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Neisser Deusch Weigert Kobrak Eckstein Rosenow Tobler Oppenheimer Holzer Finkenrath Dessecker A. W. Fischer Zuntz Peiper Simon Hoffa Eisner-Behrend Seligmann Braun Zinn Dietlen Levinger Kaufmann Reiss Schlesinger Hayward Desseker Eisner H. Hirschfeld Griesbach Dreyfuss Heinrich Davidsohn Freudenberg Jonas V. Jaschke Dietrich Teleky Schönberg 《Journal of molecular medicine (Berlin, Germany)》1922,1(18):910-917
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BACKGROUND: Cellular blood components are irradiated to prevent graft- versus-host disease in transfusion recipients at risk for this syndrome. Because gamma radiation can result in the production of reactive oxygen species, the role of reactive oxygen species was investigated in radiation-induced red cell damage. STUDY DESIGN AND METHODS: Whole blood from normal donors was exposed to various doses of t-butyl hydroperoxide (0-1 mM) and/or to gamma-radiation (0-50 Gy). Oxidative damage was assessed by the extent of lipid peroxidation (measured by thiobarbituric acid-reactive substances [TBARS]) and hemoglobin oxidation. Fresh blood was divided into three parts-one initially irradiated and stored, another stored with portions irradiated weekly, and a third stored without irradiation. TBARS and hemoglobin oxidation were measured weekly. RESULTS: As expected, t- butyl hydroperoxide induced TBARS formation and hemoglobin oxidation in a dose-dependent fashion. The gamma-radiation not only increased hemoglobin oxidation and TBARS formation, but also enhanced the t-butyl hydroperoxide effect on red cells. Red cell storage increased TBARS generation and hemoglobin oxidation in a time-dependent fashion. When radiation was administered either initially or after weekly storage, TBARS production and hemoglobin oxidation were increased over that measured in unirradiated paired controls. CONCLUSION: Gamma radiation at clinically used doses increases lipid peroxidation and hemoglobin oxidation in human red cells. The effect of gamma-radiation is accentuated by blood storage and induces damage independent of time of storage. 相似文献
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Routine invasive versus selective invasive strategies for Non‐ST‐elevation acute coronary syndromes: An Updated meta‐analysis of randomized trials 下载免费PDF全文
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Pre-B cells and other possible precursor lymphoid cell lines derived from patients with X-linked agammaglobulinemia 总被引:14,自引:6,他引:14 下载免费PDF全文
SM Fu JN Hurley JM McCune HG Kunkel RA Good 《The Journal of experimental medicine》1980,152(6):1519-1526
A group of unique Epstein-Barr virus-containing cell lines was derived from the bone marrow of three patients with X-linked agammaglobulinemia. Efforts to obtain cell lines from the peripheral blood of these patients were uniformly unsuccessful. Immunofluorescence analyses as well as biosynthetic studies with [(35)S]methionine indicated unusual patterns of Ig synthesis in many of these bone marrow derived lines. Seven of the lines were of particular interest in that two produced no Ig of any type; two others showed no Ig by fluorescence but small amounts by [(35)S]methionine labeling; one expressed only cytoplasmic μ chains without any evidence of light chain synthesis, and two produced primarily μ chains with only slight amounts of light chains. One of the lines without membrane or cytoplasmic Ig studied in detail grew like a typical lymphoid line and was carried in intermittent culture over a period of 2 yr without Ig expression. One line grew quite differently and resembled the round cell type described previously, which has been obtained from a variety of sources. The cell line with cytoplasmic μ chains and no light-chain expression had the characteristic properties of pre-B cells. Three normal type Ig-producing cell lines also were obtained from the patients. The accumulated evidence obtained in the present study indicates that these unusual cell lines represent normal precursor cells of the B-cell lineage; these grew out in these cases because of the virtual absence of mature B cells that ordinarily overgrow the culture system. However, the possibility that in certain instances they reflect abnormal Ig synthesis characteristic of the disease has not been ruled out. 相似文献
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Transcatheter closure of patent ductus arteriosus using the AMPLATZER™ duct occluder II (ADO II) 下载免费PDF全文
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Philipp C. Seppelt MD Silvia Mas-Peiro MD Roberta De Rosa MD PhD Isabell M. Murray MD Mani Arsalan MD Lars Holzer MD Gösta Lotz MD Patrick Meybohm MD Kai Zacharowski MD PhD Thomas Walther MD Andreas M. Zeiher MD Stephan Fichtlscherer MD Mariuca Vasa-Nicotera MD 《Catheterization and cardiovascular interventions》2021,97(1):E146-E153