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21.
高能量损伤导致的下肢严重开放性损伤的保肢治疗,对骨科医生来说是一个挑战。随着修复重建技术的提高和术后辅助治疗的完善,给保肢治疗带来了希望,但总体疗效仍不理想。我院自2003年1月~2004年12月共对32例下肢严重损伤患者行保肢治疗,现结合相关文献进行回顾性分析,旨在探讨保肢的指征、肢体重建的方法与时机及保肢失败的原因。 相似文献
22.
目的 :讨论肝胆管结石合并胆管癌的临床特点和诊治方法。方法 :对 11例肝胆管结石合并胆管癌的临床资料进行回顾性研究。结果 :本组肝胆管结石合并胆管癌的术前确诊率为 18.2 % (2 /11) ;术中冰冻病理检查确诊 2例 ;术后病理证实 7例。肝门胆管癌 72 .7% (8/11) ,中段胆管癌 9.1% (1/11) ,下段胆管癌 18.2 % (2 /11)。肿瘤根治切除率 36 .4 % (4/11) ,姑息性手术 6 3.6 % (7/11)。结论 :提高对本病的认识及详细的术中探查是及早发现胆管癌的重要途径。胆管癌根治性切除明显优于姑息性手术。 相似文献
23.
瘢痕疙瘩是烧伤外科研究的重要课题,其形成机制尚未完全明确,本文就其在遗传机制、免疫作用、细胞增殖与胶原代谢、细胞因子等方面的研究进展进行综述。 相似文献
24.
目的探讨碱性成纤维细胞生长因子(bFGF)不同剂量、用药方法对扩张组织的影响.方法以白色家猪为实验动物,采用持续恒压扩张技术,观察不同剂量和不同给药时间,bFGF和硫糖铝对扩张组织的影响及组织结构变化.结果扩张术同时每日2次局部应用bFGF 9AU/cm2 硫糖铝100μg/ml,共7 d,浸于明胶海绵上缓慢持续释放对扩张组织的影响最明显,效果最佳,扩张器内实际注液量、扩张所获得的皮肤净增面积明显增加,皮瓣回缩率降低.表皮细胞层数增多,胶原纤维、弹力纤维、成纤维细胞密度和毛细血管密度显著增高.药量增加效果未随之增加.结论持续恒压扩张术时合用bF-GF 9AU/cm2 硫糖铝100μg/ml,每日2次注药浸入明胶海绵持续缓慢释放效果最佳. 相似文献
25.
目的 探讨在ELISA检测HBsAg试验中,标本混入WBC后,WBC的粘附现象对试验结果的干扰。方法 观察WBC粘附现象对测定结果的干扰情况,以及血浆中WBC含量与干扰程度的关系,同时采用增加洗涤次数的方法对抗WBC粘附的效果观察。结果 标本混入WBC,WBC在反应板内发生粘附。WBC的中性粒细胞含过氧化物酶对试验结果造成干扰;标本中单位体积WBC含量与WBC的粘附量不成正比,但WBC的有效粘附数与OD值直接相关,粘附量越多,OD值越高;且WBC的粘附经洗涤10次也未能完全洗脱。结论 在ELISA检测HBsAg工作中应注意WBC对试验产生的非特异性干扰,采用多种方法防止WBC的混入。 相似文献
26.
Venous and arterial thrombosis are closely related to many severe diseases, especially to cardiovascular and cerebrovasular disorders. Thrombolytic therapy has been proven to be an effective method to treat such disease, which decreased the mortality and morbidity greatly. 相似文献
27.
