Systemic Besnoitiosis in a Juvenile Roe Deer (Capreolus capreolus)

Herein, we report the first incidence of systemic besnoitiosis in a male juvenile roe deer Capreolus capreolus. The animal was found dead in an area where bovine besnoitiosis is endemic and showed cachexia and multiple skin erosions in the metacarpal and metatarsal areas. Moreover, round and elevated white structures suggestive of Besnoitia spp. tissue cysts were also present. Twenty‐eight tissue samples from different anatomical locations were collected for microscopic lesion and parasite detection through histopathology and PCR. Immunohistochemistry was performed to confirm Besnoitia‐positive reaction in the tissue cysts. In addition, the identity of Besnoitia spp. in PCR‐positive tissue samples was also investigated using microsatellite (MS) markers, and the comparison of protein disulphide isomerase gene sequences (BbPDI) of B. besnoiti and B. tarandi isolated from cattle and reindeer, respectively. Besnoitia cysts were detected in the skin (several parts), respiratory and upper digestive tracts, eyes, kidney, liver, testicle, cardiac muscle and lymphoid tissue. Remarkably, the presence of tissue cysts in the brain confirmed the capacity of Besnoitia spp. to form tissue cysts in the central nervous system (CNS). Finally, the Besnoitia species detected showed the same MS genotype as B. besnoiti, and BbPDI sequences from roe deer and two B. besnoiti isolates were genetically identical throughout multiple sequence alignment. Thus, for the first time, there is evidence that roe deer might act as an intermediate host of B. besnoiti. Further molecular analyses and parasite isolations are needed to corroborate these findings.     

Perceived Stress and Self‐esteem Mediate the Effects of Work‐related Stress on Depression

The aim of the present study was to investigate the impact of perceived stress and self‐esteem on work‐related stress and depression. Two hundred and eighty‐four Korean nurses participated in the study. The participants completed four questionnaires, including the Korean short version of the occupational stress scale, the perceived stress scale, the Rosenberg self‐esteem scale and the Beck depression inventory. Structural equation modelling was used to determine the relationships among work‐related stress, perceived stress, self‐esteem, and depression. Work‐related stress was positively associated with depression. Perceived stress was inversely related to self‐esteem and positively associated with work‐related stress and depression, respectively. Self‐esteem was negatively associated with work‐related stress and depression. Structural equation modelling revealed that self‐esteem and perceived stress fully mediate the relationship between work‐related stress and depression. Future studies should further investigate the effect of psychological characteristics on work‐related stress and symptoms of depression. Copyright © 2012 John Wiley & Sons, Ltd.