针灸防治炎症性肠病的研究进展

炎症性肠病是一种全球性疾病,其机制尚未完全清楚,当前的治疗也存在一些不足。针灸作为一种绿色、安全、有效的治疗方式,对于炎症性肠病的防治具有一定的积极作用。本文通过对相关文献进行分析总结,发现目前针灸作为一种单独的治疗方式或同药物一起对炎症性肠病均具有一定的疗效,主要通过调节基因表达及表观遗传、提高机体免疫功能及调节炎症因子、改善肠道菌群、调节机体代谢物水平等途径延缓炎症性肠病的发展。现总结目前针灸防治炎症性肠病的研究进展,为进一步的研究及临床工作提供参考和依据。     

Pulse sequence extrapolation with MR image synthesis

Previous reports have presented validation studies of magnetic resonance (MR) image synthesis in which multiple spin-echo (MSE) source data were used to generate spin-echo images for various echo times and repetition times (TRs). A new method-"pulse sequence extrapolation" -synthesizes images for pulse sequences different from that of the acquisition. MSE data acquired in a time equivalent to a TR of 2,000 msec can be used to generate inversion-recovery (IR) images for arbitrarily chosen TI inversion times. Other combinations of pulse sequences were also studied, and synthetic images were compared visually and quantitatively to directly acquired images with corresponding parameters. Synthetic IR signals of the brain parenchyma consistently matched directly acquired signals to within 6%, with respect to the full magnetization signal. The noise level of synthetic signals was generally no more than twice that of direct acquisition signals, as predicted. This method can achieve selective fat suppression and enhancement in IR imaging.     

Membrane Characteristics and Synaptic Responsiveness of Superficial Dorsal Horn Neurons in a Slice Preparation of Adult Rat Spinal Cord

Intracellular recordings have been made from neurons of the superficial dorsal horn in slices of the lumbar and thoracic spinal cord of young adult rats. Three broad categories of neurons could be distinguished on the basis of their firing patterns to intracellular current pulses and their afterhyperpolarizations (AHP); there was no detectable difference in the regional distribution of the three types. Category 1 cells were characterized by maintained firing to intracellular depolarizing current pulses, brief action potential durations and polyphasic AHPs. Category 2 cells showed spike adaptation, without spike attenuation, during intracellular current pulses, and had monophasic AHPs. Category 3 cells fired only 1 or 2 spikes to maintained depolarizing pulses and had smaller monophasic AHPs than category 2 neurons. Spontaneous excitatory and inhibitory postsynaptic potential (epsp and ipsp) activity was seen with psp durations varying widely. Low intensity electrical stimulation of afferent fibres, or of superficial white matter, resulted in polyphasic epsps and/or ipsps. The spike discharge in response to such afferent inputs correlated with the membrane properties of the cells, such that the synaptic responses of category 1 neurons were usually bursts of spikes, whereas category 2 and 3 neurons either failed to fire or fired only a single spike. These results in adult rat spinal cord suggest that the discharge pattern within synaptic sensory responses of superficial dorsal horn neurons is determined by postsynaptic membrane properties as well as by the pattern of the afferent input.     

Assessing microleakage of different class V restorations after Er:YAG laser and bur preparation

This study assessed in vitro marginal leakage of class V cavities prepared by turbine and Er:YAG laser and restored with different materials. Sixty cavities with enamel and dentine margins were prepared and assigned to six groups: I, II, III by turbine and IV, V, VI by Er:YAG laser. The following restorative systems were used: groups I and IV: Bond 1 + Alert; II and V: Fuji II LC; III and VI: SBMP + Dispersalloy. After finishing, specimens were thermocycled for 8 h and 45 min (500 cycles), isolated, immersed in a 0.2% Rhodamine B solution, sectioned oro-facially and analysed for leakage. The dye penetration means (%) were: occlusal I: 10.09 (+/- 21.28), II: 3.25 (+/- 10.27), III: 0, IV: 41.77 (+/- 42.48), V: 23.37 (+/- 33.79), VI: 12.66 (+/- 24.06); cervical I: 16.49 (+/- 26.67), II: 4.34 (+/- 13.71), III: 0, IV: 37.71 (+/- 30.47), V: 39.56 (+/- 43.35) and VI: 72.53 (+/- 37.79). The use of Er:YAG laser for cavity preparation yielded higher degree of marginal leakage, as compared with the use of conventional air-turbine. The enamel interface provided better marginal sealing, comparing with dentine/cementum margin. As to the cavity preparation device (i.e. laser or bur), the analysis of the results showed that bonded amalgam and Fuji II LC provided less infiltration, than Alert. On the other hand, for lased cavities, Alert provided the best results, similar to those of Fuji II LC and superior to those reached by bonded amalgam.     

