Therapeutic effects of induced pluripotent stem cells in chimeric mice with β-thalassemia

Although β-thalassemia is one of the most common human genetic diseases, there is still no effective treatment other than bone marrow transplantation. Induced pluripotent stem cells have been considered good candidates for the future repair or replacement of malfunctioning organs. As a basis for developing transgenic induced pluripotent stem cell therapies for thalassemia, β⁶⁵⁴ induced pluripotent stem cells from a β⁶⁵⁴ -thalassemia mouse transduced with the normal human β-globin gene, and the induced pluripotent stem cells with an erythroid-expressing reporter GFP were used to produce chimeric mice. Using these chimera models, we investigated changes in various pathological indices including hematologic parameters and tissue pathology. Our data showed that when the chimerism of β⁶⁵⁴ induced pluripotent stem cells with the normal human β-globin gene in β⁶⁵⁴ mice is over 30%, the pathology of anemia appeared to be reversed, while chimerism ranging from 8% to 16% provided little improvement in the typical β-thalassemia phenotype. Effective alleviation of thalassemia-related phenotypes was observed when chimerism with the induced pluripotent stem cells owning the erythroid-expressing reporter GFP in β⁶⁵⁴ mouse was greater than 10%. Thus, 10% or more expression of the exogenous normal β-globin gene reduces the degree of anemia in our β-thalassemia mouse model, whereas treatment with β⁶⁵⁴ induced pluripotent stem cells which had the normal human β-globin gene had stable therapeutic effects but in a more dose-dependent manner.     

医院就诊患者肠道寄生虫感染状况调查

目的 了解医院就诊患者肠道寄生虫感染情况及临床特点,为健康状态的评估和采取有效防控措施提供依据.方法 对2010～2011年39 671例在广西医科大学第一附属医院就诊患者进行肠道寄生虫的病原学检查,并分析阳性患者的感染情况.结果 共查出感染者5 751例,总感染率为14.50％.检出8种寄生虫,感染率分别为华支睾吸虫5.96％、蛔虫0.45％、鞭虫0.24％、钩虫2.46％、溶组织内阿米巴0.82％、人芽囊原虫3.91％、蓝氏贾第鞭毛虫0.65％、人毛滴虫0.09％.男性感染率为16.78％,女性为10.77％,男性显著高于女性(P＜0.001); 30～岁组感染率最高,为24.19％;感染者大多有不良饮食习惯和或卫生习惯；感染者临床表现复杂多样、无特异性.结论 医院就诊患者土源性线虫感染率较低,但华支睾吸虫和人芽囊原虫感染率较高,是今后防治的重点,除早期诊断和药物治疗患者外,还应加强宣教工作力度,提高人群的疾病预防意识.     

病理学教学改革研究——以淋巴造血系统疾病为例

基础病理学教学中，淋巴造血系统疾病一直是难点，鉴于淋巴瘤发病率的逐年增高，教师对该章节的教学能力及教学质量也要更加重视。首都医科大学基础医学院病理学系以该教学难点为突破口，通过基础、临床的融合，整合及加强师资力量；集体备课提高年轻教师教学能力；收集及整理淋巴瘤的教学素材，包括典型病例、典型切片及分子诊断教学平台的搭建；积极开展课程思政融入教学等多种方式，同时多种教学方法联合应用，取得了很好的教学效果。并通过对以上工作及方法的总结，以期在未来的实验课教学、过程性考核、期末考核和线上虚拟教学平台中发挥重要作用，最终达到全面提高本科生基础病理学教学质量的目的。     

对我国多媒体健康教育项目发展的思考与启示

系统分析国外多媒体技术在病人健康教育中的应用研究,总结归纳多媒体项目设计特征与应用效果,以期为我国多媒体健康教育项目成功建设提供一定借鉴。     

Clinicopathological features and expression of four keratins (K10, K14, K17 and K19) in six cases of eruptive vellus hair cysts

Six cases of eruptive vellus hair cysts (EVHC) were evaluated for histopathology and the immunohistochemical profile of Ki‐67 and four keratins (K10, K14, K17 and K19). The pathological hallmark of EVHC was the existence of vellus hair shafts within the cystic cavity, but atypical pathological changes included two or three cysts and a foreign‐body granuloma in three cases. Our results demonstrate that atypical pathological changes are not uncommon in EVHC, and indicate that based on keratin expression, it is likely that EVHC is derived from the infrainfundibulum and sebaceous duct.