洋葱伯克霍尔德菌群(Bcc)及干扰菌在不同增菌培养基中的生长研究

目的：考察Bcc以及Bcc与干扰菌的混合物在不同的增菌培养基中能否生长和增殖;探讨不同的 Bcc菌种对增菌培养基的营养需求和抗生素的耐受是否存在差异。方法：将Bcc的代表菌株、干扰菌以及 Bcc与干扰菌的混合物分别接种至TSB、10倍稀释的TSB、添加10 mg·L-1庆大霉素的TSB和添加250000 U·L-1多粘菌素B的TSB中培养,划线分离至选择性培养基上观察结果,并对可疑菌落进行分离和鉴定。结果：只接种Bcc的4种增菌培养基均能使之良好地增殖。低量的Bcc(约102 cfu)与高量的干扰菌 (104 cfu)的混合接种,除了神秘伯克霍尔德菌CMCC(B)23010外,4种增菌培养基能使Bcc增殖并被检出。结论：添加抗生素的TSB与未添加抗生素的相比没有表现出明显的优势。TSB可以作为Bcc检验时的增菌培养基使用。生长缓慢且活力弱的Bcc菌株在特定条件下有可能会被漏检。     

岩藻多糖体外抗KRV活性初步研究

目的 为了研究岩藻多糖体外抗Kilham rat virus（KRV）病毒的活性以及抑制炎症因子IL-1、IL-6的表达。方法 CCK-8法测得岩藻多糖对大鼠肾细胞（NRK）的半数细胞毒性浓度（CC50）;通过细胞病变效应测定药物的抗病毒活性,计算药物的半数抑制浓度（IC50）以及治疗指数（SI）;建立KRV感染大鼠肾细胞（NRK）细胞模型,设置阴性对照、病毒感染组以及治疗实验组,通过实时荧光定量PCR检测病毒以及IL-1、IL-6的表达水平。结果 CCK-8法测得药物的CC50 > 2500μg/mL,岩藻多糖的IC50为114.8±8.1μg/mL,治疗指数 > 21.78;qPCR结果表明KRV的转录水平实验组较阳性对照组明显降低,且IL-1、IL-6的转录水平亦显著降低。结论 岩藻多糖具有较小的细胞毒性以及良好的抗KRV病毒的效果,并且能够减轻病毒诱导的炎症反应,为防治病毒感染导致的糖尿病提供理论基础。     

菟丝子定向诱导成骨细胞向神经元细胞转化的研究

目的：开展菟丝子醇提物诱导大鼠成骨细胞向神经元细胞转化的定向诱导研究．方法：采用菟丝子醇提物刺激成骨细胞的分化，用RT-PCR检测和倒置显微镜下观察菟丝子水提物对成骨细胞刺激后的变化。结果：RT-PCR检测有神经元细胞和神经胶质细胞的特异性蛋白神经元烯醇酶（NSE）和胶质纤维酸性蛋白（GFAP）表达，在倒置显微镜下观察到神经元样细胞生长．结论：在体外，菟丝子醇提物具有诱导大鼠成骨细胞向神经元细胞定向分化的作用。     

VVS患者心率变异性、CK、CK-MB变化及治疗前后变化的意义

目的：探讨血管迷走性晕厥(VVS)患者的心率变异性指标、血清肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)的变化及其临床意义。方法：选取我院心血管内科收治的VVS患者67例作为VVS组、领选取70例同龄健康体检对象作为对照组;对比两组的心率、超声心动图参数、心率变异性指标及血清CK、CK-MB水平,并对比67例患者在接受射频导管消融术治疗前后的上述指标变化情况。结果：VVS组患者的最大心率与对照组差异无统计学意义;VVS组患者的最慢心率、平均心率均低于对照组;VVS组的心率总体标准差、差值标准差、差值均方根均高于对照组;VVS组患者的左心房前后径、左心室舒张末内径、左心室射血分数与对照组差异无统计学意义;VVS组患者的血清CK、CK-MB均低于对照组;经射频导管消融术治疗后,VVS组患者的平均心率较治疗前提高;VVS组的心率总体标准差、差值标准差、差值均方根均较治疗前降低;经射频导管消融术治疗后,VVS组血清CK、CK-MB均较治疗前提高。结论：VVS患者的心率变异性更加明显、血清CK、CK-MB水平降低,采用射频导管消融术治疗能显著改善患者的心率变异性。     

SUMOylation of Kir7.1 participates in neuropathic pain through regulating its membrane expression in spinal cord neurons

AimsPotassium (K⁺) channels have been demonstrated to play a prominent involvement in nociceptive processing. Kir7.1, the newest members of the Kir channel family, has not been extensively studied in the CNS, and its function remains largely unknown. The present study investigated the role of spinal Kir7.1 in the development of pathological pain.Methods and ResultsNeuropathic pain was induced by spared nerve injury (SNI). The mechanical sensitivity was assessed by von Frey test. Immunofluorescence staining assay revealed that Kir7.1 was predominantly expressed in spinal neurons but not astrocytes or microglia in normal rats. Western blot results showed that SNI markedly decreased the total and membrane expression of Kir7.1 in the spinal dorsal horn accompanied by mechanical hypersensitivity. Blocking Kir7.1 with the specific antagonist ML418 or knockdown kir7.1 by siRNA led to mechanical allodynia. Co‐IP results showed that the spinal kir7.1 channels were decorated by SUMO‐1 but not SUMO‐2/3, and Kir7.1 SUMOylation was upregulated following SNI. Moreover, inhibited SUMOylation by GA (E1 inhibitor) or 2‐D08 (UBC9 inhibitor) can increase the spinal surface Kir7.1 expression.ConclusionSUMOylation of the Kir7.1 in the spinal cord might contribute to the development of SNI‐induced mechanical allodynia by decreasing the Kir7.1 surface expression in rats.     

