全文获取类型
收费全文 | 3826篇 |
免费 | 194篇 |
国内免费 | 32篇 |
专业分类
耳鼻咽喉 | 15篇 |
儿科学 | 105篇 |
妇产科学 | 30篇 |
基础医学 | 443篇 |
口腔科学 | 77篇 |
临床医学 | 313篇 |
内科学 | 1052篇 |
皮肤病学 | 56篇 |
神经病学 | 257篇 |
特种医学 | 112篇 |
外科学 | 808篇 |
综合类 | 22篇 |
预防医学 | 76篇 |
眼科学 | 14篇 |
药学 | 271篇 |
中国医学 | 30篇 |
肿瘤学 | 371篇 |
出版年
2023年 | 25篇 |
2022年 | 30篇 |
2021年 | 94篇 |
2020年 | 67篇 |
2019年 | 72篇 |
2018年 | 88篇 |
2017年 | 67篇 |
2016年 | 62篇 |
2015年 | 72篇 |
2014年 | 107篇 |
2013年 | 122篇 |
2012年 | 223篇 |
2011年 | 227篇 |
2010年 | 108篇 |
2009年 | 96篇 |
2008年 | 208篇 |
2007年 | 184篇 |
2006年 | 191篇 |
2005年 | 230篇 |
2004年 | 208篇 |
2003年 | 179篇 |
2002年 | 185篇 |
2001年 | 72篇 |
2000年 | 88篇 |
1999年 | 85篇 |
1998年 | 67篇 |
1997年 | 51篇 |
1996年 | 41篇 |
1995年 | 43篇 |
1994年 | 35篇 |
1993年 | 35篇 |
1992年 | 62篇 |
1991年 | 59篇 |
1990年 | 61篇 |
1989年 | 54篇 |
1988年 | 45篇 |
1987年 | 48篇 |
1986年 | 37篇 |
1985年 | 42篇 |
1984年 | 32篇 |
1983年 | 32篇 |
1982年 | 17篇 |
1981年 | 12篇 |
1979年 | 19篇 |
1978年 | 20篇 |
1977年 | 19篇 |
1974年 | 14篇 |
1973年 | 13篇 |
1971年 | 16篇 |
1970年 | 19篇 |
排序方式: 共有4052条查询结果,搜索用时 15 毫秒
91.
Orii T Ohkohchi N Kikuchi H Sekiguchi S Kawagishi N Tsukamoto S Satomi S 《Hepato-gastroenterology》2003,50(50):530-531
A 76-year-old male patient with jaundice was diagnosed as having hepatic hilar cholangiocarcinoma. The patient underwent percutaneous transhepatic biliary drainage of the left and the right intrahepatic bile duct. He could not have a percutaneous transhepatic endoprosthesis placed because it was impossible to pass the guidewire through the stenotic portion of the lesion. The patient was operated, but the tumor was considered to be unresectable. Along the intrahepatic routes formed by the preexisting two percutaneous transhepatic biliary drainage tubes, silicon tubes were inserted. Through the lumen of a long jejunal limb, Roux-en-Y, the tubes with five to six side holes were passed in the distal direction and drawn out from the lumen of the jejunal limb and passed through the abdominal wall to outside. The tubes were occluded and buried in the subcutaneous space after a few clamping tests. He died of liver failure in his house four months after the operation without any symptoms of jaundice, fever or of obstruction of the tubes. 相似文献
92.
Morimasa Sekiguchi Yuzo Fujii Atsuko Saito Toshimitsu Suzuki Yasuko Shiroko Hisayoshi Nakamura Ken-ichiro Hasumi 《Journal of gastroenterology》1995,30(5):589-598
We describe a gastric carcinoma cell line that has been maintained in vitro for more than 10 years and retains the capacity
to produce a large amount of alpha-fetoprotein. This cell line was isolated from a metastatic lymph node of a 63-year-old
male patient with advanced gastric carcinoma (T2N3P0H0M0) who showed high serum levels of alpha-fetoprotein. The primary tumor
was moderately differentiated tubular adenocarcinoma and the lymph node was poorly differentiated adenocarcinoma without any
particular pattern. The cultured cells grew as densely packed islet-like colonies with small polygonal cells. Electron microscopy
revealed cells abundant in cytoplasmic organelles, with some cellular attachments being tight with junctional complexes and
some being loose across intercellular spaces. The free cell surface had microvilli. The population doubling-time was 152 h
at passage 58. Chromosomal analysis revealed the modal number to be 77, with numerous karyotype abnormalities. The tumorigenicity
of the cultured cells in athymic nude mice was positive only when they were subcutaneously transplanted beneath a plastic
plate, but when the cells were transplanted subcutaneously or administered by intrasplenic injection in intact or weakly irradiated
nude mice, no tumorigenicty was shown. The cell line produced tumor-associated antigens, such as alpha-fetoprotein, carcinoembryonic
antigen, and tissue polypeptide antigen. This cell line may be useful for comparative studies of different types of gastric
carcinoma and alpha-fetoproteins of different origins. 相似文献
93.
