Suspected Biliary Complications After Laparoscopic and Open Cholecystectomy Leading to Endoscopic Cholangiography A Retrospective Comparison

To study how suspected postoperative biliarycomplications are influenced by surgical technique, wecompared clinical profiles of 63 patients referred forERCP after open (OC) and laparoscopic cholecystectomy (LC) over a four-year period. ERCP was notperformed for postoperative pain alone and only six(9.5%) studies were normal. Referrals after LC wereyounger (mean 39.1 vs 53.6 years, P < 0.001) and ERCP was requested earlier (mean 71.6 vs 2360 days,P < 0.001) in the postoperative course.Choledocholithiasis (CDL) alone, the most commonfinding, was successfully managed with a single ERCP in97.2% of cases. CDL after LC occurred in youngerpatients (35.5 vs 58.9 years, P < 0.01) who presentedearlier (mean 98.6 days vs 5.1 years, P < 0.01),without biliary ductal dilatation (P < 0.01).Although CDL after LC was associated with higher ALT andbilirubin levels than after OC, the difference was notstatistically significant. Cystic duct leaks (LC: sixpatients, OC: four patients) were typically associated with CDL after OC and 90% resolved withendoscopic therapy. Biliary ligation (four cases) wasmanaged successfully with choledochojejunostomy. Weconclude that findings at ERCP for suspected biliaryobstruction or injury after OC or LC are similar andusually can be endoscopically managed. After LC,referrals currently are younger, present much earlier,and retained stones are less likely to be associatedwith ductal dilatation than after OC.     

反相高效液相色谱法测定二乙烯三胺五醋酸注射液的含量

目的:研究二乙烯三胺五醋酸三钠锌盐及三钠钙盐注射液的离子对高效液相色谱测定。方法:以ODS(5μm,5mm×150mm)为色谱柱,流动相为甲醇-0.05mol/L醋酸钠缓冲液(pH4.5)(5:95),内含5mmol/L四丁基碘化胺,内标物为乙二胺四乙酸二钠,二乙烯三胺五醋酸(DTPA)及乙二胺四乙酸,(EDTA)与Fe~(3 )螯合成DTPA-Fe(Ⅲ)和EDTA-Fe(Ⅲ)复合物,其检测波长为280nm。结果:二乙烯三胺五醋酸的线性范围在20～320μg/ml,其三钠锌盐和三钠钙盐注射液5次测定的平均回收率±RSD分别为(101.33±0.65)％和(100.59±0.58)％。结论:此法重现性好,灵敏、特异,消除了多种金属离子的干扰。     

Intracellular and plasma magnesium in familial hemiplegic migraine and migraine with and without aura

Familial hemiplegic migraine (FHM) is an autosomal dominant type of migraine and probably represents the most extreme end of migraine with aura. Reduced magnesium facilitates the development of spreading depression and possibly aura. Cellular magnesium levels are under genetic control. We hypothesized that FHM patients would have significantly reduced intracellular magnesium levels. We determined intracellular and plasma magnesium levels in blood of 38 afflicted and 11 non-afflicted members of three families with FHM and, in 32 migraine patients (9 with and 23 without aura) and 32 age and sex matched healthy controls. We found no significant differences between the magnesium levels in the five study groups. We conclude that reduced blood magnesium is unlikely to be related to migraine pathophysiology.     

Fostering disability-inclusive HIV/AIDS programs in northeast India: a participatory study

Background  

Manipur and Nagaland in northeast India are among the Indian states with the highest prevalence of HIV. Most prevention and care programs focus on identified "high risk" groups, but recent data suggest the epidemic is increasing among the general population, primarily through heterosexual sex. People with disability (PWD) in India are more likely than the general population to be illiterate, unemployed and impoverished, but little is known of their HIV risk.     

Observations on the Chromium Labelling of ACD-Stored and Previously Frozen Red Cells

Chromium labelling characteristics of both ACD-stored and previously frozen red cells were evaluated. The chromium uptake of previously frozen red cells processed by agglomeration was inversely related to the hemoglobin level of the suspending fluid. Ascorbic acid was not needed for the labelling of previously frozen, agglomerated red cells.
Cellular injury, as measured by increase in supernatant hemoglobin during post-thaw storage at 4 C, occurred with the agglomerated, previously frozen red cells when: (1) Na₂ EDTA was present in the glycerolizing solution; (2) the disaggregation of the agglomerated red cell mass was carried out with 75 rather than 250 ml of isotonic saline; and (3) the storage temperature of the glycerolized red cells was interrupted for one week with a storage interim at either 4 C or −20 C.
By use of a phthalate ester technic, red cells were separated into three fractions on the basis of cellular density. Preferential chromium labelling of red cells was noted: the lightest fraction contained significantly more radioactivity than the heaviest fraction.