全文获取类型
收费全文 | 1509篇 |
免费 | 178篇 |
国内免费 | 28篇 |
专业分类
耳鼻咽喉 | 6篇 |
儿科学 | 143篇 |
妇产科学 | 24篇 |
基础医学 | 202篇 |
口腔科学 | 90篇 |
临床医学 | 157篇 |
内科学 | 285篇 |
皮肤病学 | 49篇 |
神经病学 | 33篇 |
特种医学 | 174篇 |
外科学 | 224篇 |
综合类 | 38篇 |
预防医学 | 94篇 |
眼科学 | 40篇 |
药学 | 53篇 |
中国医学 | 2篇 |
肿瘤学 | 101篇 |
出版年
2023年 | 14篇 |
2022年 | 13篇 |
2021年 | 16篇 |
2020年 | 14篇 |
2019年 | 7篇 |
2018年 | 44篇 |
2017年 | 46篇 |
2016年 | 44篇 |
2015年 | 60篇 |
2014年 | 76篇 |
2013年 | 106篇 |
2012年 | 49篇 |
2011年 | 32篇 |
2010年 | 77篇 |
2009年 | 92篇 |
2008年 | 28篇 |
2007年 | 48篇 |
2006年 | 49篇 |
2005年 | 26篇 |
2004年 | 28篇 |
2003年 | 13篇 |
2002年 | 19篇 |
2001年 | 15篇 |
2000年 | 9篇 |
1999年 | 24篇 |
1998年 | 101篇 |
1997年 | 86篇 |
1996年 | 98篇 |
1995年 | 63篇 |
1994年 | 74篇 |
1993年 | 39篇 |
1992年 | 14篇 |
1991年 | 21篇 |
1990年 | 13篇 |
1989年 | 31篇 |
1988年 | 29篇 |
1987年 | 28篇 |
1986年 | 26篇 |
1985年 | 24篇 |
1984年 | 12篇 |
1983年 | 9篇 |
1982年 | 20篇 |
1981年 | 15篇 |
1980年 | 16篇 |
1979年 | 5篇 |
1978年 | 6篇 |
1977年 | 9篇 |
1976年 | 13篇 |
1975年 | 9篇 |
1966年 | 1篇 |
排序方式: 共有1715条查询结果,搜索用时 15 毫秒
151.
Pediatric blood transfusion practice in a tertiary-care pediatric hospital was evaluated retrospectively by using the technique of criteria mapping. A total of 630 transfusion episodes involving red cell concentrates, frozen plasma (plasma frozen within 24 hours of collection), platelet concentrates, and albumin were reviewed: 243 (86.2%) were reviewed only by a technical assistant, and 87 (13.8%) required additional physician review. Of these, 138 were red cell concentrate transfusions: 79.7 percent of that group were considered appropriate, 11.6 percent of unknown benefit/risk ratio, 5.8 percent inappropriate, and 2.9 percent impossible to evaluate. Some 246 frozen plasma transfusions were reviewed: 42.3 percent were considered appropriate, 32.5 percent of unknown benefit/risk ratio, 17.5 percent inappropriate, and 7.7 percent impossible to evaluate. A total of 139 platelet concentrate transfusions were reviewed: 64.7 percent were considered appropriate, 16.5 percent of unknown benefit/risk ratio, 10.1 percent inappropriate, and 8.6 percent impossible to evaluate. Some 107 albumin transfusions were reviewed: 90.6 percent were considered appropriate, 1.9 percent inappropriate, and 7.5 percent impossible to evaluate. The criteria maps developed for this study were easy for the technical assistant to use, and areas of appropriate and inappropriate pediatric transfusion practice were clearly identified. 相似文献
152.
Paltiel HJ; O'Gorman AM; Meagher-Villemure K; Rosenblatt B; Silver K; Watters GV 《Radiology》1987,162(1):115
153.
ZALIN AM; JONES S; FITCH NJS; RAMSDEN DB 《QJM : monthly journal of the Association of Physicians》1991,81(2):945-956
Classification of familial amyloidosis by the chemical natureof the fibrillar protein has become possible. Most such amyloidogenicproteins so far recognized are variant transthyretins, but twokindreds with the same apolipoprotein AI modification have beenreported. We describe the clinical features of another suchfamily in whom petechial skin rash appeared to be a marker forthe disease, which was non-neuropathic and of the Ostertag-type.Immunohistochemistry showed the protein to be apolipoproteinAI, but allele-specific DNA amplification indicated that itwas not the Arg26 variant previously identified. 相似文献
154.