Shigemoto Yoko Matsuda Hiroshi Kimura Yukio Chiba Emiko Ohnishi Masahiro Nakaya Moto Maikusa Norihide Ogawa Masayo Mukai Yohei Takahashi Yuji Sako Kazuya Toyama Hiroshi Inui Yoshitaka Taki Yasuyuki Nagayama Hiroshi Ono Kenjiro Kono Atsushi Sekiguchi Kenji Hirano Shigeki Sato Noriko 《Annals of nuclear medicine》2022,36(5):460-467
Annals of Nuclear Medicine - Although previous studies have investigated age and gender effects on striatal subregional dopamine transporter (DaT) binding, these studies were mostly based on a... 相似文献
94.
Incidence of human parvovirus B19 DNA detection in blood donors 总被引:2,自引:0,他引:2
Yuko Yoto Tooru Kudoh Keiji Haseyama Nobuhiro Suzuki Takanori Oda Toshiaki Katoh Tsuneo Takahashi Sadami Sekiguchi Shunzo Chiba 《British journal of haematology》1995,91(4):1017-1018
Summary. 1000 serum samples from blood donors were tested for human parvovirus B19 (B19) DNA by a nested PCR assay: six samples were positive for B19 DNA. The frequency was 1/167 (0-6%), considerably higher than previous surveys (0-004-0-03%). Five of the six samples were also positive for anti-B19 IgM, indicating an acute phase of infection. It is recommended to screen for B19 DNA in blood products to prevent transfusion mediated viral infection for those susceptible such as immunocompromised patients and pregnant women. 相似文献
95.
Sakata Y Chancey AL Divakaran VG Sekiguchi K Sivasubramanian N Mann DL 《Basic research in cardiology》2008,103(1):60-68
The mechanisms that are responsible for the development of myocardial fibrosis in inflammatory cardiomyopathy are unknown.
We have previously generated lines of transgenic mice with cardiac-restricted overexpression of tumor necrosis factor (MHCsTNF
mice), a pro-inflammatory cytokine. The MHCsTNF mice develop a heart failure phenotype that is characterized by progressive
myocardial fibrosis, as well as increased levels transforming growth factor-β (TGF-β)(mRNA and protein. In order to determine
whether TGF-β-mediated signaling was responsible for the myocardial fibrosis observed in the MHCsTNF mice, we treated MHCsTNF
and littermate control mice from 4 to 12 weeks of age with a novel orally available TGF-β receptor antagonist (NP-40208).
At the time of terminal study, myocardial collagen content was determined using the picrosirius red technique, and left ventricular
(LV) systolic and diastolic function were determined using the Langendorff method. Treatment with NP-40208 resulted in a significant
(P < 0.05) 65% decrease in nuclear translocation of Smad 2/3, a significant (P < 0.05), decrease in the heart-weight to body-weight ratio from 6.5 to 5.7, a ∼37% decrease in fibrillar collagen content
(P < 0.01) and a significant (P < 0.05) decrease in the LV chamber stiffness by ∼25% in the MHCsTNF mice when compared to diluent-treated controls. Treatment
with NP-40208 had no discernable effect on LV systolic function, nor any effect on cardiac myocyte size or fetal gene expression
in the MHCsTNF mice. Taken together, these observations suggest that sustained pro-inflammatory signaling in the adult heart
is associated with a pro-fibrotic phenotype that arises, at least in part, from TGF-β-mediated signaling, with resultant activation
of Smad 2/3, leading to increased myocardial fibrosis and increased LV diastolic chamber stiffness.
Returned for 1. revision: 29 August 2007 1. Revision received: 24 September 2007 相似文献
96.
Masafumi Takahashi Hiromichi Sekiguchi Hideyuki Fujikawa Hideaki Mito Masato Eto Yukihiro Hojo Yoshizo Chiba Satoshi Kitamura Kazuyuki Shimada 《Catheterization and cardiovascular interventions》1994,31(4):290-292
Bilateral coronary artery fistula constitutes an uncommon subgroup of coronary artery fistulas that may have a distinct embryologic origin. Coronary artery fistulas usually show a tortuous arrangement upon coronary angiography, but aneurysmal dilatation is rare. We report here an extremely rare case of coronary artery fistula originating from both coronary arteries, which showed multicystic aneurysmal dilatation. 相似文献
97.
A case of neurofibroma located in the retroperitoneum involving the uncinate process of the pancreas
Hiromichi Imai M.D. Jun Kobayashi Ryoji Manabe Hiroshi Namio Tadashi Ichinona 《Journal of gastroenterology》1989,24(4):421-424
A case of solitary neurofibroma in the retroperitoneum involving the uncinate process of the pancreas, which has been reported
in only one case in Japan and less than 20 cases in the world literature, is described. A 57-year-old male complained of back
pain and microhematuria. Plain and enhanced abdominal computed tomography (CT) indicated an 8 × 6 cm hypovasular tumor located
in the uncinate process. We initially assumed it to be a cancer of the pancreas. His physical examination and blood chemistry
result were normal. Various diagnostic imaging methods however, disclosed a rare type of tumor. The tumor was resected by
pancreaticoduodenectomy and histological examination revealed it to be neurofibroma. 相似文献
98.
