细胞培养中支原体污染的去除

在运用细胞培养手段的生物学研究和生物工程产品中，支原体污染仍是一个颇为棘手的问题。１１株人的细胞ＳＰＣ－Ａ１、Ｋ５６２、ＨＵＴ－１０２、ＢＴ－３２５、６Ｔ－ＣＥＭ、ＮＫＭ－４５、ＣＮＥ、ＨＬ－６０、ＱＧＹ－７７０１、ＱＺＧ、人羊膜细胞；３株小鼠细胞Ｐ３８８－１、ＹＡＣ－１、Ｐ８１５；１株绒猴细胞Ｂ９５－８；和一株杂交瘤ＱＫＴ３等，已污染了支原体的细胞经ＭＣ－２１０ １μｇ／ｍｌ处理７天，支原体污染     

ASODN对白细胞抗原Ⅰ类基因表达的影响

目的为观察针对乙型肝炎病毒（ＨＢＶ）ｘ基因特异性反义核酸（ＡＳＯＤＮ）对２２１５细胞表面白细胞抗原Ⅰ类基因（ＨＬＡ－Ⅰ）表达的影响。方法人工合成互补于ｘ基因关键区的三段反义核酸，用流式细胞仪检测ＡＳＯＤＮ对２２１５细胞表面ＨＬＡ－Ⅰ表达的影响，细胞原位杂交观察作用细胞内ＨＬＡ－ⅠｍＲＮＡ含量变化，同时用ＰＡＰ－ＥＬＩＳＡ法检测作用细胞上清中ＨＢｘＡｇ的含量。结果三段ＡＳＯＤＮ均能抑制ＨＢｘＡｇ的表达，２２１５细胞表面ＨＬＡ－Ⅰ类抗原的表达及细胞内ＨＬＡ－ⅠｍＲＮＡ含量也降低。结论ＨＢｘＡｇ表达量的降低可能系ＡＳＯＤＮ序列特异性抑制作用所致，而ＨＬＡ－Ⅰ表达的降低可能是ＨＢｘＡｇ对ＨＬＡ－Ⅰ基因启动子的激发减少的间接结果。     

时窗复杂度序列在睡眠脑电分期中的应用

运用时窗复杂度序列来分析睡眠脑电，减少了非平稳性及状态空间的不均匀性造成的脑状态信息的丢失，在一定程度上克服了复杂度自身的局限，有助于不同睡眠期状态特征的提取。另外采用独立分量分析（ICA），小波变换等方法对脑电进行预处理，实验表明它们能有效地去除脑电中的一些生理干扰，有利于提高复杂度算法在睡眠分期应用中的精确度。     

亚甲基四氢叶酸还原酶基因多态性与冠心病关系的研究

目的探讨Ｎ５－亚甲基四氢叶酸还原酶（ＭＴＨＦＲ）基因多态性与冠状动脉粥样硬化性心脏病的关系。方法利用聚合酶链反应和限制性片段长度多态性（ＰＣＲ－ＲＦＬＰ）方法，检测了７９名健康人和６９名冠心病患者ＭＴＨＦＲ基因的６７７碱基多态性突变Ｃ→Ｔ情况，并加以对照分析。结果冠心病患者ＭＴＨＦＲ基因突变型Ｖ６７７基因的频率，明显高于健康人，差异极显著（Ｐ＜０．０１）。结论提示ＭＴＨＦＲ基因突变型Ｖ６７７基因可能是冠心病的又一个遗传风险因子，为探讨冠心病发病机理提供了新的依据     

Monoclonal ribosomal P autoantibody inhibits the expression and release of IL-12, TNF-alpha and iNOS in activated RAW macrophage cell line

