Incidence of varying factors on the immunochemical behavior of alpha 1-acid glycoprotein

Electroimmunodiffusion methods of Laurell and radial immunodiffusion method of Mancini are compared for the qualitative and quantitative analysis of native and desialylated alpha 1-acid glycoprotein. Samples are incubated under different conditions at decreasing pH (3.5 to 0.5 pH units), with increasing ionic strength and with neuraminidase during different time intervals. Results show a pronounced decrease in electrophoretic mobility of alpha 1-acid glycoprotein treated either with acidic reagents or with neuraminidase (ionic strength has no effect). Such a procedure might involve chemical or enzymatic hydrolysis by which sialyl residues are removed. This hydrolysis implicates lower results in the estimation of the desialylated glycoprotein by electroimmunodiffusion. On the other hand, the amounts of alpha 1-acid glycoprotein evaluated by radial immunodiffusion are not modified after incubation. This is expected since diffusion and antigenic properties are not related to the sialic acid content. The data suggest that radial immunodiffusion, less accurate and sensitive than electroimmunodiffusion, is nevertheless more adequate for estimating native and desialylated alpha 1-acid glycoprotein.     

Electrophysiological demonstration of an excitatory subthalamonigral pathway in the rat

The subthalamonigral projection was studied with extracellular techniques in the rat. Orthodromic and antidromic stimulations of this pathway were performed in different sets of experiments in order to exclude the possible activation of passing fibers. A majority of the recorded cells in the subthalamic nucleus (STN) were antidromically activated by nigral stimulation. It was furthermore shown that the subthalamofugal fibers are excitatory to cells located in the pars compacta as well as in the pars reticulata of the substantia nigra. The possible existence of another descending STN pathway is also discussed.     

Blood CD34-c-Kit+ cell rate correlates with aggressive forms of systemic mastocytosis and behaves like a mast cell precursor

Mastocytosis is a heterogeneous disease characterized by the accumulation of mast cells in one or more organs. Our objective was to identify a peripheral mast cell precursor and assess its variation rate in mastocytosis. A peripheral blood phenotypic analysis was performed among 50 patients with mastocytosis who were enrolled in a prospective multicentric French study, and the phenotypic analysis results of the patients were compared with those of healthy donors. The rate of peripheral blood CD34(-)c-Kit(+) cells correlated with the severity of mastocytosis. This cellular population was isolated from healthy donors as well as from patients with systemic mastocytosis. After 30 days of culture, the CD34(-)c-Kit(+) cells gave birth to mature mast cells, indicating that this cellular population constitutes a mast cell circulating precursor. Monitoring peripheral CD34(-)c-Kit(+) cells by flow cytometry could be a useful and low-invasive tool to determine the disease severity and the relapses and to assess treatment efficiency.     

急性脑梗死中医治疗现状的思考

简要论述急性脑梗死中医治疗的现状,提出现代中医治疗急性脑梗死应关注的问题,并指出急性脑梗死的发病主要由肝肾不足、脾气亏虚而引发。临床治疗中应重视气虚、肾虚与血瘀的关系,并对活血、凉肝、清热解毒、化痰祛瘀等治法的运用进行探讨。     

中西医结合治疗输卵管阻塞的临床分析

将 32例输卵管阻塞致不孕的患者分成中药组和对照组,两组患者行输卵管疏通术后在应用抗生素治疗的同时,中药组再口服“通管方”和“灌肠 2号方”灌肠,以 1个月为一疗程,进行两组保持通畅率以及妊娠率的比较。结果保持通畅率中药组优于对照组(P<0. 05),妊娠率两组无显著性差异(P>0. 05)。     

Splenic lymphoma with villous lymphocytes involves B cells with extensively mutated Ig heavy chain variable region genes

Splenic lymphoma with villous lymphocytes (SLVL) is a recently defined subgroup of chronic B-cell lymphoproliferative diseases. The characteristic morphology of the tumor cells, together with phenotypic and cytogenetic findings, indicate that it is a distinct entity, but the nature of the cell or origin and its relationship to other low- grade lymphomas is unclear. For B-cell tumors, analysis of the variable region heavy chain (VH) genes used to encode the clonal Ig has shown marked differences between histologic categories, both in gene usage and extent of somatic mutation. An investigation of VH genes used in five typical cases of SLVL has shown somatic hypermutation from germline sequences in all cases, indicating that the cell of origin has been exposed to the hypermutation mechanism. However, no clonal heterogeneity was detectable, demonstrating that the tumor cell does not accumulate further mutations. These characteristics are similar to those found in mature postfollicular B cells, such as plasma cells. The distribution of mutations leading to replacement amino acids differed among the cases, with three of five cases showing clear evidence for antigen selection.