮肤发泡，从而达到治疗疾病目的的一种中医外治疗法。     

银杏叶提取物EGb761通过caspase-3抑制TNF-α诱导HeLa细胞凋亡

目的探讨caspase-3的活化在银杏叶提取物EGb761对肿瘤坏死因子-α(TNF-α)诱导HeLa细胞凋亡中的影响.方法采用流式细胞术检测细胞凋亡,Western blot检测caspase-3 p20活性片段,Caspase-3 Colorimetric Assay试剂盒测定caspase-3活性.结果流式细胞术分析结果显示,EGb761在10～40 mg/L终浓度范围内对重组人肿瘤坏死因子-α(rhTNF-α)诱导的HeLa细胞凋亡均有显著的抑制作用(P＜0.01),呈剂量依赖关系;Western blot检测显示,rhTNF-α诱导的HeLa细胞caspase-3 p20活性片段水平增高,能被不同剂量EGb761(10～40 mg/L)明显抑制,且随EGb761浓度增加抑制效果增强;caspase-3活性测定结果显示,EGb761对rhTNF-α诱导的caspase-3活化有显著的抑制作用,且随剂量增加抑制作用增强.结论结果提示,EGb761抑制TNF-α诱导的HeLa细胞凋亡可能与其抑制caspase-3活化有关.     

杜氏盐藻基因组文库的构建

目的　构建杜氏盐藻基因组文库,旨在进一步分离和研究杜氏盐藻基因的结构和功能。方法　采用改进的SDS裂解法提取杜氏盐藻基因组DNA ,通过限制性内切酶Sau3AI进行部分酶切,用T4DNA连接酶将目的片段与噬菌体EMBL3载体臂进行连接后转染大肠杆菌LE3 92。结果　体外包装构建了杜氏盐藻基因组文库,得到重组子数为2 .2×10 4,经测定文库滴度为2 .2×10 5pfu/mL。结论　构建了杜氏盐藻基因组文库,为进一步筛选盐藻重要基因及其序列分析奠定基础。     

抗百日咳毒素单克隆抗体的纯化及应用研究

目的纯化抗百日咳毒素(PT)的单克隆抗体(M cAb),并建立特异、准确的PT定量检测方法。方法采用辛酸-硫酸铵沉淀法和A蛋白亲和层析法纯化杂交瘤细胞腹水,并经阻断抑制试验筛选识别不同表位的M cAb,用于建立定量检测PT的ELISA方法。结果经SDS-PAGE分析,纯化M cAb的纯度均在90%以上,选择二株识别不同抗原位点M cAbs,应用于检测PT的双抗夹心ELISA方法,灵敏度为2.14μg/L,批内变异系数5.85%,批间变异系数9.27%,平均回收率为108.12%,应用该法测定了国内几大生产厂家送检的无细胞百日咳疫苗原液中PT含量。结论获得了纯度高的抗PT M cAb,建立了一种特异、准确的定量检测PT的ELISA方法,并用于无细胞百日咳疫苗原液中PT含量的检测,为无细胞百日咳疫苗的质量控制提供了有力的手段。     

水中嗜肺军团菌分布规律研究

目的　探讨水环境中嗜肺军团菌分布规律及分型特征。方法　采集自然环境及生活环境中各主要水体样品 ,浓缩后经常规细菌培养后的疑似菌株 ,用血清学鉴定与 1 6SrRNA半套式PCR技术双重鉴定进行军团菌和嗜肺军团菌的分型。结果　全部 1 2 6个监测点水样有 2 8个出现军团菌阳性 (其中嗜肺军团菌 2 1个 ) ;自然环境及生活环境监测点军团菌阳性率分别为 32 1 %、2 2 2 % (其中嗜肺军团菌阳性率分别为2 1 4 %、1 6 7% ) ;全年 4 30份水样有 36份军团菌阳性 (其中 2 3份为嗜肺军团菌 ) ,夏、秋季检出率较高 ,春、冬季也有检出 ;空调冷却塔水、淋浴喷头水、公园湖水检出率较高。结论　嗜肺军团菌在自然环境及生活环境各水体中分布广泛。环境温度是军团菌污染的重要因素 ,夏、秋季是军团菌污染和军团病传播的高发季节 ,春、冬季也可发生。公园娱乐水体、空调冷却水、淋浴喷头水等与人群密切接触水体是军团菌污染和军团病传播的高危水体。应尽快采取预防与控制措施以保护广大人群身体健康     