Monoclonal ribosomal P protein antibody (anti-P mAb) may bind to the cell surface, penetrate into cells, and induce apoptosis of Jurkat T cells. Recently, modulation of cytokines has been considered to be important in the pathogenesis of systemic lupus erythematous (SLE). In this study, effects of anti-P mAbs (9B6) on gene expression of cytokines, apoptosis, and reactive oxygen species in murine macrophage RAW 264.7 were analyzed by RT-PCR and ELISA and those on IL-12 promoter activity was determined in an IL-12p40 promoter-reporter gene transfected cell line RAW (IL-12p40-SEAP). After treating LPS-activated RAW 264.7 with 9B6 for 6 or 24 h, the levels of mRNA and protein expression of IL-12, TNF-alpha, and iNOS were significantly inhibited by 25%, 16%, and 13%, respectively. The IL-12 promoter activity of RAW (IL-12p40-SEAP) was also inhibited by 13-22%. However, inhibitory effects were not observed in cells pre-treated with IgG1 for 1 h. The productions of IL-10 in LPS-activated RAW 264.7 and human macrophages were potentiated by 9B6 up to 65% and 51%, respectively. Since anti-P Abs inhibit productions of IL-12 and TNF-alpha and enhance IL-10 production in macrophages, these autoantibodies may augment Th2 responses and amplify lupus manifestations by causing immunological polarity and lymphocyte dysfunction.     

兔肺灌洗型呼吸窘迫综合症模型和猪肺表面活性物质的治疗作用

用整肺灌洗法复制兔ＲＤＳ模型，系统观察了灌洗液磷脂和蛋白质含量、血气、潮气量、肺顺应性、生命体征、Ｘ线胸片和病理形态学指标。结果显示：整肺灌洗法可迅速导致严重呼吸窘迫，其病理特征主要是ＰＳ系统功能急剧缺乏和肺损伤，用猪肺表面活性物质治疗后可获得明显改善。表明该模型病理性质介于ＡＲＤＳ和ＮＲＤＳ之间，用我们研制的猪肺表面活性物质替代治疗有较满意的疗效。     

儿童臀肌挛缩症免疫发病机理的研究

本文对４１例儿童臀肌挛缩症患者外周血及３２例儿童臀肌挛缩症臀肌进行了系统的免疫学研究。结果表明：儿童臀肌挛缩症患者免疫调节功能紊乱，红细胞免疫功能低下，臀肌组织免疫复合物沉积，臀肌小血管数目减少及管壁损伤。由此认为免疫病理因素是儿童臀肌挛缩症的主要发病机理。     

Changes of blood humoral substances in experimental cirrhosis and their effects on portal hemodynamics

The changes of humoral substances in the blood of cirrhotic rats were studied together with their effects on portal hemodynamics at different stages during the development of cirrhosis. The profiles of humoral substances and hemodynamics in two different cirrhotic rat models were also investigated. During the development of cirrhosis, glucagon increased markedly in all stages, histamine and vasoactive intestinal polypeptide (VIP) increased in the early stage, serotonin (5-HT) and somatostatin (SS) increased in the middle and late stages. There were different patterns of humoral substances in different cirrhotic models. Glucagon was the main humoral substance elevated in CCL4 induced cirrhosis, but histamine and 5-HT were mainly elevated in the blood in thioacetamide (TAA) induced cirrhosis. The hemodynamics altered differently in different stages during the development of cirrhosis and differently in the two cirrhotic rat models. Exchange transfusions between normal and cirrhotic rats resulted in an elevation of portal flow in normal rats, but no such changes were found after exchange pressure and an increase of portal blood transfusions between normal rats. The relationship between the humoral substances and portal hemodynamics is discussed. The results of this study strongly support the hypothesis of "humoral mechanism" in the pathogenesis of portal hypertension due to cirrhosis.

     

正常人和脑梗死患者颈动脉血流超声多普勒时频分析

目的：分别对正常人和脑梗死患颈动脉血流超声多普勒时频分析,为临床应用提供参考资料。方法：检测正常人和脑梗患（大多有颈动脉粥样硬化斑块）的颈动脉多普勒血流时频谱,以其峰值频率（fmax),模式频率（fmode),平均频率（fmean),低值频率（fmin),重脉波频率（fp)和频弥散宽度（fw)为定量指标。结果：正常人颈动脉血流信号时频谱的fmax,fmean和fmode曲线在一个心动周期中具有总体相似的形状,相互嵌套,其中收缩期的fmax最高,均大于3kHz,fmin最低不低于1kHz,脑梗死患颈动脉时频谱的fmax与fmean和fmode两曲线分开,fmax减低（＜3kHz),fp消失,舒张末期fmin减低显（均低于0．5kHz),结论：颈动脉多普勒血流时频分析及所遴选的定量指标对颈动脉粥样硬化斑块十分敏感,可供诊断试验,且可集成在便携式计算机里,适用于社区人群中筛选易中风病变个体。