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Murine peritoneal macrophages, parabiotically co-cultured with combinations of in vitro H-2 sensitized thymus-derived lymphocytes obtained from drug-pretreated mice, possessed an increased cytotoxicity against alloantibody-coated target cells. This heightened activity appeared to be accentuated by and dependent on T-cell synergy. After 5 days of in vitro allosensitization at 37 degrees C, cortisone-resistant thymocytes allosensitized in combination with cyclophosphamide-pretreated splenic T cells released molecules that produced strong antibody-dependent macrophage-mediated cytotoxicity (ADCC). This enhanced ADCC correlated with increased macrophage rosetting with IgG-sensitized erythrocytes. These heightened activities resulted from soluble mediators released by the activated T cells which diffused across a 0.22-microns Millipore filter and were not dependent on lymphocyte-macrophage contact. Evidence that these molecules originated from the highly enriched T-cell populations and were not synthesized de novo by macrophages was supported by results of pretreatment with protein and RNA synthesis inhibitors. Evidence that soluble Fc receptors released from the alloactivated T cells were responsible for the increased macrophage EA binding and ADCC was obtained in affinity chromatography experiments in which activity could be depleted by passage over a Sepharose-Fc-coupled column and recovered in the column eluate. 相似文献
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In 17 patients with Waldenströms Macroglobulinemia, total chromiumspace, red cell mass, plasma volume and relative serum viscosities were determined; aldosterone excretion was determined in 12. Ten of the 15 patients whomanifested elevated serum viscosity at some time during the course of studypresented signs and symptoms of hyperviscosity syndrome. All patients had increased total blood volume. Fifteen had moderate decreases in red cell massbut all had elevations of plasma volume far in excess of that required to compensate for the lowered cell mass. The degree of the increase in plasma volumecorrelated (r = 0.74) with the degree of abnormality in relative serum viscosity. Aldosterone excretion in such patients was normal to low. It is concluded that the plasma volume increase is correlated with serum viscosity andis mediated by sodium retention mechanisms not involving modification ofaldosterone secretion. 相似文献
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HH Chen MR Phillips H Cheng QQ Chen XD Chen D Fralick YE Zhang M Liu J Huang M Bueber 《上海精神医学》2012,24(6):305-321
The following document is a translation of the first national mental health law of the People''s Republic of China, which was adopted by the National People''s Congress on October 26, 2012. The original Chinese version of the law is available at the official government website: http://www.gov.cn/jrzg/2012-10/26/content_2252122.htm. The translation was completed by a team of translators at the Shanghai Mental Health Center at the Shanghai Jiao Tong University School of Medicine. The translators have added footnotes at the end of the document that explain their choices in sections where alternative translations are possible and that provide background information for sections that may be confusing to readers unfamiliar with China. This translation should be cited as follows:ChenHH, Phillips MR, Cheng H, Chen QQ, Chen XD, Fralick D, Zhang YE, Liu M, Huang J, Bueber M. Mental Health Law of the People''s Republic of China (English translation with annotations). Shanghai Archives of Psychiatry. 2012; 24(6):305-321. doi:10.3969/j.issn.1002-0829.2012.06.001Shanghai Archives of Psychiatry 相似文献
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目的:用HPLC和电荷转移络合UV法分别测定特非那丁粒剂的含量,并对测定结果进行比较,方法:HPLC法,色谱柱:SpherisorbC8(150*4.6mm,5μm);流动相:甲醇-0.1mol/L三乙胺磷酸缓冲液(80:20),检测波长235nm。电荷转移络合UV法,利用碘与TFN在氯仿中形成电荷转移络合物的原理,在紫外294nm的波长处测定TFN的含量。结果:两法测定的线性范围和平均回收率分别 相似文献
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