Objective To observe the effect of intrathecal clonidine plus morphine on expression of protein kinase A (PKA) catalytic subunit in the spinal dorsal horn in a rat model of incisional pain. Methods Eighty male Sprague-Dawley rats were divided randomly into five groups: sham group, control group, pre-incisional morphine 2.5 μg group, pro-incisional clonidine 5 μg group and preincisional morphine 2.5 μg plus clonidine 5 lag group (n=16). lntrathecal catheter and the model of incisional pain were pro-duced according to Yaksh and Brennan's described method respectively. Changes of pain behavior were assessed by mechanical with-drawal threshold (MWT) and thermal withdrawal latency(TWL). The expressions of PKA catalytic subunit in the spinal dorsal horn were assessed by immunohistochemical method and western blotting analysis. Results Compared with sham group, MWT and TWL in control group were decreased significantly at 2 h after incision (P<0.01) and the number of positive cells and protein expression of PKA catalytic subunit in the spinal dorsal horn were increased significantly in control group (P<0.01). Compared with control group, MWT and TWL in pre-incision morphine 2 μg plus clonidine 5 lag group were increased significantly at 2 h after incision (P<0.01) and the number of positive cells and protein expression of PKA catalytic subunit in the spinal dorsal horn were decreased significantly in pre-incision morphine 2 μg plus clonidine 5 μg group (P<0.01). However, MWT, TWL and the number of positive cells and pro-tein expression of PKA catalytic subunit in the spinal dorsal horn changed with no statistical significance in pre-incisional morphine 2.5 μg group and pre-incisional clonidine 5 μg group compared with control group. Conclusion lntrathecal clonidine significantly enhances the antinociceptive effect of intrathecal morphine in a rat model of incisional pain, which might be associated with inhibi-tion of the increased expression of PKA catalytic subunit in spinal cord. 相似文献
28.
目的利用MR显微成像技术研究阿尔茨海默病转基因小鼠老年斑的沉积情况。方法2只17个月龄阿尔茨海默病转基因[V717I]小鼠和2只野生型小鼠,行活体T2WI,然后对照影像定位结果进行组织学切片及免疫组织化学染色。观察T2WI中老年斑的沉积情况以及其与免疫组织化学染色结果的对应关系。结果转基因小鼠T2WI上显示大脑皮层和海马区可见点状低信号,且部分低信号可以和组织学切片所显示的老年斑相对应;野生型小鼠T2WI未见明显的低信号,免疫组织化学染色也未见到染色阳性的斑块。结论MR显微成像技术检测老年斑的沉积是可行的,且可用来特异性地诊断阿尔茨海默病。 相似文献
29.
Objective To elucidate the effect of FasL gene expression on the proliferation and apoptosis of hypoxic rectal carcinoma cells. Methods The normoxic expression level of FasL in HR-8348 subtype cells (HR-8348B, HR-8348L, HR-8348F and HR-8348As) with different invasive power were verified by Western blot. Hypoxia models for HR-8348B, HR-8348L, HR-8348F and HR-8348As were constructed with chemical modeling, then the FasL levels in all groups at 12 h after hypoxia were quantitated by Western blot. Distribution of different cell life cycles was determined with flow cytometry. Cell reproductive activities were detected with MTT method, and cell apoptesis was assessed with TUNEL. Results FasL protein was pigmentized at the position of 40 000 by Western blot, and the expression level of FasL was significantly higher in HR-8348F cells than those in HR-8348B, HR-8348L and HR-8348As cells(F=361.149, P<0.01) in normoxia. At 12 h after hypoxia, the FasL level was also significantly higher in HR-8348F cells than those in other groups (F=278.766, P<0.01), but was not markedly different as compared to themselves in normoxia (t=1.762, P>0.05). The proliferation index was significantly higher in HR-8348F (60.43±3.72) than those in HR-8348B (40.01±3.30), HR-8348L (41.30±4.06) and HR-8348As cells (35.87±4.39), respectively (F=39.477, P<0.01). However, both inhibition rate of proliferation and apoptotic index were remarkably lower in HR-8348F (17.30±1.98 and 13.10±1.04) than those in HR-834B (33.70±4.33 and 21.60±1.31), HR-8348L (34.20±3.92 and 20.10±1.15), and HR-8348As (38.00±4.55 and 23.90±1.23), respectively (F=28.811 and 76.462, respectively, P<0.01). Conclusion The expression enhancement of intracellular FasL in rectal carcinoma in hypoxia can lead to accelerated proliferation and reduced apeptosis of cells, which will promote tumor cells to adapt microenvironmental hypoxia. 相似文献
30.
对宿主易感性的认识使人们能够理解不同个体牙周炎发生、发展的差异,而另一方面,牙周疾病对许多器官系统的潜在影响日益受到关注,牙周医学的兴起开创了崭新的重要研究领域,并有着深远的临床意义。 相似文献