全文获取类型
收费全文 | 5926篇 |
免费 | 366篇 |
国内免费 | 54篇 |
专业分类
耳鼻咽喉 | 32篇 |
儿科学 | 178篇 |
妇产科学 | 128篇 |
基础医学 | 834篇 |
口腔科学 | 248篇 |
临床医学 | 445篇 |
内科学 | 1608篇 |
皮肤病学 | 142篇 |
神经病学 | 612篇 |
特种医学 | 188篇 |
外科学 | 489篇 |
综合类 | 9篇 |
一般理论 | 1篇 |
预防医学 | 311篇 |
眼科学 | 27篇 |
药学 | 380篇 |
中国医学 | 5篇 |
肿瘤学 | 709篇 |
出版年
2024年 | 12篇 |
2023年 | 53篇 |
2022年 | 142篇 |
2021年 | 184篇 |
2020年 | 119篇 |
2019年 | 139篇 |
2018年 | 170篇 |
2017年 | 114篇 |
2016年 | 148篇 |
2015年 | 173篇 |
2014年 | 254篇 |
2013年 | 312篇 |
2012年 | 494篇 |
2011年 | 444篇 |
2010年 | 264篇 |
2009年 | 232篇 |
2008年 | 416篇 |
2007年 | 406篇 |
2006年 | 387篇 |
2005年 | 380篇 |
2004年 | 322篇 |
2003年 | 316篇 |
2002年 | 313篇 |
2001年 | 49篇 |
2000年 | 28篇 |
1999年 | 34篇 |
1998年 | 52篇 |
1997年 | 46篇 |
1996年 | 32篇 |
1995年 | 37篇 |
1994年 | 29篇 |
1993年 | 13篇 |
1992年 | 19篇 |
1991年 | 24篇 |
1990年 | 21篇 |
1989年 | 17篇 |
1988年 | 17篇 |
1987年 | 5篇 |
1986年 | 20篇 |
1985年 | 12篇 |
1984年 | 10篇 |
1983年 | 12篇 |
1982年 | 12篇 |
1981年 | 12篇 |
1980年 | 6篇 |
1979年 | 10篇 |
1978年 | 11篇 |
1977年 | 4篇 |
1976年 | 5篇 |
1973年 | 3篇 |
排序方式: 共有6346条查询结果,搜索用时 0 毫秒
91.
Marzia Dolcino Giovanna Zanoni Caterina Bason Elisa Tinazzi Elisa Boccola Enrico Valletta Giovanna Contreas Claudio Lunardi Antonio Puccetti 《Immunologic research》2013,56(2-3):465-476
Celiac disease (CD) is an autoimmune disorder of the small intestine triggered by environmental factors in genetically predisposed individuals. A strong association between type 1 diabetes (T1DM) and CD has been reported. We have previously shown that rotavirus infection may be involved in the pathogenesis of CD through a mechanism of molecular mimicry. Indeed, we identified a subset of anti-transglutaminase IgA antibodies that recognize the rotavirus viral protein VP7. In this study, we aimed at evaluating whether such antibodies may predict the onset of CD in children affected by T1DM. Moreover, to further analyze the link between rotavirus infection and pathogenesis of CD, we analyzed the effect of anti-rotavirus VP7 antibodies on T84 intestinal epithelial cells using the gene-array technique, complemented by the analysis of molecules secreted in the supernatant of stimulated cells. We found that anti-rotavirus VP7 antibodies are present in the vast majority (81 %) of T1DM-CD tested sera, but are detectable also in a fraction (27 %) of T1DM children without CD. Moreover, we found that anti-rotavirus VP7 antibodies are present before the CD onset, preceding the detection of anti-tTG and anti-endomysium antibodies. The gene-array analysis showed that purified anti-rotavirus VP7 antibodies modulate genes that are involved in apoptosis, inflammation, and alteration of the epithelial barrier integrity in intestinal epithelial cells, all typical features of CD. Taken together, these new data further support the involvement of rotavirus infection in the pathogenesis of CD and suggest a predictive role of anti-rotavirus VP7 antibodies. 相似文献
92.
