“120”出诊中急性左心衰的救治体会

目的探讨“120”出诊中急性左心衰竭的救治措施。方法回顾分析34例急性左心衰竭的出诊病例,均经过了病情快速判断、心理安抚、吸氧、辅助端坐体位、心电监护、急救药物治疗、正确转运等处理,少部分患者进行了电除颤、心肺复苏治疗。结果34例患者经过急救处理后病情均不同程度的得到了缓解,安全到达医院者31例,急救有效率91．18％。结论急性左心衰竭是常见的危急重症。提高急救意识、熟练抢救方法、医护担架工积极配合、完善的抢救设备是提高抢救有效率的保证。另外,与患者及家属的积极有效沟通也至关重要,可有效减少医患纠纷。     

MRI成像对子宫内膜癌侵犯肌层的评估

目的探讨MRI对子宫内膜癌肌层浸润深度的评估价值。方法回顾性分析67例经分段刮宫并施行手术治疗的子宫内膜癌患者MRI表现,并与术后病理结果进行对照。结果 MRI判断子宫内膜癌局限于内膜、浸润浅肌层、浸润深肌层及浸透浆膜层的准确性分别为92.5%、80.5%、88.0%、80.5%。结论磁共振可多方位、多序列、多角度成像,对软组织有较高分辨率,对准确判断子宫内膜癌肌层有无浸润具有明显优势。     

ANXA1，Ki-67抗原在宫颈上皮内瘤变和宫颈癌中的表达及其相互关系

目的：探讨膜联蛋白A1（Annexin A1,ANXA1）和细胞核相关抗原Ki-67在正常宫颈上皮、宫颈上皮内瘤变和宫颈鳞状细胞癌组织中的表达及其相关性。方法：应用免疫组化法检测56例宫颈上皮内瘤变（ CIN）,39例早期宫颈鳞状细胞癌（ SCC）和26例正常宫颈鳞状上皮组织中ANXA1和Ki-67的表达,分析两者之间表达的相关性。应用免疫印迹法检测正常宫颈组织、宫颈上皮内瘤变及宫颈鳞状细胞癌组织中ANXA1的蛋白表达情况。结果：在正常宫颈上皮中 ANXA1蛋白呈高阳性表达,随着宫颈病变的进展, ANXA1蛋白表达呈明显下降趋势。在正常宫颈上皮、宫颈上皮内瘤变和宫颈癌组织中ANXA1蛋白表达具有显著性差异（ P＜0.05）。在正常宫颈上皮和CIN组织中Ki-67抗原表达与ANXA1蛋白表达呈负相关（ P＜0.01）。结论：ANXA1蛋白在宫颈癌中低表达,并与抗原表达明显负相关,与宫颈癌发生、发展过程中凋亡抑制和增殖能力增强有关,可以作为预测宫颈鳞癌不良预后因素之一。     

胡黄连苷Ⅱ预处理对大鼠离体心脏缺血再灌注的影响

目的探讨胡黄连苷Ⅱ预处理对大鼠离体心脏缺血再灌注的影响。方法健康Wistar大鼠32只,随机分为对照组（control组）、1μmol／L胡黄连苷Ⅱ预处理组（1-P）、10μmol／L胡黄连苷Ⅱ预处理组和大剂量胡黄连苷Ⅱ预处理组（HP）。采用Langendorff离体心脏流灌装置,行缺血30min,再灌注120min,制备心肌缺血再灌注模型。胡黄连苷Ⅱ预处理于缺血前15min分别以含有1μmol／L和10μmol／L的胡黄连苷lIKH液灌注。记录各组心功能指标,测定心肌梗死面积,测定冠脉灌流出液中超氧化物歧化酶（SOD）、丙二醛（MDA）含量。结果LP组和HP组明显加快心率（HR）、提高左心室发展压（LVDP）和左心室内压最大上升及下降速率（±dp／dt）,缩小心肌梗死范围,提高SOD的活性,降低MDA含量,与对照组比较,差异有统计学意义（P〈0．05）,但HP组较LP组无统计学差异（P〉0．05）。结论胡黄连苷Ⅱ预处理能减轻心肌缺血再灌注损伤,其心肌保护作用机制可能与胡黄连苷Ⅱ提高机体抗氧化应激损伤的能力有关。     

银青口溃含片的薄层色谱鉴别及含量测定

目的:建立银青口溃含片薄层色谱鉴别和含量测定方法.方法:采用TLC法对方中青黛、冰片两味药进行定性鉴别;采用HPLC法对方中金银花的有效成分绿原酸进行含量测定.结果:本品定性鉴别薄层色谱特征明显,专属性强;方法学考察表明,绿原酸进样量在0.043 2 ～0.432 0 μg范围内具有良好的线性关系(r =0.999 9),平均回收率为100.2％(RSD=1.28％,n=6).结论:该法简便、准确、重复性好,可用于银青口溃含片的质量控制.     

