全文获取类型
收费全文 | 8059篇 |
免费 | 432篇 |
国内免费 | 68篇 |
专业分类
耳鼻咽喉 | 53篇 |
儿科学 | 219篇 |
妇产科学 | 164篇 |
基础医学 | 799篇 |
口腔科学 | 309篇 |
临床医学 | 580篇 |
内科学 | 2185篇 |
皮肤病学 | 134篇 |
神经病学 | 587篇 |
特种医学 | 423篇 |
外科学 | 1367篇 |
综合类 | 23篇 |
预防医学 | 270篇 |
眼科学 | 58篇 |
药学 | 392篇 |
中国医学 | 11篇 |
肿瘤学 | 985篇 |
出版年
2024年 | 7篇 |
2023年 | 99篇 |
2022年 | 119篇 |
2021年 | 297篇 |
2020年 | 178篇 |
2019年 | 251篇 |
2018年 | 320篇 |
2017年 | 206篇 |
2016年 | 219篇 |
2015年 | 238篇 |
2014年 | 355篇 |
2013年 | 440篇 |
2012年 | 622篇 |
2011年 | 618篇 |
2010年 | 351篇 |
2009年 | 308篇 |
2008年 | 497篇 |
2007年 | 461篇 |
2006年 | 439篇 |
2005年 | 477篇 |
2004年 | 433篇 |
2003年 | 387篇 |
2002年 | 319篇 |
2001年 | 97篇 |
2000年 | 89篇 |
1999年 | 89篇 |
1998年 | 85篇 |
1997年 | 66篇 |
1996年 | 46篇 |
1995年 | 41篇 |
1994年 | 36篇 |
1993年 | 30篇 |
1992年 | 33篇 |
1991年 | 28篇 |
1990年 | 40篇 |
1989年 | 25篇 |
1988年 | 30篇 |
1987年 | 21篇 |
1986年 | 41篇 |
1985年 | 25篇 |
1984年 | 19篇 |
1983年 | 12篇 |
1982年 | 8篇 |
1981年 | 8篇 |
1980年 | 8篇 |
1978年 | 6篇 |
1975年 | 4篇 |
1973年 | 4篇 |
1971年 | 3篇 |
1965年 | 4篇 |
排序方式: 共有8559条查询结果,搜索用时 15 毫秒
61.
Riccardo Asero Alberto Tedeschi Maurizio Lorini Maria Gerosa Pierluigi Meroni Piersandro Riboldi 《Annals of allergy, asthma & immunology》2003,91(1):79-81
BACKGROUND: The nature of histamine-releasing factors involved in the pathogenesis of chronic idiopathic urticaria (CIU) is still controversial, since functional IgG autoantibodies specific for the high-affinity IgE receptor, Fc(epsilon)RI, can be detected in only 20% of patients showing a strong skin reactivity on the autologous serum skin test. The absence of systemic eosinophilia in CIU patients, along with the increase in mast cells in skin biopsy specimens, suggests a possible role for stem cell factor (SCF), the only cytokine/growth factor known to induce mediator release from human mast cells. OBJECTIVE: To investigate the possible role of SCF as a histamine-releasing factor in patients with CIU. METHODS: The SCF levels were measured in serum samples from 65 patients with CIU who scored strongly positive on the autologous serum skin test; of these patients, 32 had negative results and 33 had positive results on in vitro histamine release assay by a quantitative commercial sandwich immunoassay technique. Serum samples from 40 healthy subjects were used as controls. RESULTS: Serum SCF levels in all 65 CIU patients did not differ from those found in healthy controls. No difference in SCF levels was found between patients with positive and negative results on histamine release assay. CONCLUSIONS: An increase in serum SCF levels does not play a pathogenic role in CIU. 相似文献
62.
Mengoli C Cusinato R Biasolo MA Cesaro S Parolin C Palù G 《Journal of medical virology》2004,74(1):78-84
After bone marrow (BM) or solid-organ (SO) transplantation viremic Cytomegalovirus (CMV) infection is observed frequently. Quantitative assay of CMV in blood helps the management of this clinical condition. In the present report, 83 samples from 39 solid organ recipients, three CMV assays were compared simultaneously for the first time: the Nuclisens CMV pp67 assay (nucleic acid sequence-based amplification, NASBA), an "in-house" quantitative real-time PCR assay (TaqMan) for CMV DNA, and pp65 antigenemia. The relation between CMV DNA and pp65 antigenemia, the quantitative assays, was evaluated on a larger group including 251 blood samples from 118 solid organ recipients. Real-time PCR provided the best results; > or =130 CMV DNA copies/2 x 10(5) peripheral blood leukocytes (PBLs) predicted > or =1 pp65 antigen positive (Ag+) cell/2 x 10(5) PBLs. By taking pp65 antigenemia as the "gold standard," the sensitivity of CMV DNA quantitation and of the pp67 RNA assay were 0.95 and 0.20, respectively, while the corresponding specificity values were 0.50 and 0.93. When real-time PCR was considered as the "gold standard," the sensitivity and specificity of the pp65 antigenemia were 0.65 and 0.91, respectively. Among the three tests examined, the sensitivity of the pp67 RNA assay was the lowest. On the other hand, the pp67 RNA assay was highly specific and effective in pinpointing high viremia patients. The present report, by providing predictive values for all three diagnostic profiles, DNA load, antigenemia, and pp67RNA, is a contribution for validation of real-time PCR as a new standard for quantitative assessment of CMV viremia in clinical settings. 相似文献
63.
