全文获取类型
收费全文 | 6138篇 |
免费 | 348篇 |
国内免费 | 19篇 |
专业分类
耳鼻咽喉 | 103篇 |
儿科学 | 168篇 |
妇产科学 | 78篇 |
基础医学 | 880篇 |
口腔科学 | 97篇 |
临床医学 | 519篇 |
内科学 | 1347篇 |
皮肤病学 | 92篇 |
神经病学 | 582篇 |
特种医学 | 259篇 |
外国民族医学 | 1篇 |
外科学 | 1052篇 |
综合类 | 29篇 |
一般理论 | 1篇 |
预防医学 | 597篇 |
眼科学 | 46篇 |
药学 | 390篇 |
中国医学 | 10篇 |
肿瘤学 | 254篇 |
出版年
2023年 | 27篇 |
2022年 | 31篇 |
2021年 | 154篇 |
2020年 | 84篇 |
2019年 | 161篇 |
2018年 | 184篇 |
2017年 | 139篇 |
2016年 | 121篇 |
2015年 | 135篇 |
2014年 | 214篇 |
2013年 | 278篇 |
2012年 | 444篇 |
2011年 | 495篇 |
2010年 | 244篇 |
2009年 | 222篇 |
2008年 | 380篇 |
2007年 | 402篇 |
2006年 | 395篇 |
2005年 | 385篇 |
2004年 | 362篇 |
2003年 | 284篇 |
2002年 | 295篇 |
2001年 | 137篇 |
2000年 | 164篇 |
1999年 | 132篇 |
1998年 | 51篇 |
1997年 | 41篇 |
1996年 | 28篇 |
1995年 | 30篇 |
1994年 | 22篇 |
1993年 | 23篇 |
1992年 | 51篇 |
1991年 | 41篇 |
1990年 | 41篇 |
1989年 | 32篇 |
1988年 | 22篇 |
1987年 | 20篇 |
1986年 | 29篇 |
1985年 | 23篇 |
1984年 | 15篇 |
1983年 | 11篇 |
1979年 | 12篇 |
1978年 | 13篇 |
1975年 | 12篇 |
1974年 | 12篇 |
1973年 | 12篇 |
1972年 | 9篇 |
1971年 | 12篇 |
1970年 | 9篇 |
1968年 | 10篇 |
排序方式: 共有6505条查询结果,搜索用时 234 毫秒
81.
Expression profiling of T-cell lymphomas differentiates peripheral and lymphoblastic lymphomas and defines survival related genes. 总被引:1,自引:0,他引:1
Beatriz Martinez-Delgado Barbara Meléndez Marta Cuadros Javier Alvarez Jose Maria Castrillo Ana Ruiz De La Parte Manuela Mollejo Carmen Bellas Ramon Diaz Luis Lombardía Fatima Al-Shahrour Orlando Domínguez Alberto Cascon Mercedes Robledo Carmen Rivas Javier Benitez 《Clinical cancer research》2004,10(15):4971-4982
PURPOSE: T-Cell lymphomas constitute heterogeneous and aggressive tumors in which pathogenic alterations remain largely unknown. Expression profiling has demonstrated to be a useful tool for molecular classification of tumors. EXPERIMENTAL DESIGN: Using DNA microarrays (CNIO-OncoChip) containing 6386 cancer-related genes, we established the expression profiling of T-cell lymphomas and compared them to normal lymphocytes and lymph nodes. RESULTS: We found significant differences between the peripheral and lymphoblastic T-cell lymphomas, which include a deregulation of nuclear factor-kappaB signaling pathway. We also identify differentially expressed genes between peripheral T-cell lymphoma tumors and normal T lymphocytes or reactive lymph nodes, which could represent candidate tumor markers of these lymphomas. Additionally, a close relationship between genes associated to survival and those that differentiate among the stages of disease and responses to therapy was found. CONCLUSIONS: Our results reflect the value of gene expression profiling to gain insight about the molecular alterations involved in the pathogenesis of T-cell lymphomas. 相似文献
82.
Conde Sánchez JM Rico López J Blasco Hernández P Espinosa Olmedo J Domínguez Domínguez M García Pérez M 《Actas urologicas espa?olas》2000,24(2):182-184
Contribution of a case report of cutaneous horn of penis surgically treated with extensive resection of the implantation base. A well differentiated, microinvasive epidermoid carcinoma was histopathologically demonstrated on a hyperkeratosis squamous papilloma. Although underlying lesions to cutaneous horn are usually benign, malignant changes have been reported in up to one third of cases; surgical treatment should therefore include extensive resection of the implantation base. 相似文献
83.