硒对碘过量小鼠肝脏I型脱碘酶活性的影响

目的　研究碘过量摄入对小鼠肝脏I型脱碘酶 (IDI)活性的影响及硒的干预作用。方法　将 4 0只Balb c小鼠按体重随机分为 5组 :正常对照组、碘过量I组 (3mg L)、碘过量I+Se(1mg L)组、碘过量II组 (5mg L)以及碘过量II+Se(1mg L)组 ,分别喂饲碘过量水或碘过量 +硒水以及正常鼠饲料。 3个月后 ,取血、尿及脏器测定相关指标。结果　碘过量组小鼠出现了弥漫胶质性甲状腺肿 ;与正常对照组相比 ,两个碘过量的血清TT4水平显著升高 ,而碘过量II组血清TT3水平显著降低 ;碘可显著降低肝硒水平及I型脱碘酶活性 ,补硒可明显增加碘过量小鼠的肝硒储备 ,抑制碘过量I组脱碘酶活性的降低。结论　碘过量摄入降低小鼠肝硒水平及肝脏I型脱碘酶活性 ,可能是引起血清TT4升高、出现甲状腺肿的原因 ,补硒具有一定的干预作用。     

改良皮下埋藏缝线法重睑形成术

目的:探讨重睑形成术的手术方法和效果。方法:对15例(29眼)单睑者在皮下埋藏缝线法重睑形成的基本原理的基础上,在具体操作技术上用我们改良后的手术方法施行手术。结果:经5～10d和6～12mo的随访观察,除其中1例无力型因年龄大适应症选择不当0.5a后效果不佳;其余全部受术者,恢复后形成重睑皱褶自然,长短基本一致,重睑对称。受术者、施术者、第三者均较满意。结论:改良后的重睑形成术,手术操作简便,效果可靠。是一种具有应用价值的手术方式,值得推广使用。     

Psychometric evaluation of the Taiwan version of the Disability of the Arm, Shoulder, and Hand (DASH) questionnaire.

BACKGROUND AND PURPOSE: To adapt the Taiwan version of the Disability of the Arm, Shoulder, and Hand (DASH) questionnaire and evaluate its psychometric properties. METHODS: The DASH questionnaire was adapted through the process of translation, back-translation, and expert review. Eighty two subjects with upper extremity disorders were recruited in a medical center and 46 of these patients could be followed up to assess retest reliability in less than 10 days. Cronbach alpha and intraclass correlation coefficient were used to evaluate the internal consistency and test-retest reliability. Principal axis factor analysis was performed to assess the factor-construct validity, while concurrent validity was tested with the Medical Outcomes Study Short Form-36 (SF-36) Taiwan version questionnaire. RESULTS: The internal consistency of the Taiwan version of the DASH questionnaire was high (Cronbach alpha = 0.96) and the test-retest reliability was satisfactory (intraclass correlation coefficient = 0.9). Principal axis factor analysis confirmed the 1-factor model. The Pearson correlation coefficients of the DASH questionnaire to the SF-36 showed a correlation with physical component summary scores rather than mental component summary scores. Bodily pain, physical function and role-physical scores among the SF-36 subscales were most significantly correlated with DASH disability/symptom scores. CONCLUSION: The Taiwan version of the DASH questionnaire is a valid and reliable measure of health status for patients with upper-extremity disorders.     

中期妊娠引产硬膜外自控镇痛

目的　研究硬膜外自控镇痛 (PCEA)在中期妊娠引产中的镇痛效果及对产程、孕妇的影响。　方法　将 10 2例孕妇随机分为观察组和对照组。对照组除不给 PCEA外 ,其它产科处理措施与观察组相同。观察比较 2组的镇痛效果、产程时间、产后出血量。　结果　观察组的 Mulleetr评分明显低于对照组 ,产程时间短于对照组 ,差异有显著性 (P<0 .0 5 )。　结论　 PCEA用于引产镇痛产程时间短 ,对孕妇无不良影响 ,是一种理想的引产镇痛方法 ,值得临床推广应用。