Romagnolo Alberto Balestrino Roberta Imbalzano Gabriele Ciccone Giovannino Riccardini Franco Artusi Carlo Alberto Bozzali Marco Ferrero Bruno Montalenti Elisa Montanaro Elisa Rizzone Mario Giorgio Vaula Giovanna Zibetti Maurizio Lopiano Leonardo 《Journal of neurology》2021,268(3):762-769
Journal of Neurology - Neurological symptoms of COVID-19 patients have been recently described. However, no comprehensive data have been reported on pre-existing neurological comorbidities and... 相似文献
93.
Bucello Sebastiano Annovazzi Pietro Ragonese Paolo Altieri Marta Barcella Valeria Bergamaschi Roberto Bianchi Alessia Borriello Giovanna Buscarinu Maria Chiara Callari Graziella Capobianco Marco Capone Fioravante Cavalla Paola Cavarretta Rosella Cortese Antonio De Luca Giovanna Di Filippo Massimiliano Dattola Vincenzo Fantozzi Roberta Ferraro Elisabetta Filippi Maria Maddalena Gasperini Claudio Grimaldi Luigi Maria Edoardo Landi Doriana Re Marianna Lo Mallucci Giulia Manganotti Paolo Marfia Girolama Alessandra Mirabella Massimiliano Perini Paola Pisa Marco Realmuto Sabrina Russo Margherita Tomassini Valentina Torri-Clerici Valentina Liliana Adriana Zaffaroni Mauro Zuliani Cristina Zywicki Sofia Filippi Massimo Prosperini Luca 《Journal of neurology》2021,268(8):2922-2932
Journal of Neurology - To identify baseline factors associated with disease activity in patients with relapsing–remitting multiple sclerosis (RRMS) under teriflunomide treatment. This was an... 相似文献
94.
Thomas Klopstock MD Aleksandar Videnovic MD Almut Turid Bischoff MD Cecilia Bonnet MD Laura Cif MD Cynthia Comella MD Marta Correa-Vela MD Maria L. Escolar MD Jamie L. Fraser MD Victoria Gonzalez MD Neal Hermanowicz MD Robert Jech MD Hyder A. Jinnah MD Tomasz Kmiec MD Anthony Lang MD Maria J. Martí MD Saadet Mercimek-Andrews MD Migvis Monduy MD Graeme A.M. Nimmo MBBS Belen Perez-Dueñas MD Helle Cecilie Viekilde Pfeiffer MD Lluis Planellas MD Emmanuel Roze MD Nivedita Thakur MD Laura Tochen MD Nora Vanegas-Arroyave MD Giovanna Zorzi MD Colleen Burns PhD Feriandas Greblikas MD 《Movement disorders》2021,36(6):1342-1352
95.
Ercoli Tommaso Masala Carla Pinna Ilenia Orofino Gianni Solla Paolo Rocchi Lorenzo Defazio Giovanni 《Neurological sciences》2021,42(12):4921-4926
Neurological Sciences - Qualitative smell/taste disorders (such as phantosmia, parosmia, phantogeusia, and parageusia) have not yet been fully characterized in patients who had COVID-19, whereas... 相似文献
96.
Marco Romano Monica Sen Cristiano Scottà Rowa Y. Alhabbab Andres Rico-Armada Robert I. Lechler Michael Burch Giovanna Lombardi 《European journal of immunology》2021,51(8):2086-2092
Regulatory T-cells (Tregs) are a subset of T cells generated in the thymus with intrinsic immunosuppressive properties. Phase I clinical trials have shown safety and feasibility of Treg infusion to promote immune tolerance and new studies are ongoing to evaluate their efficacy. During heart transplantation, thymic tissue is routinely discarded providing an attractive source of Tregs. In this study, we developed a GMP-compatible protocol for expanding sorted thymus-derived CD3+CD4+CD25+CD127– (Tregs) as well as CD3+CD4+CD25+CD127–CD45RA+ (RA+Tregs) cells. We aimed to understand whether thymic RA+Tregs can be isolated and expanded offering an advantage in terms of stability as it has been previously shown for circulating adult CD45RA+ Tregs. We show that both Tregs and RA+Tregs could be expanded in large numbers and the presence of rapamycin is essential to inhibit the growth of IFN-γ producing cells. High levels of FOXP3, CTLA4, and CD25 expression, demethylation of the FOXP3 promoter, and high suppressive ability were found with no differences between Tregs and RA+Tregs. After freezing and thawing, all Treg preparations maintained their suppressive ability, stability, as well as CD25 and FOXP3 expression. The number of thymic Tregs that could be isolated with our protocol, their fold expansion, and functional characteristics allow the clinical application of this cell population to promote tolerance in pediatric heart transplant patients. 相似文献
97.