Expression of Adhesion Molecules and Cytokines on Saphenous Veins in Chronic Venous Insufficiency 总被引:9,自引:0,他引:9
The objective of this study was to assess the relationship of signaling molecules to monocyte/ macrophages as a precursor to venous valve and venous wall dysfunction in patients with varicose veins. One of the hallmarks of venous dysfunction is destruction of venous valves with subsequent reflux and elevation of distal venous pressure. We recently observed that monocytes/macrophages migrate into the venous walls and valves of patients with venous insufficiency. There, they may play a role in the pathogenesis of primary venous insufficiency. If so, an important element in their performance would be the interaction between the monocytes and the endothelium as a precursor of damage to venous valves and the venous wall. To explore this interaction, immunohistochemistry was carried out to detect adhesion molecules and cytokines in surgical specimens removed during surgical therapy. Twenty-four surgical specimens consisting of proximal saphenous vein and subterminal valve were obtained using minimally traumatic technique in 6 males and 18 females who ranged in age from 31 to 79 years. Reflux was confirmed preoperatively by duplex technique, and severity was classified by the CEAP classification of the American Venous Forum. Ten patient limbs were class 2, eight were class 3, four were class 4, and two were class 6. The venous specimens were labeled using monoclonal antibody against ICAM-1, E-selectin, IL-1alpha, and TNF-alpha. CD68 was used for detection of monocytes/macrophages. Our results indicate that not only luminal venous endothelium but also endothelium in the vasa vasora of refluxing saphenous veins is activated, as indicated by the up-regulation of ICAM-1. However, IL-1alpha and TNF-alpha were increased in only selected specimens and are mainly detected in the vein wall. The factors that serve as trigger mechanisms to activate cells in the pathogenesis of primary venous dysfunction remain to be explored. 相似文献
84.
Alvarez B Doménech N Alonso F Sánchez C Gómez del Moral M Ezquerra A Domínguez J 《Xenotransplantation》2000,7(4):258-266
Abstract: We describe in this report the production and characterization of monoclonal antibodies (mAb) to the swine homologues of CD11a and CD18 antigens, and their use for phenotypic and functional analysis of porcine leukocytes. Monoclonal antibodies BL1H8 and BL2F1 precipitated two bands of approximately 170 and 95 kDa, whereas mAb BA3H2 brought down three bands of 170, 155 and 95 kDa, from alveolar macrophage lysates. Clearance of macrophage lysates with mAbs BL1H8 and BL2F1 resulted in complete removal of the 170-kDa band. The cell distribution of the molecules recognized by these mAbs was similar to that of human LFA-1. It was found on all leukocytes, although its expression varied among the different leukocyte subpopulations, with monocytes, granulocytes and a subset of CD8+ cells expressing the highest levels. Cross-blocking studies showed that these antibodies recognize different epitopes on porcine LFA-1. Both anti-LFA-1 mAbs strongly inhibited the mitogenic response of PBMC to ConA, whereas the anti-CD18 mAb had no effect. These anti-LFA-1 mAbs also inhibited the mixed lymphocyte reaction (MLR) and the NK cell-mediated lysis of K-562 cells. 相似文献
85.
Cornelis P. J. Vendrik Anne Marie J. Fichtinger-Schepman Wilhelmina C. M. van Dijk-Knijnenburg W. H. de Jong Anke C. E. van der Minnen Gerard de Groot Geert Frits Berends P. A. Steerenberg 《Cancer chemotherapy and pharmacology》1997,39(6):479-485
An IgM immunocytoma cell line sensitive to cis-diamminedichloroplatinum(II) (CDDP) and a subline with acquired resistance were grown in LOU/M rats. In a previous study
with such rats that had been treated with a high dose of CDDP (10 mg/kg) the tumors did not show differences in cellular platinum
content or DNA-adduct levels, either immediately after treatment or 24 h later. Recently, this high dose was found to overcome
resistance. Therefore, the study was repeated with a 10-fold lower dose (1 mg/kg, i.v.). At 1 and 24 h after treatment, tumor
and kidney tissue were assayed for cellular platinum (atomic absorption spectroscopy, AAS) and DNA platination (immunochemical
detection of the four CDDP-DNA adducts). The results were compared with previous data. All tissues showed a linear response
to dose with regard to platinum uptake as well as adduct formation, with no quantitative difference being seen between the
tumors. Also the relative occurrence of the four adducts was very similar. Between 1 and 24 h, in tumors a substantial decrease
occurred in both platinum content and adduct level; the kidneys showed little reduction, if any. At the lower CDDP dose a
somewhat larger loss of platinum and removal of DNA adducts was observed for the resistant tumor, but these differences could
be explained by “dilution”, as this tumor continues to grow after low-dose treatment (about 20% within 24 h). Since the strong
difference observed between the tumors in sensitivity to CDDP cannot be attributed to differences in CDDP uptake, efficiency
of adduct formation, or repair capability, other mechanisms are held responsible.
