OKT单克隆抗体检测单纯疱疹性角膜炎中药治疗前后外周血T细胞亚群的研究

为探讨单纯疱疹性角膜炎中药治疗前后外周血Ｔ细胞亚群变化。用ＯＫＴ单克隆抗体检测了１１２例单纯疱疹性角膜炎，随机分为中药治疗组６０例，西药对照组５２例。结果表明：中药治疗组治愈率８６６％，总有效率９８３３％；西药对照组治愈率７１１５％，总有效率９０３８％；两组差异有显著性意义（Ｐ＜００５）。外周血Ｔ细胞亚群：治疗前两组患者辅助性Ｔ细胞的比例明显低于抑制性Ｔ细胞（Ｐ＜００１）；中药治疗组辅助性Ｔ细胞治疗后较治疗前明显提高（Ｐ＞００５），而西药对照组治疗后辅助性Ｔ细胞较治疗前虽有提高，但无显著性差异（Ｐ＞０１）。认为单纯疱疹性角膜炎患者服中药后，机体内辅助性Ｔ细胞比值增加，细胞免疫功能增强。     

Cooperation of the inducible nitric oxide synthase and cytochrome P450 1A1 in mediating lung inflammation and mutagenicity induced by diesel exhaust particles

Diesel exhaust particles (DEPs) have been shown to activate oxidant generation by alveolar macrophages (AMs), alter xenobiotic metabolic pathways, and modify the balance of pro-antiinflammatory cytokines. In this study we investigated the role of nitric oxide (NO) in DEP-mediated and DEP organic extract (DEPE) -mediated inflammatory responses and evaluated the interaction of inducible NO synthase (iNOS) and cytochrome P450 1A1 (CYP1A1). Male Sprague-Dawley rats were intratracheally (IT) instilled with saline, DEPs (35 mg/kg), or DEPEs (equivalent to 35 mg DEP/kg), with or without further treatment with an iNOS inhibitor, aminoguanidine (AG; 100 mg/kg), by intraperitoneal injection 30 min before and 3, 6, and 9 hr after IT exposure. At 1 day postexposure, both DEPs and DEPEs induced iNOS expression and NO production by AMs. AG significantly lowered DEP- and DEPE-induced iNOS activity but not the protein level while attenuating DEPE- but not DEP-mediated pulmonary inflammation, airway damage, and oxidant generation by AMs. DEP or DEPE exposure resulted in elevated secretion of both interleukin (IL) -12 and IL-10 by AMs. AG significantly reduced DEP- and DEPE-activated AMs in IL-12 production. In comparison, AG inhibited IL-10 production by DEPE-exposed AMs but markedly increased its production by DEP-exposed AMs, suggesting that NO differentially regulates the pro- and antiinflammatory cytokine balance in the lung. Both DEPs and DEPEs induced CYP1A1 expression. AG strongly inhibited CYP1A1 activity and lung S9 activity-dependent 2-aminoanthracene mutagenicity. These studies show that NO plays a major role in DEPE-induced lung inflammation and CYP-dependent mutagen activation but a lesser role in particulate-induced inflammatory damage.     

导弹发射时脉冲噪声对豚鼠听器损伤的初步观察

本文报告小型战术导弹发射时的脉冲噪声对豚鼠听力的影响.我们观察了压力波峰值为143.6～177.0dB SPL,持续时间800～1500ms,频率范围50～8000Hz的强脉冲噪声对豚鼠听器的损伤.结果表明,压力波峰值小于150dB SPL时,26只豚鼠的听觉生理和病理指标未见异常,压力波峰值大于160dB SPL的30只豚鼠听器有不同程度的损伤.     

间质性肺疾病血清前胶原Ⅲ的初步研究

本文检测了２６例间质性肺疾病病人和１１例正常对照组血清Ⅲ型前胶原含量。结果表明，ＩＬＤ病人ＰＣⅢ含量显著高于对照组；认为血清ＰＣⅢ水平的改变可能作为话估肺内胶原代谢的一个指标。     

HER-2是否可以作为前列腺癌分子治疗的靶点?

目的 探讨HER-2在前列腺癌中的表达情况,探索以HER-2为靶点对中晚期前列腺癌进行靶向治疗的可行性,为对中国前列腺癌患者开展分子靶向治疗提供理论依据.方法 利用长春市人群前列腺癌筛查资料,应用临床资料统计、形态学观察、免疫组化染色及血清学检测等方法对164例前列腺癌组织标本及54例血清标本进行HER-2基因表达及血清含量分析.结果 (1) 164例前列腺癌组织标本中HER-2免疫组化染色结果均阴性;(2)54例血清标本中有32例可以检测出HER-2含量,其中有3例＞10ng/ml(5.6％).结论 HER-2在长春地区人群中前列腺癌的表达水平非常低,以HER-2作为治疗前列腺癌的靶点是不可行的.     

中部地区陆军驻训官兵消化系统疾病谱调查及其危险因素分析

目的 调查中部地区陆军驻训官兵消化系疾病患病情况,分析相关危险因素,初探消化系统疾病防治策略.方法 设计调查问卷,并通过预调查评估问卷质量;采用分层整群随机抽样方法,在我国中部地区7省市各抽取一个陆军基层单位,对所属的驻训官兵进行电子或纸质问卷调查,总结分析官兵消化系统疾病谱,并探讨疾病发生的危险因素.结果 共收集有效...     

