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991.
Shinya Yamasaki Yusuke Hashimoto Junsei Takigami Shozaburo Terai Masafumi Takahashi Shigeyuki Wakitani Hiroaki Nakamura 《Journal of tissue engineering and regenerative medicine》2017,11(3):609-617
The purpose of this study was to assess how peripheral blood cells (PBCs) contribute to meniscus repair, using a parabiotic rat model. Wild‐type (WT) and green fluorescent protein (GFP) transgenic rats were conjoined at the torso. After 4 weeks, the anterior part of the medial meniscus of both groups of rats was removed. At 1, 2, 4, 8 and 12 weeks post‐meniscectomy, repaired tissue was evaluated using stereomicroscopy, histology with toluidine blue staining, and immunofluorescence microscopy. Stereomicroscopic observations and confocal laser microscopy revealed that a high number of GFP‐positive cells were present in the repaired meniscus of WT rats 1 week post‐meniscectomy, and the number of GFP‐positive cells decreased over time. Based on blood chimerism, the ratios of PBCs in the repaired meniscus were 20.5 ± 2.3% at 1 week, 8.3 ± 0.9% at 2 weeks, 4.4 ± 0.9% at 4 weeks, 2.1 ± 0.9% at 8 weeks, and 0.5 ± 0.4% at 12 weeks, post‐meniscectomy. Histologically, fibrochondrocytes were observed in the repaired meniscus of WT rats after 4 weeks, some of which were GFP‐positive. The chondrogenic marker, type II collagen, was merged within the PBCs in the repaired tissue. However, type‐II‐collagen‐positive cell ratio and metachromasia in the repaired meniscus were not equivalent in normal meniscal tissue. This indicated that PBCs were present within the repaired meniscus at an early phase, replacing the excised meniscal cells, suggesting PBCs contributed to meniscal healing. The tissue repair contribution by these cells decreased at later phases. Copyright © 2014 John Wiley & Sons, Ltd. 相似文献
992.
Naoyasu Ueda Hiroaki Ida Masakazu Washio Hisaaki Miyahara Shoji Tokunaga Fumiko Tanaka Hiroki Takahashi Koichi Kusuhara Koichiro Ohmura Manabu Nakayama Osamu Ohara Ryuta Nishikomori Seiji Minota Shuji Takei Takao Fujii Yoshiaki Ishigatsubo Hiroshi Tsukamoto Tomoko Tahira Takahiko Horiuchi 《Arthritis \u0026amp; Rheumatology》2016,68(11):2760-2771
993.
Yusuke Shikama Yoshito Kamio Kazuyuki Kuriu Yasuhiro Shibuya Satoshi Kimura Hiroaki Nakajima 《Nihon Kokyūki Gakkai zasshi》2006,44(11):800-806
A 42-year-old woman presented with persistent cough, bloody sputum and fever. Her chest X-ray film showed an infiltrative shadow with cavitation in the upper lobe of the left lung. Acid-fast-bacilli were shown by sputum smear staining. The anti-tuberculosis drugs isoniazid, refampicin, ethambutol and pyrazinamide were prescribed, but her symptoms and chest X-ray findings did not improve. Findings of MTD and MAC-PCR were negative but Mycobacterium abscessus was confirmed by sputum culture with the DNA hybridization method. Combination therapy with clarithromycin, amikacin and imipenem/cilastatin for one month improved her symptoms and chest X-ray shadow, and clarithromycin monotherapy was carried out for another ten months. Drug susceptibility tests revealed this mycobacterium was sensitive to clarithromycin and amikacin. To determine the environmental factors related to this infection, several samples were examined. Acid-fast-bacilli were present in a smear from the bath room drain. However, to confirm the infectious routes, longer observation is needed. Moreover, serum amyloid protein A and ESR were useful markers to estimate the clinical course. 相似文献
994.
Yosuke Adachi M.D. Masaki Mori M.D. Munetomo Enjoji M.D. Hiroaki Ueo M.D. Keizo Sugimachi M.D. 《Diseases of the colon and rectum》1987,30(12):962-966
Eighteen cases of mesenteric panniculitis of the colon collected from the literature, together with two cases from the authors'
source, were reviewed. The disease occurred most often in late adult life, with a male predominance. Symptoms were abdominal
pain, diarrhea, constipation, and a lower abdominal mass in most patients. Barium enema disclosed narrowing, shortening, and
poor extensibility of the colon, and ultrasonography and computed tomography showed thickening of the mesocolon and colonic
wall with soft-tissue density. Exploratory laparotomy was done in all patients, and colectomy, colostomy, or other surgical
treatments were performed in 17 (85 percent). Gross appearance at the time of surgery was characterized by a marked thickening
or a firm mass of the mesocolon with a puckered surface involving the appendices epiploicae of the colon. Microscopically,
degeneration of the adipose tissue, revealed by aggregates of lipid-laden macrophages, was diagnostic. Inflammatory infiltration
and fibrosis also were present in many patients. Mesenteric panniculitis of the colon seems to be a lesion more advaced than
the same condition of the small intestine, and colostomy or bypass surgery may be needed for alleviation of severe symptoms. 相似文献
995.
