630名四川农村成人牙本质敏感流行病学调查分析

目的 了解四川省农村成人牙本质敏感的患病状况,并探讨可能的危险因素.方法 采用多阶段、分层、等容量、随机抽样的方法,对四川省农村630名成人进行牙本质敏感问卷调查和口腔临床检查,使用SPSS 18.0软件对调查数据进行统计分析.结果 四川省农村成人牙本质敏感的患病率为27.9%,酸刺激是最常见的敏感诱发因素.第一前磨牙...     

Studies of fluoride retention by oral soft tissues after the application of home-use topical fluorides.

Previous studies have focused on enamel and plaque as the primary sites of fluoride (F) retention in the mouth. The present study was undertaken to evaluate the role of oral soft tissue as a site of F retention by comparing an edentulous subject panel (n = 9) with a fully dentate panel (n = 10). Unstimulated whole saliva samples were collected by having subjects pool saliva for two min. Samples were collected over a 24-hour period after application of a placebo dentifrice (PD; 0.4 ppm F), fluoride dentifrice (FD; 1100 ppm F), fluoride rinse (FR; 226 ppm F), or fluoride gel (FG; 5000 ppm F) delivered in custom trays. There was no statistically significant difference in salivary flow rate between the two panels for any of the treatments. The edentulous panel had higher salivary F levels than the dentate panel, which reached statistical significance (p less than 0.05) for the FD and FG treatments. In a separate study involving the same treatments, F levels at specific soft-tissue sites were measured over a one-hour period by use of absorbent discs placed in different soft-tissue areas of the mouth. The tongue and lower posterior vestibule retained the highest F levels, followed by the upper posterior buccal vestibule and upper anterior labial vestibule, with the lowest F levels retained in the lower anterior vestibule and the floor of the mouth. There was a strong-to-moderate correlation between whole saliva F concentration and F levels at specific soft-tissue sites. This study establishes the importance of oral soft tissue as the major site of F retention in the mouth.     

Subconjunctival Injection of Regulatory T Cells Potentiates Corneal Healing Via Orchestrating Inflammation and Tissue Repair After Acute Alkali Burn

PurposeThis study aimed to investigate the therapeutic effects and underlying mechanisms of locally delivered regulatory T cells (Tregs) on acute corneal wound healing after alkali burn.MethodsAfter corneal alkali burn, the mice were injected subconjunctivally with regulatory T cells (Tregs) isolated from syngeneic mice. The wound healing process was monitored by clinical manifestation, flow cytometry, and enzyme-linked immunosorbent assay (ELISA). As amphiregulin (Areg) was significantly upregulated, its reparative function in injured corneas was suggested. The hypothesis was further verified via loss- and gain-of-function experiments by administrating the antibody of Areg (anti-Areg) and recombinant Areg (rmAreg).ResultsSubconjunctivally injected Tregs rapidly migrated to injured corneas. The mice treated with Tregs showed prominently reduced corneal opacity, alleviated edema, and faster re-epithelialization compared with the control group. Mechanistically, Treg treatment led to suppressed infiltration of inflammatory cells, along with improved proliferation and inhibited apoptosis of corneal epithelial cells. Tregs expressed upregulated functional markers, including Areg. Expectantly, the levels of Areg in corneas were dramatically higher in the Treg injection group, in line with better corneal restoration. Additional experiments showed that the administration of anti-Areg blunted the reparative effect of Tregs, while exogenous Areg enhanced it. Treg-treated corneas also exhibited less neovascularization and fibrosis at a later reconstruction stage of corneal repair.ConclusionsThe findings showed that the subconjunctival injection of Tregs effectively promoted corneal wound healing by inhibiting excessive inflammation and enhancing epithelial regeneration, with an indispensable reparative role of Areg. Subsequent complications of corneal vascularization and fibrosis were therefore reduced.     

PG消毒剂的研究

PG 消毒剂含2%强化中性戊二醛,醇类和香料.该消毒剂对细菌、真菌与 HBsAg 有良好灭活作用.以其制成消毒纸巾擦手1分钟,可将自然菌减少99%.     

