氦氖激光对小鼠小脑信息传递物质一氧化氮及环磷酸鸟苷含量的影响

目的：研究低功率氦氖激光对小鼠小脑信息传递物质的影响。方法：采用雄性BALB／C小鼠,随机分为对照组,辐射后即刻组、1h组、2h组,注入氮氖合酶（NOS）抑制剂的辐射后即刻组、副射后1h组,用波长632．8nm、功率密度5mW/cm^2连续氦氖激光每天局部辐射1次,每次1h,连续5d。对照辐射功率密度为0。结果：辐射组分别于辐射后即刻、1h小脑组织中一氧化氮（NO）代谢产物亚硝酸根（NO2^-)及环磷酸鸟苷（cGMP)含量均升高（P＜0．01）,辐射2h二者均降至正常,此增高效应可同时被NOS抑制剂阻断。结论：低功率氦氖激光可使小鼠小脑NO代谢产物NO2^-及cGMP含量短暂性升高,在氦氖激光辐射产生的生物学效应中涉及NO／cGMP信息传递机制。     

乳腺髓样癌的超声检查与组织病理的相关性分析

目的:探讨超声检查在诊断乳腺典型髓样癌中的临床意义。方法:按严格的病理诊断学标准将病例重新分类为乳腺典型髓样癌和非典型髓样癌,比较两者间声像学特征的异同。结果:32例中有13侧重新诊断为乳腺典型髓样癌;另外19例为非典型髓样癌。13例典型髓样癌患者中的10例边界整齐光滑;全部19例非典型髓样癌病人没有一例边界光滑,或者边界呈锯齿状(16例),或者边界光滑伴局部不规则(3例)。非典型与典型髓样癌间肿瘤边界的规则性有明显的差异。结论:如果超声检查提示肿瘤边界呈锯齿状或肿瘤边界光滑伴局部不规则,应行整个肿瘤的病理评估防止典型髓样癌的过诊断。     

异丙酚对人脐静脉内皮细胞凋亡相关基因Bcl-2及Bax表达的影响

目的 :观察异丙酚 (Propofol)及肿瘤坏死因子 α(TNF α)对培养的人脐静脉内皮细胞 (HUVECs)凋亡相关基因Bcl 2及Bax表达的影响 ,探讨异丙酚抑制凋亡的机理。方法 :将培养的人脐静脉内皮细胞 3～ 4代于融合状态 ,随机分为 7组 :对照组 (P0 ) ,2 5 μmol/L异丙酚组 (P2 5) ,TNF α组 (P0 +TNF α) ,12 .5 μmol/L异丙酚和TNF α组 (P12 .5+TNF α) ,2 5 μmol/L异丙酚和TNF α组 (P2 5+TNF α) ,5 0 μmol/L异丙酚和TNF α组 (P50 +TNF α) ,10 0 μmol/L异丙酚和TNF α组 (P10 0 +TNF α)。各组加入相应药物培养 2 4h后收获细胞 ,采用免疫细胞化学染色方法测定Bcl 2及Bax蛋白表达。结果 :与对照组 (P0 )相比 ,异丙酚 2 5 μmol/L组 (P2 5)Bcl 2及Bax蛋白表达无明显变化 (P >0 .0 5 ) ;TNF α(P0 +TNF α)组Bcl 2蛋白表达降低 ,Bax蛋白表达升高 (P <0 .0 0 1) ;而不同浓度的异丙酚预处理后再加入肿瘤坏死因子的各组 (P12 .5+TNF α、P2 5+TNF α、P50 +TNF α、P10 0 +TNF α)Bcl 2蛋白表达增加 ,Bax蛋白表达降低 (P <0 .0 5或P <0 .0 1)。结论 :临床相关浓度的异丙酚可通过调节Bcl 2及Bax蛋白表达抑制TNF α诱导的内皮细胞凋亡     

单态氧猝灭剂叠氮钠治疗角膜碱烧伤实验研究

为研究叠氮钠对角膜碱烧伤的治疗作用,在家兔的角膜碱烧伤模型中,用1 g/L NaN3及10 g/L NaN3+100g/L枸橼酸钠滴眼剂治疗.同时用硫代巴比妥酸(TBA)反应法检测过氧化脂质(LPO),用组织培养人角膜上皮细胞检测NaN3毒性,发现角膜烧伤后LPO逐渐减少.治疗组病情较轻,且NaN3毒性低,认为角膜碱烧伤后,用NaN3治疗有一定效果.     