98.
Hector Caballero-Flores Cleber Keiti Nabeshima Giovanna Sarra Maria Stella Moreira Victor Elias Arana-Chavez Márcia Martins Marques Manoel Eduardo de Lima Machado 《Dental materials》2021,37(7):e414-e425
ObjectiveAn ideal scaffold for endodontic regeneration should allow the predictableness of the new tissue organization and limit the negative impact of residual bacteria. Therefore, composition and functionalization of the scaffold play an important role in tissue bioengineering. The objective of this study was to assess the morphological, physicochemical, biological and antimicrobial properties of a new solid chitosan-based scaffold associated with gelatin, microparticulate dentin and genipin.MethodsScaffolds based on chitosan (Ch); chitosan associated with gelatin and genipin (ChGG); and chitosan associated with gelatin, microparticulate dentin and genipin (ChGDG) were prepared by using the freeze-drying method. The morphology of the scaffolds was analyzed by scanning electron microscopy (SEM). The physicochemical properties were assessed for biodegradation, swelling and total released proteins. The biological aspects of the scaffolds were assessed using human cells from the apical papilla (hCAPs). Cell morphology and adhesion to the scaffolds were evaluated by SEM, cytotoxicity and cell proliferation by MTT reduction-assay. Cell differentiation in scaffolds was assessed by using alizarin red assay. The antimicrobial effect of the scaffolds was evaluated by using the bacterial culture method, and bacterial adhesion to the scaffolds was observed by SEM.ResultsAll the scaffolds presented porous structures. The ChCDG had more protein release, adhesion, proliferation and differentiation of hCAPs, and bacteriostatic effect on Enterococcus faecalis than Ch and ChGG (p < 0.05).SignificanceThe chitosan associated with gelatin, microparticulate dentin and genipin has morphological, physicochemical, biological and antibacterial characteristics suitable for their potential use as scaffold in regenerative endodontics. 相似文献
99.
Roberta Roncarati Chiara Viviani Anselmi Peter Krawitz Giovanna Lattanzi Yskert von Kodolitsch Andreas Perrot Elisa di Pasquale Laura Papa Paola Portararo Marta Columbaro Alberto Forni Giuseppe Faggian Gianluigi Condorelli Peter N Robinson 《European journal of human genetics : EJHG》2013,21(10):1105-1111
Familial dilated cardiomyopathy (DCM) is a heterogeneous disease; although 30 disease genes have been discovered, they explain only no more than half of all cases; in addition, the causes of intra-familial variability in DCM have remained largely unknown. In this study, we exploited the use of whole-exome sequencing (WES) to investigate the causes of clinical variability in an extended family with 14 affected subjects, four of whom showed particular severe manifestations of cardiomyopathy requiring heart transplantation in early adulthood. This analysis, followed by confirmative conventional sequencing, identified the mutation p.K219T in the lamin A/C gene in all 14 affected patients. An additional variant in the gene for titin, p.L4855F, was identified in the severely affected patients. The age for heart transplantation was substantially less for LMNA:p.K219T/TTN:p.L4855F double heterozygotes than that for LMNA:p.K219T single heterozygotes. Myocardial specimens of doubly heterozygote individuals showed increased nuclear length, sarcomeric disorganization, and myonuclear clustering compared with samples from single heterozygotes. In conclusion, our results show that WES can be used for the identification of causal and modifier variants in families with variable manifestations of DCM. In addition, they not only indicate that LMNA and TTN mutational status may be useful in this family for risk stratification in individuals at risk for DCM but also suggest titin as a modifier for DCM. 相似文献