全文获取类型
收费全文 | 319篇 |
免费 | 18篇 |
国内免费 | 32篇 |
专业分类
耳鼻咽喉 | 2篇 |
儿科学 | 25篇 |
妇产科学 | 4篇 |
基础医学 | 31篇 |
口腔科学 | 8篇 |
临床医学 | 61篇 |
内科学 | 71篇 |
皮肤病学 | 19篇 |
神经病学 | 6篇 |
特种医学 | 58篇 |
外科学 | 12篇 |
综合类 | 13篇 |
预防医学 | 3篇 |
眼科学 | 8篇 |
药学 | 27篇 |
中国医学 | 2篇 |
肿瘤学 | 19篇 |
出版年
2022年 | 4篇 |
2020年 | 2篇 |
2019年 | 1篇 |
2018年 | 6篇 |
2017年 | 3篇 |
2016年 | 8篇 |
2015年 | 6篇 |
2014年 | 8篇 |
2013年 | 10篇 |
2012年 | 7篇 |
2011年 | 8篇 |
2010年 | 11篇 |
2009年 | 22篇 |
2008年 | 9篇 |
2007年 | 33篇 |
2006年 | 12篇 |
2005年 | 1篇 |
2004年 | 9篇 |
2003年 | 6篇 |
2002年 | 7篇 |
2001年 | 9篇 |
2000年 | 2篇 |
1999年 | 5篇 |
1998年 | 16篇 |
1997年 | 19篇 |
1996年 | 24篇 |
1995年 | 12篇 |
1994年 | 23篇 |
1993年 | 5篇 |
1992年 | 2篇 |
1991年 | 6篇 |
1990年 | 7篇 |
1989年 | 9篇 |
1988年 | 7篇 |
1987年 | 5篇 |
1986年 | 8篇 |
1985年 | 5篇 |
1984年 | 4篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1980年 | 2篇 |
1979年 | 2篇 |
1978年 | 4篇 |
1977年 | 12篇 |
1976年 | 2篇 |
1975年 | 1篇 |
1970年 | 1篇 |
排序方式: 共有369条查询结果,搜索用时 15 毫秒
91.
HC Morton P Hilton 《BJOG : an international journal of obstetrics and gynaecology》2009,116(8):1120-1126
The introduction of the TVT™ has brought about dramatic changes in our practice as regards surgery for stress urinary incontinence, although the risk of bladder injury has caused concern. The subsequent development of tapes introduced through the obturator foramina may limit the rate of bladder injury, and some have suggested that cystoscopy is no longer required during stress incontinence surgery. Several cases of urethral injury referred to our unit have caused us concern about this approach. We present here brief details of these cases along with a systematic search of the literature on urethral injury following mid-urethral tape procedures. 相似文献
92.
O'Brien MF Hegarty PK Healy C DeFrietas D Bredin HC 《Irish journal of medical science》2004,173(1):18-19
Background The Fowler-Stephens orchidopexy (FSO) is a well-described treatment for high maldescended testes where the limiting factor
for successful placement in the scrotum is short testicular vessels. The operation involves division of these vessels. The
testicular blood supply is then dependent on collaterals from the vasal artery.
Aims To assess the long-term outcome of patients who underwent this procedure in our institution.
Methods The medical records of 20 patients who underwent 22 FSO from 1978 to 1999 by one urologist (HB) were reviewed. Outcome was
assessed in terms of testicular position and size.
Results Age at operation ranged from 2 to 14 years (mean 5.8 years). All patients had a one-stage FSO and in two of them the procedure
was bilateral. In five patients, FSO was preceded by a diagnostic laparoscopy. Mean follow up was 22 months (range 0–121 months).
Overall, results were considered good in 18 of 22 testes (82%).
Conclusion Our results for the one-stage FSO are comparable with other procedures for the management of high maldescended testis. 相似文献
93.
Oxaliplatin is a platinum derivative with an overall excellent safety profile that has a major role in the treatment of colorectal cancer. With a proven role now in the adjuvant setting, rare but potentially life‐threatening toxicities become a more significant issue. We report here a case of significant postinfusion hemolysis and thrombocytopenia in a patient undergoing adjuvant chemotherapy for stage III colon cancer, and review the literature. Six cases of hemolysis following oxaliplatin treatment have previously been reported, all in the setting of advanced colorectal cancer, with one case resulting in death. In three of the seven reports (including the present case), warning signs of low grade hemolysis were apparent during preceding cycles, with fever and/or back pain during the infusion being the most common feature. Our case appears to be the first reported with a direct antiglobulin test‐negative hemolysis with thrombocytopenia, with each of the previous reports postulating an autoimmune basis. Hemolysis and/or thrombocytopenia are potentially life‐threatening complications of oxaliplatin chemotherapy. With the increasing use of oxaliplatin in the adjuvant setting, clinicians need to be aware of this entity and the possible clinical warning signs that may be evident in preceding cycles. 相似文献
94.