WT1 mutation and podocyte molecular expression in a Chinese Frasier syndrome patient

We report on a Chinese girl with Frasier syndrome (FS). She presented with steroid-resistant focal segmental glomerulosclerosis (FSGS) and male pseudohermaphroditism. The WT1 IVS 9  +  5 G>A mutation was detected in one allele in the proband. The ratio of +KTS/−KTS was 0.67 in the proband’s cDNA. The expression of podocyte molecules (WT1, nephrin, podocin, α-actinin 4 and CD2AP) were also investigated in a renal specimen of this FS patient. WT1 expression showed diffuse nuclear staining, with less obvious speckles in the patient’s glomeruli than in those of controls. The distribution and intensity of podocyte molecules were altered both in normal- and abnormal-appearing glomeruli. In conclusion, the study presented a case of FS by clinical manifestation, renal pathology, karyotype analysis and genetic testing. A lower ratio of +KTS/−KTS and an abnormal distribution of WT1, as well as abnormal expressions of other podocyte molecules, were also revealed. The mechanisms of WT1 mutation causing FS still need to be investigated. Jianguo Li and Dan Zhao contributed equally to this work.     

Tübingen hip flexion splint for the treatment of developmental dysplasia of the hip in children younger than six months age: a meta-analysis

PurposeTo investigate the success rate of the Tübingen hip flexion splint (THFS) for the treatment of developmental dysplasia of the hip (DDH), of different severity as per the Graf classification, among infants younger than six months of age. The type and incidence rate of complications associated with THFS treatment were also evaluated.MethodsThe following databases were searched using keywords and limited for age less than six months: PubMed, Embase, Web of Science, Cochrane Library, and SinoMed, between inception and July 2020. Articles were screened and extracted by two researchers, and the quality of the included literature was evaluated (methodological index for non-randomized studies criteria). R studio 1.3 was used for statistical analysis. The review process was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines.ResultsAfter screening, eight articles were included in the analysis, contributing 1211 hips (875 patients). The overall success rate of THFS treatment is 91% (95% confidence interval (CI) 0.82 to 0.95). The success rate by Graf type is as follows: type-II, 98% (95% CI 0.94 to 1.00); type-III, 96% (95% CI 0.88 to 1.00); and type-IV, 32% (95% CI 0.18 to 0.47). Complications (24/1211, 2%) included transient femoral nerve palsy (n = 1); avascular necrosis of the femoral head (n = 9) and residual acetabular dysplasia (n = 14).ConclusionTHFS treatment is successful for Graf type-II and –III, but low for type-IV, with a low rate of complication. THFS may be an effective treatment option for DDH among infants less than six months of age. However, those with Graf type-IV require close monitoring.Level of EvidenceIII     

尾悬吊模拟失重对大鼠血清GAS含量和胃黏膜Hsp70及其基因表达的影响

目的研究尾悬吊模拟失重对大鼠血清GAS含量和腺胃区黏膜组织中Hsp70及其基因表达的影响。方法健康雄性Wistar大鼠64只,随机分成8组(n=8),按模拟失重时相分别为6h、12h、1d、2d、3d、5d、7d和0h(地面对照组)。采用尾吊法建立模拟失重动物模型。应用放免法检测各组大鼠血清GAS含量,免疫组化法和RT-PCR技术分别检测大鼠胃黏膜组织中Hsp70蛋白及Hsp70mRNA表达。结果与对照组相比,实验组大鼠血清GAS含量在模拟失重早期(6～12h)明显升高(P<0.05),随着尾悬吊时相的延长,血清GAS含量呈逐渐下降趋势。免疫组化染色结果显示,实验各组大鼠胃黏膜组织中均有Hsp70阳性表达;悬吊6h～2d各组大鼠胃黏膜组织中着色明显变深,尤其胞核在模拟失重早期即深染,期间胞浆染色亦增强;悬吊3～7d组大鼠的胞核染色逐渐变浅,部分胞核中染色消失,但细胞浆内仍有比较明显的Hsp70染色。RT-PCR结果显示,模拟失重6h大鼠胃黏膜组织中Hsp70mRNA表达水平即明显上调(P<0.05)。12h、1d、2d各组的Hsp70mRNA表达逐渐回落,3d组降至接近对照组水平,其后又出现一短时相升高现象。结论尾悬吊模拟失重可导致大鼠血清GAS含量波动和腺胃区胃黏膜组织中Hsp70及其基因表达明显改变,提示GAS和Hsp70在失重条件下胃黏膜应激反应及适应耐受过程中可能发挥重要作用。     

简易眶下壁骨折复位固定器的研制与临床应用

目的探讨严重爆裂性眶下壁骨折治疗中移位组织的复位固定方法。方法自行研制了简易眶下壁骨折复位固定器。此固定器手术方法简单,采用上颌窦根治术入路。治疗眶下壁骨折9例。结果所有患者骨折均复位,组织嵌颊消除,复视消失或减轻,眼球内陷≤2mm。结论简易眶下壁骨折复位固定器对爆裂性眶下壁骨折的治疗效果良好。