Antonino Carbone Riccardo Manconi Rachele Volpe Alessandro Poletti Paolo de Paoli Umberto Tirelli 《Virchows Archiv : an international journal of pathology》1986,408(6):637-647
Summary A combined morphological, immunohistological, and enzyme histochemical analysis was performed on frozen and fixed lymph node tissue in a case of histiocytic necrotizing lymphadenitis (HNL) using conventional histology, a panel of monoclonal and polyclonal antibodies, and a series of common haematological enzyme reactions. Histology showed multiple paracortical necrotizing foci which, in a prominently necrobiotic background devoid of granulocytes, contained large numbers of foamy histiocytes and macrophages intermingled with cells resembling degenerating plasmacytoid T-cells. Most of the histiocytes were alpha1-antichymotrypsin positive and foamy cells were also distinctly Leu-M1 positive. Strong granular acid phosphatase (AP) positivity was present in the cytoplasm of the macrophages and histiocytes. The cells with plasmacytoid features showed weaker and homogeneously diffuse AP staining. Alpha-naphthyl acetate esterase (ANAE) activity was much less striking than AP in the necrotizing foci and most of the ANAE negative cells corresponded to those with plasmacytoid features. No cells with B-cell lineage markers were present within the necrotizing foci; most of the occasional T-cells (Leu-1+, Leu-4+) present in the foci were Leu-2a+ (OKT8+) whereas OKT10+ lymphoid cells were abundant and appeared to correspond with the cells with plasmacytoid features. Our combined data confirm that the special type of necrosis found in HNL develops within foci of plasmacytoid T-cells undergoing regressive changes and apparently exhibiting distinct immunohistological and enzyme histochemical features.This study was supported in part by Grant n. 84.00525.44 from Consiglio Nazionale delle Ricerche Progetto Finalizzato Oncologia, Roma and by the Associazione Italiana per la Ricerca sul Cancro, Milano 相似文献
64.
Paenibacillus thiaminolyticus is a nonvirulent organism found in human and ruminant microbiota. However, P. thiaminolyticus can act as an opportunistic pathogen in humans. We describe a case of abdominal wall hematoma secondarily infected by P. thiaminolyticus. Our findings emphasize the risk for unusual Paenibacillus infections in otherwise healthy persons. 相似文献
65.
Cannizzaro Delia Tropeano Maria Pia Milani Davide Spaggiari Riccardo Zaed Ismail Mancarella Cristina Lasio Giovanni Battista Fornari Maurizio Servadei Franco Cardia Andrea 《Neurosurgical review》2021,44(3):1217-1225
Neurosurgical Review - Chordoma is a rare slow-growing neoplastic bone lesion. However, they show an invasive local growth and high recurrence rate, leading to an overall survival rate of 65% at 5... 相似文献
66.
Matthias Lngin Bruno Reichart Stig Steen Trygve Sjberg Audrius Paskevicius Qiuming Liao Guangqi Qin Maren Mokelke Tanja Mayr Julia Radan Lara Issl Ines Buttgereit Jiawei Ying Ann Kathrin Fresch Alessandro Panelli Stefanie Egerer Andrea Bhr Barbara Kessler Anastasia Milusev Riccardo Sfriso Robert Rieben David Ayares Peter J. Murray Reinhard Ellgass Christoph Walz Nikolai Klymiuk Eckhard Wolf Jan‐Michael Abicht Paolo Brenner 《Xenotransplantation》2021,28(1):e12636
67.