Studies of the multistage nature of hepatocarcinogenesis in the rat have
led to the development of models having significant potential application
to carcinogenesis in other tissues as well as other species. Whereas the
initial and final stages of carcinogenesis- initiation and
progression-involve genetic changes and are operationally irreversible, the
intermediate stage of promotion is operationally reversible and can be
modulated by a variety of environmental factors. Numerous investigations
have demonstrated that chronic caloric restriction modifies neoplastic
development, primarily during the stage of promotion, so that fewer lesions
develop. Short- term fasting of rats, initiated with a nonnecrogenic dose
of diethylnitrosamine (DEN) and promoted with 0.05% phenobarbital (PB) for
4 weeks, results in loss of virtually all of the measurable altered hepatic
foci (AHF) after two 5-day periods of fasting with an intermediate 2-day
period of feeding. This change was accompanied by a marked decrease in
bromodeoxyuridine (BrdU) labeling of hepatocytes within AHF together with a
significant increase in apoptosis of such cells measured by nick
end-labeling. Similar but lesser effects were noted in surrounding,
nonfocal hepatocytes. On refeeding, both the numbers and volume percentage
of AHF returned within 2 weeks to values seen in nonfasted controls.
Administration of PB during the fasting period did not alter these results,
although AHF reappeared more rapidly in such animals on refeeding. Nuclear
DNA fragmentation was evident in samples of whole liver from fasted
animals. During this same period the expression of c-myc mRNA increased 3-
to 9-fold, while levels of albumin and insulin-like growth factor I mRNAs
decreased significantly. This study demonstrates a model system in which
the reversibility of the effects of promoting agents may be rapidly
determined and the effects of chemopreventive inhibitors of promotion may
be rapidly evaluated.
相似文献
95.
Methyl-hydroxylated metabolites of the potent carcinogen, 7,12-
dimethylbenz[a]anthracene (DMBA), namely, 7-hydroxymethyl-12-
methylbenz[a]anthracene (7-OH-DMBA), 7-methyl-12-
hydroxymethylbenz[a]anthracene (12-OH-DMBA) and 7,12-
dihydroxymethylbenz[a]anthracene (7,12-diOH-DMBA), were examined as
substrates for sulfotransferase bioactivation in different human tissue
cytosols. Hepatic cytosols, which were able to catalyze the 3'-
phosphoadenosine 5'-phosphosulfate (PAPS)-dependent DNA binding of 7-OH-
DMBA, 12-OH-DMBA and 7,12-diOH-DMBA, were highly sensitive to inhibition by
dehydroepiandrosterone (DHEA), a specific substrate for human DHEA-steroid
sulfotransferase (IC50 = 5 microM). By comparison,
2,6-dichloro-4-nitrophenol, a potent inhibitor of the thermostable (TS)-
phenol and estrogen sulfotransferases, did not have an appreciable
inhibitory effect. Neither p-nitrophenol, a high affinity substrate for
human TS-phenol and estrogen sulfotransferases, nor dopamine, a specific
substrate for the thermolabile (TL)-phenol sulfotransferase, significantly
inhibited the DNA binding of 12-OH-DMBA catalyzed by hepatic cytosols.
Inter-subject variation (n = 12) of the PAPS- dependent DNA binding of
12-OH- and 7,12-diOH-DMBAs also correlated well with DHEA-sulfotransferase
activity (r = 0.90; P < 0.00001 and r = 0.92; P < 0.00001,
respectively). This sulfation-dependent metabolic activation was not
detected in cytosols from human colon, pancreas, larynx or mammary gland.
Both TS- and TL-phenol sulfotransferases were active in human liver and
colon but only liver contained DHEA- sulfotransferase activity. These
results indicate that the sulfotransferase-mediated activation of the
methyl-hydroxylated DMBAs is predominantly catalyzed by DHEA-steroid
sulfotransferase in human liver and that TS- and TL-phenol
sulfotransferases and estrogen sulfotransferase are not involved in the
catalysis.