高效液相色谱法测定辛芩颗粒中黄芩甙的含量

目的：建立辛芩颗粒中黄芩甙的HPLC含量测定方法。方法：用NoVa-Pak C18柱,流动相为甲醇－水－磷酸（45：55：0．2）,在280nm波长处检测。结果：线性范围为0．1793μg-1.076μg。黄芩甙的平均加样回收率为100．94％,RSD为0．46%(n=5)。结论：方法简便、快速、精密度和稳定性, 合于辛芩颗粒生产的质量控制。     

国产硫酸特布他林注射液治疗喘息的临床研究

OBJECTIVE: This multi-centered clinical trial was designed to assess the efficacy and safety of domestic intravenous Turbutalin Sulfate in the treatment of asthma and chronic asthmatic bronchitis. METHODS: Bricanyl was used as control. A total of 120 patients were included in this randomized controlled study. The two medications, both at a dose of 0.25 mg intravenous drip, were given three times a day for 3-5 days. RESULTS: The two drugs' clinical excellent rates were 66.7% and 73.3%; overall efficacy rates were 98.3% and 100.0%, respectively. Their pulmonary functional excellent rates were 78.4% and 76.6%; overall effect rates were 88.3% and 90.0%, respectively. Composite curative evaluation showed that the two drug's composite excellent rates were 61.6% both; their overall efficacy rates were 88.3% and 90.0%, respectively. All of these showed no significant difference (P > 0.05). The incidence rates of adverse side effects of the two groups were 13.5% and 15.0%, respectively; most of them were mild and tolerable. CONCLUSION: Domestic intravenous Terbutalin Sulfate is effective and safe in treating asthma and chronic asthmatic bronchitis.     

尿液结合性胆红素的纯化

OBJECTIVES: To develop a simple, practical method with fine yield and purity for separating conjugated bilirubin(Bc) from a sustainable and facile material in order that it could serve as a calibrator for total and direct bilirubin determination. METHODS: Isolating Bc from urine of patients with obstructive jaundice by CHCl3: CH3CH2OH(1:1, V/V) followed by CNBr activated Sepharose 4B-HAS affinity chromatography, we analyzed the products by the use of Doumas J-G diazo method and HPLC, respectively. RESULTS: Determined by J-G diazo method, the crude product extracted by CHCl3: CH3CH2OH(1:1, V/V) yields 90.2 +/- 5.4%(n = 3) of urine Bc, the ratio of Bc to total dry weight being 67.9 +/- 6.4%(n = 3). The final product purified by affinity chromatography with the ratio of separation of 1.5, 1.737 and analyzed by HPLC has the yield of 46.9% and purity of 94.7%(BDG of 76.2%, BMG of 18.5%). CONCLUSION: The above data demonstrate that urine conjugated bilirubin can be successfully purified by CHCl3: CH3CH2OH extraction followed by Sephrose-HSA affinity chromatography with sufficient purity and output.     

超声多指标预测临产胎儿体重

OBJECTIVE: To evaluate the ultrasonic measurements of parturient fetal cerebellar diameter (CD), liver length(LL), kidney length (KL) and femur thigh soft tissue thickness(FSTT) for the estimation of fetal weight. METHODS: Ultrasonic measurements of the parturient fetal cerebellar diameter, liver length, kidney length and femur thigh soft tissue thickness were made in 115 cases. The data on these parameters were analyzed with statistical methods, and a multiple linear regressional equation for predicting the fetal body weight was derived. The calculated value of fetal body weight and the estimated parameters of the fetal body were compared with the actually measured values immediately after birth. The error of estimation was analyzed. RESULTS: CD, LL, KL and FSTT were significantly correlated with neonatal birth weight, the coefficient of multiple correlation R = 0.9425 (P < 0.001); among the 115 cases, 101 had absolute error less than +/- 200 g, with an agreement rate of 88%. The sensitivity and accuracy for detection of normal fetus were 98.13% and 94.78% respectively. The multiple linear regression equation derived was BW = -1009.914 + 356.1303FSTT + 354.0682KL + 236.8484 LL + 179.7384 CD. CONCLUSION: The equation is a simple, accurate and valuable equation for the estimation of fetal weight and can be used in clinical practice.     

尿液结合性胆红素的纯化

目的　探索一种来源方便 ,具有延续性 ,利于胆红素测定校准物或质控物制备 ,且方法简便、实用、产率和纯度可靠的结合性胆红素 (Bc)纯化方法。方法　应用 CHCl3 ∶ CH3 CH2 OH(1∶ 1,V/V)萃取与 CNBractivated Sepharose4B- HSA亲和层析相结合的方法对胆道梗阻性高胆红素尿液进行分离纯化 ,并以 Doum as J- G法和 HPL C分析产品。结果　由 CHCl3 ∶ CH3 CH2 OH(1∶ 1,V/V)萃取所得的粗纯品经 J- G法检测产率为 90 .2±5 .4% (n=3) ,胆红素含量占总干重的 6 7.9± 6 .4% (n=3)。亲和层析的洗脱峰间的分离度为 1.5、1.737。层析后再萃取所得精纯品经 HPL C分析 ,产率为 46 .9% ,纯度 94.7% ,其中 BDG76 .2 % ,BMG18.5 %。结论　采用有机物萃取和 CNBr activated Sepharose4B- HSA亲和层析相结合的方法纯化结合性胆红素 (BMG& BDG)具有很好的可靠性和可行性     

家兔急性实验性面神经损伤的组织病理观察

目的　观察家兔面神经急性损伤后髓鞘和轴索的组织病理变化。方法　用丝线结扎茎乳孔以外的面神经总干 ,观察结扎后 1天、3天及 5天面神经的组织病理改变。结果　家兔面神经受损后 ,先后在髓鞘和轴索出现轻重不等、程度不同的形态学改变。结论　结扎家兔面神经后 ,其髓鞘及轴索会出现相应的病理改变 ,且随着结扎天数的增加 ,面神经损伤亦呈加重的趋势