~（99m）Tc－新半乳糖白蛋白体外受体介导结合特性的研究

本文报道￣９９ｍＴｃ－新半乳糖白蛋白（￣９９ｍＴｃ－ＮＧＡ）与大鼠肝细胞膜进行体外结合试验。结果表明：￣９９ｍＴｃ－ＮＧＡ系与肝细胞膜上的高亲和力低容量（Ｋ＿１：８．４０８９×１０￣９Ｌ／ｍｏｌ；Ｒ＿１：１．７３５０×１０￣（－１２）ｍｏｌ／ｍｇ）和低亲和力高容量（Ｋ＿２：２．０８２７×１０￣８Ｌ／ｍｏｌ；Ｒ＿２：６．９５３０×１０￣（－１２）ｍｏｌ／ｍｇ）２个结合位点相结合，其结合表现出高特异性、可饱和性及对细胞膜蛋白浓度的线性依赖性，具备受体介导结合的基本特征。￣（９９ｍ）Ｔｃ－ＮＧＡ是一个很有希望的肝受体显像剂。     

Studie über eine alte Leiche von der Zeit der Streitenden Reiche aus dem Grab Nr. 1 am Guo-Jia-Gang in Stadt Jingmen der Provinz Hubei

An ancient femal corpse excavated from the robbed tomb No. 1 at Guo-Jia-Gang in Jingmen city of Hubei Province was studied. It was confirmed that the corpse was buried more than 2 300 years ago, namely at the middle stage of the Warring States period and is the earliest one of the ancient coffin corpses unearthed in China sofar. It is of the same type as the ancient corpses of Weatern Han Dynasty excavated from tomb No. 1 at Ma-Wang-Dui in Changsha City and from the tomb No. 168 at Phoenix Hill in Jinagling City respectively. The collagenous fibers of connective tissue from all parts in the corpse were well preserved, showing fine ultrastructure with clear periodical cross banding, and a lot of bacterial spores were found between them. In addition, a large number of eggs ofclonorchis sinensis andtrichuris trichiura were found in the content of the bowel. The level and the cause of good preservation were discussed and it was believed that the good preservation may resulte from the combined effect of the internal and external environmental factors around the corpse.     

嗅神经母细胞瘤的临床病理及超微结构观察

本文报道了12例嗅神经母细胞瘤的临床病理及其中2例的超微结构特点。光镜观察结果,12例均可见细胞之间由细胞突起形成的嗜酸性背景,有4例可见Flexner菊形团、7例见Homer-Wright菊形团(其中3例同时存在Flexner菊形团);电镜观察结果,细胞具有轴突样和树突样突起,胞体及突起内含微管、神经细丝及神经分泌颗粒,细胞突起之间见突触样的连接。作者并对该瘤的诊断问题进行了探讨。     

流行性出血热患者骨髓细胞超微结构的研究

8例流行性出血热患者骨髓细胞的超微结构研究结果发现骨髓细胞出现变性、空泡、粗面内质网及高尔基体扩张。并在胞浆中发现颗粒-丝状病毒包涵体及直径为100nm的病毒颗粒。同时胶体金免疫电镜发现骨髓细胞EHF抗原阳性,说明骨髓细胞是其繁殖、复制的场所。     

兔膀胱出口部分梗阻所致逼尿肌超微结构的改变

目的　观察兔膀胱出口部分梗阻后逼尿肌细胞超微结构的改变。　方法　建立雄性兔膀胱出口部分梗阻动物模型 ,利用透射电镜观察其逼尿肌细胞内超微结构 ,应用ImagineTool图像分析软件检测粗面内质网面积和线粒体密度。　结果　梗阻组逼尿肌细胞内单位面积平均 1 1 5 .2 8μm2 ,胞质中粗面内质网面积 (5 .377± 2 .31 8) μm2 ,较对照组的 (0 .476± 0 .31 9) μm2 明显扩大 ;线粒体相对密度为 1 .0 2 7± 0 .0 64 ,较对照组的 0 .830± 0 .0 58明显下降 ,P均 <0 .0 1。　结论　膀胱出口部分梗阻后逼尿肌细胞内质网扩张 ,提示其合成蛋白质功能增强 ,从而引起膀胱壁增厚 ;而线粒体水肿明显 ,密度下降 ,提示逼尿肌细胞能量代谢障碍 ,导致其收缩功能下降     

