陶瓷内衬在金属髋臼中完全植入的研究

目的探讨陶瓷内衬在髋臼非完全性植入的发生率及分析其发生的因素。方法本研究对所有在两家本地医院使用Trident陶瓷内衬关节的患者进行回顾性研究。从2003年7月到2007年3月,共35例患者,平均年龄是50.2岁(29~65岁)。平均随访时间18.2个月(2~46个月)。用X线片评估髋臼内衬植入的完全性。结果本组有7例患者(20%)髋臼内衬的植入不完全,臼杯和内衬之间留有间隙。4例间隙的位置在外上方,另3例在内下方。所有患者在最后随访时,间隙都没有消失。结论 Trident髋臼的设计特点,令内衬在完全植入之前,容易被臼杯卡住。因为X线投照中心的不同,使这个错误的发生率和严重性被低估。     

Lamin C protein deficiency in the primary fibroblasts from a new laminopathy case with ovarian cystadenoma

Background Laminopathies are a group of rare genetic disorders characterized by multiple-tissue degeneration.We describe a new laminopathy with ovarian cystadenoma and explore its molecular etiology.Methods The case is a 15-year-old girl who presents the prominent progeroid disorders, multiple system degeneration and early-onset cystadenoma of the ovary.Candidate genes including LMNA, ZMPSTE24, PPAR G, INSR and WRN were sequenced to screen for DNA variants.The mRNA and protein expression levels of LMNA were examined in primary fibroblasts.The pathophysiological events such as morphologic alterations, cell senescence, cell proliferation,apoptosis and pRb as well as p53 protein expressions were also investigated in primary fibroblasts.Results No mutation was identified in the candidate genes screened.Nuclear abnormalities including nuclear blebs,mislocalization of lamin A/C were evident in the patient fibroblasts.Ultrastructurally, nucleus exhibited nuclear herniation and almost complete loss of peripheral heterochromatin.In addition, lamin C protein expression was markedly reduced whereas lamin A protein level was normal and no prelamin A was detected in the primary fibroblasts.Although the senescence-associated β-galactosidase staining of patient＇ cells was negative, cells in S phase increased in accompany with a decrease in pRb protein expression.Furthermore, increases in apoptotic cell death and p53 expression were observed.Conclusions Our data suggest that selective deficiency of lamin C protein is associated with a case of laminopathy with ovarian cystadenoma.The abnormalities in nuclear structure and alterations in gene expression such as the decrease in pRb and increase in p53 may be responsible for the multiple tissue degeneration.     

膀胱灌注治疗宫颈癌根治术后尿潴留疗效观察

目的了解膀胱灌注治疗宫颈癌术后尿潴留的效果。方法60例首次行广泛性全子宫切除术＋盆腔淋巴结清扫术的宫颈癌患者随机分为观察组（n=30）和对照组（n=30）,两组均采取综合治疗护理措施,对照组采用传统的方法拔除尿管后测残余尿量,若残余尿量〉100ml予重新留置尿管3d。观察组在综合治疗护理措施的基础上加用膀胱灌注保留膀胱灌洗液,拔除尿管后不再留置导尿管。结果观察组有3例发生尿潴留,对照组有10例发生尿潴留,两组差异有统计学意义（P〈0．05）。结论综合护理配合膀胱灌注保留膀胱灌洗液对治疗宫颈癌术后尿潴留有效。     

复方鳖甲软肝方防治肺纤维化大鼠自由基损伤作用的实验研究

目的研究复方鳖甲软肝方防治肺纤维化大鼠自由基损伤的作用和机制。方法SD雄性大鼠随机分为正常组、假手术组、模型组、强的松(0.56 m g/kg)组、复方鳖甲软肝方高、中、低剂量(1.4、0.7、0.35 g/kg)组,每组10只。除正常组、假手术组外,其余各组大鼠气管滴注盐酸博莱霉素制备大鼠肺纤维化模型。造模后第2天,各组分别ig给药,连续给药4周。测定各组大鼠肺组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(G SH-Px)、诱导型一氧化氮合酶(iNO S)活性和丙二醛(M DA)水平;采用W estern b lotting实验研究肺组织蛋白中核因子κB(NF-κB)和核因子κB抑制剂(IκBα)的表达。结果复方鳖甲软肝方能提高肺纤维化大鼠体内的G SP-Px和SOD活性,降低M DA水平和iNO S活性,减弱肺纤维化大鼠肺组织核蛋白中NF-κB表达,增强胞浆蛋白中IκBα的表达。结论复方鳖甲软肝方能调节肺纤维化大鼠体内自由基水平,减轻自由基对肺组织结构的氧化损伤,调节肺组织蛋白IκBα的表达,进而抑制NF-κB表达,是该方防治肺纤维化的作用机制。     

肝十二指肠韧带内注射利多卡因对缺血-再灌注损伤肝脏的影响

目的探讨肝十二指肠韧带内注射利多卡因减轻肝脏缺血-再灌注(Ischemia-reprefusion,I-R)损伤的有效性以及从蛋白质组水平研究其可能涉及的机制. 方法 46 只 SD 大鼠随机分成 I-R 组、利多卡因组和开腹组.检测各组血清丙氨酸转氨酶(ALT)和门冬氨酸转氨酶(AST),肝脏组织标本胎盘蓝染色分析.肝脏组织的蛋白质组学分析采用双向聚丙烯酰胺凝胶电泳(2-D PAGE)、质谱( MALDI-TOF-MS)方法. 结果 利多卡因组 ALT 和 AST 较 I-R 组显著降低(ALTt=2.599,P<0.02;ASTt=2.992,P<0.01),胎盘蓝染色的无活性肝细胞较I-R组也明显减少.利多卡因组的 2-DPAGE 图谱较I-R 组发生了改变,质谱鉴定出 11 个差异表达的蛋白质,其中 6 个上调表达,5 个下调表达. 结论肝十二指肠韧带内注射利多卡因预处理有效地减轻了大鼠肝脏随后的 I-R 损伤,是一种保护面临 I-R 损伤肝脏的新方法.蛋白质组学研究可见,利多卡因预处理改变了肝脏 I-R 后的蛋白质表达.     