Received 10 August 1995 / Accepted: 14 August 1996 相似文献
86.
87.
Juiz JM Luján R Domínguez del Toro E Fuentes V Ballesta JJ Criado M 《The European journal of neuroscience》2000,12(12):4345-4356
Voltage-dependent ion channels have specific patterns of distribution along the neuronal plasma membrane of dendrites, cell bodies and axons, which need to be unravelled in order to understand their contribution to neuronal excitability and firing patterns. We have investigated the subcellular compartmentalization of Kv1.4, a transient, fast-inactivating potassium channel, in fusiform cells and related interneurons of the rat dorsal cochlear nucleus. A polyclonal antibody which binds to a region near the N-terminus domain of a Kv1.4 channel was raised in rabbits. Using a high-resolution combination of immunocytochemical methods, Kv1.4 was localized mainly in the apical dendritic trunks and cell bodies of fusiform cells, as well as in dendrites and cell bodies of interneurons of the dorsal cochlear nucleus, likely cartwheel cells. Quantitative immunogold immunocytochemistry revealed a pronounced distal to proximal gradient in the dendrosomatic distribution of Kv1. 4. In plasma membrane localizations, Kv1.4 was preferentially present in dendritic spines, either in the spine neck or in perisynaptic locations, always away from the postsynaptic density. These findings indicate that Kv1.4 is largely distributed in dendritic compartments of fusiform and cartwheel cells of the dorsal cochlear nucleus. Its preferential localization in dendritic spines, where granule cell axons make powerful excitatory synapses, suggests a role for this voltage-dependent ion channel in the regulation of dendritic excitability and excitatory inputs. 相似文献
88.
89.
Fernando Arias de la Vega Miguel Angel Domínguez Domínguez Ana Manterola Burgaleta Ruth Vera García Maria Eugenia Echeverría Zabalza Eugenio Oria Mundin Enrique Martínez López Pilar Romero Rojano Elena Villafranca Iture 《Clinical & translational oncology》2005,7(2):60-65
INTRODUCTION: This study aims to asses the effectiveness and toxicity of boost radiotherapy concomitant and concurrent cisplatin for patients with locally advanced head and neck cancer (LAHNC). MATERIAL AND METHODS: There were 30 patients included in a prospective, phase II single-institution trial and of whom, 29 were at AJCC stage IV and 1 at stage III. Treatment consisted of radiotherapy acceleration fractionation with concomitant boost, 72 Gy, and 2 cycles of concomitant cisplatin (20 mg/m2/day continuous infusion; days 1-5 and 29-33). Amifostine, (i.v. 200 mg/m2) was administered to 26 prior to the first fraction of radiotherapy. Endpoints of the study were quality-of-life (QL), overall survival, and local control of disease. RESULTS: Complete response (CR) was achieved in 23 patients (77%), 2 patients had partial response (PR) (7%), 4 had no response (13%), and 1 was not evaluated for response. The 2-year overall survival and loco-regional control were 60% and 56%, respectively. Main toxicity was grade 3 or 4 mucositis in 93% of the patients. QL scores (questionnaire QLQC30; version 3.0) and the HN cancer module QLQ-HN35) showed a worsening in areas related to the treatment e.g. dry mouth, problems stretching the mouth, and sticky saliva. CONCLUSIONS: this combination modality is active, but toxic, in the treatment for LAHNC. Concomitant boost radiotherapy is probably, not the best radiotherapy schema for combining with chemotherapy in LAHNC. 相似文献
90.