基于轨迹交叉理论的有源医疗器械不良事件致因模型研究

在医疗器械不良事件研究中,运用安全人机工程学的轨迹交叉理论,通过对40例上市后有源医疗器械伤害事件的案例研究,分析和归纳上市后有源医疗器械导致/可能导致伤害事件的发生原因,并依据直接原因,提出了伤害事件分型,从而建立了有源医疗器械不良事件致因模型,以期为上市后有源医疗器械的安全性评价提供基础理论支撑。     

Design,synthesis and activity evaluation of novel pyridinone derivatives as anti-HIV-1 dual (RT/IN) inhibitors

Three series of novel anti-immunodeficiency virus 1 (HIV-1) dual (RT/IN) inhibitors were rationally designed by introducing a functioning diketo acid (DKA) into pyridin-2-one scaffold. To efficiently analyze inhibitory activity, these compounds were screened against HIV-1 RT and IN respectively via surface plasmon resonance (SPR), and active compounds were subsequently evaluated by enzyme assay. It was noteworthy that compound A2 exhibited moderate activity against both HIV-1 RT and IN. This result provided information for further development of pyridinone analogues as potent dual HIV-1 inhibitors.     

龙葵清肝汤配合西药治疗慢性乙型病毒性肝炎临床观察

目的 探讨龙葵清肝汤配合干扰素、拉米夫定治疗慢性乙型病毒性肝炎的疗效.方法 将确诊为慢性乙型病毒性肝炎的住院患者共180例,随机分为治疗组100例和对照组80例.对照组用干扰素、拉米夫定治疗.治疗组在对照组治疗的基础上,加用龙葵清肝汤治疗.评价龙葵清肝汤配合干扰素、拉米夫定治疗慢性乙型病毒性肝炎的疗效.结果 治疗组临床疗效优于对照组,两组比较有显著性差异(P<0.05);乙肝病毒标志物及肝功能复常率治疗组均优于对照组,两组比较有显著性差异(P<0.05).结论 龙葵清肝汤配合干扰素、拉米夫定对慢性乙型病毒性肝炎患者的疗效优于单纯使用干扰素、拉米夫定方法.     

参芪注射液对大鼠失血性休克再灌注肠粘膜糖皮质激素受体的调节作用

目的 探讨参芪注射液对大鼠失血性休克-再灌注后肠粘膜组织糖皮质激素受体的影响.方法 雄性SD大鼠72只,随机分成:正常组、模型组、参芪低剂量组(参芪注射液10g/kg)和参芪高剂量组(参纸注射液20g/kg).对SD大鼠静脉使用肝素后缓慢放血,建立重度血失性休克及复苏的动物模型;参芪注射液于再灌注前静脉注入.造模完成后观察各组动物肠粘膜病理学变化,动态观察2,6、12 h肠粘膜、GR的结合活性和GRRNA的表达水平.结果 正常组肠粘膜无明显改变,模型组肠粘膜重度损伤,参芪高剂量组轻度损伤,低剂量组中度损伤;参芪高剂量组与低剂量组比较,肠粘膜损伤减轻程度更明显.模型组与正常组比较,2、6、12 h肠粘膜GR结合活性和GRmRNA表达明显降低(P<0.01);参芪注射液高低剂量组与模型组比较,2、6、12h肠粘膜GR结合活性和GRmRNA表达明显升高(P<0.01,P<0.05);参芪高剂量组与低剂量组比较,2、6、12h肠粘膜GR结合活性和GRmRNA表达升高更明显(P<0.05).结论 参芪注射液能有效地减轻大鼠失血性休克再灌注肠粘膜损伤,提高肠粘膜GR结合活性和GRmRNA的表达水平.     

