van Rijk MC  Tanis PJ  Nieweg OE  Olmos RA  Rutgers EJ  Hoefnagel CA  Kroon BB 《Journal of surgical oncology》2006,94(4):281-286

PURPOSE: The aim of this study was to determine the incidence, location, surgical identification rate, tumor status, and clinical implications of sentinel nodes outside the axilla and internal mammary chain. PATIENTS AND METHODS: In 785 breast cancer patients, pre-operative lymphoscintigraphy was performed after intratumoral injection of 116 MBq 99mTc-labeled nanocolloid (0.2 ml; 3.1 mCi). Sentinel nodes were pursued using a gamma-ray detection probe and vital blue dye. RESULTS: Lymphoscintigraphy visualized sentinel nodes outside the axilla and internal mammary chain in 91 of the 785 patients (12%). Sentinel nodes (106) were identified in 80 patients. These nodes were found in the following locations: 50 in the breast, 31 in the infraclavicular fossa, 19 between the pectoral muscles, and 6 within the supraclavicular bed. Eighteen nodes contained a metastasis (17%) and were removed from 16 patients. The treatment strategy was adjusted in 12 of them with the addition of adjuvant local or systemic therapy. Two additional patients with an unusually situated tumor-negative sentinel node were spared an axillary node dissection that would otherwise have been performed. CONCLUSION: Unusually situated sentinel nodes were visualized in 12% of the patients. The treatment was adjusted in 18% of patients in whom these nodes were identified.  相似文献   

    

Guit  GL; Shaw  PC; Ehrlich  J; Kroon  HM; Oudkerk  M 《Radiology》1985,154(2):305-306

A case of mixed connective tissue disease (MCTD) is presented in which mediastinal lymphadenopathy was the most prominent radiological finding detected by plain chest radiographs and computed tomography. Pulmonary arterial hypertension, which is a rare and often fatal complication of MCTD, also developed in this patient.  相似文献   

  总被引：2，自引：0，他引：2  

O E Nieweg  P J Tanis  J D de Vries  B B Kroon 《Journal of surgical oncology》2001,78(4):223-224

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J A van Dongen  B B Kroon 《European journal of surgical oncology》1988,14(2):89-90

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Sexual issues among cervical cancer survivors: how can we help women seek help?     

Willemijn M. Vermeer  Rinske M. Bakker  Gemma G. Kenter  Cor D. de Kroon  Anne M. Stiggelbout  Moniek M. ter Kuile 《Psycho-oncology》2015,24(4):458-464

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Mazaheri Y  Bokacheva L  Kroon DJ  Akin O  Hricak H  Chamudot D  Fine S  Koutcher JA 《Journal of magnetic resonance imaging : JMRI》2010,32(5):1149-1157

Purpose

To present a semi‐automatic deformable registration algorithm for co‐registering T2‐weighted (T2w) images of the prostate with whole‐mount pathological sections of prostatectomy specimens.

Materials and Methods

Twenty‐four patients underwent 1.5 Tesla (T) endorectal MR imaging before radical prostatectomy with whole‐mount step‐section pathologic analysis of surgical specimens. For each patient, the T2w imaging containing the largest area of tumor was manually matched with the corresponding pathologic slice. The prostate was co‐registered using a free‐form deformation (FFD) algorithm based on B‐splines. Registration quality was assessed through differences between prostate diameters measured in right–left (RL) and anteroposterior (AP) directions on T2w images and pathologic slices and calculation of the Dice similarity coefficient, D, for the whole prostate (WP), the peripheral zone (PZ) and the transition zone (TZ).

Results

The mean differences in diameters measured on pathology and MR imaging in the RL direction and the AP direction were 0.49 cm and ?0.63 cm, respectively, before registration and 0.10 cm and ?0.11 cm, respectively, after registration. The mean D values for the WP, PZ and TZ, were 0.76, 0.65, and 0.77, respectively, before registration and increased to 0.91, 0.76, and 0.85, respectively, after registration. The improvements in D were significant for all three tissues (P < 0.001 for all).

Conclusion

The proposed semi‐automatic method enabled successful co‐registration of anatomical prostate MR images to pathologic slices. J. Magn. Reson. Imaging 2010;32:1149–1157. © 2010 Wiley‐Liss, Inc.

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Van der Bijl C  Alant E  Lloyd L 《Research in developmental disabilities》2006,27(1):43-55

The aim of this research study was to compare two strategies of sight word instruction in children attending a school for learners with moderate to severe mental disability, namely modified orthography (MO) and modified orthography where an association was made between the modification and the traditional orthography (MO/TO) together with a control group (TO). Thirty-three participants were matched according to their gender, receptive language skills and alphabet knowledge and assigned to the three groups, after which they were taught 10 sight words by using one of the above-mentioned strategies for 2 weeks. Word identification scores were obtained prior to teaching, on a daily basis during teaching, and after 3 weeks of withdrawal to determine the retention of identified words. Results show that individuals with moderate to severe mental disability are able to learn sight words through any of the three strategies implemented. Although there was no statistically significant difference between the three groups on a 5% level, significance on a 10% level was recorded for the MO/TO and TO groups. The order of effectiveness as measured by group averages on word identification was: MO/TO, TO and MO. The clinical implication of these results is that by using MO/TO as teaching strategy for sight words, individuals with limited literacy skills would be able to derive meaning from the written word while forming an association between the modification and the orthography. This could provide early reading success and enhance word identification.  相似文献   

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Titanji K  De Milito A  Cagigi A  Thorstensson R  Grützmeier S  Atlas A  Hejdeman B  Kroon FP  Lopalco L  Nilsson A  Chiodi F 《Blood》2006,108(5):1580-1587