老年糖尿病患者冠状动脉血流储备变化的初步观察

目的　应用彩色多普勒超声仪检测冠状动脉血流储备 (coronaryflowreserve ,CFR) ,初步观察老年糖尿病患者中CFR的变化。方法　 2 5例老年糖尿病患者及 2 5例健康志愿者作对照组 ,比较两组的空腹血糖 (FBG)、早餐后 2h血糖 (P2hBG)、糖化血红蛋白A1c (HbA1c)、总胆固醇 (TC)、低密度脂蛋白胆固醇 (LDL C)及甘油三酯(TG)、内皮素 1(ET 1)、静息状态时患者冠状动脉的基础血流速度 (bFV)、潘生丁注射后的最大冠脉血流速度(mFV)及CFR。结果　糖尿病组的FBG、P2hBG、TG及ET 1水平显著高于对照组 [分别为 :(9 1± 3 3)mmol/L与 (5 3± 0 7)mmol/L ;(15 8± 5 0 )mmol/L与 (8 5± 2 4 )mmol/L ;(2 96± 0 5 6 )与 (1 6 9± 0 82 )mmol/L及(15 3 80± 13 5 0 )ng/L与 (76 2 3± 10 78)ng/L ,均P <0 0 1],糖尿病组静息时的基础冠脉血流速度 (bFV)及TC、LDL C水平与对照组比较 ,差异均无显著性意义 (均P >0 0 5 ) ,而潘生丁注射后mFV及CFR (CFR =mFV/bFV)较对照组明显下降 [分别为 (5 8 1± 7 9)cm/s与 (73 5± 9 8)cm/s及 2 31± 0 4 9与 3 5 8± 0 4 6 ,P均 <0 0 1]。结论　老年糖尿病患者冠状动脉血流储备明显下降。     

骨髓增生异常综合征(附33例临床分析)

本文分析我院33例MDS病例的入院前诊断、病例类型、临床表现、血象及骨髓象资料,其中4例转变为急性白血病。     

新生儿白细胞介素2、γ干扰素水平的研究

通过检测15例正常新生儿、8例早产儿和33例患病新生儿(13例患非感染性疾病,20例患感染性疾病)的白细胞介素2(IL-2)和γ干扰素(IFNγ)水平。并比较两者间的关系。结果发现新生儿IL-2、IFNγ的产生能力较正常成人降低,早产儿降低更明显;感染性疾病能使新生儿IL-2、IFNγ的产生减少,而非感染性疾病对之无明显影响;各组的IL-2和IFNγ同有明显的相关关系。提示评价新生儿的免疫状态应结合淋巴因子水平的测定,临床上对严重感染性疾病的治疗试用IL-2和IFNγ有潜在的重要作用。     

肝靶向半乳糖基干扰素的合成及其生物学性质研究

本文采用２－亚氨基－２－甲氧乙基－１－β－硫代－Ｄ－半乳吡喃糖甙与基因工程干扰素（ＩＦＮａ１）相偶联，得到半乳糖基基因工程干扰素ａ１偶物（Ｇａｌ－ＬＦＮａ１）。并分别进行小鼠体内分布实验和家兔显像实验，其趋肝性实验结果表明，肝脏对Ｇａｌ－ＩＦＮａ１和ＩＦＮａ１的最大摄取值分别为２９．１％和１０．０％，并采用细胞致病变效应（ＣＰＥ）抑制微量法测定了偶联物的效价，偶联物Ｇａｌ－ＩＦＮａ１与对照品（ＩＦ     

THE EFFECT OF ACUPUNCTURE ON PREVENTION AND TREATMENT OF ISCHEMIC ARRHYTHMIA AND THE RELATION BETWEEN EFFECT OF ACUPUNCTURE AND CHOLINERGIC MUSCARINE-LIKE RECEPTORS

For investigating the effect of acupuncture on ischemic arrhythmia and its mech-anism, adult albino rats with ligated anterior descending branch of coronary artery as experimentalmodel were treated with or without acupuncture, and others with imitative operation but without bothcoronary artery ligation and acupuncture treatment were used as control. It was found in acupuncturegroup that the fibrillation-liability of ischemic myocardium was efficiently decreased, the affinity ofAch-M receptors on membranes of ischemic myocardium was markedly increased, and the tolerance ofischemic myocardium to atropine was elevated in the experiment of atropine inducing fibrillation.These results indicate that acupuncture may play a therapeutic role on ischemic arrhythmia throughactivating the activity of muscarine-like receptors of cholinergic nervous system.