Yazawa T Uesaka M Inaoka Y Mizutani T Sekiguchi T Kajitani T Kitano T Umezawa A Miyamoto K 《Endocrinology》2008,149(4):1786-1792
We have shown previously that Cyp11b1, an 11beta-hydroxylase responsible for glucocorticoid biosynthesis in the adrenal gland, was induced by cAMP in androgen-producing Leydig-like cells derived from mesenchymal stem cells. We found that Cyp11b1 was induced in male Leydig cells, or female theca cells, when human chorionic gonadotropin was administered in immature mice. Expression of Cyp11b1 in rodent gonads caused the production of 11-ketotestosterone (11-KT), a major fish androgen, which induces male differentiation or spermatogenesis in fish. As in teleosts, plasma concentrations of 11-KT were elevated in human chorionic gonadotropin-treated mice. In contrast to teleosts, however, plasma concentrations of 11-KT were similar in both sexes, despite levels of testosterone, a precursor substrate, being about 20 times higher in male mice. Because expression of 11beta-hydroxysteroid dehydrogenase type 2, was much higher in the mouse ovary than in the testis, conversion of testosterone into 11-KT may occur more efficiently in the ovary. In a luciferase reporter system that was responsive to and activated by androgens, 11-KT efficiently activated mammalian androgen receptor-mediated transactivation. Our results suggest that the androgen metabolic pathway is conserved between teleosts and mammals, despite sexual dominance and reproductive functions of 11-KT being altered during evolution. 相似文献
99.
Yamauchi Y Aonuma K Takahashi A Sekiguchi Y Hachiya H Yokoyama Y Kumagai K Nogami A Iesaka Y Isobe M 《Journal of cardiovascular electrophysiology》2005,16(10):1041-1048
INTRODUCTION: Most idiopathic nonreentrant ventricular tachycardia (VT) and ventricular premature contractions (VPCs) arise from the right or left ventricular outflow tract (OT). However, some right ventricular (RV) VT/VPCs originate near the His-bundle region. The aim of this study was to investigate ECG characteristics of VT/VPCs originating near the His-bundle in comparison with right ventricular outflow tract (RVOT)-VT/VPCs. METHODS AND RESULTS: Ninety RV-VT/VPC patients underwent catheter mapping and radiofrequency ablation. ECG variables were compared between VT/VPCs originating from the RVOT and near the His-bundle. Ten patients had foci near the His-bundle (HIS group), with the His-bundle local ventricular electrogram preceding the QRS onset by 15-35 msec (mean: 22 msec) and His-bundle pacing produced a nearly identical ECG to clinical VT/VPCs. The HIS group R wave amplitude in the inferior leads (lead III: 1.0 +/- 0.6 mV) was significantly lower than that of the RVOT group (1.7 +/- 0.4 mV, P < 0.05). An R wave in aVL was present in 6 of 10 HIS group patients, while almost all RVOT group patients had a QS pattern in aVL. Lead I in HIS group exhibited significantly taller R wave amplitudes than RVOT group. HIS group QRS duration in the inferior leads was shorter than that of the RVOT group. Eight of 10 HIS group patients exhibited a QS pattern in lead V1 compared to 14 of 81 RVOT group patients. HIS group had larger R wave amplitudes in leads V5 and V6 than RVOT group. CONCLUSION: VT/VPCs originating near the His-bundle have distinctive ECG characteristics. Knowledge of the characteristic QRS morphology may facilitate catheter mapping and successful ablation. 相似文献
100.
Restoration of ultraviolet-induced unscheduled DNA synthesis of xeroderma pigmentosum cells by the concomitant treatment with bacteriophage T4 endonuclease V and HVJ (Sendai virus). 总被引:9,自引:7,他引:9
下载免费PDF全文
![点击此处可从《Proceedings of the National Academy of Sciences of the United States of America》网站下载免费的PDF全文](/ch/ext_images/free.gif)
K Tanaka M Sekiguchi Y Okada 《Proceedings of the National Academy of Sciences of the United States of America》1975,72(10):4071-4075
Ultraviolet (UV)-induced unscheduled DNA synthesis of xeroderma pigmentosum cells, belonging to complementation groups A, B, C, D, and E, was restored to the normal level by concomitant treatment of the cells with T4 endonuclease V and UV-inactivated HVJ (Sendai virus). The present results suggest that (1) T4 endonuclease molecules were inserted effectively into the cells by the interaction of HVJ with the cell membranes, (2) the enzyme was functional on human chromosomal DNA which had been damaged by UV irradiation in the viable cells, (3) all the studied groups of xeroderma pigmentosum ("variant" was not tested) were defective in the first step (incision) of excision repair. 相似文献