Combined prognostic value of preoperative serum thyrotrophin and thyroid hormone concentration in papillary thyroid cancer

BackgroundA growing number of studies have found a close association between thyroid hormones and thyrotrophin (TSH), and they also have prognostic significance in some cancer types; this study aimed to investigate the prognostic value of free triiodothyronine (fT3), free thyroxine (fT4), fT3/fT4, TSH, and their combination in patients with papillary thyroid carcinoma (PTC).MethodsThis study retrospectively analyzed the relevant data of 726 newly diagnosed PTC patients. Both univariate and multivariate analyses were used to predict the recurrence rate, and a risk score was established. In addition, with the use of a random survival forest, a random forest (RF) score was constructed. After calculating the area under the curve (AUC), the diagnostic efficacy of risk score, RF score, and four indicators was compared.ResultsfT3, fT4, fT3/fT4, and TSH were strongly associated with some invasive clinicopathological features and postoperative recurrence. Patients with high expression of fT4 and TSH have a high risk of recurrence. By contrast, patients with high expression of fT3 and fT3/fT4 have a low risk of recurrence. At the same time, the combined use of various indicators is more helpful for establishing an accurate diagnosis. By comparison, we found that the RF score was better than the risk score in terms of predicting the recurrence of PTC.ConclusionThe diagnostic accuracy of a combination of fT3, fT4, fT3/fT4, and TSH can help improve our clinical estimate of the risk of recurrent PTC, thus allowing the development of a more effective treatment plan for patients.     

猫视神经慢性损伤相关差异表达基因消减cDNA文库的构建

目的:构建猫视神经慢性损伤相关差异表达基因消减cDNA文库.方法:15只成年家猫随机分为正常对照组、视神经压迫4周组和视神经压迫8周组(n=5),压迫4周组和压迫8周组猫利用球囊植入法建立慢性视神经损伤模型.取各组动物视神经,TRIzol法提取总RNA,SMART技术合成cDNA,随后利用抑制消减杂交方法分离受压4周和8周视神经中差异表达基因的cDNA片段,将其与T载体进行T/A连接构建文库,将连接产物用氯化钙转化法转化大肠杆菌进行文库扩增和蓝白斑筛选,随机挑取300个白色克隆进行菌落PCR鉴定.结果:菌落PCR扩增显示每个文库80%的克隆中均有200～800 bp的插入片段.结论:成功构建猫视神经慢性损伤相关差异表达基因消减cDNA文库,为进一步筛选、克隆慢性视神经损伤相关差异表达基因奠定了基础.     

Preparation and photodynamic antibacterial/anticancer effects of ultralong-lifetime room-temperature phosphorescent N-doped carbon dots

Room-temperature phosphorescent (RTP) N-doped carbon-dots (CNDs) featuring eco-friendliness, low cost and high biocompatibility, are ideal photodynamic antibacterial and anticancer nanomaterials. However, the existing CNDs are limited by low singlet oxygen (¹O₂) quantum yield, which has become a bottleneck in the development of CNDs. One basic reason is the short T₁-state exciton lifetime of CNDs. Herein, triethylenetetramine hexaacetic acid was used to synthesize CNDs via a one-step hydrothermal method. CNDs are characterized with low toxicity, high biocompatibility and ultralong-lifetime RTP (URTP). In addition to the URTP (average lifetime 414 ms) under solid conditions, CNDs even had URTP (average lifetime 320 ms) in a water environment. The ultralong T₁ exciton lifetime largely extends the collision time between T₁ state excitons and O₂ and prolongs the energy transfer time, not only improving the quantum yield (0.63) of singlet oxygen (¹O₂) in solution, but also facilitating the photodynamic antibacterial and anticancer effects.

The URTP CNDs largely extends the collision time between T₁ state exciton and O₂, which improve quantum yield of singlet oxygen (¹O₂) in solutions, facilitating the photodynamic antibacterial and anticancer effects.     

肝脏血管平滑肌脂肪瘤的CT特征

目的:探讨肝脏血管平滑肌脂肪瘤的CT表现及特征。方法:回顾性分析经手术或穿刺活检证实的7例肝脏血管平滑肌脂肪瘤的CT表现。结果:7例肝脏血管平滑肌脂肪瘤,共发现8个病灶。CT平扫均表现为低密度,其中4个病灶内可测得脂肪密度,CT值<-20HU,1个病灶内可见略高密度出血区及低密度囊变区。增强扫描动脉期8个病灶均显著强化,其中7个病灶为团片状不均匀强化,1个病灶为均匀强化。门脉期6个病灶持续或渐进性强化。5例加行延迟扫描,其中4个病灶仍持续强化。动脉期和/或门脉期6个病灶可见中心血管影。结论:肿瘤内含脂肪成分、动脉期显著不均匀强化、门脉期和延迟期持续或渐进性强化及中心血管影是肝脏血管平滑肌脂肪瘤的特征性CT表现。     

乳猪肝制备肝水解肽的工艺研究

目的以乳猪肝为原料制备肝水解肽，分析产品活性。方法应用酶水解／超滤法提纯制备肝水解肽，采用Folin-酚法、四氮甲唑蓝（MTT）法测定含量及活性，并与成年动物肝脏对比。结果乳猪肝组收率明显高于成年动物肝组，产品活性与后者的比较，极显著性差异。结论采用乳猪肝制备肝水解肽，产品的生物活性高，收率好，具有一定的应用价值？