全文获取类型
收费全文 | 4549篇 |
免费 | 258篇 |
国内免费 | 25篇 |
专业分类
耳鼻咽喉 | 34篇 |
儿科学 | 103篇 |
妇产科学 | 86篇 |
基础医学 | 595篇 |
口腔科学 | 126篇 |
临床医学 | 319篇 |
内科学 | 1215篇 |
皮肤病学 | 85篇 |
神经病学 | 251篇 |
特种医学 | 240篇 |
外科学 | 619篇 |
综合类 | 16篇 |
预防医学 | 86篇 |
眼科学 | 91篇 |
药学 | 310篇 |
中国医学 | 1篇 |
肿瘤学 | 655篇 |
出版年
2023年 | 33篇 |
2022年 | 40篇 |
2021年 | 111篇 |
2020年 | 48篇 |
2019年 | 83篇 |
2018年 | 110篇 |
2017年 | 97篇 |
2016年 | 103篇 |
2015年 | 74篇 |
2014年 | 127篇 |
2013年 | 163篇 |
2012年 | 241篇 |
2011年 | 284篇 |
2010年 | 153篇 |
2009年 | 122篇 |
2008年 | 211篇 |
2007年 | 262篇 |
2006年 | 271篇 |
2005年 | 265篇 |
2004年 | 258篇 |
2003年 | 240篇 |
2002年 | 241篇 |
2001年 | 107篇 |
2000年 | 130篇 |
1999年 | 107篇 |
1998年 | 65篇 |
1997年 | 43篇 |
1996年 | 37篇 |
1995年 | 34篇 |
1994年 | 35篇 |
1993年 | 29篇 |
1992年 | 70篇 |
1991年 | 76篇 |
1990年 | 71篇 |
1989年 | 79篇 |
1988年 | 44篇 |
1987年 | 38篇 |
1986年 | 52篇 |
1985年 | 40篇 |
1984年 | 32篇 |
1983年 | 30篇 |
1981年 | 17篇 |
1980年 | 15篇 |
1979年 | 23篇 |
1978年 | 9篇 |
1973年 | 9篇 |
1971年 | 8篇 |
1967年 | 10篇 |
1966年 | 8篇 |
1965年 | 8篇 |
排序方式: 共有4832条查询结果,搜索用时 468 毫秒
51.
The AMeX method. A simplified technique of tissue processing and paraffin embedding with improved preservation of antigens for immunostaining. 总被引:20,自引:7,他引:20
下载免费PDF全文
![点击此处可从《The American journal of pathology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Y. Sato K. Mukai S. Watanabe M. Goto Y. Shimosato 《The American journal of pathology》1986,125(3):431-435
A new simplified tissue processing method for immunostaining was devised. Tissues were fixed in acetone at -20 C overnight, then cleared in methyl benzoate and xylene, consecutively, and embedded in ordinary paraffin at 58-60 C. Thin paraffin sections were deparaffinized with xylene, immersed in acetone and then phosphate buffered saline, and immunostained with various monoclonal and conventional antibodies, which have only been used on fresh-frozen or PLP-fixed frozen sections. As with PLP-fixed frozen sections, the following antigens were clearly demonstrated in the tissue sections processed with the present method: T (Leu-1, Leu-2a, Leu-3a, Leu-4, OKT3, OKT4, OKT8), B (B1, B2, Leu-14, IgM, IgD) lymphocyte surface markers and other antigens (Leu-7, OKT6, OKT9, OKM1, OKI1, J5, Ki-1, Ki-67, TdT, oncogene Ha-ras P21). Sections prepared by the present method demonstrated much better histologic and cytologic preservation than possible in frozen sections. 相似文献
52.
Takahiro Hasebe Kiyoshi Mukai Kazuyuki Ishihara Akihiro Kaneko Yukio Shimosato 《Pathology international》1992,42(8):585-594
Thirteen sebaceous gland carcinomas and 10 sweat gland carcinomas were examined to elucidate any important histological parameters influencing their prognosis, and the relationship between immunohistochemical expressions of c-erbB-2 oncoprotein and survival of the patients was analyzed. Sebaceous gland carcinomas with vacuolated cytoplasm in more than 50% of whole tumor area, with necrosis, and without lymphoid cell infiltration in tumor nests and stroma had a higher incidence of tumor recurrence and tumor-related death than tumors with vacuolated cytoplasm in 50% or less of whole tumor area (p < 0.01), without necrosis, and with lymphoid cell infiltration in tumor nest and stroma (p < 0.05). Sweat gland carcinomas of all cases with fatal outcomes demonstrated tubular differentiation in 20% or less of whole tumor area, lymphatic permeation and desmoplastic reaction. Three sebaceous gland carcinomas and three sweat gland carcinomas were positive for c-erbB-2 oncoprotein. Two of three sebaceous gland carcinomas, and all three sweat gland carcinomas developed tumor recurrence and ended in tumor-related deaths. Sweat gland carcinomas with c erbB 2 expression had significantly shorter survival than those with negative immunostain (p < 0.01). Cytoplasmic appearance, tumor necrosis, and lymphoid cell infiltration in tumor nests and stroma of sebaceous gland carcinoma, and tubular differentiation, lymphatic permeation, and growth patterns of sweat gland carcinoma are considered to closely correlate to the prognosis. Immunohisto-chemically detected c erbB 2 oncoprotein may be an indicator of bad prognosis. Acta Pathol Jpn 42: 585–594, 1992. 相似文献
53.