Daiki Morimoto Tetsuya Tomita Shoko Kuroda Chikahisa Higuchi Shinichi Kato Toshikazu Shiba Hironori Nakagami Ryuichi Morishita Hideki Yoshikawa 《Journal of bone and mineral metabolism》2010,28(4):418-423
The existence of inorganic polyphosphates [poly(P)] in human cells has been demonstrated. In osteoblasts, it is suggested
that the concentration of cellular poly(P) is relatively high. In this study, we examined whether poly(P) accelerates the
differentiation of human mesenchymal stem cells (hMSCs) from patients with osteoarthritis (OA) and rheumatoid arthritis (RA)
into osteoblastic cells. Alkaline phosphatase (ALP) activity was induced by poly(P) in hMSCs from both OA and RA. In Alizarin
Red S and osteocalcin EIA, there was a significant difference between the control and poly(P) group. In real-time PCR, there
was a significant difference in ALP, collagen type 1A, osteocalcin, and bone sialoprotein between the control and poly(P)
group. Our findings suggest that poly(P) have the potent role of differentiating hMSCs into osteoblastic cells at the early
and later stages of osteoblastic differentiation. 相似文献
996.
Masahide Ikeguchi Tomoko Hatada Manabu Yamamoto Takanori Miyake Tomonori Matsunaga Kenji Fukuda Hiroaki Saito Shigeru Tatebe 《Surgery today》2010,40(3):228-233
Purpose
Patients diagnosed with early gastric cancer located in the middle third of the stomach have two major surgical options, namely a conventional distal gastrectomy with Billroth I anastomosis (DG) or a pylorus-preserving gastrectomy (PPG). Pyloruspreserving gastrectomy is thought to have greater functional benefits than DG, but the evaluation of its prognosis and outcome has so far been insufficient. 相似文献997.
Takiguchi N Nagata M Soda H Nomura Y Takayama W Yasutomi J Tohyama Y Ryu M 《Surgery today》2010,40(11):1050-1054
Purpose
We conducted this randomized trial to compare the LigaSure Vessel Sealing System with conventional methods in gastrointestinal carcinoma surgery at five specialty cancer hospitals. 相似文献998.
Tomoko Hatta Yasunari Fujinaga Masumi Kadoya Hitoshi Ueda Hiroaki Murayama Masahiro Kurozumi Kazuhiko Ueda Michiharu Komatsu Tadanobu Nagaya Satoru Joshita Ryo Kodama Eiji Tanaka Tsuyoshi Uehara Kenji Sano Naoki Tanaka 《Journal of gastroenterology》2010,45(12):1263-1271
Background
To assess the degree of hepatic fat content, simple and noninvasive methods with high objectivity and reproducibility are required. Magnetic resonance imaging (MRI) is one such candidate, although its accuracy remains unclear. We aimed to validate an MRI method for quantifying hepatic fat content by calibrating MRI reading with a phantom and comparing MRI measurements in human subjects with estimates of liver fat content in liver biopsy specimens.Methods
The MRI method was performed by a combination of MRI calibration using a phantom and double-echo chemical shift gradient-echo sequence (double-echo fast low-angle shot sequence) that has been widely used on a 1.5-T scanner. Liver fat content in patients with nonalcoholic fatty liver disease (NAFLD, n = 26) was derived from a calibration curve generated by scanning the phantom. Liver fat was also estimated by optical image analysis. The correlation between the MRI measurements and liver histology findings was examined prospectively.Results
Magnetic resonance imaging measurements showed a strong correlation with liver fat content estimated from the results of light microscopic examination (correlation coefficient 0.91, P < 0.001) regardless of the degree of hepatic steatosis. Moreover, the severity of lobular inflammation or fibrosis did not influence the MRI measurements.Conclusions
This MRI method is simple and noninvasive, has excellent ability to quantify hepatic fat content even in NAFLD patients with mild steatosis or advanced fibrosis, and can be performed easily without special devices. 相似文献999.
Hideki Nakamura Akitomo Okada Atsushi Kawakami Satoshi Yamasaki Hiroaki Ida Tomoko Masuda Taku Fukuda Katsuya Satoh Toshiro Yoshimura Munetoshi Nakashima Tomayoshi Hayashi Katsumi Eguchi 《Rheumatology international》2010,30(10):1381-1383
A 60-year-old man who had been diagnosed as rheumatoid arthritis admitted to our hospital by dysesthesia on his legs with edema. Nerve conduction velocity test led to diagnosis of mononeuritis multiplex. Magnetic resonance imaging (MRI) of lower legs showed high intensity in slow tau inversion recovery. Typical vasculitis with neutrophil-dominant cell infiltration was observed by muscle biopsy without inflammatory myopathy or fascitis. Diagnosis was made by rheumatoid vasculitis found in crural muscles. Intravenous cyclophosphamide with oral tacrolimus effectively improved dysesthesia with reduction of inflammatory response. 相似文献
1000.