高效液相色谱法测定血浆中泮托拉唑浓度

 目的:建立用高效液相色谱法测定血浆中泮托拉唑浓度的方法,为进行泮托拉唑的体内药物代谢、生物利用度研究提供基础?方法:血浆用0.01mol·L^-1磷酸氢二钠做pH调节剂,二氯甲烷做提取溶剂,硝西泮作内标?采用Platinum C₁₈(5u,250mm×4.6mm)柱,流动相:甲醇-水-醋酸盐缓冲液(40∶60∶5),流速1.0ml·min^-1,检测波长286nm?结果:该法回收率平均在98.36%～104.27%,重现性高,RSD在4.693%～9.12%范围内(n=5),线性范围:20～4000μg·L^-1,最低检测浓度15μg·L^-1?结论:HPLC测定血浆中泮托拉唑的方法简便、准确、灵敏、专一,能满足血药浓度测定及药代动力学研究的需要?     

Effects of d-Galactosamine Hydrochloride and Partial Hepatectomy on Spontaneous Hepatic Injury and Hepatocarcinogenesis in Long-Evans Cinnamon Rats

To examine the effect of nongenotoxic chemicals on hepatocarcinogenesis in Long-Evans Cinnamon (LEC) rats, we gave 6-week-old male and female LEC rats ( n =18) weekly subcutaneous injections of d -galactosamine hydrochloride (GalN, 300 mg/kg) in 0.9% NaCl or only 0.9% NaCl for 50 weeks, and killed them in week 62. GalN-treated male rats unexpectedly showed no lethal necrotizing hepatitis. GalN treatment increased the incidence of cholangiofibrosis in males and its severity in females, but did not cause significant increases of hepatocellular tumors in either sex. GalN treatment increased the 5-bromo-2'-deoxyuridine (BrdU)-labeling index of hepatocytes and plasma hepatocyte growth factor, and accelerated megalocytic alterations without reduction of the hepatic copper concentration. Next, male and female LEC rats were subjected to two-thirds partial hepatectomy (PH) or sham hepatectomy in week 8 ( n =12) or in week 14 ( n =9), and killed in week 62. PH in week 14 inhibited lethal hepatitis, but PH in week 8 was less effective. PH reduced the hepatic copper concentration to half that of controls. The present data suggest that induction of hepatocyte regeneration by repeated injections of GalN, or by PH just before the onset of jaundice has a significant effect in prevention of hepatic injury of LEC rats, but not enhancement of spontaneous hepatocarcinogenesis.     

病毒性肝炎慢性活动性病理诊断的量化指标

目的：通过对肝组织切片中各种基本病理化分析,采用量化手段确定其在肝病诊断中的价值,建立肝脏病理量化诊断的标准。方法;选取基本病理变化１１项作为诊断的慢性指标,各赋于一定的分值,分值相加为一活动性指数,观察慢性肝炎肝组织切片７２２份,并与常规片及ＫｎｏｄｅｌｌＨＡＩ评分系统对比,采用方差分析和ｑ检验,结果;轻度慢性肝炎４０２例,中度慢性肝炎２１１例,重度慢性肝炎１０９例,三种疾病肝组织病理慢性活动性     

Two new cytotoxic cardenolides from the latex of Antiaris toxicaria

Two new cardenolides, toxicarioside F (1) and toxicarioside G (2), were isolated from the latex of Antiaris toxicaria (Pers.) Lesch (Moraceae). Their structures were elucidated on the basis of spectral data and chemical evidence. Compounds 1 and 2 showed significant cytotoxicity against K562, SGC-7901, SMMC-7721, and HeLa cell lines in vitro by the MTT method.     

温固化水凝胶负载骨髓基质干细胞修复兔关节骨软骨缺损的实验研究

目的：探讨温固化水凝胶作为骨髓基质干细胞的载体修复软骨缺损的可行性。方法：将培养的骨髓基质干细胞与400g／L浓度的温化水凝胶混合后移植到兔膝关节软骨缺损中，分别于术后4、8、12周观察大体标本及组织学修复结果。结果：实验组的缺损为透明软骨修复。而单纯材料组和空白对照组则以纤维组织修复。参考Wakitani制定的组织学评分标准，Pluronic F-127组和空白对照组的组织学评分在各个时期无显著的差异（P〉0．05），而实验组和空白组在各个时期均存在显著的差异（P〈0．05）。结论：Pluronic F-127可作为软骨组织工程良好的支架材料。     

5-BrdU标记的骨髓间充质干细胞对大鼠创面愈合的作用研究

目的探讨经5-溴脱氧尿嘧啶(5-BrdU)标记的骨髓间充质干细胞(BMSCs)在大鼠创面愈合中的作用.方法分离培养大鼠BMSCs并用流式细胞仪技术鉴定.将大鼠背部脊柱一侧作为实验组并制作一全层皮肤缺损模型,其对侧为对照组,经尾静脉注入经5-BrdU标记的BMSCs,移植细胞数为2×107个.术后3天、7天、14天、21天、28天分批处死动物,观察移植细胞参与创面愈合的情况.结果流式细胞仪鉴定细胞表达CD29、CD90表达阳性;CD45表达呈阴性.实验组经5-BrdU体外标记的BMSCs进行细胞移植14天后大鼠创面局部及边缘可见5-BrdU标记的阳性细胞聚集,免疫组化染色可见基底层、真皮层、新生血管周围有散在红棕色标记细胞;对照组中未发现明显的聚集现象;抗5-BrdU/FⅧ、5-BrdU/波形蛋白免疫双标技术检测结果显示在创伤部位有部分BMSCs分化为血管内皮细胞和成纤维细胞参与创面愈合.大鼠对侧正常皮肤中未见明显的BMSCs聚集和增殖分化情况.结论5-BrdU标记的BMSCs参与皮肤创面愈合.