Circulating memory B cells are severely reduced in the peripheral blood of HIV-1-infected patients. We investigated whether dysfunctional serologic memory to non-HIV antigens is related to disease progression by evaluating the frequency of memory B cells, plasma IgG, plasma levels of antibodies to measles, and Streptococcus pneumoniae, and enumerating measles-specific antibody-secreting cells in patients with primary, chronic, and long-term nonprogressive HIV-1 infection. We also evaluated the in vitro production of IgM and IgG antibodies against measles and S pneumoniae antigens following polyclonal activation of peripheral blood mononuclear cells (PBMCs) from patients. The percentage of memory B cells correlated with CD4+ T-cell counts in patients, thus representing a marker of disease progression. While patients with primary and chronic infection had severe defects in serologic memory, long-term nonprogressors had memory B-cell frequency and levels of antigen-specific antibodies comparable with controls. We also evaluated the effect of antiretroviral therapy on these serologic memory defects and found that antiretroviral therapy did not restore serologic memory in primary or in chronic infection. We suggest that HIV infection impairs maintenance of long-term serologic immunity to HIV-1-unrelated antigens and this defect is initiated early in infection. This may have important consequences for the response of HIV-infected patients to immunizations.  相似文献   

    

Depaz I  Ito M  Matsumoto I  Niwa S  Kroon P  Wilce PA 《Alcoholism, clinical and experimental research》2003,27(9):1481-1488

BACKGROUND: Human neuronal protein (hNP22) is a gene with elevated messenger RNA expression in the prefrontal cortex of the human alcoholic brain. hNP22 has high homology with a rat protein (rNP22). These proteins also share homology with a number of cytoskeleton-interacting proteins. METHODS: A rabbit polyclonal antibody to an 18-amino acid epitope was produced for use in Western and immunohistochemical analysis. Samples from the human frontal and motor cortices were used for Western blots (n = 10), whereas a different group of frontal cortex and hippocampal samples were obtained for immunohistochemistry (n = 12). RESULTS: The hNP22 antibody detected a single protein in both rat and human brain. Western blots revealed a significant increase in hNP22 protein levels in the frontal cortex but not the motor cortex of alcoholic cases. Immunohistochemical studies confirmed the increased hNP22 protein expression in all cortical layers. This is consistent with results previously obtained using Northern analysis. Immunohistochemical analysis also revealed a significant increase of hNP22 immunoreactivity in the CA3 and CA4 but not other regions of the hippocampus. CONCLUSIONS: It is possible that this protein may play a role in the morphological or plastic changes observed after chronic alcohol exposure and withdrawal, either as a cytoskeleton-interacting protein or as a signaling molecule.  相似文献   

    

D&  aacute  niel Szűcs  N&  oacute  ra Judit B&  eacute  res  R&  eacute  ka Rokonay  Kriszta Boros  Katalin Borka  Zolt&  aacute  n Kiss  r&  aacute  s Arat&  oacute  Attila J Szab&  oacute  &  Aacute  d&  aacute  m Vannay  Erna Sziksz  Csaba Bereczki  G&  aacute  bor Veres 《World journal of gastroenterology : WJG》2016,22(26):6027-6035

AIM: To evaluate the role of microRNA (miR)-146a, -155 and -122 in the duodenal mucosa of pediatric patients with Crohn’s disease (CD) and the effect of transforming growth factor-β (TGF-β) on these miRs in duodenal epithelial and fibroblast cells.METHODS: Formalin-fixed, paraffin-embedded biopsies derived from the macroscopically inflamed (CD inflamed: n = 10) and intact (CD intact: n = 10) duodenal mucosa of pediatric CD patients and control children (C: n = 10) were examined. Expression of miR-146a, -155 and -122 was determined by real-time polymerase-chain reaction (PCR). The expression of the above miRs was investigated in recombinant human TGF-β (1 nmol/L, 24 h) or vehicle treated small intestinal epithelial cells (CCL-241) and primary duodenal fibroblast cells derived from healthy children as well.RESULTS: Expression of miR-146a was significantly higher in the inflamed duodenal mucosa compared to the intact duodenal mucosa of children with CD (CD inflamed: 3.21 ± 0.50 vs CD intact: 0.62 ± 0.26, P≤ 0.01) and to the control group (CD inflamed: 3.21 ± 0.50 vs C: 1.00 ± 0.33, P≤ 0.05). The expression of miR-155 was significantly increased in the inflamed region of the duodenum compared to the control group (CD inflamed: 4.87 ± 1.02 vs Control: 1.00 ± 0.40, P≤ 0.001). The expression of miR-122 was unchanged in the inflamed or intact mucosa of CD patients compared to controls. TGF-β treatment significantly decreased the expression of miR-155 in small intestinal epithelial cells (TGF-β: 0.7 ± 0.083 vs Control: 1 ± 0.09, P≤ 0.05) and also the expression of miR-146a (TGF-β: 0.67 ± 0.04 vs Control: 1 ± 0.15, P≤ 0.01) and miR-155 (TGF-β: 0.72 ± 0.09 vs Control: 1 ± 0.06, P≤ 0.05) in primary duodenal fibroblasts compared to corresponding vehicle treated controls. TGF-β treatment did not influence the expression of miR-122.CONCLUSION: The elevated expression of miR-146a and -155 in the inflamed duodenal mucosa of CD patients suggests the role of these miRs in the pathomechanism of inflammatory bowel disease. Anti-inflammatory TGF-β plays an important role in the regulation of the expression of these miRs.  相似文献