Riccardo Schiavina Matteo Droghetti Giacomo Novara Lorenzo Bianchi Caterina Gaudiano Valeria Panebianco Marco Borghesi Pietro Piazza Federico Mineo Bianchi Marco Guerra Beniamino Corcioni Michelangelo Fiorentino Francesca Giunchi Paolo Verze Cristian Pultrone Rita Golfieri Angelo Porreca Vincenzo Mirone Eugenio Brunocilla 《Urologic oncology》2021,39(7):433.e1-433.e7
BackgroundWe aim to evaluate the impact of multiparametric magnetic resonance imaging and fusion-target biopsy for early reclassification of patients with low-risk Prostate Cancer in a randomized trial.Materials and methodsBetween 2015 and 2018, patients diagnosed with Prostate Cancer after random biopsy fulfilling PRIAS criteria were enrolled and centrally randomized (1:1 ratio) to study group or control group. Patients randomized to study group underwent multiparametric magnetic resonance imaging at 3 months from enrollment: patients with positive findings (PIRADS-v2>2) underwent fusion-target biopsy; patients with negative multiparametric magnetic resonance imaging or confirmed ISUP - Grade Group 1 at fusion-target biopsy were managed according to PRIAS schedule and 12-core random biopsy was performed at 12 months. Patients in control group underwent PRIAS protocol, including a confirmatory 12-core random biopsy at 12 months. Primary endpoint was a reduction of reclassification rate at 12-month random biopsy in study group at least 20% less than controls. Reclassification was defined as biopsy ISUP Grade Group 1 in >2 biopsy cores or disease upgrading.ResultsA total of 124 patients were randomized to study group (n = 62) or control group (n = 62). Around 21 of 62 patients (34%) in study group had a positive multiparametric magnetic resonance imaging, and underwent fusion-target biopsy, with 11 (17.7%) reclassifications. Considering the intention-to-treat population, reclassification rate at 12-month random biopsy was 6.5% for study group and 29% for control group, respectively (P < 0.001).ConclusionsThe early employment of multiparametric magnetic resonance imaging for active surveillance patients enrolled after random biopsy consents to significantly reduce reclassifications at 12-month random biopsy. 相似文献
68.
69.
Ester Illiano Francesco Trama Vito Mancini Antonio Ruffo Giuseppe Romeo Filippo Riccardo Consuelo Fabi Giuseppe Carrieri Felice Crocetto Fabrizio Iacono Elisabetta Costantini 《Translational andrology and urology》2021,10(2):555
BackgroundPeyonie’s disease (PD) mostly affects males in the fifth decade of life, with a prevalence in the general population ranging between 0.5% and 20.3%. The pathology of PD is characterized by fibrosis of the tunic albuginea of the cavernous bodies of the penis, with the presence of pain in the erection and penile deformity. This is associated with decreased sexual function for both participants. The objective of the study was to investigate the influence of PD pathology on both male patients’ and their female partners’ sexual spheres, and analyze changes in sexual function and perception following penile correction surgery.MethodsProspective study, we included male patients with PD and their female partner sexually active. Patients underwent corporoplasty with multiple plications. The male and female sexuality was evaluated before surgery and three months after male treatment by the Female sexual Function Index (FSFI); International Index of Erectile Function (IIEF); Visual Analogical Scale (VAS).ResultsFrom January 2018 to November 2019 we included 35 couple. The female subjects before partner’s surgery presented dyspareunia, loss of sexual desire, inability to achieve orgasm, and sexual dissatisfaction. At three months after surgical treatment there was an improvement of sexual function in both male patients and female partners (desire P<0.0001, arousal P<0.0001, lubrification P<0.0001, orgasm P<0.0001, satisfaction P<0.0001, pain P<0.0001). As regarding male patients the pain decreased significantly (VAS score from 6 to 2.5), while there was no statistically significant improvement in erectile function (P=0.05).ConclusionsOur findings suggest that a viable approach to treatment of PD patients that involves their partners could lead to better functional and psychological results. 相似文献
70.
Riccardo Memeo Maria Conticchio Emmanuel Deshayes Silvio Nadalin Astrid Herrero Boris Guiu Fabrizio Panaro 《肝胆外科与营养》2021,10(3):350
Liver resection still represent the treatment of choice for liver malignancies, but in some cases inadequate future remnant liver (FRL) can lead to post hepatectomy liver failure (PHLF) that still represents the most common cause of death after hepatectomy. Several strategies in recent era have been developed in order to generate a compensatory hypertrophy of the FRL, reducing the risk of post hepatectomy liver failure. Portal vein embolization, portal vein ligation, and ALLPS are the most popular techniques historically adopted up to now. The liver venous deprivation and the radio-embolization are the most recent promising techniques. Despite even more precise tools to calculate the relationship among volume and function, such as scintigraphy with 99mTc-mebrofenin (HBS), no consensus is still available to define which of the above mentioned augmentation strategy is more adequate in terms of kind of surgery, complexity of the pathology and quality of liver parenchyma. The aim of this article is to analyse these different strategies to achieve sufficient FRL. 相似文献