54.
Masugi Y Kameyama K Aiba M Mukai M Hara S Ohigashi T Murai M 《Pathology international》2003,53(4):241-245
We report a case of non-functional adrenocortical adenoma of 5.5 x 5.5 x 3.2 cm in size that had an unusual histopathological appearance in two respects. First, the tumor contained small adipose foci with osteogenesis and was suspected of being a myelolipoma based on its appearance on computerized tomography (CT) and magnetic resonance imaging. However, pathologically, the fat element was seen focally and was not accompanied by hematopoietic cells, and the diagnosis of myelolipoma was abandoned. Second, the tumor was suspected of being an adrenal carcinoma based on its appearance on CT scans and showed extensive degeneration: fibrosis, hemorrhage, loss of parenchyma and moderate atypism of the tumor cells. However, as the architecture of the tumor cells was non-diffuse and there were no necrotic foci or mitoses, and vascular or capsular invasion were not present, the tumor was concluded to be an adrenocortical adenoma rather than a carcinoma. We diagnosed the tumor as a non-functional adrenocortical adenoma with extensive degeneration as the extensive areas of fibrosis were particularly remarkable. Furthermore, the extensive areas of degeneration might have been caused not only by an ischemic effect but also by low hormone levels. 相似文献
55.
56.
Interaction of rat ascites hepatoma cells with cultured mesothelial cell layers: a model for tumor invasion 总被引:13,自引:0,他引:13
H Akedo K Shinkai M Mukai Y Mori R Tateishi K Tanaka R Yamamoto T Morishita 《Cancer research》1986,46(5):2416-2422
Interactions of rat ascites hepatoma cells with primary cultured layers of rat mesentery-derived cells were studied. The mesentery-derived cells were isolated from rat mesentery and cultured in Eagle's minimum essential medium with a 2-fold concentration of amino acids and vitamins supplemented with 10% calf serum. The primary cultured cells, consisting mainly of mesothelial cells in polygonal shape, forms a "paving stone" sheet. Upon seeding the tumor cells on the mesentery-derived cell layers, three different types of tumor cell growth were observed. Type 1 was the formation of piled-up tumor cell nests on mesothelial cell layers. Type 2 was the formation of flattened tumor cell islands underneath mesothelial cell layers. This island formation was clearly observed under a phase contrast microscope 2 days after the tumor cell seeding. Protrusion of cellular processes of the tumor cells beneath mesothelial cells was occasionally seen. Type 3 was the growth of tumor cells in suspension. These types of tumor cell growth closely resemble those in the peritoneal cavity observed after i.p. implantation of the tumor cells. When the tumor cells recovered from the blood of tumor-bearing rats were seeded, flattened tumor cell islands were formed 15 times more frequently than when the tumor cells isolated from host peritoneal cavity were seeded. Shortly after the appearance of small flattened tumor cell islands, a distinct morphological change of mesothelial cells from polygonal to spindle shape was seen preferentially at the marginal area of the cell layers (a partial retraction of cell edges). The retraction of mesothelial cells was induced not only by seeding the tumor cells but by adding the tumor ascites fluid or the medium conditioned by the tumor cell culture. The morphological change was reversed by changing the culture medium to remove the effectors. These results indicate that the system described in this study can provide a useful model to study tumor cell invasion. 相似文献
57.
58.
59.
60.
Ishihara Hiroki Fukuda Hironori Tachibana Hidekazu Yoshida Kazuhiko Kobayashi Hirohito Takagi Toshio Iizuka Junpei Ishida Hideki Nagashima Yoji Kondo Tsunenori Tanabe Kazunari 《Clinical and experimental nephrology》2021,25(6):674-682
Clinical and Experimental Nephrology - The data regarding oncological outcome in advanced renal cell carcinoma (RCC) arising in end-stage renal disease (ESRD) are limited. Patients diagnosed with... 相似文献