Prolonged opioid antagonism with naloxone in chronic renal failure.

Respiratory depression secondary to morphine intoxication occurred in an elderly patient with chronic renal failure (CRF). It was reversed with a continuous infusion of naloxone. Approximately 11 hours after the infusion was discontinued, the patient relapsed into respiratory depression consistent with opioid intoxication. He was rechallenged with a naloxone infusion with resolution of the opioid effects. This case suggests prolonged antagonism of opioid effects inconsistent with naloxone's reported pharmacologic effects. Serum naloxone concentrations measured after the end of the infusion suggest that the drug's pharmacokinetics were significantly altered. Further research is necessary to characterize pharmacokinetic changes that occur in CRF. In the absence of this information, similar patients should be closely monitored for relapse of respiratory depression after naloxone is discontinued.     

Acetylcholine-sensitive cells in the caudal medulla of the rat: distribution, pharmacology and effects of pentobarbitone.

1. The distribution of cholinoceptive and non-cholinoceptive cells in various nuclei of the caudal medulla of the rat is described. 2. The nature of the responses of cells of the paramedian reticular nucleus and of the perihypoglossal nuclei to electrophoretically applied acetylcholine (ACh) was investigated. 3. In unanaesthetized decerebrate preparations ACh responses were usually of a "fast onset-fast offset" nature. Dihydro-beta-erythroidine was a more effective antagonist than atropine. 4. In rats anaesthetized with barbiturate nearly all the tach responses showed a slower onset and prolonged action. Atropine was the more effective antagonist. 5. The synaptic responses of cells of the paramedian reticular and perihypoglossal nuclei to stimulation of glossopharyngeal, superior laryngeal, lingual and hypoglossal nerves were investigated. It is concluded that ACh does not mediate the responses at the level of these nuclei.     

Identification of Mycobacterium tuberculosis and M. avium complex directly from smear-positive sputum specimens and BACTEC 12B cultures by high-performance liquid chromatography with fluorescence detection and computer-driven pattern recognition models.

A high-performance liquid chromatography method that utilized fluorescence detection (HPLC-FL) of mycolic acid 6,7-dimethoxycoumarin esters was developed to identify Mycobacterium tuberculosis (MTB) and M. avium complex (MAC) directly from fluorochrome stain smear-positive sputum specimens and young BACTEC 12B cultures. HPLC-FL chromatograms from a training set that included 202 smear-positive clinical sputum specimens and 343 mycobacterial cultures were used to construct a calibrated peak-naming table and computer-based pattern recognition models for MTB and MAC. Pattern recognition model performance was measured with an evaluation set of samples that included 251 smear-positive clinical sputum specimens and 167 BACTEC 12B cultures. Evaluation sputum specimens were culture positive for MTB (n = 132) and MAC (n = 48). With evaluation sputa, the MTB and MAC models were 56.8 and 33.3% sensitive, respectively. Evaluation set BACTEC 12B cultures were culture positive for MTB (n = 97) and MAC (n = 53). The sensitivities of the MTB and MAC models for identification of BACTEC 12B cultures were 99.0 and 94.3%, respectively. The specificity of both models was 100% for both types of evaluation samples. The average times from BACTEC 12B inoculation to cell harvest were 10.2 and 7.4 days for MTB and MAC, respectively. HPLC-FL can identify MTB and MAC in 1 day from many smear-positive sputa. Rapid and sensitive identification of MTB and MAC from young BACTEC 12B cultures was achieved.     

Linkage of polymorphic congenital cataract to the gamma-crystallin gene locus on human chromosome 2q33-35

Cataract is one of the major causes of blindness in humans. We describe here an autosomal dominant polymorphic congenital cataract (PCC) which is characterised by wide variations in phenotype of non-nuclear lens opacities, even among affected members of the same family. PCC families included a large, unique pedigree (254 members, 103 affected individuals), and genetic linkage was conducted using a variety of polymorphic markers. Evidence for linkage was found for chromosome 2q33- 35 with PCC mapping near D2S72 and TNP1. A tri-nucleotide microsatellite marker for gamma-crystallin B gene (CRYG1) was found to co-segregate with PCC and yielded a maximum lod score of 10.62 at (theta = 0). A multipoint analysis demonstrated that the most probable location of the PCC gene was within an 8 cM genetic interval containing the gamma-crystallin gene cluster. These data provide strong evidence of the existence of an autosomal dominant mutation for PCC in or near the gamma-crystallin gene cluster. This defect is characterised by complete penetrance but variable expression of the cataract phenotype. Our study also suggests that non-nuclear human cataracts might be caused by some abnormality in gamma-crystallin genes.