C Defer ; J Coste ; F Descamps ; S Voisin ; JM Lemaire ; M Maniez ; AM Courouce 《Transfusion》1995,35(7):596-600
BACKGROUND: To verify the criteria for human T-lymphotropic virus (HTLV) seropositivity in Western blot (WB) proposed by the Retrovirus Study Group of the French Society of Blood Transfusion, 186 blood donations that were repeatedly reactive in HTLV enzyme-linked immunosorbent assay, selected according to their WB pattern, were tested by polymerase chain reaction (PCR) and radioimmunoprecipitation assay (RIPA). STUDY DESIGN AND METHODS: In two commercially available WBs, 12 samples were confirmed as positive (rgp21+p19+p24) and 174 were interpreted as indeterminate (one or two reactivities to these proteins). The primer pairs used for the PCR allowed the amplification of type I (HTLV-I) or type II (HTLV-II) (or both) sequences. The RIPA was performed with two 35S-labeled cell lines: HTLV-I infected HUT 102/B2 and HTLV-II-infected MoT. RESULTS: Of the 12 positive samples, 11 were classified as HTLV-I-positive and one as HTLV-II-positive. Among the 174 indeterminate samples, three (WB pattern: rgp21+, p19+, p24-) were HTLV-I positive in PCR (one of them was positive in RIPA also); the other 171 were HTLV negative. CONCLUSION: In the study of a population in which 97 percent of HTLV infections are due to HTLV-I, these data support the three-protein criteria (rgp21, p19, and p24) for a positive blot reading. No HTLV infection was observed when rgp21 did not react. Consequently, p19 and/or p24 band patterns represent false reactivity and do not require PCR or RIPA confirmation. To discriminate between false- and true-positive results in the absence of MTA-1 or K55 reactivity, PCR and/or RIPA is required only when rgp21 reactivity is associated with one gag band (p19 or p24). 相似文献
155.
JM Beis C Frenay JM André AM Datié A Baumgarten 《Annales de Réadaptation et de Médecine Physique》1996,39(8):527-533
The use of eye-patches allows to modulate the visual information treating process. Twelve subjects with a left unilateral spatial neglect, randomly divided into three groups — non treated, treated by right eye complete patching, treated by right hemifield patching — were assessed at 1 month and 3 months after acute episode, by means of functional and neuropsychological tests. Results in the subjects treated by complete eye-patch [5]show an improvement of all the assessment parameters whatever the unilateral spatial neglect seriousness degree may be. The progression is less convincing in the patients treated by eye-patch in right hemifield. The effects of the different modalities of occultation interpreted on the basis of anatomo-physiological and psychophysiological patterns of attention, suggest the role of ocular occultation in the initial, voluntary and directed, coven attention recovery and secondary of the automatic and divided overt attention. 相似文献
156.
Klebsiella bacteraemia: community versus nosocomial infection 总被引:2,自引:0,他引:2
Yinnon AM; Butnaru A; Raveh D; Jerassy Z; Rudensky B 《QJM : monthly journal of the Association of Physicians》1996,89(12):933-941
In the period 1988-1993, 241 patients had Klebsiella bacteraemia at our
medical centre. The annual number of patients with positive blood cultures
increased from 306 in 1988 to 622 in 1993, representing a 4.5- 6%
positivity rate of drawn cultures. After E. coli, Klebsiella was the
leading cause of Gram-negative bacteraemia. During this period, the
absolute number of Klebsiella bacteraemia increased from 25 in 1988 to 84
in 1993; this represents a true increase in Klebsiellaa bacteraemia, from
6-7% of positive cultures in the late 1980s to 12-13% in more recent years.
There were 210 cases with K. pneumoniae and 31 with K. oxytoca. A
representative sample of 80 records was retrieved and subdivided into two
groups: community-acquired Klebsiella bacteraemia (CAKB) vs.
hospital-acquired Klebsiella bacteraemia (HAKB). Urinary tract infection
was the underlying source of 58% of CAKB vs 28% of HAKB (p < 0.01);
pneumonia occurred significantly more often in HAKB (25%) than in CAKB (7%)
(p < 0.01). In HAKB, as compared to CAKB, serious manifestations of
illness were more common, e.g. shock (65% vs. 37%, p < 0.046) and
respiratory failure (45% vs. 20%, p < 0.046). Overall mortality was 32%,
22% of patients with CAKB died vs. 42% of those with HAKB (p < 0.05).
Multiple drug resistance was very common: only 57% of all Klebsiella
strains were susceptible to gentamicin, 66% to ceftriaxone, 70% to
ciprofloxacin, and 83% to amikacin. The susceptibility rates of Klebsiella
spp isolated from patients with HAKB were significantly lower (p <
0.001). Sepsis due to multiple-drug- resistant Klebsiellaa has become
frequent, carrying significant morbidity and mortality. Restriction of
broad-spectrum antimicrobials in the hospital and the community as well as
implementation of infection control measures are needed to contain this
problem.
相似文献
157.