Carboxymethylcellulose ammonium-derived nitrogen-doped carbon fiber/molybdenum disulfide hybrids for high-performance supercapacitor electrodes

In this paper, a new type of nitrogen-doped carbon fiber/molybdenum disulfide (N-CFs/MoS₂) hybrid electrode materials are prepared via a certain concentration in solvothermal synthesis followed by a high-temperature carbonization process and using the carboxymethylcellulose ammonium (CMC-NH₄) as a structure-directing agent for MoS₂ nanosheet growth during the solvothermal synthesis process. The addition of CMC-NH₄ effectively prevents the agglomeration of MoS₂ nanosheets to increase the specific surface area. Moreover, it not only serves as a carbon source to provide conductive pathways, but also introduces N atoms to improve the conductivity of the CFs and promote the transfer of electrons and ions. This ultimately increases the conductivity of the electrode materials. Thus, the as-prepared N-CFs/MoS₂ hybrids exhibit excellent electrochemical performance. The specific capacitance is up to 572.6 F g⁻¹ under a current density of 0.75 A g⁻¹ and the specific capacitance retained 98% of the initial capacitance after 5000 cycles of charge–discharge tests at a current density of 2.5 A g⁻¹. Moreover, the hybrids show a maximum energy density of 19.5 W h kg⁻¹ at a power density of 94 W kg⁻¹. Therefore, the as-prepared N-CFs/MoS₂ hybrids with remarkable electrochemical properties, low cost and environment protection show potential for practical application in the development of high-performance electrochemical energy storage devices.

Novel CMC-NH₄-derived nitrogen-doped CFs/MoS₂ hybrid electrode materials are prepared using CMC-NH₄ as a structure-directing agent for MoS₂ nanosheets.     

还原型谷胱甘肽片质量标准提高工作研究

目的:对还原型谷胱甘肽片质量标准进行提高:改进鉴别方法,建立有关物质、溶出度和含量测定的方法。方法:采用反相高效液相色谱法,色谱柱用VenusiL MP C₁₈(4.6 mm×250 mm,5μm),流动相为磷酸盐溶液-甲醇(96:4),检测波长210nm。结果:还原型谷胱甘肽在5~1034μg·mL^-1浓度范围内线性关系良好(r=1.0000,n=7),平均回收率为99.0%,RSD=0.6%。结论:标准修订提高后,可更好地用于该制剂的质量控制。     

苦参片质量标准的研究

目的：修订完善苦参片的质量标准。方法：修订现行标准的显微鉴别和薄层鉴别;增订高效液相色谱法测定苦参片中苦参碱和氧化苦参碱的总量。结果：显微鉴别中苦参组织特征均易察见;薄层色谱中斑点清晰,专属性强;苦参碱,氧化苦参碱分别在24.2～4840μg（r=0.9999）、20.8～4160μg（r=0.9999）范围内线性关系良好,平均回收率分别为97.53%（RSD为1.17%）、96.97%（RSD为1.35%）。结论：修订后的标准简便、准确,可用于苦参片的质量控制。     

NAc Shell Arc/Arg3.1 Protein Mediates Reconsolidation of Morphine CPP by Increased GluR1 Cell Surface Expression: Activation of ERK-Coupled CREB is Required

Background:

Relapse into drug abuse evoked by reexposure to the drug-associated context has been a primary problem in the treatment of drug addiction. Disrupting the reconsolidation of drug-related context memory would therefore limit the relapse susceptibility.

Methods:

Morphine conditioned place preference (CPP) was used to assess activity-regulated cytoskeleton-associated protein (Arc/Arg3.1) and correlative molecule expression in the Nucleus accumbens (NAc) shell during the reconsolidation of morphine CPP. U0126 and Arc/Arg3.1 antisense oligodeoxynucleotide were adapted to evaluate the role and the underlying mechanism of Arc/Arg3.1 during the reconsolidation.

Results:

The retrieval of morphine CPP in rats specifically increased the Arc/Arg3.1 protein level in the NAc shell, accompanied simultaneously by increases in the phosphorylation of extracellular signal-regulated kinase1/2 (pERK1/2), the phosphorylation of Cyclic Adenosine monophosphate (cAMP) response element-binding (pCREB), and the up-regulation of the membrane α-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors GluR1 subunit level. Intra-NAc shell infusion U0126, an inhibitor of the Mitogen-activated protein kinase kinase (MEK), prevented the retrieval-induced up-regulation of pERK1/2, pCREB, Arc/Arg3.1, and membrane GluR1 immediately after retrieval of morphine CPP. The effect of disrupting the reconsolidation of morphine CPP by U0126 could last for at least 14 days, and could not be evoked by a priming injection of morphine. Furthermore, the specific knockdown of Arc/Arg3.1 in the NAc shell decreased the membrane GluR1 level, and impaired both the reconsolidation and the reinstatement of morphine CPP.

Conclusions:

Arc/Arg3.1 in the NAc shell mediates the reconsolidation of morphine-associated context memory via up-regulating the level of membrane of GluR1, for which the local activation of the ERK-CREB signal pathway, as an upstream mechanism of Arc/Arg3.1, is required.