国产安息香的研究

1960—1962年间,我們在广西、云南地区发現了三种安息香属植物:粉背安息香(Styrax hypoglaucus Perk.)、青山安息香(S. macrothyrsus Perk.)及白叶安息香(S.subniveus Merr.et Chun)。对这些植物进行了形态鉴定及生长环境的观察,并将所得树脂进行了化学定性鉴别及醇不溶物、总香脂酸、游离香脂酸及总香脂酸中苯甲酸的含量測定。根据三种树脂的定性及定量分析結果,认为可以考虑作为药用安息香的新資源,进一步研究其生产与利用。     

老鸭嘴化学成分研究

目的:对老鸭嘴乙醇提取物的活性部位进行化学成分研究.方法:对老鸭嘴提取液不同极性部位进行醛糖还原酶抑制活性筛选,活性部位应用常规柱色谱及制备高效液相手段相结合进行化学成分分离,采用波谱技术对所得到的化合物进行结构鉴定.结果:从乙酸乙酯部位中分离鉴定了8个化合物,分别为新化合物8-羟基-8-甲基-9-亚甲基-环戊烷[7,11]-1,4,6-三羟基-四氢化萘-12-酮,命名为山牵牛素A(1),3,4-二氢4,5,8-三羟基-2-(3-甲基-2-丁烯基)萘[2,3-b]环氧乙烯-1(2H)-酮(2),8-(β-葡萄糖吡喃型-O-)-3,4-二氢-2-(3-甲基-2-丁烯基)萘[2,3-b]环氧乙烯-1(2H)-酮(3),高良姜素(4),槲皮素(5),木犀草素(6),5,6,3’,4’-四羟基-3,7-二甲氧基黄酮(7)和羽扇豆醇(8).结论:老鸭嘴乙酸乙酯提取物有明显的抑制醛糖还原酶活性,从该提取物中分离得到1个新的四氢化萘衍生物山牵牛素A,首次从该属植物中分离得到环氧萘类化合物及其苷,还有4个黄酮类化合物,1个三萜化合物.     

头晕的心理因素研究

目的探讨头晕患者的相关心理因素。方法对90例头晕患者和50名正常人进行明尼苏达多相人格调查表(MMPI)、汉米尔顿抑郁量表(HAMD)、汉米尔顿焦虑量表(HAMA)、多伦多述情障碍量表(TAS)评定。将患者随机分为常规治疗组和合用帕罗西汀组,治疗2月后(两组失访4例和6例)观察患者HAMD、HAMA量表评分的变化及其与头晕症状变化的关系。结果治疗前,与正常对照组比较,患者组TAS、HAMD、HAMA总分高,MMPI提示人格有偏移。治疗后,合用帕罗西汀组的HAMD分、HAMA分及头晕症状的改善均优于常规组。结论头晕患者的人格因素和述情方式有明显异常,存在抑郁焦虑等负性情绪。常规治疗加帕罗西汀可改善患者的抑郁焦虑症状,从而有助于头晕症状的缓解。     

Association between shift rotation and 30-year Framingham risk of cardiovascular disease among male workers in a medium-sized manufacturing factory

Rotating shift work is associated with an increased risk of cardiovascular disease (CVD). This study compared the CVD risk score in 129 male line workers aged 22–49 years on different shifts in a medium-sized metal production factory from 2017 to 2020. We classified workers into four groups: permanent day shift, weekly rotation involving five consecutive nights, weekly rotation involving 3–4 consecutive nights, and monthly rotation involving two consecutive nights. We used the Framingham Risk Score to estimate the 30-yr risks of general and hard CVD (CVD risk estimates). We investigated the differences in CVD risk estimates between different groups using linear mixed models. The average 30-yr Framingham CVD risk estimates of each group ranged from 17.5% to 31.2% for general CVD and from 10.5% to 20.5% for hard CVD. Workers on weekly rotations involving 3–5 consecutive nights had 5%–10% significantly higher CVD risk estimates than workers on the permanent day shift. Workers on weekly rotations also had 6%–8% higher BMI-based CVD risk estimates than those on the monthly rotation involving two consecutive nights. While 24-h shift rotations are unavoidable, our findings underscored the potential CVD risk among workers on weekly rotations involving more consecutive nights.     

无张力疝修补术在成人腹股沟复发疝中的应用

目的探讨无张力疝修补术在成人腹股沟复发疝中的临床应用价值。方法采用人工合成材料行成人腹股沟复发疝无张力疝修补术56例,59侧。结果手术全部成功,随访时间4~89个月,无严重术后并发症,有2例复发,总体效果良好。结论无张力疝修补术安全、操作简单,创伤小,并发症少,术后恢复快,复发率低,是治疗成人复发性腹股沟疝患者的理想术式。