2～3岁语言发育迟缓儿童的语言能力与父母述情障碍的关系

目的：探究2～3岁语言发育迟缓儿童的语言能力水平与父母述情障碍的关系。方法：选取2021年1月至6月于温州医科大学附属第二医院就诊的59例2～3岁语言发育迟缓儿童和其父母为研究对象,采用一般资料问卷、语言发育迟缓检查法、Gesell婴幼儿发展量表和多伦多述情障碍量表进行调查。结果：Pearson相关分析发现,父母的述情障碍得分与儿童语言发育迟缓（动作课题、符号理解、语言表达）得分呈显著负相关（P<0.05）。回归分析结果显示,父母的述情障碍越高,儿童语言发育迟缓（符号理解能力、动作性课题能力）能力越差（β=0.62,P<0.01;β=-0.60,P<0.01）。结论：父母的述情障碍越严重,儿童的语言发育迟缓程度越高。     

PGK1-coupled HSP90 stabilizes GSK3β expression to regulate the stemness of breast cancer stem cells

Objective: Glycogen synthase kinase-3β(GSK3β) has been recognized as a suppressor of Wnt/β-catenin signaling, which is critical for the stemness maintenance of breast cancer stem cells. However, the regulatory mechanisms of GSK3β protein expression remain elusive.Methods: Co-immunoprecipitation and mass spectral assays were performed to identify molecules binding to GSK3β, and to characterize the interactions of GSK3β, heat shock protein 90(Hsp90), and co-chaperones. The role of PGK1 in Hsp90 ch...     

Somatic copy number alterations are predictive of progression-free survival in patients with lung adenocarcinoma undergoing radiotherapy

Objective: Lung cancer is the most common cause of cancer-related deaths worldwide. Somatic copy number alterations(SCNAs) have been used to predict responses to therapies in many cancers, including lung cancer. However, little is known about whether they are predictive of radiotherapy outcomes. We aimed to understand the prognostic value and biological functions of SCNAs.Methods: We analyzed the correlation between SCNAs and clinical outcomes in The Cancer Genome Atlas data for 486 patients wit...     

Light Emitting Diodes Irradiation Regulates miRNA-877-3p to Promote Cardiomyocyte Proliferation

Mammalian cardiomyocytes (CMs) maintain a low capacity for self-renewal in adulthood, therefore the induction of CMs cycle re-entry is an important approach to promote myocardial repair after injury. Recently, photobiomodulation (PBM) has been used to manipulate physiological activities of various tissues and organs by non-invasive means. Here, we demonstrate that conditioned PBM using light-emitting diodes with a wavelength of 630 nm (LED-Red) was capable of promoting the proliferation of neonatal CMs. Further studies showed that low-power LED-Red affected the expression of miR-877-3p and promoted the proliferation of CMs. In contrast, silencing of miR-877-3p partially abolished the pro-proliferative actions of LED-Red irradiation on CMs. Mechanistically, GADD45g was identified as a downstream target gene of miR-877-3p. Conditioned LED-Red irradiation also inhibited the expression of GADD45g in neonatal CMs. Moreover, GADD45g siRNA reversed the positive effect of LED-Red on the proliferation of neonatal CMs. Taken together, conditioned LED-Red irradiation increased miR-877-3p expression and promoted the proliferation of neonatal CMs by targeting GADD45g. This finding provides a new insight into the role of LED-Red irradiation in neonatal CMs biology and suggests its potential application in myocardial injury repair.     

荜铃胃痛颗粒联合雷贝拉唑治疗对胃食管反流病临床效果、症候积分及GERDQ评分的影响

目的 观察荜铃胃痛颗粒联合雷贝拉唑治疗对胃食管反流病(GERD)的临床效果、症候积分及胃食管反流病量表(GERDQ)评分的影响。方法 回顾性分析2020年3月—2021年8月收治的GERD 247例的临床资料,根据治疗方案分为A组80例、B组82例、C组85例。A组给予雷贝拉唑治疗8周,B组给予荜铃胃痛颗粒治疗4周联合雷贝拉唑治疗8周,C组给予荜铃胃痛颗粒联合雷贝拉唑治疗8周。比较3组临床疗效、症候积分、GERDQ评分及不良反应发生情况。结果 3组临床总有效率比较差异有统计学意义(P<0.05)。2组治疗后胃灼热、反酸、嗳气或反食症候积分及GERDQ评分均低于治疗前,且C组低于A、B组,B组低于A组(P<0.05)。3组不良反应发生率比较差异无统计学意义(P>0.05)。结论 荜铃胃痛颗粒联合雷贝拉唑治疗GERD可显著提高临床效果,且不增加不良反应。