Infusible platelet membrane microvesicles: a potential transfusion substitute for platelets 总被引:8,自引:0,他引:8
FC Chao ; BK Kim ; AM Houranieh ; FH Liang ; MW Konrad ; SN Swisher ; JL Tullis 《Transfusion》1996,36(6):536-542
BACKGROUND: Several substitutes for intact, viable platelets have been used for transfusion, both to people and in animal models, with varied success. Infusible platelet membrane (IPM) is prepared from human platelets. IPM retains the glycoprotein (GP)lb receptor and has platelet factor 3 activity (procoagulant activity). However, factor V, serotonin, a cytoplasmic marker enzyme (purine nucleotide phosphorylase), GPIIb/IIIa complex, and HLA class I and II antigens are all absent in IPM. STUDY DESIGN AND METHODS: IPM is prepared from outdated platelets. The platelets were disrupted by freezing and thawing; they were washed and heated to inactivate possible viral contaminants, and then the sonicated membrane microvesicle fraction was separated and lyophilized. The hemostatic activity of IPM was measured by its ability to reduce the prolonged bleeding time in thrombocytopenic rabbits. RESULTS: Administration of IPM at a dose of 2 mg per kg results in a substantial reduction in the bleeding time. In a series of 23 experiments, a median preinjection bleeding time of 15 minutes was reduced to 6 minutes within 4 hours after IPM administration. Administration of IPM did show a mild enhancement in the thrombogenicity index, as measured in the Wessler rabbit model. This enhancement is, however, not significant, as a thrombogenicity index value of up to 0.6 is clinically acceptable. CONCLUSION: IPM may have clinical potential as a substitute for platelets in the treatment of bleeding due to thrombocytopenia. 相似文献
158.
Health professionals have to deal with a “wounded society”, for example, violence, natural disasters and displaced people. Shortage of health professional groups and the high use of complementary therapies may reflect professional wounds, such as stress and burnout. Self-care is an important aspect of health professionals’ lives, given modern-day work stressors that can affect an individual's physical, mental and spiritual health. Often people become healers through personal suffering. Each person wounded or not, needs to understand his or her own need to be nurtured, and develop and implement a self-care health programme. Personal and professional reflection are important to understanding the nature of events that lead to “wounds” and how they can be transcended and the experiences used in holistic care. Aromatherapy can be a useful addition to self-care especially in managing stress and minor self-limiting conditions. 相似文献
159.
壳聚糖/聚乙二醇琥珀酸酯薄膜的制备及其与肌成纤维细胞的相容性 总被引:1,自引:0,他引:1
目的:制备防粘连壳聚糖/聚乙二醇琥珀酸酯薄膜并观察其与肌成纤维细胞的相容性。方法:实验于2006-05/11在南方医科大学附属珠江医院中心实验室完成。实验材料:在透析后的壳聚糖与聚乙二醇琥珀酸酯或聚乙二醇共混后置入冻干机冻干制得壳聚糖/聚乙二醇琥珀酸酯薄膜或壳聚糖/聚乙二醇膜,并将新生2~5d的SD大鼠骨骼肌成纤维细胞种植于膜片上。实验评估:①MTT法测定肌成纤维细胞接种在不同膜片上的吸光度值,计算相对贴附率。相对贴附率=不同膜的A490nm/培养板的A490nm×100%。②MTT法测定肌成纤维细胞在不同膜片上的生长1,5d后的吸光度值。③相差显微镜下观察肌成纤维细胞的生长形貌。结果:①肌成纤维细胞在不同膜片上的贴附率:肌成纤维细胞在壳聚糖/聚乙二醇琥珀酸酯薄膜上能良好黏附、增殖,而在壳聚糖/聚乙二醇膜、壳聚糖膜上黏附性差。联合培养12h,5d后MTT法结果显示,壳聚糖/聚乙二醇琥珀酸酯组的A值分别为0.074±0.009,0.141±0.031,分别为壳聚糖组的6.17倍和6.13倍(P〈0.05)。②肌成纤维细胞的生长特性:肌成纤维细胞在壳聚糖/聚乙二醇琥珀酸酯膜上的活性最高,增殖能力最强,增长速度最快,其次为壳聚糖/聚乙二醇膜,但两者差异无显著性意义(P〉0.05),而细胞在壳聚糖膜上的增殖能力较低,膜上细胞数目较少,与其他组比较差异有显著性意义(P〈0.05)。③肌成纤维细胞与不同膜片联合培养1,5d时的生长形貌:壳聚糖膜上细胞未贴壁生长,为透明的圆球形,呈游离状态,未能很好舒展,且有些皱缩,生长活力也不旺盛;细胞与壳聚糖/聚乙二醇膜、壳聚糖/聚乙二醇琥珀酸酯膜片联合培养的生长情况要明显好于壳聚糖膜,细胞相互融合成片,多呈长梭形,细胞间隙狭窄,紧密排列成束,成指纹状结构且聚集生长的趋势也更明显。结论:将接支琥珀酰基的聚乙二醇与壳聚糖共混组成的网状系统改进了膜片的力学性能,提高了膜片的柔韧性,使其成膜性更好;壳聚糖/聚乙二醇琥珀酸酯薄膜具有良好的生物相容性,肌成纤维细胞在壳聚糖/聚乙二醇琥珀酸酯薄膜上的黏附及生长情况明显好于壳聚糖薄膜。 相似文献
160.