Therapeutic experience with primary liposarcoma from the sigmoid mesocolon accompanied with well-differentiated liposarcomas in the pelvis

Multifocal liposarcoma with different histological types is rare, especially that originating from the mesentery. We herein report a case of primary myxoid liposarcoma from the sigmoid mesocolon that was accompanied with well-differentiated liposarcomas in the pelvis. The location, the fat signal and the relationship with adjacent organs of the liposarcomas were well shown in the axial, coronal and sagittal dimensions of MRI, giving a comprehensive and specific image before surgery. To alleviate the patient’s symptoms and mental stress, a laparotomy was performed. The tumors were all completely excised with macroscopic free margins. The final histopathological report showed that the tumor in the sigmoid mesocolon was a pure myxoid liposarcoma, while the pelvic tumors were spindle cell liposarcomas, a special type of well-differentiated liposarcoma. According to the AJCC staging system, they were all stage IIB. No further adjuvant therapy was performed. Close follow-up after the surgery has been performed, and the patient has remained healthy without any evidence of recurrence or metastasis for 17 months after the surgery.     

汗腺发生及形态结构在汗腺重建中的可能性

目的:通过对汗腺发生、形态、结构以及神经支配和汗液产生及汗腺细胞电生理实验的归纳总结,加深对汗腺再生与重建理论依据的认识。资料来源:应用计算机检索Medline1990-01/2005-01与汗腺发生、形态、结构以及神经支配及汗液产生机制的相关文章,检索词“sweatgland,structure,function”,并限定文章语种为English。同时计算机检索清华全文期刊数据库1990-01/2005-01与汗腺发生、形态、结构、神经支配及汗腺细胞电生理实验的相关文章,检索词“汗腺、发生、结构、功能”,限定语种为中文。资料选择:对资料进行初审,并查看每篇文献后的引文。纳入标准:文章所述内容应与汗腺发生、形态、结构、神经支配及汗液产生相关。排除标准:重复研究或Meta分析类文章。资料提炼:共收集到510篇相关文献,140篇文献符合纳入标准,排除的370篇文献是由于内容陈旧或重复。资料综合:140篇文献中,86篇涉及汗腺发生、形态与结构,31篇涉及汗腺的神经支配,23篇涉及汗液产生及汗腺细胞电生理实验。①汗腺的发生是一个非常复杂的过程,有赖于表皮基底膜区的生理性稳定与真皮-表皮间的相互作用,是基质金属蛋白酶-2和基质金属蛋白酶-7与细胞外基质、表皮生长因子共同协调作用作用的结果。②汗液从汗腺的分泌部分泌时为近等渗液,流经导管时,钠、氯和一些水分被重吸收,使汗液的成分发生变化,最终以低渗液从皮肤表面排出。③汗腺导管细胞和分泌细胞具有不同的电生理现象,正常状态下分泌细胞静息膜电位明显高于导管细胞。④大量体外实验成功分离并培养了汗腺的导管细胞、分泌细胞以及肌上皮细胞,并对其形态发生、生长调控及分化与功能进行探讨以期重建汗腺。结论:由于表皮干细胞与汗腺在发育学上有共同的起源,有可能利用表皮干细胞向汗腺细胞定向分化,从而再生汗腺。另外大面积烧伤患者仍有部分残留的汗腺,有可能通过加速汗腺再生速度和消除瘢痕组织中的屏障因素来重建汗腺。     

早期康复对脑卒中患者功能活动能力的影响

采用FIM量表对68例急性脑卒中患者功能活动能力进行临床对照研究。两组患者在病后3月时的功能活动能力均有不同程度的改善，但康复组优于对照组（P＜0．001），在恢复独立功能活动者中，康复组16例（47．06％），对照组4例（11．76％）。研究结果表明，早期康复干预有助于脑卒中患者独立功能活动能力的恢复；患者在病后1月内，FIM评定为72．25±20．96分考，经早期康复治疗有可能达到功能活动独立。