相似文献
96.
97.
Monoclonal antibodies to mammalian heat shock proteins impair mouse embryo development in vitro 总被引:7,自引:0,他引:7
Neuer A; Mele C; Liu HC; Rosenwaks Z; Witkin SS 《Human reproduction (Oxford, England)》1998,13(4):987-990
Two-cell mouse embryos (B6D2F1) were cultured in the presence or absence of
100 microg/ml monoclonal antibodies specific for the mammalian 60 kDa
(HSP60), 70 kDa (HSP70) and 90 kDa (HSP90) heat shock proteins. Embryo
development was evaluated after 3, 5 and 7 days in culture by determining
the number of blastocysts, hatched blastocysts and outgrown trophoblasts at
the successive time points. At day 3, only 29% (22/75) of the embryos
cultured with anti-HSP60 antibody developed to the blastocyst stage (P <
0.0001) as compared to 67% (31/46) of the embryos cultured with anti-HSP70,
72% (43/60) cultured with anti-HSP90, and 79% (49/62) in medium plus mouse
IgG1. By day 5, hatched embryos were present in 28% (13/ 46) of the
cultures containing anti-HSP70 (P < 0.0001), as opposed to 57% (34/60)
containing anti-HSP90 and 73% (45/62) containing IgG1. At day 7, outgrown
trophoblasts were observed in 9% (4/46) of cultures containing anti-HSP70
(P < 0.0001), 45% (27/60) containing anti-HSP90 (P < 0.01) and 66%
(41/62) cultured in medium plus IgG1. Antibodies to different heat shock
proteins exerted a detrimental effect on mouse embryo development at unique
development stages. Immune sensitization to heat shock proteins may be a
cause of reproductive failure.
相似文献
98.
99.
First trimester development of human chorionic villous vascularization studied with CD34 immunohistochemistry 总被引:3,自引:3,他引:3
te Velde EA; Exalto N; Hesseling P; van der Linden HC 《Human reproduction (Oxford, England)》1997,12(7):1577-1581
Normal chorionic villous vascularization is essential for the undisturbed
development of pregnancy. Defective vasculogenesis may play a role in
pathological pregnancy. To assess pathological chorionic villous
vascularization, normal vascularization has to be defined first. Few data
are available on this topic. The aim of this study was therefore to
investigate normal chorionic villous vascularization in ultrasound-dated
first trimester pregnancies from week 5 menstrual age to week 12 (n = 41),
using quantitative CD34 immunohistochemistry. Two important processes in
chorionic villous vascularization were quantitatively illustrated: (i)
maturation, reflected by an increase of the total number of luminized
vessels as opposed to non-luminized haemangioblastic cords and (ii)
margination, due to a decrease of villous stromal area and an increase of
total villous vascular area. The percentage of villous stromal area
occupied by vascular elements (area difference %) increased from 0.7% in
week 5-2.5% in week 10. Therefore, the area of the villous stroma occupied
by vascular elements increases and the vessels are situated closer to the
trophoblastic layer suitable for fetal-maternal exchange. There was also a
trend in increased number of peripheral vessels (2.0 in week 5 to 4.6 in
week 10), supporting both developmental mechanisms. In conclusion, in
exactly dated normal human first trimester pregnancies, development of the
chorionic villous vascular system seems to be mostly characterized by
maturation of luminized vessels from primitive haemangioblastic cords, and
margination to a situation of peripherally located vessels.
相似文献
100.
血清反复冻融对HBsAg和抗HBs抗体检测的影响 总被引:1,自引:0,他引:1
0 引言 EL ISA技术已在乙肝标志物的检测中已普遍应用 ,一般认为收集的血清应及时检测 .而在基层单位或在流行病学调查研究中 ,由于血标本收集不集中 ,或者有些标本常需检测多个指标而持续时间较长 ,因此 ,血清必须低温贮存一定时间或反复冻融后检测 ,而血清冻融对 EL ISA检测结果是否有影响尚不清楚 .为此 ,我们对 183份血清标本冻融前、冻融 3次、冻融 6次后的 HBs Ag和抗 - HBs进行了检测和分析比较 .1 材料和方法1.1 材料 1999- 0 5收集西京医院门诊乙肝 5项检测血清183份 ,年龄、性别、诊断不限 .1.2 方法 于初次检测 … 相似文献