DHS治疗股骨转子部骨折的临床应用体会

随着社会人口老龄化,股骨转子部骨折的发生率不断增高。虽然股骨转子部血运丰富,骨折容易愈合;但是如果处理不当,仍然可能预后功能不良。笔者回顾了我科自2001年8月至2004年5月期间应用动力髋螺钉(DHS)内固定治疗89例股骨转子部骨折,报道如下。临床资料本组89例,男33例,女56例;     

补肾方含药血清促进PC12细胞增殖及其拮抗谷氨酸神经毒作用的研究

目的运用中药血清药理学方法研究补肾复方含药血清(BS)促进体外培养的PC12细胞增殖及其拮抗谷氨酸兴奋性神经毒损伤的作用.方法以MTT法观察BS对体外培养的PC12细胞活力的影响及其拮抗谷氨酸损伤的作用,采用流式细胞仪观察BS对谷氨酸所诱导PC12细胞凋亡的影响.结果 BS含药血清可以增强PC12细胞活力,拮抗谷氨酸的兴奋性神经毒作用且在20%浓度时作用最强,并且能够抑制谷氨酸诱导的PC12细胞早期凋亡.结论补肾方BS可能具有神经保护作用.     

生化药物质量控制研究中应注意的问题

生化药物是从生物体分离、纯化所得的用于预防、治疗和诊断疾病的生化基本物质,以及用化学合成、微生物合成或现代生物技术制得的这类物质。生化药物有两个基本特点:其一,它来自生物体;其二,它是生物体中的基本生化成分。生化药物的生化基本物质主要包括氨基酸、肽、蛋白质、酶     

Novel estrogen receptor ligands and their structure–activity relationship evaluated by scintillation proximity assay for high‐throughput screening

The estrogen receptor (ER) is an important drug target with allosteric characteristics that binds orthotopic hormones and other ligands. A recently developed scintillation proximity (SPA)‐based assay for high‐throughput screening (HTS) of compound libraries was used to identify novel estrogen receptor ligands that might have ER subtype selective binding activity. Radioligand binding was determined in a multi‐detector scintillation counter designed for microtitration plates. Equilibrium binding experiments and kinetic competition tests were performed with [³H]‐estradiol and human ERα and ERβ receptors. A library of 6,000 structurally diverse compounds was screened. From this, several novel ligands were identified that showed pronounced subtype‐selective differences in ligand binding for ERα and ERβ. The observed equilibrium dissociation constant (K_d) for the binding of [³H]estradiol to ERα and ERβ receptors were approximately 0.25 and 0.64 nM, respectively. When 17β‐estradiol, raloxifene and daidzein were tested for binding affinity to ERα in a competition assay, the IC₅₀ values were 0.34, 1.31, and 75.6 nM, respectively. When tested for binding affinity to ERβ, the IC₅₀ values were 1.05, 11.4, and 10.6 nM, respectively. The results obtained show that the methodology is valid in comparison to previously published data regarding estradiol and other standard compounds (raloxifene and daidzein) binding characteristics of estrogen receptors. The assay is also well suited to applied research as a tool in HTS of compound libraries in the search of ER ligands. Several novel active compounds were identified and selected as potent ER subtype ligands. Drug Dev Res 64:203–212, 2005. © 2005 Wiley‐Liss, Inc.     

经方医学新论(续)

张仲景的《伤寒论》和《金匮要略》是经方医学的代表性著作，尽管其在中医学的发展历史中，不断地融入了医经医学的思想。前文认为，经方医学的科学性就在于经方医学严格地定义了每一个方剂。近年来，对汉代度量衡的研究取得了重要成果，许多学者对以容器计量和以个数计量的药物进行了实测，这些成果使我们能够对经方在古代的运用有了更明确的认识，也使我们能够准确地描述每一个经方。     

HPLC法同时测定地氯滴眼液中地塞米松磷酸钠及氯霉素含量

目的建立地氯滴眼液中主要成分地塞米松磷酸钠、氯霉素的高效液相色谱含量测定方法.方法色谱柱为Hypersil ODS C18(250 mm×4.6 m,5 μm),流动相为甲醇-0.34%的磷酸二氢钾水溶液(52:48,v/v),柱温25℃,检测波长240nm,内标:0.14 mg·mL-1氢化可的松.结果在0.1004～0.401 6 mg·mL-1范围内,地塞米松磷酸钠线性良好(r=0.999 98),回收率为102.5%,RSD=2.2%(n=9);在1.014 4～4.057 6 mg·mL-1范围内,氯霉素线性良好(r=0.999 98),回收率为101.8%,RSD=1.5%(n=9).结论该实验建立的分析方法灵敏、快速、准确.     

藤茶叶的扫描电镜鉴别研究

目的为准确鉴别药用藤茶的来源提供依据.方法对藤茶主要药用部位叶及叶柄进行了扫描电镜研究.结果叶上表面角质层厚、多非腺毛,下表面角质层纹理沿细胞壁方向延伸,叶横切面可见大型分泌腔,叶柄表面有稀疏非腺毛及细纵纹.结论电镜方法快速准确,尤以角质层纹理、表面非腺毛的鉴别特征特异性强.     

RP-HPLC测定祛疣软膏中盐酸小檗碱的含量

目的测定祛疣软膏中盐酸小檗碱含量.方法采用反相高效液相色谱法,Dikma HPLC column色谱柱(4.6 mm×200 mm,5 μm),以0.033 mol·L-1磷酸二氢钾溶液-乙腈(70∶30)为流动相,流速1.0 mL·min-1,检测波长349 nm,柱温25 ℃测定.结果RP-HPLC测定的线性范围为0.041～0.41 μg,相关系数为r=0.999 8;软膏中盐酸小檗碱的平均回收率为98.6%,RSD=1.6%(n=6),精密度良好(RSD=0.51%).结论该法操作简便、快速、准确,适用于祛疣软膏中盐酸小檗碱的含量测定.     

白斑颗粒的薄层色谱鉴别

目的:建立白斑颗粒的质量控制标准.方法:采用薄层色谱法对白斑颗粒中何首乌、丹参、桃仁、赤芍进行定性鉴别.结果:在薄层层析色谱中均能检出何首乌、丹参、桃仁和赤芍.结论:方法简便、专属性强,可用于该制剂的质量控制.     

Development of a dissolution medium for nimodipine tablets based on bioavailability evaluation.

A marked difference in the dissolution rate between two brands of nimodipine tablets was observed using a newly developed dissolution medium of pH 4.5 acetate buffer containing 0.05% sodium dodecyl sulfate (SDS). However, when pH 4.5 acetate buffer containing 0.3% SDS was used as dissolution medium, which was specified in the edition, the dissolution results of the both brands conformed to the BP requirements and no significant difference in dissolution was observed. The dissolution data obtained for two commercial brands of nimodipine tablets indicate the superiority of the proposed system as a discriminatory dissolution medium for nimodipine tablets. The relative bioavailability of the two brands of nimodipine tablets was determined in healthy adult volunteers after a single dose in a randomized crossover study. Plasma concentrations were determined by a liquid chromatography-tandem mass spectrometry method. Statistical comparison of the AUC(0-T), AUC(0- infinity), C(max), and T(max) indicated a significant difference in the two brands of nimodipine tablets.