Fura-2/AM 法和 MTT 法筛选多药抗药性逆转剂的比较

为了验证新方法Fura-2/AM法筛选多药抗药性逆转剂的效果,以新方法Fura-2/AM法与传统方法MTT法进行筛选多药抗药性逆转剂的比较研究。结果显示Fura-2/AM法与MTT法的筛选结果基本一致(相关系数γ=0.86,P<0.01),且Fura-2/AM法有操作简单、快速、灵敏、重复性好及测定过程不需无菌操作等的特点,适用于大规模的筛选。     

异位妊娠时宫内膜的临床病理特点

1 临床资料 1990-01/2003-12相关资料齐全的异位妊娠520例.年龄20～48岁.有明显闭经史502(96.5%)例,闭经40 d～3 mo;无明显闭经史18(3.5%)例,但有不规则阴道出血史.     

阿糖腺苷脂质体的制备及其稳定性

本文比较了薄膜法、反相蒸发法、冰冻熔融法制备Ara-A脂质体的包裹率,采用正交设计法摸索Ara-A脂质体制备的最佳技术条件,利用自行设计的改良冰冻熔融法将难溶性药物Ara-A研制成脂质体,其包裹率可达约50%,为国外文献报道的采用反相蒸发法所制得的Ara-A脂质体的包裹率(5.2±0.9%)的10倍。本法操作简单、重现性好。本文还考察了Ara-A脂质体的物理和化学稳定性,实验表明:Ara-A脂质体采用100℃30min灭菌的方法,制品的形态、粒度分布、包裹率及含量均无明显改变,经恒温加速试验,表明Ara-A脂质体具有一定的化学稳定性。     

中药玉竹有效成分研究

自江苏海门产百合科植物玉竹[Polygonatum odoratum(Mill.)Druce]根茎的乙醇提取物中分得六个单体化合物,根据化学性质和光谱解析,鉴定其化学结构分别为β-谷甾醇(S-A)),β-谷甾醇-3-O-β-D-吡喃葡萄糖甙(S-B),25(R,S)螺甾-5-烯-3β-醇-3-O-β-D-吡喃葡萄糖基-(1→2)-[β-D-吡喃木糖基-(1→3)]-β-D-吡喃葡萄糖基(1→4)-β-吡喃半乳糖甙(POD-I),25(R)螺甾-5-烯-3β,14a-二醇-3-0-β-D-吡喃葡萄糖基-(1→2)-[β-D-吡喃木糖基-(1→3)]-β-D-吡喃葡萄糖基-(1→4)-β-D-吡喃半乳糖甙(POD-II),25(R,S)螺甾-5-烯-3β,14a-二醇-3-O-β-D-吡喃葡萄糖基-(1→2)-[β-D-吡喃葡萄糖基-(1→3)]-β-D-吡喃葡萄糖基(1→4)-β-D-吡喃半乳糖甙(POD-III)和25(R,S)螺甾-5-烯-3β-醇-3-O-β-D-吡喃葡萄糖基-(1-2)-[β-D-吡喃葡萄糖基-(1→3)]-β-D-吡喃哺葡萄糖基(1→4)-β-D-吡喃半乳糖甙(POD-IV)。POD-II为首次分离得到的25(R)构型的纯品,POD-III和POD-IV为首次从玉竹中得到。经初步药理实验显示,POD-II有诱生集落刺激因子(CSF)的作用,POD-III能协同ConA和Lps对淋巴细胞转化有促进作用。     

Inhibition of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone-induced lung tumorigenesis by dietary olive oil and squalene

Epidemiological studies have suggested that frequent olive oil consumption may be a protective factor against lung cancer formation. Squalene, a characteristic compound in olive oil, is an inhibitor of 3- hydroxy-3-methylglutaryl coenzyme A reductase activity and has been proposed to inhibit the farnesylation of ras oncoproteins. The present study investigated the effect of dietary olive oil and squalene in a mouse lung tumorigenesis model. Female A/J mice were fed AIN-76A diets containing 5% corn oil (control), 19.6% olive oil, or 2% squalene starting at 3 weeks before a single dose of 4-(methylnitrosamino)-1-(3- pyridyl)-1-butanone (NNK) (103 mg/kg, i.p.). Animals were maintained on their respective diets throughout the study. At 16 weeks after NNK administration, 100% of the mice in the control group had lung tumors with a tumor multiplicity of 16 tumors per mouse. The olive oil and squalene diets significantly (P < 0.05) decreased the lung tumor multiplicity by 46 and 58%, respectively. The squalene diet significantly (P < 0.05) decreased lung hyperplasia by 70%. In mice fed a diet containing 2% squalene for 3 weeks, the activation of NNK was increased by 1.4- and 2.0-fold in lung and liver microsomes, respectively, but its relationship to the inhibition of carcinogenesis is not clear. These results demonstrate that dietary olive oil and squalene can effectively inhibit NNK-induced lung tumorigenesis.      

苏皖地区汉族人群不稳定型心绞痛患者与血小板反应素4基因A387P多态性的关系

目的:观察血小板反应素4基因G29926C(A387P)多态性与中国苏皖地区汉族人群不稳定型心绞痛的可能关系。方法:选择2004-11/2006-05在南京医科大学第一附属医院和江苏大学附属武进医院住院不稳定型心绞痛患者110例,病例均符合2002年AHA/ACC关于不稳定型心绞痛诊断指南的诊断标准,同期选择337例非冠心病者为对照。酚-氯仿法提取白细胞DNA,应用聚合酶链反应-限制性片段长度多态性方法分析血小板反应素4A387P多态性,取部分PCR扩增产物进一步测序鉴定,比较两组间多态性频率分布差异,探讨血小板反应素4基因多态性与不稳定型心绞痛发病的可能关系。结果:447例均进入结果分析。GC基因型在不稳定型心绞痛组和对照组的分布无统计学差异(5.5%,7.1%,P=0.54),未检测到CC纯合子。C等位基因频率在不稳定型心绞痛组和对照组分别为2.7%,3.6%(P=0.55)。GC基因型与不稳定型心绞痛无显著性关联(OR=0.75,95%CI:0.30￣1.89,P=0.54)。结论:血小板反应素4基因387A→P不是中国苏皖地区汉族人群常见的多态位点,且与不稳定型心绞痛的发病无显著相关性。     

Malignant hypertension in young women is related to previous hypertension in pregnancy, not oral contraception

Previous studies have suggested that one-third of women of childbearing age who develop malignant phase hypertension (MHT) are likely to be taking oral contraceptives (OC). We surveyed 104 women with a history of MHT. None of the 65 aged > 45 years were taking OC or other sex hormones. Thirty-nine (mean age 34.9 years, SD 8.0) were aged 15-44 years at presentation: 22 Caucasian, 10 Black/Afro-Caribbean and seven Indo-Asian. Of these 39, 22 had a history of hypertension in pregnancy (group 1), and 17 did not (group 2). Three of group 1 also had a history of OC-induced hypertension. None were pregnant, but one was taking an OC at presentation with MHT. Blood pressures at presentation and follow-up, and mean serum urea and creatinine at presentation were similar between groups, as was median survival (96 vs. 47 months, Lee- Desu statistic 0.75, p = 0.38). There was a trend towards poorer renal function at follow-up in group 1 patients, with higher mean serum urea and creatinine levels. The causes of death were renal failure (5), stroke (4) and heart disease (2). The OC was not a common cause of MHT- amongst our sample of women of childbearing age, but a past history of hypertension in pregnancy was important. Such patients also had a longer duration of hypertension and poorer renal function at follow-up.